An Indirect Microimmunofluorescence Test for Detection of Chlamydial Antibodies in Ovine Fetal Fluids

1994 ◽  
Vol 6 (3) ◽  
pp. 315-320 ◽  
Author(s):  
T. P. Sanderson ◽  
A. A. Andersen ◽  
L. D. Miller ◽  
J. J. Andrew ◽  
B. H. Janke ◽  
...  
Keyword(s):  
Chlamydial Infection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Rapid Test ◽  
Chlamydia Psittaci ◽  
Diagnostic Laboratory ◽  
Specific Antibodies ◽  
Ovine Fetal ◽  
Microimmunofluorescence Test ◽  
Fetal Fluids

The objective of this study was to evaluate an indirect microimmunofluorescence test (IMIF) for detection of Chlamydial antibodies in serum and/or thoracic fluids of aborted ovine fetuses. One hundred eighty-two ovine fetuses, including 64 fetuses from 40 ewes that were experimentally infected with an ovine abortion strain of Chlamydia psittaci at gestation days 90–100, 10 fetuses from 6 normal ewes, and 108 fetuses selected from those received at the Iowa Veterinary Diagnostic Laboratory, were evaluated in this study. Fetuses from experimentally infected ewes were examined 4–60 days after inoculation. The IMIF findings were compared with the results of complement fixation serology for Chlamydiae and concentrations of immunoglobulin (IgG). Chlamydiae-specific antibodies were detected by IMIF in 28 of 38 fetuses infected with C. psittaci. Elevated levels of IgG and IMIF titers ≥ 1:8 were consistent findings in ovine fetuses infected with Chlamydiae for more than 24 days. IgG levels and titers of Chlamydial antibodies increased with maturity of the fetus and duration of Chlamydial infection. Chlamydial antibodies were not detected with the complement fixation test. Fluids from ovine fetuses aborted as a result of other causes also were examined, and IMIF results were negative. The results of this study indicate that the IMIF is a useful and relatively rapid test for identification of Chlamydial antibodies in ovine fetuses.

scholarly journals Enzyme-linked immunosorbent assay for chlamydial antibodies

1977 ◽  
Vol 6 (5) ◽  
pp. 507-510
Author(s):  
V J Lewis ◽  
W L Thacker ◽  
S H Mitchell
Keyword(s):  
Immunosorbent Assay ◽  
Chlamydial Infection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Chlamydia Psittaci ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Sera ◽  
History Of ◽  
Species Specific ◽  
Formalin Fixed

An enzyme-linked immunosorbent assay (ELISA) detected chlamydial antibodies in human sera. The assay antigen produced in cell cultures infected with Chlamydia psittaci was Formalin-fixed to microplates. Single convalescent-phase sera positive for chlamydial antibodies by a complement-fixation test were positive at even higher dilutions by ELISA. Paired sera with diagnostic rises in complement-fixing antibody showed seroconversion by ELISA also. Control sera from persons with no history of chlamydial infection were negative by both tests. Sera from patients with psittacosis or lymphogranuloma venereum were ELISA positive, indicating that the assay with the antigen used in this study is genus specific rather than species specific.

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Seroprevalence of Bovine Anaplasmosis in Oklahoma from 1977 to 1991

1994 ◽  
Vol 6 (2) ◽  
pp. 200-206 ◽  
Author(s):  
S. J. Rodgers ◽  
R. D. Welsh ◽  
M. E. Stebbins
Keyword(s):  
Geographic Distribution ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Anaplasma Marginale ◽  
Fixation Test ◽  
Animal Disease ◽  
Diagnostic Laboratory ◽  
Bovine Anaplasmosis

The prevalence of anaplasmosis in Oklahoma cattle was determined on the basis of the standardized Anaplasma marginale complement fixation test on 20,155 sera submitted to the Oklahoma Animal Disease Diagnostic Laboratory during a 15-year period. Rates of seropositivity ranged from 4.7% to 17.6% on samples submitted for anaplasmosis testing of adult cows. The geographic distribution of recorded cases of anaplasmosis was 35 Oklahoma counties in 1977 and 48 Oklahoma counties in 1991.

