scholarly journals Recent Lipid Membrane-Based Biosensing Platforms

2019 ◽  
Vol 9 (9) ◽  
pp. 1745 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Christina G. Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Spyridoula Bratakou ◽  
Dimitrios K. Bendos
Keyword(s):  
Lipid Membrane ◽  
Clinical Chemistry ◽  
Environmental Pollutants ◽  
Membrane Technology ◽  
Lipid Film ◽  
Wide Range ◽  
Lipid Films ◽  
Clinical Interest ◽  
Food Toxicants

The investigation of lipid films for the construction of biosensors has recently given the opportunity to manufacture devices to selectively detect a wide range of food toxicants, environmental pollutants, and compounds of clinical interest. Biosensor miniaturization using nanotechnological tools has provided novel routes to immobilize various “receptors” within the lipid film. This chapter reviews and exploits platforms in biosensors based on lipid membrane technology that are used in food, environmental, and clinical chemistry to detect various toxicants. Examples of applications are described with an emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The compounds that can be monitored are insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc.

scholarly journals The Application of Lipid Membranes in Biosensing

Membranes ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 108 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Dimitrios Nikolelis ◽  
Christina Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Food Quality ◽  
Lipid Membranes ◽  
Recent Progress ◽  
Lipid Membrane ◽  
Environmental Pollutants ◽  
Quality Monitoring ◽  
Future Prospects ◽  
Considerable Part ◽  
Lipid Film ◽  
Food Toxicants

The exploitation of lipid membranes in biosensors has provided the ability to reconstitute a considerable part of their functionality to detect trace of food toxicants and environmental pollutants. This paper reviews recent progress in biosensor technologies based on lipid membranes suitable for food quality monitoring and environmental applications. Numerous biosensing applications based on lipid membrane biosensors are presented, putting emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The range of analytes that can be currently using these lipid film devices that can be detected include, insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc. Technology limitations and future prospects are discussed, focused on the evaluation/validation and eventually commercialization of the proposed lipid membrane-based biosensors.

scholarly journals Nanotechnological advances of Lipid film-based biosensors for the rapid detection of biological compounds and toxicants

2019 ◽  
pp. 32-40
Author(s):  
Georgia-Paraskevi Nikoleli
Keyword(s):  
Lipid Membranes ◽  
Lipid Membrane ◽  
Cholesterol Oxidase ◽  
Environmental Pollutants ◽  
Clinical Analysis ◽  
Lipid Film ◽  
Biological Interest ◽  
Wide Range ◽  
Biological Compounds ◽  
Novel Devices

The exploration of lipid membranes for the construction of nanobiosensors has recently provided the opportunity to construct devices to monitor a wide range of compounds of biological interest. Nanobiosensor miniaturization using nanotechnological tools has given novel ways to attach a wide range of “receptors” in the lipid membrane. The lipids used to construct a lipid film-based device are dipalmiloylphosphatidylcholine {DPPC} and in some cases dipalmitoylphosphatidic acid (DPPA) which is an anionic lipid and is used to increase the sensitivity of detection. Most common “receptors” used in lipid film biosensors are enzymes such as urease, cholesterol oxidase, urecase, etc, antibodies such as D-dimer antibody and artificial or natural receptors such as saxitoxin, cholera toxin, calyx [4] arene phospjoryl receptor, etc. This chapter reviews and investigates the construction of nanobiosensors based on lipid membranes that are used to monitor various toxicants. It also exploits examples of applications with an emphasis on novel devices, new nanobiosensing techniques and nanotechnology-based transduction schemes. The compounds that can be detected are insecticides, toxins, hormones, dioxins, etc. Keywords: Lipid membrane based nanosensors; Nanoyechology; Graphene and ZnO electrodes; Food toxicants; Environmental pollutants; Clinical analysis

scholarly journals Lipid Membrane Based Biosensors

2018 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Dimitrios P. Nikolelis ◽  
Christina G. Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Food Quality ◽  
Lipid Membranes ◽  
Recent Progress ◽  
Lipid Membrane ◽  
Environmental Pollutants ◽  
Quality Monitoring ◽  
Future Prospects ◽  
Considerable Part ◽  
Bilayer Device ◽  
Food Toxicants

The exploitation of lipid membranes in biosensors has provided the ability to reconstitute a considerable part of their functionality to detect trace of food toxicants and environmental pollutants. Nanotechnology enabled sensor miniaturization and extended the range of biological moieties that could be immobilized within a lipid bilayer device. This chapter reviews recent progress in biosensor technologies based on lipid membranes suitable for environmental applications and food quality monitoring. Numerous biosensing applications are presented, putting emphasis on novel systems, new sensing techniques and nanotechnology-based transduction schemes. The range of analytes that can be currently detected include, insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc. Technology limitations and future prospects are discussed, focused on the evaluation/ validation and eventually commercialization of the proposed sensors.

