Evaluation of the Parameters Affecting the Success Rate of the Microfluidics-Based Sperm Selection for In Vitro Fertilization

<h1>Resumen </h1>Introducción: A pesar de los avances en las técnicas de reproducción asistida, la tasa de éxito en fertilización in vitro (FIV) sigue siendo modesta. La lesión endometrial, mediante histeroscopía o biopsia, previo a un ciclo de FIV, ha sido propuesta como una técnica que aumenta la probabilidad de implantación exitosa y embarazo en mujeres sometidas a FIV. Objetivo: Estudiar el efecto de la estimulación endometrial en la tasa de éxito de FIV. Métodos: Se realizó un estudio unicéntrico observacional, en pacientes sometidas a su primer ciclo de FIV. Se incluyeron mujeres entre las edades de 18-40 años que planeaban someterse a FIV con sus propios ovocitos sin exposición reciente a procedimientos disruptivos uterinos. Las pacientes elegibles se dividieron en 3 grupos. El primer grupo incluyó las que se sometieron a una histeroscopía diagnóstica previa a su ciclo de FIV, el segundo grupo incluyó aquellas que se sometieron a biopsia endometrial con cánula de Pipelle previo al ciclo de FIV y el tercer grupo incluyó a las pacientes sin intervención endometrial. Resultados: Se estudiaron un total de 68 pacientes (10 en el grupo 1, 27 en el grupo 2 y 31 en el grupo 3). La tasa de embarazo clínico fue estadísticamente similar en los tres grupos (33.3% para el primer grupo, 15.7% para el segundo y 16.6% para el tercero, p = 0.93). Conclusión: La estimulación endometrial antes del primer ciclo de FIV no ofreció beneficios adicionales en relación con implantación exitosa y/o tasas clínicas de embarazo. Palabras clave: lesión endometrial, fertilización in vitro, implantación, infertilidad, nacido vivo <h1>Abstract</h1>Background: Despite advances in assisted reproduction techniques, the in vitro fertilization (IVF) success rate still remains modest. Endometrial injury, through hysteroscopy or biopsy, prior to an IVF cycle, has been proposed as a technique that increases the likelihood of successful implantation and pregnancy in women undergoing IVF. Aim and Objective: To study the effect of endometrial stimulation on the success rate of IVF. Methods: An ambispective observational unicentric study was conducted in patients undergoing their first IVF cycle. Women between the ages of 18-40 who planned to undergo IVF with their own oocytes without recent exposure to uterine disruptive procedures were included. Eligible patients were divided into 3 groups. The first group included those who underwent a diagnostic hysteroscopy prior to their IVF cycle, the second group included those who underwent endometrial biopsy with a Pipelle cannula prior to the IVF cycle and the third group included patients without endometrial intervention. Results: A total of 68 patients were studied (10 in group 1, 27 in group 2 and 31 in group 3). The clinical pregnancy rate was statistically similar in the three groups (33.3% for the first group, 15.7% for the second group and 16.6% for the third group, p = 0.93). Conclusion: Endometrial stimulation before the first IVF cycle did not offer additional benefits in relation to successful implantation and / or clinical pregnancy ratesKeywords: endometrial injury, in vitro fertilization, implantation, infertility, live birth*****

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Luaran Transfer Embrio Simpan Beku pada Pasien Endometriosis Pasca Operasi dan Non Endometriosis yang Menjalani IVF di Klinik Permata Hati RSUP Dr. Sardjito

Jurnal Kesehatan Reproduksi ◽

10.22146/jkr.56940 ◽

2020 ◽

Vol 7 (2) ◽

pp. 108

Author(s):

Rina Fatmawati ◽

Shofwal Widad ◽

Agung Dewanto

Keyword(s):

In Vitro Fertilization ◽

Success Rate ◽

Embryo Transfer ◽

Pregnancy Outcomes ◽

Clinical Pregnancy ◽

Frozen Embryo Transfer ◽

Research Subjects ◽

Vitro Fertilization ◽

Biochemical Pregnancy

Background: Endometriosis is a chronic condition that is influenced by the hormone estrogen which affects women of childbearing age, and is associated with pelvic pain and infertility. In Vitro Fertilization (IVF) is currently the most efficient assisted reproductive technology and its high success rate is often done for infertility therapy in women associated with endometriosisObjective: The aim of this study is to determine whether postoperative endometriosis affected pregnancy outcomes in patients underwent frozen embryo transfer in IVF / ICSI programs.Method: This Research is done with a retrospective cohort design. The data was taken from medical records, research subjects who met the inclusion and exclusion criteria. The research data was collected, processed and analyzed using SPSS 23. Univariate, bivariate and multivariate data analysis was carried out to determine the effect between variablesResult: There were 458 research subjects in this study. Endometriosis patients were 119 subjects (26%). 57 subjects were categorized as minimum-mild endometriosis (47.9%) and moderate-severe subjects as many as 62 subjects (52.1%). The biochemical pregnancy rate (36.31%) and clinical pregnancy (29.4%) in patients with endometriosis was slightly higher than in non-endometriosis. But statistically it did not affect success rate of achieving biochemical (p = 0.428; RR 0.89; 95% CI: 0.71-1.24) and clinical pregnancy (p = 0.535; RR 0.883; 95% CI: 0.63- 1.22). The rate of miscarriage in postoperative endometriosis patients was higher than non-endometriosis patients (88.6% vs 80.7%) but was not statistically significant (p = 0.294; RR 1.69; 95% CI: 0.61-4.67) . Biochemical and clinical pregnancies were significantly affected by age, infertility, endometrial thickness, embryo age and embryo quality. The incidence of miscarriage was affected by the ovarian stimulation protocol.Conclusion: Endometriosis post operative statistically has no effect on pregnancy outcomes in the IVF / ICSI cycle with frozen embryo transfer compared with another cause of infertility .Keywords:Endometriosis, In Vitro Fertilization, Clinical pregnancy, biochemical pregnancy, miscarriage

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