scholarly journals Simultaneous spectrophotometric determination of phenolic antioxidant (BHA and BHT) concentrations in pharmaceutical preparations and chewing gums using the H-point standard addition method

2017 ◽  
Vol 36 (2) ◽  
pp. 211
Author(s):  
Mahmure Özgür ◽  
Zeynep Kalaycioğlu ◽  
Öznur Dülger
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Standard Addition ◽  
Butylated Hydroxytoluene ◽  
Standard Addition Method ◽  
Addition Method ◽  
Chewing Gums ◽  
Relative Standard

A simple, rapid, and sensitive spectrophotometric method was developed for the simultaneous determination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) concentration in pharmaceutical preparations and chewing gums, without prior separation steps, using the H-point standard addition method (HPSAM).The concentration of one antioxidant was calculated by overlapping spectra at two appropriately selected wavelengths at which the interferent, other antioxidant, should has the same absorbance value. Absorbances at two pairs of wavelengths, 265 and 288 nm (with BHA as analyte) or 288 and 293 nm (with BHT as analyte) were monitored, while adding standard solutions of BHA or BHT, respectively. Calibration graphs were determined at 4–20 μgml–1(r = 0.9981) for BHA and 20–100 μgml–1(r = 0.9940) for BHT in binary mixtures. The proposed method was tested and validated using various parameters according to ICH guidelines. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.48 and 1.51 μgml–1for BHA and 0.72 and 2.41 μgml–1for BHT, respectively. The percentage recovery ranges were 100.44–102.50 % for BHA and 96.45–100.04 % for BHT, with relative standard deviations (RSD) less than 1.48 % indicating reasonable repeatability of the method. The intra-day and inter-day precision tests showed reliable RSD values (< 2 %). The results obtained using HPSAM were statistically compared with results obtained using the derivative spectrophotometric method that was previously reported by us, showing high similarity between results.

scholarly journals MEAN CENTERING OF RATIO SPECTRA METHOD FOR THE ANALYSIS OF THEOPHYLLINE AND EPHEDRINE HCL MIXTURE IN TABLET

2018 ◽  
Vol 11 (5) ◽  
pp. 218
Author(s):  
Muchlisyam Muchlisyam ◽  
Sudarmi Sudarmi ◽  
Cindy Caroline
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Standard Addition ◽  
Addition Method ◽  
Limit Of Quantification ◽  
Mean Centering ◽  
Relative Standard

 Objective: Mean centering of ratio spectra method (MCR method) is one of the simplest methods for the determination of drug mixtures. The purpose of this research is to determine the content of theophylline (THEO) and ephedrine HCl (EPH) in tablets by MCR spectra method.Methods: This research was conducted with the MCR method. It was measured at 271 nm for THEO and 257 nm for EPH using 0.1 N HCL as a solution. The calculation was conducted with Matlab application. The analytical characteristics of the method are detection limit, accuracy, precision, and selectivity. Standard addition method was used to increase the concentration of EPH in the sample until it reached the range of calibration concentration.Result: The research has showed that validations for THEO were 100.57% for accuracy, 0.68% for relative standard deviation (RSD), 0.46 μg/mL for limit of detection (LOD), and 1.52 μg/mL for limit of quantification (LOQ). Meanwhile, the validations for EPH are 100.02% for accuracy, 0.07% for RSD, 43.12 μg/mL for LOD, and 143.72 μg/mL for LOQ. The level of THEO is 97.43 ± 0.17% and the level of EPH is 101.36 ± 0.25% for brand one’s tablet. Meanwhile, brand two’s tablet contains 98.72 ± 0.14% of THEO and 103.62 ± 0.23% of EPH.Conclusion: MCR ultraviolet spectrophotometric method can be used to determine the content of THEO and EPH in tablets and meets the detection limit, accuracy, precision, and selectivity.

scholarly journals H-point Standard Addition Method for the Simultaneous Spectrophotometric Determination of Captopril and Hydrochlorothiazide in Pharmaceutical Formulations

2021 ◽  
pp. 77-85
Keyword(s):  
Spectrophotometric Determination ◽  
Standard Addition ◽  
Pharmaceutical Formulations ◽  
Standard Addition Method ◽  
Addition Method ◽  
Real Samples ◽  
Relative Standard ◽  
Recovery Percentage ◽  
Precision And Accuracy

Simultaneous spectrophotometric determination of captopril and hydro-chlorothiazide in pharmaceutical formulations by the H-point standard addition method (HPSAM) is described. Absorbance at 211.5 and 230.3 nm was monitored upon the addition of standard solutions of captopril. The results showed that in prepared mixtures, captopril and hydrochlorothiazide can be determined simultaneously at concentration ratios varying from 1.0:0.8 to 2.5:1.5 μg/mL, respectively. Percentage recovery was found to be 95.33–104.37% for captopril and 96.8–105% for hydrochlorothiazide, with a relative standard deviation (RSD) of 2.46%. The method was successfully used to evaluate the antihypertensive captopril drug in a binary combination of hydrochlorothiazide in real samples with high precision and accuracy within the recovery percentage.

