scholarly journals Lipid Membrane Based Biosensors

2018 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Dimitrios Nikolelis ◽  
Christina Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Food Quality ◽  
Lipid Membranes ◽  
Recent Progress ◽  
Lipid Membrane ◽  
Environmental Pollutants ◽  
Quality Monitoring ◽  
Future Prospects ◽  
Considerable Part ◽  
Bilayer Device ◽  
Food Toxicants

The exploitation of lipid membranes in biosensors has provided the ability to reconstitute a considerable part of their functionality to detect trace of food toxicants and environmental pollutants. Nanotechnology enabled sensor miniaturization and extended the range of biological moieties that could be immobilized within a lipid bilayer device. This chapter reviews recent progress in biosensor technologies based on lipid membranes suitable for environmental applications and food quality monitoring. Numerous biosensing applications are presented, putting emphasis on novel systems, new sensing techniques and nanotechnology-based transduction schemes. The range of analytes that can be currently detected include, insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc. Technology limitations and future prospects are discussed, focused on the evaluation/ validation and eventually commercialization of the proposed sensors.

scholarly journals Lipid Membrane Based Biosensors

2018 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Dimitrios P. Nikolelis ◽  
Christina G. Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Food Quality ◽  
Lipid Membranes ◽  
Recent Progress ◽  
Lipid Membrane ◽  
Environmental Pollutants ◽  
Quality Monitoring ◽  
Future Prospects ◽  
Considerable Part ◽  
Bilayer Device ◽  
Food Toxicants

The exploitation of lipid membranes in biosensors has provided the ability to reconstitute a considerable part of their functionality to detect trace of food toxicants and environmental pollutants. Nanotechnology enabled sensor miniaturization and extended the range of biological moieties that could be immobilized within a lipid bilayer device. This chapter reviews recent progress in biosensor technologies based on lipid membranes suitable for environmental applications and food quality monitoring. Numerous biosensing applications are presented, putting emphasis on novel systems, new sensing techniques and nanotechnology-based transduction schemes. The range of analytes that can be currently detected include, insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc. Technology limitations and future prospects are discussed, focused on the evaluation/ validation and eventually commercialization of the proposed sensors.

scholarly journals The Application of Lipid Membranes in Biosensing

Membranes ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 108 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Dimitrios Nikolelis ◽  
Christina Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Food Quality ◽  
Lipid Membranes ◽  
Recent Progress ◽  
Lipid Membrane ◽  
Environmental Pollutants ◽  
Quality Monitoring ◽  
Future Prospects ◽  
Considerable Part ◽  
Lipid Film ◽  
Food Toxicants

The exploitation of lipid membranes in biosensors has provided the ability to reconstitute a considerable part of their functionality to detect trace of food toxicants and environmental pollutants. This paper reviews recent progress in biosensor technologies based on lipid membranes suitable for food quality monitoring and environmental applications. Numerous biosensing applications based on lipid membrane biosensors are presented, putting emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The range of analytes that can be currently using these lipid film devices that can be detected include, insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc. Technology limitations and future prospects are discussed, focused on the evaluation/validation and eventually commercialization of the proposed lipid membrane-based biosensors.

Applications of Lipid Membranes-based Biosensors for the Rapid Detection of Food Toxicants and Environmental Pollutants

2019 ◽  
pp. 285-297
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Dimitrios P. Nikolelis ◽  
Christina G. Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Lipid Membranes ◽  
Rapid Detection ◽  
Environmental Pollutants ◽  
Food Toxicants

scholarly journals Recent Lipid Membrane-Based Biosensing Platforms

2019 ◽  
Vol 9 (9) ◽  
pp. 1745 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Christina G. Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Spyridoula Bratakou ◽  
Dimitrios K. Bendos
Keyword(s):  
Lipid Membrane ◽  
Clinical Chemistry ◽  
Environmental Pollutants ◽  
Membrane Technology ◽  
Lipid Film ◽  
Wide Range ◽  
Lipid Films ◽  
Clinical Interest ◽  
Food Toxicants

The investigation of lipid films for the construction of biosensors has recently given the opportunity to manufacture devices to selectively detect a wide range of food toxicants, environmental pollutants, and compounds of clinical interest. Biosensor miniaturization using nanotechnological tools has provided novel routes to immobilize various “receptors” within the lipid film. This chapter reviews and exploits platforms in biosensors based on lipid membrane technology that are used in food, environmental, and clinical chemistry to detect various toxicants. Examples of applications are described with an emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The compounds that can be monitored are insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc.

scholarly journals Nanotechnological advances of Lipid film-based biosensors for the rapid detection of biological compounds and toxicants