scholarly journals Potentiometric Biosensing Applications of Graphene Electrode with Stabilized Polymer Lipid Membranes

2018 ◽  
Author(s):  
Georgia-Pareaskevi Nikoleli ◽  
Dimitrios P. Nikolelis ◽  
Christina G. Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Lipid Membranes ◽  
Rapid Detection ◽  
Toxic Substance ◽  
Environmental Pollutants ◽  
High Sensitivity ◽  
Fast Response ◽  
Toxic Compounds ◽  
Wide Range ◽  
Sensitivity And Selectivity ◽  
Clinical Interest

This review provides informations and details for the fabrication of biosensors that are composed from lipid membranes and have been utilized and applied to rapidly detect food toxic compounds, environmental pollutants and analytes of clinical interest. Biosensors based on polymeric lipid membranes have been used to rapidly detect a wide range of these analytes and offer several advantages such as fast response, high sensitivity and selectivity, can be portable for in the field applications, and small size. A description of the construction of these devices and their applications for the rapid detection of food toxic substance, environmental pollutants and analytes of clinical interest is provided in this review.

scholarly journals Ανάπτυξη και αξιολόγηση μεθόδων προσδιορισμού PCBs στα τρόφιμα

2012 ◽  
Author(s):  
Αντώνιος Μιχαλολιάκος
Keyword(s):  
Electrolyte Solution ◽  
Rapid Detection ◽  
Active Sites ◽  
Lipid Membrane ◽  
Flow Mode ◽  
Raman Spectrometry ◽  
Lipid Film ◽  
Real Samples ◽  
Wide Range ◽  
Aroclor 1242

Polychlorinated biphenyls (PCBs) are industrial compounds which are known to be among the most persistent and widely distributed pollutants in the global ecosystem. Since they are lipophilic, they tend to bioaccumulate in the fatty tissues of living organisms, including humans. Owing to the chemical and physical properties of PCBs, the analysis by conventional methods is difficult and expensive. Therefore simple methods for the detection of PCBs in environmental, industrial and food samples are required. The Ph.D. thesis involved the study, design and construction of electrochemical bilayer lipid membrane-based biosensors for rapid detection of compounds of biomedical, environmental and industrial interest such as PCBs. This work describes a novel electrochemical biosensor based on a supported polymerized lipid film with incorporated Sheep anti-PCB antibody for the rapid detection of aroclor 1242, at the levels of 10-9 M concentrations, in flowing solution streams. The antibody was incorporated into the lipid film during polymerization. Injections of Aroclor 1242 antigen solutions were made into flowing streams of a carrier electrolyte solution. Experiments were done in a stopped-flow mode using lipid mixtures containing 15% (w/w) dipalmitoylphosphatidic acid (DPPA) to provide only a single transient current signal with a magnitude related to the antigen concentration. An immunosensor based on the BLM transduction scheme should be regenerable and capable of multiple analyses. Thus, lipid films containing 35% DPPA were used to examine regeneration of the active sites of antibody after complex formation by washing with the carrier electrolyte solution. Repetitive cycles of injection of antigen have shown that the maximum number of cycles is about 5. The mechanism of signal generation was investigated by physicochemical methods of IR, Raman spectrometry and Scanning Electron Microscopy. The device was tested/ evaluated in real samples of vegetables. The investigation of the effect of potent interferences included a wide range of compounds usually found in foods. The results showed no interferences from these compounds in concentration levels usually found in real samples. The analyses that have been concluded in order to define the PCBs in the foods as well as the comparison of these results against the results of other established methods have proved that the biosensor used provides reliable results and it can therefore constitute a valuable tool for future applications in the field of Environmental Chemistry.

scholarly journals Nanotechnological advances of Lipid film based biosensors for the rapid detection of biological compounds and toxicants

2020 ◽  
pp. 27-35
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Keyword(s):  
Lipid Membranes ◽  
Rapid Detection ◽  
Lipid Membrane ◽  
Cholesterol Oxidase ◽  
Lipid Film ◽  
Biological Interest ◽  
Wide Range ◽  
Biological Compounds ◽  
Novel Devices ◽  
D Dimer

The exploration of lipid membranes for the construction of nanobiosensors has recently provided the opportunity to construct devices to monitor a wide range of compounds of biological interest. Nanobiosensor miniaturization using nanotechnological tools has given novel ways to attach a wide range of “receptors” in the lipid membrane. The lipids used to construct a lipid film based device are dipalmiloylphosphatidylcholine {DPPC} and in some cases dipalmitoylphosphatidic acid (DPPA) which is an anionic lipid and is used to increase the sensitivity of detection. Most common “receptors” used in lipid film biosensors are enzymes such as urease, cholesterol oxidase, urecase, etc, antibodies such as D-dimer antibody and artificial or natural receptors such as saxitoxin, cholera toxin, calyx[4]arene phospjoryl receptor, etc. This chapter reviews and investigates the construction of nanobiosensors based on lipid membranes that are used to monitor various toxicants. It also exploits examples of applications with an emphasis on novel devices, new nanobiosensing techniques and nanotechnology-based transduction schemes. The compounds that can be detected are insecticides, toxins, hormones, dioxins, etc.