Spectrophotometric Method for the Assay of Chlorhexiidine Acetate

2014 ◽  
Vol 912-914 ◽  
pp. 321-324
Author(s):  
Yan Hong Lei
Keyword(s):  
Spectrophotometric Method ◽  
High Sensitivity ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Addition Method ◽  
Ultraviolet Spectrophotometry ◽  
Good Linear ◽  
Sensitivity And Selectivity

To establish the determination of the content of chlorhexiidine acetate by ultraviolet spectrophotometry .The spectra (range: λ=200~320 nm) were processed. The linear ranges of chlorhexidine acetate was at 2~ 20 μg mL-1 .the chlorhexiidine acetate showed good linear correlation.The ultraviolet spectrophotometry data of the samples were also used to evaluate the samples quantitative composition.It was shown that recovery of the method by standard addition method was respectively valued 99.55% for chlorhexiidine acetate .The method was simple,reliable,accurate,reproducible with high sensitivity and selectivity.

Quantitative Determination of Ga, Zn, Cu, Hi, Mn, and Cr by X-Ray Fluorescence in Laterites and Bauxites Using Two Evaluation Methods

1981 ◽  
Vol 25 ◽  
pp. 127-131
Author(s):  
Hasso Schorin
Keyword(s):  
Quantitative Determination ◽  
Chemical Weathering ◽  
Limit Of Detection ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Addition Method ◽  
X Ray ◽  
Minor Elements ◽  
Trace And Minor Elements

Laterites and bauxites are residual products derived from a wide variety of rocks by intensive chemical weathering under strongly oxidizing and leaching conditions. Generally the main constituents of these residues are Fe, Al and Ti present in form of hydroxides and/or oxides. The content of silica depends on the thoroughness of the leaching process. The behaviour of trace and minor elements as Ga, Zn, Cu, Ni, Mn and Cr during the laterite/bauxite formation is not yet well established. Precise and accurate analyses of these elements are a prerequisite for such investigations. In this paper the standard addition method is presented and the results are compared with those obtained by calibration standardization. The accuracy and the precision of the methods as well as the distinction limit and the limit of detection for each element are given.

Spectrophotometric Method for the Assay of Aspirin

2014 ◽  
Vol 602-605 ◽  
pp. 2097-2100
Author(s):  
Yan Hong Lei
Keyword(s):  
Spectrophotometric Method ◽  
High Sensitivity ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Addition Method ◽  
Ultraviolet Spectrophotometry ◽  
Selective Method ◽  
Good Linear ◽  
Sensitivity And Selectivity

A selective method was developed for the determination of aspirin by Spectrophotometric method .The spectra (range: λ=200~320 nm) were processed. The linear ranges of aspirin was at 50.28 ~ 125.7μg • mL-1 .the aspirin showed good linear correlation.The ultraviolet spectrophotometry data of the samples were also used to evaluate the samples’ quantitative composition.It was shown that recovery of the method by standard addition method was respectively valued 102.06% for aspirin .The method was simple,reliable,accurate,reproducible with high sensitivity and selectivity.

scholarly journals Spectrophotometric method for determination of ranitidine hydrochloride in bulk and in pharmaceutical preparation using ninhydrin

2020 ◽  
Vol 11 (4) ◽  
pp. 291-297
Author(s):  
Hutaf Mustafa Baker ◽  
Hussam Ahmad Alsaoud ◽  
Hamzeh Mohamad Abdel-Halim
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Molar Absorptivity ◽  
Limit Of Quantification ◽  
Ranitidine Hydrochloride ◽  
Relative Standard ◽  
Reproducible Method

A simple, sensitive and reproducible method for the determination of ranitidine hydrochloride in pharmaceutical preparations was investigated. This spectrophotometric method was based on the formation of a deep red color product with ninhydrin in basic media and the absorbance measured at λmax = 480 nm. The reaction occurs at 45 °C with pH = 10 having a contact time of 38 minutes. Under the optimum conditions, Beer’s Law is obeyed in the concentration range of 8.98×103 - 9.90×104 µg/L. The coefficient of correlation was found to be 0.999 for the obtained method with molar absorptivity of 3.05×103 L/mol.cm. The calculated Sandell’s sensitivity is 0.108 μg/cm2. The limit of detection and limit of quantification are 0.0997 and 0.3023 µg/mL, respectively. The low values of the percentage relative standard deviation and percentage relative error indicate the high precision and the good accuracy of the proposed method. The stoichiometry of the reaction is determined and found to be 1:4 (Ranitidine hydrochloride:Ninhydrin). The initial rate method confirmed that this reaction is first order one.