2019 ◽  
pp. 32-40
Author(s):  
Georgia-Paraskevi Nikoleli
Keyword(s):  
Lipid Membranes ◽  
Lipid Membrane ◽  
Cholesterol Oxidase ◽  
Environmental Pollutants ◽  
Clinical Analysis ◽  
Lipid Film ◽  
Biological Interest ◽  
Wide Range ◽  
Biological Compounds ◽  
Novel Devices

The exploration of lipid membranes for the construction of nanobiosensors has recently provided the opportunity to construct devices to monitor a wide range of compounds of biological interest. Nanobiosensor miniaturization using nanotechnological tools has given novel ways to attach a wide range of “receptors” in the lipid membrane. The lipids used to construct a lipid film-based device are dipalmiloylphosphatidylcholine {DPPC} and in some cases dipalmitoylphosphatidic acid (DPPA) which is an anionic lipid and is used to increase the sensitivity of detection. Most common “receptors” used in lipid film biosensors are enzymes such as urease, cholesterol oxidase, urecase, etc, antibodies such as D-dimer antibody and artificial or natural receptors such as saxitoxin, cholera toxin, calyx [4] arene phospjoryl receptor, etc. This chapter reviews and investigates the construction of nanobiosensors based on lipid membranes that are used to monitor various toxicants. It also exploits examples of applications with an emphasis on novel devices, new nanobiosensing techniques and nanotechnology-based transduction schemes. The compounds that can be detected are insecticides, toxins, hormones, dioxins, etc. Keywords: Lipid membrane based nanosensors; Nanoyechology; Graphene and ZnO electrodes; Food toxicants; Environmental pollutants; Clinical analysis

scholarly journals Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles

Membranes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Damian Dziubak ◽  
Kamil Strzelak ◽  
Slawomir Sek
Keyword(s):  
Electrochemical Properties ◽  
Self Assembly ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Membrane Resistance ◽  
Electrochemical Characteristics ◽  
Freeze Thaw ◽  
Lipid Films ◽  
Supported Lipid Membranes

Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance.

scholarly journals Interactions of Linear Analogues of Battacin with Negatively Charged Lipid Membranes

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 192
Author(s):  
Kinga Burdach ◽  
Dagmara Tymecka ◽  
Aneta Urban ◽  
Robert Lasek ◽  
Dariusz Bartosik ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Liquid Crystalline ◽  
Lipid Membrane ◽  
Attenuated Total Reflection ◽  
Gram Positive ◽  
Insertion Depth ◽  
Force Microscopy ◽  
Acyl Chains ◽  
Hydrophobic Portion ◽  
Membrane Fluidization

The increasing resistance of bacteria to available antibiotics has stimulated the search for new antimicrobial compounds with less specific mechanisms of action. These include the ability to disrupt the structure of the cell membrane, which in turn leads to its damage. In this context, amphiphilic lipopeptides belong to the class of the compounds which may fulfill this requirement. In this paper, we describe two linear analogues of battacin with modified acyl chains to tune the balance between the hydrophilic and hydrophobic portion of lipopeptides. We demonstrate that both compounds display antimicrobial activity with the lowest values of minimum inhibitory concentrations found for Gram-positive pathogens. Therefore, their mechanism of action was evaluated on a molecular level using model lipid films mimicking the membrane of Gram-positive bacteria. The surface pressure measurements revealed that both lipopeptides show ability to bind and incorporate into the lipid monolayers, resulting in decreased ordering of lipids and membrane fluidization. Atomic force microscopy (AFM) imaging demonstrated that the exposure of the model bilayers to lipopeptides leads to a transition from the ordered gel phase to disordered liquid crystalline phase. This observation was confirmed by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) results, which revealed that lipopeptide action causes a substantial increase in the average tilt angle of lipid acyl chains with respect to the surface normal to compensate for lipopeptide insertion into the membrane. Moreover, the peptide moieties in both molecules do not adopt any well-defined secondary structure upon binding with the lipid membrane. It was also observed that a small difference in the structure of a lipophilic chain, altering the balance between hydrophobic and hydrophilic portion of the molecules, results in different insertion depth of the active compounds.

Basis Light-Front Quantization: Recent Progress and Future Prospects

2016 ◽  
Vol 57 (8) ◽  
pp. 695-702 ◽  
Author(s):  
James P. Vary ◽  
Lekha Adhikari ◽  
Guangyao Chen ◽  
Yang Li ◽  
Pieter Maris ◽  
...  
Keyword(s):  
Recent Progress ◽  
Light Front ◽  
Future Prospects ◽  
Light Front Quantization

scholarly journals The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 430 ◽  
Author(s):  
Anja Sadžak ◽  
Janez Mravljak ◽  
Nadica Maltar-Strmečki ◽  
Zoran Arsov ◽  
Goran Baranović ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Structural Changes ◽  
Structural Integrity ◽  
Lipid Membrane ◽  
Membrane Properties ◽  
Structural Reorganization ◽  
Unsaturated Lipids ◽  
Oxidative Attack

The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.

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