Identification of Pathogenic Leptospira spp. Carrier Goats  Using Polymerase Chain Reaction (PCR)

Background and Aims: Leptospirosis is a spirochetal disease with public health importance globally. This disease affects a wide range of domestic and wild animals. Dogs are one of the species most sensitive to Leptospira canicola and Leptospira icterohaemorrhagiae. The present study was concluded to evaluate the prevalence rate of Leptospira species and L. canicola and L. icterohaemorrhagiae serovars in Iranian stray dogs. Materials and Methods: One-hundred and twenty blood samples were first taken from stray dogs. Then the samples were transferred to the laboratory. Sera were extracted from blood samples and genomic DNA was extracted. DNA samples were subjected to conventional polymerase chain reaction. Positive samples for Leptospira spp. were analyzed for presence of L. canicola and L. icterohaemorrhagiaeserovars using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: Nine samples out of 120 serum samples (7.5%) were positive for the flagella gene of the Leptospira spp. Prevalence of Leptospira spp. in serum samples of male and female dogs were 5.4% and 10.86%, respectively. Prevalence of L. canicola and L. icterohaemorrhagiae serovars were 55.55% and 33.33%, respectively. We found that 11.11% of samples were positive for both serovars. Two to three and 3-4 year old dogs had the highest prevalence of Leptospira spp. Conclusions: The considerable prevalence of leptospirta spp. and also their zoonotic serovars among Iranian stray dogs represented an important public health issue regarding the contact of healthy human with these dogs. Identification of infected dogs and their vaccination can inhibit the distribution of Leptospira spp.

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Development and validation of real time polymerase chain reaction for detection DNA of pathogenic leptospira

Bulletin "Veterinary biotechnology" ◽

10.31073/vet_biotech32(2)-69 ◽

2018 ◽

Vol 32 (2) ◽

pp. 565-576

Author(s):

V. V. Ukhovskyi ◽

L. M. Muzykina ◽

I. V. Galka ◽

V. G. Spyrydonov ◽

I. I. Antonik ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Chain Reaction ◽

Pathogenic Leptospira ◽

Polymerase Chain ◽

Development And Validation

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Detection of Leptospira spp. using polymerase chain reaction technique from kidney of Rattus norvegicus from Grenada, West Indies

International Journal of One Health ◽

10.14202/ijoh.2019.81-85 ◽

2019 ◽

pp. 81-85

Author(s):

Bhumika Sharma ◽

Katelyn Thille ◽

Nia Rametta ◽

Ravindra Sharma

Keyword(s):

Polymerase Chain Reaction ◽

Rattus Norvegicus ◽

West Indies ◽

Gene Segment ◽

Local Alignment ◽

Active Infection ◽

Chain Reaction ◽

Significant Difference ◽

Leptospira Spp ◽

Polymerase Chain

Aim: This study aimed to find out the prevalence of active infection of Leptospira spp. in Rattus norvegicus from Grenada, West Indies, through polymerase chain reaction (PCR). Materials and Methods: One hundred and forty-nine rats were trapped, anesthetized and their kidneys collected aseptically. DNA was extracted from the kidney tissue of each rat. PCR was performed targeting LipL32 gene. Eighteen PCR-positive amplicons for LipL32 gene segment were purified and sent for direct sequencing to the sequencing facility of MCLAB (South San Francisco, USA). Results of sequencing were read and interpreted. The prevalence of Leptospira spp. in relation to sex and age was also recorded. Results: All amplified sequences were compared to the sequences present in GenBank using basic local alignment search tool (BLAST) from the online website National Center for Biotechnology Information, the results revealed that six samples had similarity to Leptospira interrogans strain 1399/2016 and eight samples had similarity with Leptospira borgpetersenii serovar Hardjo-bovis strain L49. Of 149 kidney samples, only 14 were positive for Leptospira spp. by PCR giving an incidence of 9.3%. There was no significant difference found in relation to sex and age. Conclusion: This is the first report confirming active infection of Leptospira spp. in Rattus norvegicus in Grenada using PCR. The presence of active infection in rats can be considered as high risk for humans. Further research to understand the epidemiology of leptospirosis in Grenada is suggested.

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Leptospira Detection in Cats in Spain by Serology and Molecular Techniques

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17051600 ◽

2020 ◽

Vol 17 (5) ◽

pp. 1600 ◽

Cited By ~ 1

Author(s):

Andrea Murillo ◽

Rafaela Cuenca ◽

Emmanuel Serrano ◽

Goris Marga ◽

Ahmed Ahmed ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Human Infection ◽

Molecular Techniques ◽

Antibody Prevalence ◽

Chain Reaction ◽

Pathogenic Leptospira ◽

Blood Samples ◽

Polymerase Chain ◽

Positive Results ◽

Leptospira Species

Leptospirosis is the most neglected widespread zoonosis worldwide. In Spain, leptospirosis reports in people and animals have increased lately. Cats can become infected with Leptospira, as well as be chronic carriers. The aim of this study was to determine serological antibody prevalence against Leptospira sp., blood DNA, and shedding of DNA from pathogenic Leptospira species in the urine of cats in Spain. Microagglutination tests (MAT) and blood and urine TaqMan real-time polymerase chain reaction (PCR) were performed. Leptospira antibodies were detected in 10/244 cats; with 4.1% positive results (95% confidence interval (CI): 2.1–7.18%). Titers ranged from 1:20 to 1:320 (serovars Ballum; Bataviae; Bratislava; Cynopteri; Grippotyphosa Mandemakers; Grippotyphosa Moskva; Pomona; and Proechimys). The most common serovar was Cynopteri. Blood samples from 1/89 cats amplified for Leptospira DNA (1.12%; 95% CI: 0.05–5.41%). Urine samples from 4/232 cats amplified for Leptospira DNA (1.72%; 95% CI: 0.55–4.10%). In conclusion free-roaming cats in Spain can shed pathogenic Leptospira DNA in their urine and may be a source of human infection. Serovars not previously described in cats in Spain were detected; suggesting the presence of at least 4 different species of pathogenic leptospires in the country (L. borgpetersenii; L. interrogans; L. kirschneri; and L. noguchii).

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SEROLOGICAL AND MOLECULAR SURVEY OF Leptospira spp. AMONG CART HORSES FROM AN ENDEMIC AREA OF HUMAN LEPTOSPIROSIS IN CURITIBA, SOUTHERN BRAZIL

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652014000600003 ◽

2014 ◽

Vol 56 (6) ◽

pp. 473-476 ◽

Cited By ~ 8

Author(s):

Mariane Angélica Finger ◽

Ivan Roque de Barros Filho ◽

Christian Leutenegger ◽

Marko Estrada ◽

Leila Sabrina Ullmann ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Endemic Area ◽

Agglutination Test ◽

Southern Brazil ◽

Chain Reaction ◽

Human Leptospirosis ◽

Leptospira Spp ◽

Polymerase Chain ◽

Positive Correlations

Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.

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