EFEK LAMA WAKTU PEMBERIAN EKSTRAK ETANOL SELEDRI (Apium graveolens L.) TERHADAP PENCEGAHAN PENINGKATAN KADAR IL-1β PADA TIKUS MODEL ISCHEMIA-REPERFUSION INJURY

Ischemia-reperfusion injury (IRI) is the condition that disrupted the blood supply to the organ followed by the restoration of blood flow. IRI in the kidneys promotes the inflammatory cascade. Interleukin-1β (IL-1β) belongs to the most powerful pro-inflammatory cytokine that released in the early phase of IRI. Prevention of inflammation is one of the strategies for reducing kidney damage due to IRI. Celery (Apium graveolens L) is a natural resource that reported has anti-inflammatory and antioxidant effects. This study aimed to investigate the effect of duration of the administration ethanol extract of celery on IL-1β level in the IRI rat model. Twenty-five rats male Sprague Dawley, 2-3 months old were divided into 5 groups: Sham operations (SO, n=5), Ischemia-reperfusion (IR, n=5), celery 1000 mg/ kg BW for 7 days before IR (IR7) (IR7, n=5), 14 days before IR (IR14, n=5), 28 days before IR (IR28, n=5). The IL-1β level was assessed using the ELISA. Data were analyzed using One-way ANOVA (p<0.05). The results showed that the mean of IL-1β levels in the IR7 group (5.99±4.28 ng/L) and R14 (4.68±2.64 ng/L) were lower than IR group (8.19±5.36 ng/L), while the R28 group (9.05 ± 4.38 ng/L) was higher than the IR group (8.19±5.36 ng/L). In conclusion, the administration of celery ethanol extract 1000 mg/ kg BW for 7 days and 14 days can prevent the increase of IL-1β level in the IRI rat model. Ischemia-reperfusion injury (IRI) adalah suatu keadaan yang terjadi ketika suplai darah sebagian atau seluruh organ terganggu diikuti pemulihan aliran darah. IRI pada ginjal memicu terjadinya inflamasi. Interleukin-1β (IL-1β) termasuk sitokin pro-inflamasi paling kuat yang keluar selama fase awal IRI. Pencegahan terhadap inflamasi merupakan strategi untuk menurunkan kerusakan ginjal akibat IRI. Seledri merupakan salah satu bahan alam yang diketahui mempunyai efek antiiflamasi dan antioksidan. Penelitian ini bertujuan mengetahui efek lama waktu pemberian ekstrak etanol seledri terhadap pencegahan peningkatan kadar IL-1β pada tikus model IRI. Sebanyak 25 ekor tikus jantan Sprague Dawley, 2-3 bulan, dikelompokkan menjadi 5, yaitu Sham Operation (SO, n=5), Ischemia-reperfusion (IR, n=5), seledri 1000 mg/kgBB selama 7 hari sebelum IR(IR7, n=5), 14 hari sebelum IR(IR14, n=5), dan 28 hari sebelum IR(IR28, n=5). Kadar IL-1β diukur menggunakan metode ELISA. Data dianalisis dengan One Way ANOVA (p<0,05). Rerata kadar IL-1β pada kelompok IR7 (5,99±4,28 ng/L) dan IR14 (4,68±2,64 ng/L) lebih rendah dibandingkan dengan kelompok IR (8,19±5,36 ng/L), sedangkan kelompok IR28 (9,05±4,38 ng/L) lebih tinggi dibandingkan dengan kelompok IR (8,19±5,36 ng/L). Pemberian ekstrak etanol seledri 1000 mg/kgBB selama 7 hari dan 14 hari dapat mencegah peningkatan kadar IL-1β pada tikus model IRI.

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Protective Effect of Ethanol Extract of Celery (Apium graveolens L) on Kidney Damage in Ischemia/ Reperfusion Injury Rats Model

Molekul ◽

10.20884/1.jm.2019.14.1.448 ◽

2019 ◽

Vol 14 (1) ◽

pp. 11

Author(s):

Afifah Afifah ◽

Khusnul Muflikhah ◽

Viva Ratih Bening Ati ◽

Rizki Maulana Tsani ◽

Dairotul Khasanah ◽

...