STUDIES ON COMPLEMENT FIXING ANTIGENS OF LEPTOSPIRAE

1960 ◽  
Vol 6 (4) ◽  
pp. 453-462 ◽  
Author(s):  
N. A. Labzoffsky ◽  
A. E. Kelen
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Specific Antigen ◽  
Cross Reaction ◽  
Fixation Test ◽  
Specific Antibodies ◽  
Cross Reactions ◽  
Homologous Antigen ◽  
Precise Diagnosis ◽  
Specific Antigens

Methods of preparation of "whole" and type-specific antigens from leptospiral cultures for use in the complement fixation test are outlined. Leptospiral cultures grown in Korthof's medium were treated with pyridine and after appearance of a copious precipitate were centrifuged. The supernatants were dialyzed and after concentration by pervaporation were used as "whole" antigens. Type-specific antigens were prepared from acetone precipitates of filtrates of "whole" antigens. Considerable cross reaction was observed with "whole" antigens prepared from L. pomona, L. canicola, and L. icterohaemorrhagiae and their respective rabbit hyperimmune sera, although titers with homologous sera were invariably higher. These cross reactions, however, were not as pronounced with sera from cattle naturally infected with L. pomona, where approximately 43% of the sera reacted with homologous antigen only. No reactions were observed between the type-specific antigen and any of the heterologous sera employed. It has also been shown that with experimental sera type-specific antibodies appeared earlier and persisted longer than group-specific antibodies. In view of the above observations the use of polyvalent antigen for routine screening of leptospiral antisera is advocated. Type-specific antigens are recommended for use in more precise diagnosis.

Experimente zur Erzeugung tumorspezifischer, zirkulierender Antikörper

1960 ◽  
Vol 15 (6) ◽  
pp. 375-378
Author(s):  
Arno Geissler ◽  
Wolfgang Müller-Ruchholtz ◽  
Else Knake
Keyword(s):  
Wistar Rats ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Tissue Cultures ◽  
Agar Gel ◽  
Specific Antibodies ◽  
Cytotoxic Antibodies ◽  
Precipitin Test ◽  
Gel Diffusion

The immunization of highly inbred Wistar rats with a Benzpyrene-Sarcoma produced in this strain did not stimulate the formation of circulating tumor-specific antibodies. In these studies the complement fixation test, the colloid-complement fixation test, the ring precipitin test, the agar-gel-diffusion technic of Ouchterlony and tissue cultures for the study of cytotoxic antibodies were used.

scholarly journals Seroprevalence of antibodies to chlamydophila abortus shown in awassi sheep and local goats in jordan

2012 ◽  
Vol 49 (No. 12) ◽  
pp. 460-466 ◽  
Author(s):  
K. M Al-Qudah ◽  
L. A Sharif ◽  
R. Y Raouf ◽  
N. Q Hailat ◽  
F. M Al-Domy
Keyword(s):  
Chlamydial Infection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Strong Association ◽  
Flock Size ◽  
Infection Rates ◽  
Awassi Sheep ◽  
Seropositive Animal ◽  
Significant Difference ◽  
Northern Jordan

A cold complement fixation test (CFT) was used to identify C. abortus infection in ewes and does in northern Jordan. Sera from 36 flocks of sheep and 20 flocks of goats were collected randomly. The results showed that 433 (21.8%) out of 1 984 ovine sera, and 82 (11.4%) out of 721 caprine sera, were seropositive for C. abortus infection, as indicated by a titre ≥ 1:40. However, all the tested sheep flocks and goat flocks (100%) revealed at least one seropositive animal. There was a strong association (P < 0.05) between the rate of C. abortus infection and the size of the sheep flock, when larger flocks had higher infection rates. However, in goats, the flock size had no relationship with seropositivity. Age had no significant (P > 0.05) impact on C. abortus seropositivity. In sheep, there was a significant difference (P < 0.05) between the rates of the chlamydial infection in the four studied areas of northern Jordan. The highest infection rate in sheep (31.2%) was recorded in Mafraq area, while the rates in Irbid, Ajloun and Jerash were 18.5%, 11.2% and 13.9%, respectively. In goats, there was no significant difference between the rates of the chlamydial infection in the two areas studied. The rates of goat infections were 10.8% and 11.8% in Ajloun and Jerash areas, respectively.

scholarly journals Brucellosis and chlamydiosis seroprevalence in goats at livestock–wildlife interface areas of Zimbabwe

2019 ◽  
Vol 86 (1) ◽  
Author(s):  
Solomon Bhandi ◽  
Davies M. Pfukenyi ◽  
Gift Matope ◽  
Absolom Murondoti ◽  
Musavengana Tivapasi ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Chlamydial Infection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Flock Size ◽  
Serological Evidence ◽  
Interface Area ◽  
Domestic Ruminants ◽  
The Rose

