scholarly journals Research of pigments of blue-green algae spirulina platensis for practical use in confectionery technology

Author(s):  
T. K. Kalenik ◽  
E. V. Dobrynina ◽  
V. M. Ostapenko ◽  
Y. Torii ◽  
J. Hiromi

The article presents a study of the process of isolation of natural blue pigment – phycocyanin from the biomass of blue-green algae Spirulina platensis by water extraction, followed using its water solution as a natural food colorant in the production of milk chocolate. Recently, modern food enterprises are pursuing their policy towards expanding the range of products, which is closely related to the increasing needs of the population in food of a new kind. One of the solutions to this problem is the use of food additives of both natural and synthetic origin. Among the similar components widespread found dyes synthetic origin, which have high coverage rates and relatively low cost. However, many of the permitted in our country synthetic food dyes are banned in several developed countries as potentially dangerous to health. Synthetic dyes of red, yellow and green color have many natural analogues – carotenoids, lutein, chlorophyll, etc., except for the blue dye, the analogue of which is only anthocyanins, which are unstable depending on the pH conditions. In this article were identified phycobiliproteins and chlorophyll a in a water extract of spirulina. The mass concentration of phycobiliproteins and chlorophyll a was determined by spectrophotometric method before and after the addition of ammonium sulfate. A comparative analysis of the effect of fractionation (salting out) on the degree of purification of the phycocyanin solution. Presented and described the technological scheme of extraction of phycocyanin which allows to use it in food technologies as an extract or a dry powder. Established the concentration of phycocyanin extract from blue-green algae spirulina to produce milk blue chocolate. Determined organoleptic and hygienic characteristics of the finished product

1992 ◽  
Vol 49 (11) ◽  
pp. 2331-2336 ◽  
Author(s):  
D. J. Webb ◽  
B. K. Burnison ◽  
A. M. Trimbee ◽  
E. E. Prepas

Chlorophyll a (Chl a) in water samples from three mesotrophic to eutrophic lakes in north-central Alberta was extracted with one of three solvents (95% ethanol, 90% ethanol, or a 2:3 mixture of dimethyl sulfoxide and 90% acetone (DMSO/acetone)) and analyzed by two techniques (spectrophotometry and high pressure liquid chromatography (HPLC). The dominant phytoplankton were blue-green algae and diatoms. Total Chl a concentrations (i.e. no correction for phaeopigments (Pha)) were not significantly different among solvents (P > 0.5). Total Chl a concentrations from spectrophotometric analyses were significantly higher than those from HPLC analyses (4.2 ± 0.88 and 2.6 ± 0.50 μg∙L−1 respectively, P < 0.05). Pha concentrations derived by spectrophotometry were 64 times higher than those derived by HPLC (1.7 ± 0.52 and 0.025 ± 0.01 μg∙L−1 respectively, P < 0.005). Thus, spectrophotometry appears to dramatically overestimate Pha concentrations and may overestimate total Chl a (i.e. no correction for Pha). Therefore, ethanol and DMSO/acetone are equally suitable for Chl a extraction from natural populations dominated by blue-green algae and/or diatoms, but if information on Pha and/or accessory pigments is required, HPLC analyses are the appropriate route rather than spectrophotometry.


2019 ◽  
Vol 1 (2) ◽  
pp. 37
Author(s):  
Katarina Purnomo Salim ◽  
Rosita Dwi Chandra ◽  
Heriyanto Heriyanto ◽  
Dwi Susilaningsih ◽  
Leenawaty Limantara ◽  
...  

Current efforts to explore the potency of marine resources have been made to promote economic development in Indonesia, especially in the industrial sector. Marine cyanobacteria such as Arthospira sp. and Scytonema sp. are two potential candidates of the blue-green algae that can be used in the application for food and health industries. This study focused on the identification of the composition of the dominant chlorophylls and carotenoids. The results showed the presence of Zeaxanthin, Chlorophyll a, and β-carotene. The economic potency of those dominant pigments will be reviewed in this article.


Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1219
Author(s):  
Elaheh Madadi ◽  
Sahand Mazloum-Ravasan ◽  
Jae Sik Yu ◽  
Ji Won Ha ◽  
Hamed Hamishehkar ◽  
...  

Anthocyanins, betalains, riboflavin, carotenoids, chlorophylls and caramel are the basic natural food colorants used in modern food manufacture. Betalains, which are composed of red–violet betacyanin and yellow betaxanthins, are water-soluble pigments that color flowers and fruits. Betalains are pigments primarily produced by plants of the order Caryophyllales. Because of their anti-inflammatory, cognitive impairment, anticancer and anti-hepatitis properties, betalains are useful as pharmaceutical agents and dietary supplements. Betalains also exhibit antimicrobial and antimalarial effects, and as an example, betalain-rich Amaranthus spinosus displays prominent antimalarial activity. Studies also confirmed the antidiabetic effect of betalains, which reduced glycemia by 40% without causing weight loss or liver impairment. These findings show that betalain colorants may be a promising alternative to the synthetic dyes currently used as food additives.


1984 ◽  
Vol 41 (10) ◽  
pp. 1519-1522 ◽  
Author(s):  
Barbara J. Speziale ◽  
Stephen P. Schreiner ◽  
Paul A. Giammatteo ◽  
James E. Schindler

We evaluated the suitability of N,N-dimethylformamide (DMF) for extraction and measurement of chlorophyll in phytoplankton and compared the extraction efficiencies of DMF, dimethyl sulfoxide (DMSO)–acetone, and 90% acetone. Absorbance spectra and specific absorption coefficients of chlorophylls a, b, and c in DMF and 90% acetone are similar. Acidification of 100% DMF extracts of pure chlorophyll a and healthy algal cultures produced acid ratios approximating 1.7. Thus, equations derived from the extinction coefficients of chlorophylls in 90% acetone can be applied to DMF extracts. DMF and DMSO–acetone each extract chlorophyll from green and blue-green algae more effectively than does 90% acetone. DMF is a better extractant than is DMSO–acetone for chlorococcalean species, especially when pigment concentrations are high, but the difference, although significant, is slight. The three solvents are equally efficient in extracting chlorophyll from samples composed of chrysophytes and flagellated chlorophytes. The lack of grinding when using either DMF or DMSO–acetone is a considerable convenience. The potential for destabilized spectrophotometer readings and contamination of the investigator due to the incomplete mixing of DMSO and acetone is eliminated when pure DMF is used.


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