Keyword(s):

Protective Effect ◽

Reperfusion Injury ◽

Rat Model ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Ethanol Extract ◽

Kidney Damage ◽

Apium Graveolens ◽

Tubular Injury ◽

Injury Score

Ischemia/ reperfusion injury (IRI) is marked by the sudden decrease of blood supply to the kidney followed by restoration of blood flow. Ischemic acute kidney injury (AKI) is the leading cause of morbidity and mortality. Therefore, the prevention strategy for decreasing the damage due to IRI is required. Celery (Apium graveolens L) is often consumed as food. Celery has antioxidant and anti-inflammatory effects. This study aimed to investigate the protective effect of the celery on kidney damage in the kidney ischemia/ reperfusion injury rat model. Twenty-five rats male, 2-3 months old Sprague Dawley were divided into 5 groups: Group 1 was sham operation, group 2 was ischemia/ reperfusion injury (IRI), group 3, 4, 5 were IRI and 250 mg/kgBW, 500 mg/kgBW, 1000 mg/kgBW of ethanol extract of celery respectively for 14 days before and 3 days after operation. Blood serum sample was taken 3 days after the operation for measuring urea, creatinine, superoxide dismuthase (SOD) and nitrite oxide (NO). Hematoxylin-eosin (HE) staining was utilized to examine kidney tubular injury score. Data were analyzed using One-way ANOVA (p<0,05). The ethanol extract of celery at dose 1000 mg/kgBW prevented the increase of urea, creatinine serum, kidney tubular injury score and prevented the decrease of SOD, NO in the kidney ischemia/ reperfusion injury rat model (p<0,05). In conclusion, the ethanol extract of celery has a protective effect on kidney damage in the ischemia/ reperfusion injury rat model.

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Evaluation of the Antioxidant Potential of Salvia miltiorrhiza Ethanol Extract in a Rat Model of Ischemia-Reperfusion Injury

Molecules ◽

10.3390/molecules161210002 ◽

2011 ◽

Vol 16 (12) ◽

pp. 10002-10012 ◽

Cited By ~ 38

Author(s):

Zengyong Qiao ◽

Jiangwei Ma ◽

Huajin Liu

Keyword(s):

Reperfusion Injury ◽

Rat Model ◽

Ischemia Reperfusion Injury ◽

Salvia Miltiorrhiza ◽

Ischemia Reperfusion ◽

Ethanol Extract ◽

Antioxidant Potential

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Melatonin and Glycine Reduce Uterus Ischemia/Reperfusion Injury in a Rat Model of Warm Ischemia

International Journal of Molecular Sciences ◽

10.3390/ijms22168373 ◽

2021 ◽

Vol 22 (16) ◽

pp. 8373

Author(s):

Viktorija Zitkute ◽

Mindaugas Kvietkauskas ◽

Vygante Maskoliunaite ◽

Bettina Leber ◽

Diana Ramasauskaite ◽

...

Keyword(s):

Reperfusion Injury ◽

Rat Model ◽

Ischemia Reperfusion Injury ◽

Cell Damage ◽

Ischemia Reperfusion ◽

Combined Treatment ◽

Warm Ischemia ◽

Control Group ◽

Protective Effects ◽

Sprague Dawley

Ischemia/reperfusion injury (IRI) remains a significant problem to be solved in uterus transplantation (UTx). Melatonin and glycine have been shown to possess direct cytoprotective activities, mainly due to their antioxidative and anti-inflammatory properties. The aim of this study was to investigate the protective effects of melatonin and glycine and their combination on IRI in a rat model of warm ischemia. In this study, Sprague-Dawley rats were assigned to eight groups, including sham and IRI (n = 80). Melatonin and glycine alone or their combination were administered prior to 1 h of uterus ischemia followed by 1 h of reperfusion. Melatonin (50 mg/kg) was administered via gavage 2 h before IRI and glycine in an enriched diet for 5 days prior to intervention. Uterus IRI was estimated by histology, including immunohistochemistry, and biochemical tissue analyses. Histology revealed that uterus IRI was significantly attenuated by pretreatment with melatonin (p = 0.019) and glycine (p = 0.044) alone as well as their combination (p = 0.003). Uterus IRI led to increased myeloperoxidase expression, which was significantly reduced by melatonin (p = 0.004), glycine (p < 0.001) or their combination (p < 0.001). The decline in superoxide dismutase activity was significantly reduced in the melatonin (p = 0.027), glycine (p = 0.038) and combined treatment groups (p = 0.015) when compared to the IRI control group. In conclusion, melatonin, glycine and their combination significantly reduced oxidative stress-induced cell damage after IRI in a small animal warm ischemia model, and, therefore, clinical studies are required to evaluate the protective effects of these well-characterized substances in uterus IRI.

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