scholarly journals Penggunaan Imunostik sebagai Uji Serologi untuk Deteksi Brucella abortus pada Sapi (APPLICATION IMMUNOSTICK ASSAY FOR SEROLOGICAL TEST BRUCELLA ABORTUS IN BOVINE)

2018 ◽  
Vol 19 (2) ◽  
pp. 169
Author(s):  
Dhevie Kenny Astarina ◽  
Eko Sugeng Pribadi ◽  
Fachriyan Hasmi Pasaribu
Keyword(s):  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Serological Test ◽  
Rapid Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Unknown Status ◽  
Cattle Serum ◽  
Two Stages ◽  
Elisa Result

Serological test is one of diagnostic method to detect pathogenicity brucella.  Several methods are being improving such as Rose Bengal Test (RBT), Complement Fixation Test (CFT) dan Enzyme-linked Immunosorbent Assay (ELISA). Immunostick has an accuracy equivalent to ELISA and is easy to apply in the field so it is possible to be applied as a rapid test for brucellosis detection. The study aim was to know sensitivity and specificity of immunostick that were used to detecte antibody Brucella abortus using commercial antigens of B. abortus Strain 19 (S19) and B. abortus Strain 99 (S99). The test have compared with ELISA. The tests were conducted in two stages, namely (i) immunostick ability to detect antibodies in seropositive and seronegative serum, and (ii) the immunostick result were compared with ELISA result in serum grup that were be know and unknown status. A total of 250 serums were examined and result indicated that immunostick can be detect B. abortus antibodies in cattle serum with sensitivity 100%. Immunostick specifity were 45,45% for B. abortus S99(1) antigen; 78,79% for B. abortus S99(2) antigen and 51,52% for B. abortus S19 antigen. When the test compared with ELISA, the sensitivity 82,86% and the spesifity were 52,31% for B. abortus S99(1) antigen; 93,54% and 79,71 for B. abortus S99(2) antigen and 82,86%  and 58,46% for B. abortus S19 antigen. 

scholarly journals Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis

1976 ◽  
Vol 77 (3) ◽  
pp. 369-376 ◽  
Author(s):  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught ◽  
M. J. Dalling ◽  
G. S. Allan
Keyword(s):  
Specific Antibody ◽  
Brucella Abortus ◽  
Bovine Serum ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Serological Test ◽  
Agglutination Test ◽  
Fixation Test ◽  
Bovine Brucellosis ◽  
Serum Agglutination

SUMMARYA radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to 1gM, a characteristic which may make it more suitable than the complement fixation test (OFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

scholarly journals Protective Effect of the Nramp1 BB Genotype against Brucella abortus in the Water Buffalo (Bubalus bubalis)

2006 ◽  
Vol 75 (2) ◽  
pp. 988-996 ◽  
Author(s):  
Rosanna Capparelli ◽  
Flora Alfano ◽  
Maria Grazia Amoroso ◽  
Giorgia Borriello ◽  
Domenico Fenizia ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Untranslated Region ◽  
Complement Fixation Test ◽  
Water Buffalo ◽  
Complement Fixation ◽  
Bubalus Bubalis ◽  
Lower Number ◽  
Agglutination Test ◽  
Intracellular Bacteria ◽  
Antibacterial Protein

ABSTRACT We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3′ untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

An Indirect Microimmunofluorescence Test for Detection of Chlamydial Antibodies in Ovine Fetal Fluids

1994 ◽  
Vol 6 (3) ◽  
pp. 315-320 ◽  
Author(s):  
T. P. Sanderson ◽  
A. A. Andersen ◽  
L. D. Miller ◽  
J. J. Andrew ◽  
B. H. Janke ◽  
...  
Keyword(s):  
Chlamydial Infection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Rapid Test ◽  
Chlamydia Psittaci ◽  
Diagnostic Laboratory ◽  
Specific Antibodies ◽  
Ovine Fetal ◽  
Microimmunofluorescence Test ◽  
Fetal Fluids

