scholarly journals Protective Effect of the Nramp1 BB Genotype against Brucella abortus in the Water Buffalo (Bubalus bubalis)

2006 ◽  
Vol 75 (2) ◽  
pp. 988-996 ◽  
Author(s):  
Rosanna Capparelli ◽  
Flora Alfano ◽  
Maria Grazia Amoroso ◽  
Giorgia Borriello ◽  
Domenico Fenizia ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Untranslated Region ◽  
Complement Fixation Test ◽  
Water Buffalo ◽  
Complement Fixation ◽  
Bubalus Bubalis ◽  
Lower Number ◽  
Agglutination Test ◽  
Intracellular Bacteria ◽  
Antibacterial Protein

ABSTRACT We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3′ untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

scholarly journals The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests

1982 ◽  
Vol 88 (1) ◽  
pp. 11-19 ◽  
Author(s):  
R. J. Chappel ◽  
J. Hayes ◽  
B. A. Rogerson ◽  
L. J. Shenfield
Keyword(s):  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Serological Response ◽  
Serum Samples ◽  
Plate Test ◽  
The Rose ◽  
Serum Agglutination ◽  
Haemolysis Test

SummarySerum samples were obtained from 281 heifers vaccinated with Brucella abortus strain 19, and from 50 heifers that had received two injections of killed B. abortus strain 45/20 adjuvant (K45/20A) vaccine. The serological response measured by the brucellosis radioimmunoassay (RIA) was compared with responses measured by other tests.The serological responses of cattle during the first weeks after strain 19 vaccination were found to give little guide to the frequency of persistent reactions.In the case of strain 19, persistent reactions were considered to be those occurring 12 or more months after vaccination. In heifers vaccinated at the recommended age, small numbers of persistent reactions were given by the RIA (four in 374 sera), the complement fixation test using warm fixation (CFTW) (six in 383) and cold fixation (one in 185), the serum agglutination test (two in 222) and the indirect haemolysis test (IHLT) (two in 369). The Rose Bengal plate test gave 74 persistent reactions in 374 sera.Five of the 50 heifers gave particularly prolonged responses to K45/20A vaccine. In these animals the RIA and IHLT remained positive for longer than the CFTW.

scholarly journals Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis

1976 ◽  
Vol 77 (3) ◽  
pp. 369-376 ◽  
Author(s):  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught ◽  
M. J. Dalling ◽  
G. S. Allan
Keyword(s):  
Specific Antibody ◽  
Brucella Abortus ◽  
Bovine Serum ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Serological Test ◽  
Agglutination Test ◽  
Fixation Test ◽  
Bovine Brucellosis ◽  
Serum Agglutination

SUMMARYA radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to 1gM, a characteristic which may make it more suitable than the complement fixation test (OFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Detection of brucellosis in cattle and evaluation of risk factors associated with the disease in workers slaughterhouse

2021 ◽  
Vol 10 (3) ◽  
pp. e39710313248
Author(s):  
Thaise Marques Alves ◽  
Poliana de Castro Melo ◽  
Lilia Marcia Paulin Silva ◽  
Nathana Kyolla Santos de Carvalho ◽  
Amora Ferreira Menezes Rios ◽  
...  
Keyword(s):  
Risk Factors ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
State Inspection ◽  
Agglutination Test ◽  
The State ◽  
Antigen Test ◽  
Factors Associated ◽  
Inspection Service ◽  
Smooth Strain

Brucellosis is anthropozoonosis caused by Brucella spp. Among the zoonotic species, B. abortus is the main species affecting cattle and can easily be transmitted to humans. The purpose of this study was to investigate, through epidemiological inquiry and serological analysis,  animal and human health as related to smooth strain Brucella spp. in a slaughterhouse located in the southern region of the state of Bahia. For this purpose, blood samples were collected from workers and animals at a slaughterhouse together with the State Inspection Service. Then, the Buffered Acidified Plate Antigen test was performed for animals and humans, the Slow Agglutination Test was performed for humans only; and the Complement Fixation Test and the 2-Mercaptoethanol Brucella Agglutination test (2ME) were performed for animals only. In addition, an epidemiological inquiry was applied to workers in order to assess risk factors for the disease. After data analysis, it was concluded that infection by smooth strains of Brucella spp. was detected in 14.0% of the cattle. Additionally, one worker out 41 tested reactive to the disease.

scholarly journals Complement Fixation Test To Assess Humoral Immunity in Cattle and Sheep Vaccinated with Brucella abortusRB51

1999 ◽  
Vol 6 (6) ◽  
pp. 787-790 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Humoral Immunity ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Deficient Mutant ◽  
Anticomplementary Activity ◽  
Bovine Brucellosis ◽  
Serologic Tests ◽  
Rough Strain ◽  
Cattle And Sheep

ABSTRACT The live attenuated Brucella abortus strain RB51 is a rifampin-resistant, lipopolysaccharide (LPS) O-chain-deficient mutant of virulent B. abortus 2308. The reduced O-chain content in RB51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to LPS. The absence of available serologic tests for RB51 also complicates the diagnosis of possible RB51 infections in humans exposed to this strain. The purpose of this study was to evaluate the suitability of a complement fixation (CF) test performed with the rough strain B. abortus RB51, previously deprived of anticomplementary activity, in detecting anti-B. abortus RB51 antibodies in cattle and sheep experimentally vaccinated with this strain. The results of this study showed that a CF test with RB51 as the antigen is able to specifically detect antibodies following RB51 vaccination in cattle and sheep. In addition, this method could be a useful tool for detecting B. abortus RB51 infection in humans.

scholarly journals A serological prevalence survey of Brucella abortus in cattle of rural communities in the province of KwaZulu-Natal, South Africa : article

2008 ◽  
Vol 79 (1) ◽  
Author(s):  
U.W. Hesterberg ◽  
R. Bagnall ◽  
K. Perrett ◽  
B. Bosch ◽  
R. Horner ◽  
...  
Keyword(s):  
Rural Communities ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Primary Sampling Unit ◽  
Prevalence Survey ◽  
Test Sensitivity ◽  
Apparent Prevalence ◽  
Kwazulu Natal ◽  
The Rose

A serological survey of Brucella abortus in cattle originating from communal grazing areas of Kwa Zulu Natal was carried out between March 2001 and December 2003. The survey was designed as a 2-stage survey, considering the diptank as the primary sampling unit. In total 46 025 animals from 446 diptanks of 33 magisterial districts were sampled and tested using the Rose Bengal test and Complement Fixation Test. The apparent prevalence at district level was adjusted for clustering, diagnostic test sensitivity and specificity, and mapped using ArcView version 3.3. The prevalence of brucellosis in communal grazing areas of Kwa-Zulu Natal was found to be 1.45 % (0.84-2.21 %) and varied from 0 to 15.6% between magisterial districts. In 19 of the 33 magisterial districts no serological reactors were observed. A large variation in prevalence was found within diptank areas. Brucellosis was found to be most prevalent in the northeastern area of the province. The findings of the survey are discussed.

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