A Glucose Biosensor based on Horseradish Peroxidase and Glucose Oxidase Co-entrapped in Carbon Nanotubes Modified Electrode

Author(s):  
Han Yang ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 3059-3064
Author(s):  
A. K. M. Kafi ◽  
Aizam Bin Kasri ◽  
Rajan Jose

The fabrication of a network of glucose oxidase-horseradish peroxidase/tin oxide (GOx-HRP/SnO2), immobilized onto a glassy carbon electrode (GCE) and its utilization as a biosensor for glucose detection is reported. The network established with GOx-HRP/SnO2 possess high sensitivity and stability by performing the electrocatalytic features in the sensing of glucose. The turbidity of fabrication had been scanned and analyzed using UV-vis spectroscopy. The morphology and composition of the fabricated GOx-HRP/SnO2 networks were characterized by scanning electron microscopy (SEM). Cyclic voltammetry and amperometry were employed to study the electrochemical properties of the proposed biosensor. The effect of applied electrode potential and pH were systemically investigated. The biosensor responds to glucose at work potential values between −400 mV, and exhibited a lower detection limit (0.025 mM) and long linear range (0.25 to 7.0 mM), and was resistant to common interferences.


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