In Zimbabwe, there have been no chlamydiosis and limited brucellosis studies in goats. This study was conducted to determine the seroprevalence and risk factors of the two diseases in goats at three different livestock–wildlife interface areas: porous, non-porous and non-interface in the south-eastern lowveld of Zimbabwe. Collected sera (n = 563) were tested for Brucella antibodies using the Rose Bengal plate test (RBPT) and the complement fixation test (CFT); and for Chlamydia abortus antibodies using the CFT. All tested goats were negative for Brucella antibodies. Overall, chlamydial seroprevalence was 22%. The porous [c2 = 9.6, odds ratio (OR) = 2.6, p = 0.002] and non-porous (c2 = 37.5, OR = 5.8, p < 0.00001) interfaces were approximately three and six times more likely to be chlamydial seropositive than the non-interface area, respectively. Chlamydial seroprevalence was not associated with sex (c2 = 0.5, OR = 1.2, p = 0.5), abortion history in female goats (c2 = 0.7, OR = 1.3, p = 0.4), keeping goats with cattle (c2 = 0.2, OR = 1.5, p = 0.7) or flock size (c2 = 0.03, OR = 1.4, p = 0.9). Our study provides the first serological evidence of chlamydiosis in goats in Zimbabwe and the results suggest that proximity to wildlife is associated with increased chlamydial seropositivity. Further studies are required to determine the role of chlamydial infection on goat reproductive failure and that of wildlife on C. abortus transmission to domestic ruminants.

scholarly journals A study of human respiratory tract chlamydial infections in Cambridgeshire 1986–88

1990 ◽  
Vol 104 (3) ◽  
pp. 479-488 ◽  
Author(s):  
T. G Wreghitt ◽  
C. E Barker ◽  
J. D Treharne ◽  
J. M Phipps ◽  
V Robinson ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Chlamydia Pneumoniae ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Chlamydia Psittaci ◽  
Fixation Test ◽  
Serum Samples ◽  
Human Respiratory Tract ◽  
Relative Incidence ◽  
Chlamydial Infections

SUMMARYHuman respiratory tract chlamydial infections have been studied in Cambridge-shire for many years, but until recently we have been unable to distinguish between infection withChlamydia psittaciOrChlamydia pneumoniae(TWAR). In this study, we have employed the micro-immunofluorescence (micro-IF) test for this purpose and to look for the relative incidence ofC. psittaciandC. pneumoniaeinfections in Cambridgeshire. Among 50 patients with community-acquired respiratory tract symptoms whose serum samples had Chlamydia complement fixation test titres ≥ 64, 25 had evidence of recentC. psittaciorC. pneumoniaeinfection. Nineteen (76%) of the 25 patients had evidence of recentC. psittaciinfection and of these 16 (84%) had recently had contact with birds. Six patients (24%) had evidence of recentC. pneumoniaeinfection, and of these, only two (33% had recently had contact with birds). WhileC. psittaciwas grown from several of the birds associated with humanC. psittaciinfection, it was not cultured from any of the birds in contact with the two humanC. pnemoniaecases.

scholarly journals Serosurvey of Chlamydia psittaci Infections among Livestock and Human Beings Employing Complement Fixation Test

1997 ◽  
Vol 11 (2) ◽  
pp. 163-168 ◽  
Author(s):  
V. B. Joshi ◽  
R. C. Katoch ◽  
K. B. Nagal ◽  
M. K. Batta ◽  
R. K. Asrani ◽  
...  
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Chlamydia Psittaci ◽  
Fixation Test ◽  
Human Beings

scholarly journals Molluscum contagiosum: serology and electron microscopy findings in twenty one patients

1987 ◽  
Vol 29 (2) ◽  
pp. 86-89 ◽  
Author(s):  
M. E. F. Fonseca ◽  
R. D. Machado ◽  
M. I. M. Liberto ◽  
G. Marcolino
Keyword(s):  
Electron Microscopy ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Virus Disease ◽  
Molluscum Contagiosum ◽  
Fixation Test ◽  
Molluscum Contagiosum Virus ◽  
Serological Tests ◽  
Specific Antibodies ◽  
Electron Microscopical

Twenty one cases of molluscum contagiosum virus disease were collected for electron microscopical and serological tests. Molluscum virus was detected in the crust, inside the vacuoles formed in the keratinocytes cells. The patients developed specific antibodies to the virus detected by complement fixation test.

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