Intraarticular Allogenic Mesenchymal Stem Cells and Vascular Endothelial Growth Factor Injection in Anterior Cruciate Ligament Reconstruction

Graft tunnel healing is important for the successful reconstruction of the anterior cruciate ligament by using the hamstring tendon autograft. There are studies that intra graft tunnel Bone Marrow Mesenchymal stem cells (BMSCs) or intra graft tunnel Vascular endothelial growth factor (VEGF) accelerated graft tunnel healing. This study aimed to investigate the effect of using BMSCs+VEGF injected intra-articular on graft tunnel healing. We reconstructed the anterior cruciate ligament (ACL) of 12 rabbits using an autograft hamstring tendon with and without intra-articular BMSCs+VEGF. Histological evaluation was done at 3 and 6 weeks after ACL reconstruction. On the surface between the graft and the bone tunnel obtained collagen fiber thickness or Sharpey fiber is significantly more than the control group (p< 0.05) in the evaluation of 3 weeks and 6 weeks either side of the tibia and the femur. To evaluate the progression of the treatment, treatment group and control group gained progression had significantly when compared to 3 weeks and 6 weeks. It can be concluded that intra-articular injection of VEGF+BMSCs can accelerate the integration of the graft tunnel from histology evaluation on 3 and 6 weeks.

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Intraarticular Allogenic Mesenchymal Stem Cells and Vascular Endothelial Growth Factor Injection in Anterior Cruciate Ligament Reconstruction

Folia Medica Indonesiana ◽

10.20473/fmi.v56i1.18450 ◽

2020 ◽

Vol 56 (1) ◽

pp. 41

Author(s):

Dwikora Novembri Utomo ◽

Ferdiansyah Mahyudin ◽

Arif Zulkarnain ◽

Purwati Purwati ◽

Rossy Setyawati

Keyword(s):

Stem Cells ◽

Vascular Endothelial Growth Factor ◽

Mesenchymal Stem Cells ◽

Anterior Cruciate Ligament ◽

Cruciate Ligament ◽

Hamstring Tendon ◽

Control Group ◽

Vascular Endothelial ◽

Anterior Cruciate ◽

Endothelial Growth Factor

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Expression of vascular endothelial growth factor and angiogenesis in patellar tendon grafts in the early phase after anterior cruciate ligament reconstruction

Knee Surgery Sports Traumatology Arthroscopy ◽

10.1007/s00167-006-0051-8 ◽

2006 ◽

Vol 14 (9) ◽

pp. 804-810 ◽

Cited By ~ 37

Author(s):

T. Yoshikawa ◽

H. Tohyama ◽

H. Enomoto ◽

H. Matsumoto ◽

Y. Toyama ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Anterior Cruciate Ligament ◽

Growth Factor ◽

Ligament Reconstruction ◽

Cruciate Ligament ◽

Vascular Endothelial ◽

Cruciate Ligament Reconstruction ◽

Tendon Grafts ◽

Anterior Cruciate ◽

Endothelial Growth Factor

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INTRATUNNEL THE EFFECT OF ADMINISTRATION OF BONE MARROW MESENCHYMAL STEM CELLS (BM-MSCs) AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) TENDON-BONE TO INTERFACE HISTOLOGICAL GRAFT ANTERIOR CRUCIATE LIGAMENT APPEARANCE AFTER RECONSTRUCTION IN RABBITS

Journal of Stem Cell Research and Tissue Engineering ◽

10.20473/jscrte.v2i1.9258 ◽

2018 ◽

Vol 2 (1) ◽

Author(s):