The objective of this study was to evaluate an indirect microimmunofluorescence test (IMIF) for detection of Chlamydial antibodies in serum and/or thoracic fluids of aborted ovine fetuses. One hundred eighty-two ovine fetuses, including 64 fetuses from 40 ewes that were experimentally infected with an ovine abortion strain of Chlamydia psittaci at gestation days 90–100, 10 fetuses from 6 normal ewes, and 108 fetuses selected from those received at the Iowa Veterinary Diagnostic Laboratory, were evaluated in this study. Fetuses from experimentally infected ewes were examined 4–60 days after inoculation. The IMIF findings were compared with the results of complement fixation serology for Chlamydiae and concentrations of immunoglobulin (IgG). Chlamydiae-specific antibodies were detected by IMIF in 28 of 38 fetuses infected with C. psittaci. Elevated levels of IgG and IMIF titers ≥ 1:8 were consistent findings in ovine fetuses infected with Chlamydiae for more than 24 days. IgG levels and titers of Chlamydial antibodies increased with maturity of the fetus and duration of Chlamydial infection. Chlamydial antibodies were not detected with the complement fixation test. Fluids from ovine fetuses aborted as a result of other causes also were examined, and IMIF results were negative. The results of this study indicate that the IMIF is a useful and relatively rapid test for identification of Chlamydial antibodies in ovine fetuses.

scholarly journals Complement Fixation Test To Assess Humoral Immunity in Cattle and Sheep Vaccinated with Brucella abortusRB51

1999 ◽  
Vol 6 (6) ◽  
pp. 787-790 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Humoral Immunity ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Deficient Mutant ◽  
Anticomplementary Activity ◽  
Bovine Brucellosis ◽  
Serologic Tests ◽  
Rough Strain ◽  
Cattle And Sheep

ABSTRACT The live attenuated Brucella abortus strain RB51 is a rifampin-resistant, lipopolysaccharide (LPS) O-chain-deficient mutant of virulent B. abortus 2308. The reduced O-chain content in RB51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to LPS. The absence of available serologic tests for RB51 also complicates the diagnosis of possible RB51 infections in humans exposed to this strain. The purpose of this study was to evaluate the suitability of a complement fixation (CF) test performed with the rough strain B. abortus RB51, previously deprived of anticomplementary activity, in detecting anti-B. abortus RB51 antibodies in cattle and sheep experimentally vaccinated with this strain. The results of this study showed that a CF test with RB51 as the antigen is able to specifically detect antibodies following RB51 vaccination in cattle and sheep. In addition, this method could be a useful tool for detecting B. abortus RB51 infection in humans.

scholarly journals The development and evaluation of a μ-capture ELISA detecting chlamydia-specific Igm

1988 ◽  
Vol 101 (2) ◽  
pp. 387-395 ◽  
Author(s):  
T. G. Wreghitt ◽  
V. J. Robinson ◽  
E. O Caul ◽  
I. D. Paul ◽  
S. Gatley
Keyword(s):  
Respiratory Tract ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Specific Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Capture Elisa ◽  
The Past ◽  
Heat Stable ◽  
Antibody Conjugate

SUMMARYA μ-capture enzyme-linked immunosorbent assay (ELISA) for detecting chlamydia-specific IgM was developed by use of the heat stable, lipopolysaccharide group-specific antigen and an alkaline phosphatase-labelled anti-chlamydia group-specific monoclonal antibody conjugate. The test was used to study the serological response in chlamydial respiratory tract infection among patients with acute respiratory tract symptoms in Cambridgeshire during the past 7 years. Results were compared with those of the complement fixation test (CFT) in routine use as well as those of a whole inclusion indirect immunofluorescence (WIF) test for IgM. Correlation between results of the μ-capture ELISA and those of the WIF test was 87·5%.

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