scholarly journals Identifikasi Pengaruh Proses Perebusan dan Penggorengan Kacang Tolo (Vigna unguiculata L. Walp.) terhadap Komposisi Fitosterol

2020 ◽  
Vol 7 (1) ◽  
pp. 7
Author(s):  
Noor Erma Nasution Sugijanto ◽  
Citra Hayu Adi Makayasa ◽  
Gita Deseria ◽  
Rahmania Alrika Ilma Bridgeta ◽  
Miftakhul Rohmah Putri ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Vigna Unguiculata ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Flame Ionization Detector ◽  
Ionization Detector ◽  
Mass Selective Detector ◽  
Flame Ionization ◽  
Selective Detector ◽  
Gas Flame

Pendahuluan: Kacang tolo (Vigna unguiculata L. Walp.) merupakan polong-polongan multiguna dapat berfungsi sebagai hipokolesterolemik. Kacang tersebut mengandung protein tinggi dan rendah lemak, sebagai bahan makanan dikonsumsi dengan cara direbus maupun digoreng. Tujuan: Penelitian ini bertujuan untuk mengidentifikasi komposisi fitosterol pada kacang tolo yang mentah, direbus, dan digoreng serta mengidentifikasi pengaruh proses perebusan dan penggorengan kacang tolo terhadap komposisi fitosterol. Metode: Seluruh sampel diperoleh dari Pasar X, Y, dan Z, area Jawa Timur. Masing-masing sampel diekstraksi dengan n-heksana, aseton, dan kloroform kemudian dianalisis dengan Kromatografi Lapis Tipis (KLT), Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR), Kromatografi Gas-Flame Ionization Detector (KG-FID), dan Kromatografi Gas-Mass Selective Detector (KG-MSD). Hasil: Analisis KLT dan ATR-FTIR menunjukkan adanya kandungan fitosterol pada kacang tolo. Analisis dengan KG-FID menunjukkan adanya kolesterol, kampesterol, stigmasterol, dan sitosterol pada kacang tolo mentah dan direbus dari Pasar X, Y, dan Z sedangkan kacang tolo yang digoreng dari Pasar X hanya mengandung stigmasterol dan sitosterol. KG-MSD menunjukkan adanya kolesterol, kampesterol, stigmasterol, dan sitosterol pada kacang tolo mentah, direbus, dan digoreng. Kesimpulan: Kacang tolo mentah, direbus, dan digoreng dapat diidentifikasi adanya ke-empat fitosterol, yaitu kolesterol, kampesterol, stigmasterol, dan β-sitosterol. Proses perebusan dan penggorengan kacang tolo berpengaruh terhadap komposisi fitosterol.

scholarly journals Distinguishing active from quiescent disease in ANCA-associated vasculitis using attenuated total reflection Fourier-transform infrared spectroscopy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adam D. Morris ◽  
Camilo L. M. Morais ◽  
Kássio M. G. Lima ◽  
Daniel L. D. Freitas ◽  
Mark E. Brady ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Unmet Need ◽  
Fourier Transform Infrared ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Remission Induction ◽  
Disease Remission ◽  
Anca Associated Vasculitis ◽  
Active Disease ◽  
Quiescent Disease

AbstractThe current lack of a reliable biomarker of disease activity in anti-neutrophil cytoplasmic autoantibody (ANCA) associated vasculitis poses a significant clinical unmet need when determining relapsing or persisting disease. In this study, we demonstrate for the first time that attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy offers a novel and functional candidate biomarker, distinguishing active from quiescent disease with a high degree of accuracy. Paired blood and urine samples were collected within a single UK centre from patients with active disease, disease remission, disease controls and healthy controls. Three key biofluids were evaluated; plasma, serum and urine, with subsequent chemometric analysis and blind predictive model validation. Spectrochemical interrogation proved plasma to be the most conducive biofluid, with excellent separation between the two categories on PC2 direction (AUC 0.901) and 100% sensitivity (F-score 92.3%) for disease remission and 85.7% specificity (F-score 92.3%) for active disease on blind predictive modelling. This was independent of organ system involvement and current ANCA status, with similar findings observed on comparative analysis following successful remission-induction therapy (AUC > 0.9, 100% sensitivity for disease remission, F-score 75%). This promising technique is clinically translatable and warrants future larger study with longitudinal data, potentially aiding earlier intervention and individualisation of treatment.

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