Atria Abirama

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Cruciate Ligament ◽

Tendon Graft ◽

Bone Tunnel ◽

Control Group ◽

Vascular Endothelial ◽

Endothelial Growth Factor

The success of the Anterior Cruciate Ligament (ACL) reconstruction using a tendon graft is determined by integration in the bone tendon-graft interface on the bone tunnel. The use of stem cells and growth factors proved to accelerate the healing of the bone tendon-graft interface. The aim of this study was to inveestigate the difference of histology picture in the tendon-bone tunnel model after ACL reconstruction with intratunnel intravenous allogenic bone marrow mesenchymalstemcells (BM-MSCs) and intratunnel vascular endothelial growth factor (VEGF). This research used Post-Test Only Control Group design with 20 rabbits divided into treatment group and control group. Each group performed histologic image evaluation (thickness of collagen fiber or sharpey fiber) at week 3 and 6. Evaluation of histology overview at week 3 and week 6 showed a significantly thicker thickness of collagen fiber or sharpey fiber in treatment group compared with control group (p <0.05). Intravenous administration of BM-SCs and VEGF after ACL reconstruction can speed healing of the bone tunnel significantly from week 3 and 6. The study by Faridyan et al has concluded that intravenous BM-SCs + VEGF increased ultimate tension strength in the bone-tendon interface significantly. In this study, intravenous administration of BM-SCs and VEGF gave histologic images showing acceleration of bone tunnel healing.Keywords:Anterior cruciate ligament reconstruction, allogenic bone marrow mesenchymal stem cells, vascular endothelial growth factor, graft tunnel healing, and Sharpey fiber.

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Effects of Local Administration of Vascular Endothelial Growth Factor on Properties of the in Situ Frozen-Thawed Anterior Cruciate Ligament in Rabbits

The American Journal of Sports Medicine ◽

10.1177/0363546505278700 ◽

2006 ◽

Vol 34 (1) ◽

pp. 84-91 ◽

Cited By ~ 45

Author(s):

Young-Jin Ju ◽

Harukazu Tohyama ◽

Eiji Kondo ◽

Toshikazu Yoshikawa ◽

Takeshi Muneta ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Anterior Cruciate Ligament ◽

Growth Factor ◽

Cruciate Ligament ◽

Local Administration ◽

Vascular Endothelial ◽

Anterior Cruciate ◽

Endothelial Growth Factor

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BIOMECHANIC STUDY OF GRAFT BONE TUNNEL MODEL IN ANTERIOR CRUCIATE LIGAMENT RECONSTRUCTION USING INTRATUNNEL ALLOGENIC BONE MARROW MESENCHYMAL STEM CELLS (BM-MSCs) AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF)

Journal of Stem Cell Research and Tissue Engineering ◽

10.20473/jscrte.v2i1.9262 ◽

2018 ◽

Vol 2 (1) ◽

Author(s):

Brian Vicky Faridyan

Keyword(s):

Vascular Endothelial Growth Factor ◽

Anterior Cruciate Ligament ◽

Cruciate Ligament ◽

Bone Tunnel ◽

Control Group ◽

Vascular Endothelial ◽

Allogenic Bone ◽

Allogenic Bone Marrow ◽

Anterior Cruciate ◽

Tension Strength

Successful anterior cruciate ligament (ACL) reconstruction using tendon graft requires good and rapid integration between the tendon graft and the bone tunnel. The strength of the tendon-bone tunnel graft in the initial phase is very important to facilitate aggressive rehabilitation and as early as possible to support rapid recovery to normal activities. The objective of this study was to determine ultimate tension strength (UTS) on the femoral tendon-bone tunnel graft model after reconstruction of anterior cruciate ligament (ACL) by administering allogenic bone marrow mesenchymal stemcells (BM-MSCs) and vascular endothelial growth factor (VEGF) intratunnel in experimental animals. The design of this research was Post-Test Only Control Group Design using 24 rabbits divided into treatment and control group. Biomechanical evaluation was done at week 3 and 6. Evaluation at week 3 found ultimate tension strength of treatment group significantly higher than control (p <0,05). In the 6th week evaluation, Ultimate tension strength was found that the treatment group significantly higher than the control group (p <0.05). Ultimate tension strength at week 3 did not differ significantly with week 6 (p> 0.05). Intravenous administration of BM-MSCs and VEGF on ACL reconstruction increased ultimate tension strength in graft-bone tunnel significantly since week 3. The study of Ferdiansis et al using BM-MSCs and VEGF intraarticular, only showed a significant increase in ultimate tension strength in graft-bone tunnel since week 6. Comparison of this method indicates acceleration in incorporation of tendon graft with bone tunnel on intratunnel method better thaninvitro intraarticular method.Keywords : Anterior cruciate ligament, allogenic bone marrow mesenchymal stem cells, vascular endothelial growth factor and biomechanic study.

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