ETHNO MEDICINAL SURVEY AND EVALUATION OF TWO RECIPES USED IN MANAGING SICKLE CELL DISEASE IN ILE-IFE COMMUNITY OF OSUN-STATE, NIGERIA

2020 ◽  
Vol 17 (2) ◽  
pp. 37-54
Author(s):  
Mojisola Christianah Cyril-Olutayo ◽  
Dorcas O. Ajayi ◽  
Olayinka O Odunowo
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Aqueous Extract ◽  
Negative Control ◽  
Cell Disease ◽  
Reversal Agent ◽  
Medical Practitioners ◽  
Survey And Evaluation ◽  
Phosphate Buffered Saline ◽  
Positive Controls

Background: Ethno-medicinal survey of herbal recipes used in managing sickle cell disease in Ile-Ife, Osun-State, Nigeria was carried out and two recipes selected for antisickling studies. Materials and Methods: Information was obtained using semi-structured questionnaires and open interview from respondents consisting of herb sellers, traditional medical practitioners, artisans and traders in two local government areas of Ife. Two recipes from the survey were selected for antisickling studies. Aqueous extract of each recipe was obtained by boiling the constituents in water for 1 h while the hydro ethanolic extracts were obtained by maceration in 70% ethanol for 72 h. Inhibitory and reversal antisickling properties were assessed using sodium metabisulphite as deoxygenating agent, vanillic acid (inhibitory agent), para-hydroxybenzoic (reversal agent) acid as positive controls while phosphate buffered saline was employed as negative control. Results: Fifty four recipes comprising forty six plants were obtained from the ethno-medicinal survey. The respondents comprised of 44% men and 56% women. The most frequently and commonly used plants belong to family Fabaceae. The inhibitory and reversal activities of the aqueous extract of recipe 1 (81.37± 1.09%, 88.56 ±1.38% respectively) were significantly (p < 0.05) higher than recipe 2 (78.51 ± 0.78% inhibition and 79.8 ± 2.16% reversal) at same concentration. The hydro-alcoholic extracts of recipes 1 and 2 gave highest inhibitory activities at 0.5 mg/mL (69.25 ±1.30% and 68.28 ±2.78% respectively). Conclusion: This study documented the medicinal plants and recipes used in Ile-Ife for managing sickle cell disease, and validated the ethno-medicinal claim of two recipes.

Assessment of sickling inhibitory activity of Ficus carpensis, Newbouldia laevis, Carpolobia lutea and Carpolobia caudata on human erythrocytes HbSS

2021 ◽  
Vol 10 (1) ◽  
pp. 15-18
Author(s):  
Jean François Sibri ◽  
◽  
Joël Akakpo-Akue ◽  
Jacques Auguste Alfred Bognan Ackah ◽  
M'Bèféhê Soro ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Chemical Study ◽  
Cell Disease ◽  
Traditional Treatment ◽  
Anti Oxidant ◽  
The Subject ◽  
Increased Susceptibility

Sickle cell disease is a hemoglobinopathy. In hypoxia situation, homozygous individuals might suffer from vaso-occlusive seizures, hemolytic anemia and increased susceptibility to infections. A recipe of the leaves of Ficus carpensis, Newbouldia laevis, Carpolobia lutea and Carpolobia caudate, four plants used in the traditional treatment of sickle cell disease in the Daloa region, was the subject of this study. This investigation was initiated in order to assess sickle cell inhibitory activity of the recipe, by using Emmel’s method. The decocted (DS) and the aqueous extract (EA) of the recipe brought the residual sickle cell rate at 10 and 11% respectively. Which correspond to sickle cell inhibitory activity of 90% for the decocted (DS) and 89% for the aqueous extract (EA). These activities were higher than that of phenylalanine (83%) which is used as an antisickling amino acid reference. The chemical study of the both extracts of the recipe revealed the presence of compounds known for their anti-oxidant and anti-sickle cell activities. Those flavonoids, polyphenols and alkaloids could be partly responsible for the sickle cell inhibitory activity of the recipe. These results showed that both the extracts of the recipe had antisickling activity. The use of this recipe of four plants in the traditional treatment of sickle cell disease in the Daloa region might be justified.