scholarly journals SPECTROPHOTOMETRIC ESTIMATION OF METFORMIN IN HUMAN PLASMA

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Dr Bhavya Sri ◽  
Amina Fatima ◽  
Dr D. Rambabu
Keyword(s):  
Human Plasma ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Standard Addition ◽  
Metformin Hydrochloride ◽  
Plasma Samples ◽  
Addition Method ◽  
Sensitive Spectrophotometric Method

To develop a simple, selective and sensitive spectrophotometric method and validated for the determination of metformin in human plasma. The method was applied for the determination of metformin from pharmaceutical preparations and plasma samples and after spiking with metformin. The results were checked by standard addition method. A number of pharmaceutical additives and plasma matrix did not affect the determination of metformin. The proposed method was found to be accurate and precise for routine estimation of metformin hydrochloride in human plasma. Keywords: Metformin, spectrophotometric, standard addition, plasma, pharmaceutical preparation

Simultaneous spectrophotometric determination of minoxidil and tretinoin by the H-point standard addition method and partial least squares

2009 ◽  
Vol 63 (3) ◽  
Author(s):  
Maryam Bordbar ◽  
Ali Yeganeh-Faal ◽  
Jahanbakhsh Ghasemi ◽  
Mohammad Ahari-Mostafavi ◽  
Nahid Sarlak ◽  
...  
Keyword(s):  
Least Squares ◽  
Partial Least Squares ◽  
Standard Addition ◽  
Partial Least Square ◽  
Least Square ◽  
Standard Addition Method ◽  
Relative Standard Error ◽  
Addition Method ◽  
Relative Standard

AbstractA simple, sensitive and selective spectrophotometric method for simultaneous determination of tretinoin and minoxidil using partial least square (PLS) calibration and H-point standard addition method (HPSAM) is described. The results of the H-point standard addition method show that minoxidil and tretinoin can be determined simultaneously with the concentration ratio of tretinoin to minoxidil varying from 2: 1 to 1: 33 in mixed samples. A partial least squares multivariate calibration method for the analysis of binary mixtures of tretinoin and minoxidil was also developed. The total relative standard error for applying the PLS method to eleven synthetic samples in the concentration range of 0–10 μg mL−1 tretinoin and 0–32 μg mL−1 minoxidil was 2.59 %. Both proposed methods (PLS and HPSAM) were also successfully applied in the determination of tretinoin and minoxidil in several synthetic pharmaceutical solutions.

Spectrophotometric Method for the Assay of Norfloxacin Lactate

2014 ◽  
Vol 989-994 ◽  
pp. 993-996
Author(s):  
Yan Hong Lei
Keyword(s):  
Spectrophotometric Method ◽  
High Sensitivity ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Addition Method ◽  
Selective Method

A selective method was developed for the determination of norfloxacin lactate by Spectrophotometric method. The method was simple with high sensitivity and selectivity.It was shown that recovery of the method by standard addition method was respectively valued 102.05% for norfloxacin lactate

A New Second-Derivative Spectrophotometric Method for the Determination of Vildagliptin in Pharmaceutical dosage form

2021 ◽  
Vol 12 (4) ◽  
pp. 2610-2614
Author(s):  
Subhadip C ◽  
Nalanda Baby R ◽  
Pridhvi Krishna G ◽  
Suraj M ◽  
Shyamdeo Kumar T
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Standard Addition ◽  
Second Derivative ◽  
Limit Of Quantification ◽  
Precision Data ◽  
Pharmaceutical Dosage Form ◽  
Relative Standard

An accurate derivative spectrophotometric method was developed and validated for the determination of dipeptidyl peptidase inhibitor vildagliptin in the pharmaceutical dosage form. The second derivative of the UV spectra has enabled the estimation of vildagliptin absorbance at 217 nm without any interferences. Linearity, precision, accuracy, detection (LOD), and quantification (LOQ) limits were established for method validation. Calibration curve was linear in the range of 10-60 µg/mL with a regression coefficient of 0.998. The method was validated as per the International Conference on Harmonization (ICH Q2 (R1)). The limit of detection and the limit of quantification were found to be 2.06 µg/mL and 6.25 µg/mL, respectively. Intra and interday precision data illustrated that the method has acceptable reproducibility as the percentage relative standard deviation (RSD) was less than 2 %, which indicates the precision of the method. The recovery was 98.39 % by the standard addition method. The percentage assay of vildagliptin was 98.06 % which showed good applicability. The following results indicate that the procedure is accurate, precise, and reproducible while being simple and less time-consuming. The method was demonstrated to be adequate for routine analysis in quality control. 

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