scholarly journals Lipid Membrane Based Biosensors

2018 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Dimitrios Nikolelis ◽  
Christina Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Food Quality ◽  
Lipid Membranes ◽  
Recent Progress ◽  
Lipid Membrane ◽  
Environmental Pollutants ◽  
Quality Monitoring ◽  
Future Prospects ◽  
Considerable Part ◽  
Bilayer Device ◽  
Food Toxicants

The exploitation of lipid membranes in biosensors has provided the ability to reconstitute a considerable part of their functionality to detect trace of food toxicants and environmental pollutants. Nanotechnology enabled sensor miniaturization and extended the range of biological moieties that could be immobilized within a lipid bilayer device. This chapter reviews recent progress in biosensor technologies based on lipid membranes suitable for environmental applications and food quality monitoring. Numerous biosensing applications are presented, putting emphasis on novel systems, new sensing techniques and nanotechnology-based transduction schemes. The range of analytes that can be currently detected include, insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc. Technology limitations and future prospects are discussed, focused on the evaluation/ validation and eventually commercialization of the proposed sensors.

scholarly journals Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles

Membranes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Damian Dziubak ◽  
Kamil Strzelak ◽  
Slawomir Sek
Keyword(s):  
Electrochemical Properties ◽  
Self Assembly ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Membrane Resistance ◽  
Electrochemical Characteristics ◽  
Freeze Thaw ◽  
Lipid Films ◽  
Supported Lipid Membranes

Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance.

scholarly journals Gold–Carbon Nanocomposites for Environmental Contaminant Sensing

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 719
Author(s):  
Shahrooz Rahmati ◽  
William Doherty ◽  
Arman Amani Babadi ◽  
Muhamad Syamim Akmal Che Mansor ◽  
Nurhidayatullaili Muhd Julkapli ◽  
...  
Keyword(s):  
Environmental Pollutants ◽  
High Sensitivity ◽  
Environmental Crisis ◽  
World Population ◽  
Chemical Contaminants ◽  
Carbon Nanocomposites ◽  
Minimal Sample ◽  
Wide Range ◽  
And Control ◽  
Analytical Tools

The environmental crisis, due to the rapid growth of the world population and globalisation, is a serious concern of this century. Nanoscience and nanotechnology play an important role in addressing a wide range of environmental issues with innovative and successful solutions. Identification and control of emerging chemical contaminants have received substantial interest in recent years. As a result, there is a need for reliable and rapid analytical tools capable of performing sample analysis with high sensitivity, broad selectivity, desired stability, and minimal sample handling for the detection, degradation, and removal of hazardous contaminants. In this review, various gold–carbon nanocomposites-based sensors/biosensors that have been developed thus far are explored. The electrochemical platforms, synthesis, diverse applications, and effective monitoring of environmental pollutants are investigated comparatively.

scholarly journals Size matters: Size Dependency of Gold Nanoparticles Interacting with Model Membranes

2019 ◽  
Author(s):  
Claudia Contini ◽  
James W. Hindley ◽  
Tom Macdonald ◽  
Joseph Barritt ◽  
Oscar Ces ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lipid Bilayers ◽  
Lipid Membrane ◽  
Rapid Development ◽  
Membrane System ◽  
Size Dependent ◽  
Research Fields ◽  
Large Unilamellar Vesicles ◽  
Wide Range ◽  
Experimental Characterisation

<p><b>The rapid development of nanomaterials has led to an increase in the number and variety of engineered nanomaterials (ENMs) in the environment. Gold nanoparticles (AuNPs) are an example of a commonly studied ENM whose highly tailorable properties have generated significant interest through a wide range of research fields. In the present work, we report the first qualitative as well as quantitative experimental characterisation of the AuNP-membrane interaction. We investigate the interactions between citrate-stabilised AuNPs (diameters 5, 10, 25, 35, 50, 60 nm) and large unilamellar vesicles (LUVs) acting as a model membrane system. LUVs were prepared in two different formulations using 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and 1,2-dileoyl-sn-glycero-3-phosphocholine (DOPC). Our results show that the interaction between AuNPs and LUVs is size dependent; in particular, we reveal the existence of two AuNP’s critical diameters which determine the fate of AuNPs in contact with a lipid membrane. The results provide a new understanding of the size dependent interaction between AuNPs and lipid bilayers of direct relevance to nanotoxicology and to the design of NP vectors.</b></p>

Sign in / Sign up

Export Citation Format

Share Document