scholarly journals Effective Surface Sterilization Method using Plant Preservatives Mixture and Shoot Multiplication of Clinacanthus nutans

2020 ◽  
Vol 9 (3) ◽  
pp. 3240-3251
Author(s):  
Florisa Landa ◽  
Yvonne C. Chia ◽  
Roslin Ombokou ◽  
Zaleha A. Aziz
Keyword(s):  
Shoot Multiplication ◽  
Effective Surface ◽  
Surface Sterilization ◽  
Sterilization Method ◽  
Clinacanthus Nutans

scholarly journals Optimization of Seed Surface Sterilization Method and in vitro Seed Germination in Althaea officinalis (L.) - An Important Medicinal Herb

2016 ◽  
Vol 9 (28) ◽  
Author(s):  
Fatemeh Soghra Younesikelaki ◽  
Mohammad Hadi Ebrahimzadeh ◽  
Mehdi Kiani Desfardi ◽  
Mahitha Banala ◽  
Rajinikanth Marka ◽  
...  
Keyword(s):  
Seed Germination ◽  
Medicinal Herb ◽  
Surface Sterilization ◽  
Seed Surface ◽  
Sterilization Method ◽  
Althaea Officinalis

scholarly journals In Vitro Propagation of Viburnum treleasei Gand., an Azorean Endemic with High Ornamental Interest

HortScience ◽  
2009 ◽  
Vol 44 (6) ◽  
pp. 1668-1671 ◽  
Author(s):  
Mónica Moura ◽  
Maria Irene Candeias ◽  
Luís Silva
Keyword(s):  
In Vitro Propagation ◽  
Natural Populations ◽  
Shoot Multiplication ◽  
Shoot Development ◽  
Naphthaleneacetic Acid ◽  
Benzyl Adenine ◽  
Single Node ◽  
Surface Sterilization ◽  
Rare And Endangered

The purpose of our research was to establish a protocol for the in vitro culture of Viburnum treleasei, a rare and endangered taxon with high ornamental potential endemic to the Azores islands. The surface sterilization of the explants was better achieved with a pretreatment of 0.1% (w/v) Benomyl for 2 h followed by 0.2% (w/v) HgCl2 for 10 min with agitation. Shoot tips were the most efficient explants for shoot development and single-node segments for proliferation. Woody plant medium (WPM) was adequate for all micropropagation stages. For culture establishment and shoot development, a hormone-free medium was adequate, whereas a 1.1 μM N6-benzyl adenine medium supplement was more efficient for shoot multiplication. Elongation and rooting could be carried out on a 1.3 μM 1-naphthaleneacetic acid-supplemented medium. Acclimatization of in vitro-produced plantlets was achieved after 1 month with a success rate of 50%. This in vitro propagation procedure will be useful for the conservation of Viburnum treleasei through production of morphologically true-to-type plants, allowing the recovery of depleted natural populations. Chemical names used: N6-benzyl adenine (BA); 1-naphthaleneacetic acid (NAA); HgCl2 (mercury bichloride).

SEED STERILIZATION USING SILVER IONS ANDSILVER NANOPARTICES SYNTHESIZED BY GREEN CHEMISTRY METHODS

2020 ◽  
pp. 343-344
Author(s):  
A. Popova ◽  
V. Zaitsev
Keyword(s):  
Silver Nanoparticles ◽  
Green Chemistry ◽  
Germination Rate ◽  
Silver Ions ◽  
Effective Surface ◽  
Surface Sterilization ◽  
High Germination

Most effective surface sterilization of barley seeds is treatment by 0,14% AgNO3, producing maximal sterilization level coupled with high germination rate. Treatments of the seeds by H2O2 or silver nanoparticles synthesized by green chemistry methods were inefficient.

A New RoVe Method to Purify Endophytic Fungi from Medicinal Plants Western Ghats of Karnataka India

2020 ◽  
Vol 13 (4) ◽  
pp. 5011-5019
Author(s):  
Ankala Basappa Vedamurthy ◽  
Rohit Shankar Mane
Keyword(s):  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Aloe Vera ◽  
Thielaviopsis Basicola ◽  
Surface Sterilization ◽  
Epiphytic Microorganisms ◽  
Sterilization Method ◽  
Successful Isolation ◽  
Aspergillus Nomius

Endophytic fungi from medicinal plants were hardly ever reported as compared to those from soil and marine sources. The present results associate the presence of endophytic fungi in medicinal plants by isolating them from three medicinal plants i.e. Bombax ceiba, Aloe vera, and Ximenia americana. In the present research investigation, surface sterilization method and media were standardized, and 32 endophytic fungi were isolated from three medicinal plants. We assessed the competence of three different surface sterilization methods and four media for isolation of endophytic fungi. The RoVe method used was more effective in eliminating epiphytic microorganisms. Therefore by using new method we have isolated total of 32 fungal endophytes and those were belongs to Aspergillus nomius (63.20%), Aspergillus niger (41.60%), Thielaviopsis basicola (38.33%), Fusarium oxysporum (33.20%), Pestalotiopsis inflexa (27.20%), Nigrospora sphaerica (20%), Alternaria alternata (15.30%) and Phomopsis archeri (20%). This is the first report of successful isolation of endophytic fungi from the said medicinal plants, for using newly formulated surface sterilization method.

scholarly journals Optimization of in vitro asymbiotic seed germination protocol for Serapias vomeracea

2019 ◽  
Vol 3 (3) ◽  
pp. 143-151 ◽  
Author(s):  
Arda Acemi ◽  
Fazıl Özen
Keyword(s):  
Seed Germination ◽  
Liquid Form ◽  
Tetrazolium Chloride ◽  
Asymbiotic Seed Germination ◽  
Surface Sterilization ◽  
Seed Surface ◽  
Sterilization Method ◽  
Germination Success ◽  
The Media

Abstract Serapias vomeracea is an economically important orchid species which is over-collected from nature, because of its glucomannan-rich tubers. Thus, optimization of in vitro culture methodology in this species is required to meet industrial needs and to secure its populations in nature. This study aimed to optimize the surface sterilization protocol for S. vomeracea seeds and to select the optimal seed germination medium by comparing the commonly used media in in vitro orchid culture. During seed surface sterilization, ethyl alcohol (EtOH) pre-treatment prior to sodium hypochlorite (NaOCl) treatment increased the disinfection success and viable seed yield when examined using the triphenyl tetrazolium chloride (TTC) seed viability test. Also, low-g force centrifugation as an additional step in the surface sterilization method separated the seeds without embryo from the viable seeds and thereby decreased potential counting errors after incubation. Comparison of media showed that solid Knudson C (KN) medium induced the highest number of germinated seeds. However, seed germination success of Lindemann (LN) and Vacin & Went (VW) media was found to be higher when the media was used in liquid form. Half-strength liquid VW was the only medium that induced higher germination success than the other full-strength media. The highest number of ungerminated seeds was found when using KN medium whereas liquid VW medium gave the lowest number. In general, protocorm formation was triggered when the media were used in liquid form. However, rhizoid elongation was suppressed in liquid media. These findings suggest that this optimized seed surface sterilization method offers a simple and effective alternative to classical methods. Additionally, solid KN medium may be considered as a cost-effective and reliable alternative to other commonly-used complex media in S. vomeracea cultures.

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