Effect of Rhynchospora corymbosa and Olax subscorpioïdea two plants used in the management of Korhogo sickle cell disease

2021 ◽  
Vol 10 (1) ◽  
pp. 1-6
Author(s):  
M'béfèhê Soro ◽  
◽  
Joël Akakpo-Akue ◽  
Jacques Auguste Alfred Bognan Ackah ◽  
Jean-François Sibri ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Aqueous Extract ◽  
Chemical Study ◽  
Cell Disease ◽  
Traditional Management ◽  
Sickle Cells ◽  
Anti Oxidant ◽  
Chemical Groups

Sickle cell disease is the first genetic disease in the world. Rhynchospora corymbosa and Olax subscorpioïdea are two plant species used in traditional management of sickle cell disease in the department of Korhogo. After a chemical study of the decocted and the aqueous extract of these two plants, their effect on the in vitro reversal of sickling of HbSS genotype erythrocytes, has been evaluated according to Emmel method. An abundance of alkaloids, tannins, saponins, polyterpenes and sterols was found in the decocted and aqueous extract of Rhynchospora corymbosa (DRC and MRC). As for the decocted and aqueous extract of Olax subscorpioïdea (DOSA and MOSA), polyphenols, leucoanthocyanins and flavonoids were found in addition to the chemical compounds identified in Rhynchospora corymbosa. At the concentration of 0.312 mg/mL DRC, MRC DOSA and MOSA have obtained the reversal rate of 75, 50, 80 and 80% respectively. All four extracts demonstrated activity on in vitro reversal of sickle cells. This activity could be caused by the presence of chemical groups that would be used in the treatment of several diseases because of their anti-oxidant and anti-inflammatory properties. Rhynchospora corymbosa and Olax subscorpioïdea are both an effect on the in vitro sickling reversal. Their use in the management of sickle cell disease in northern Côte d'Ivoire might be justified.

scholarly journals Comparative studies on antisickling properties of brown and green leaves of Carica papaya Linn. (Caricaceae)

2017 ◽  
Vol 9 (1) ◽  
pp. 38
Author(s):  
Glory Oluremilekun Ajayi ◽  
Ogun Olupelumi Maria
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Carica Papaya ◽  
Isotonic Saline ◽  
Ultra Violet ◽  
Positive Control ◽  
Negative Control ◽  
Herbal Remedies ◽  
Cell Disease ◽  
Green Leaves

<p>Sickle Cell Disease (SCD) is characterised by non-covalent polymerisation of the haemoglobin under hypoxia conditions and this promotes red blood cell sickling. Inhibition of sickle cell haemoglobin polymerization is one of the areas of focus in the management of SCD. Dried <em>Carica papaya</em> leaves are used in traditional herbal remedies for the management of sickle cell anaemia; without specifying if it is the dried green or brown leaf.</p><p> This study was aimed at verifying the antisickling activity of the crude aqueous extract, crude methanol extract and fractions of dried brown and green leaves of <em>Carica papaya</em>.</p><p>The method used was the sickle cell haemoglobin polymerization inhibition experiment<strong> </strong>measured with the Ultra Violet (UV) spectrophotometer. Sodium metabisulphite was used as a deoxygenating agent while isotonic saline (0.9% NaCl) was used as the negative control and phenylalanine as a positive control. The results obtained showed that crude aqueous extracts of both the green and brown leaves exhibited high level of inhibition of sickled haemoglobin (HbSS) polymerization at 200 mg/ml (97.76% and 93.25% ), 100 mg/ml (95.89% and 97.93%) also, 50 mg/ml (97.89% and 95.84%) respectively which compared favourably and significantly (p&lt;0.05) with that of phenylalanine.</p><p>The summary of the antisickling activity of the crude extracts and fractions of both the green leaves and brown leaves of <em>C. papaya</em> is Crude aqueous&gt;Crude aqueous methanol&gt; Butanol&gt;Chloroform&gt;Ethyl acetate&gt;Aqueous. This study showed that the extracts exhibited the potential of inhibiting polymerization of sickle cell haemoglobin thus would be beneficial in the management of sickle cell disease.</p>

Hemolysis in sickle cell disease

1974 ◽  
Vol 133 (4) ◽  
pp. 624-631 ◽  
Author(s):  
T. A. Bensinger
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Cell Disease

Letter: Sickle cell disease and the kidney: a hypothesis

1974 ◽  
Vol 134 (1) ◽  
pp. 181a-181
Author(s):  
D. C. Salyer
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Cell Disease

Computerized cognitive training in pediatric sickle cell disease: A randomized controlled pilot study.

2020 ◽  
Vol 8 (4) ◽  
pp. 390-401 ◽  
Author(s):  
Taryn M. Allen ◽  
Lindsay M. Anderson ◽  
Samuel M. Brotkin ◽  
Jennifer A. Rothman ◽  
Melanie J. Bonner
Keyword(s):  
Pilot Study ◽  
Sickle Cell Disease ◽  
Cognitive Training ◽  
Sickle Cell ◽  
Cell Disease ◽  
Computerized Cognitive Training ◽  
Randomized Controlled ◽  
Pediatric Sickle Cell Disease
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