Assessment of sickling inhibitory activity of Ficus carpensis, Newbouldia laevis, Carpolobia lutea and Carpolobia caudata on human erythrocytes HbSS

2021 ◽  
Vol 10 (1) ◽  
pp. 15-18
Author(s):  
Jean François Sibri ◽  
◽  
Joël Akakpo-Akue ◽  
Jacques Auguste Alfred Bognan Ackah ◽  
M'Bèféhê Soro ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Chemical Study ◽  
Cell Disease ◽  
Traditional Treatment ◽  
Anti Oxidant ◽  
The Subject ◽  
Increased Susceptibility

Sickle cell disease is a hemoglobinopathy. In hypoxia situation, homozygous individuals might suffer from vaso-occlusive seizures, hemolytic anemia and increased susceptibility to infections. A recipe of the leaves of Ficus carpensis, Newbouldia laevis, Carpolobia lutea and Carpolobia caudate, four plants used in the traditional treatment of sickle cell disease in the Daloa region, was the subject of this study. This investigation was initiated in order to assess sickle cell inhibitory activity of the recipe, by using Emmel’s method. The decocted (DS) and the aqueous extract (EA) of the recipe brought the residual sickle cell rate at 10 and 11% respectively. Which correspond to sickle cell inhibitory activity of 90% for the decocted (DS) and 89% for the aqueous extract (EA). These activities were higher than that of phenylalanine (83%) which is used as an antisickling amino acid reference. The chemical study of the both extracts of the recipe revealed the presence of compounds known for their anti-oxidant and anti-sickle cell activities. Those flavonoids, polyphenols and alkaloids could be partly responsible for the sickle cell inhibitory activity of the recipe. These results showed that both the extracts of the recipe had antisickling activity. The use of this recipe of four plants in the traditional treatment of sickle cell disease in the Daloa region might be justified.

Effect of Rhynchospora corymbosa and Olax subscorpioïdea two plants used in the management of Korhogo sickle cell disease

2021 ◽  
Vol 10 (1) ◽  
pp. 1-6
Author(s):  
M'béfèhê Soro ◽  
◽  
Joël Akakpo-Akue ◽  
Jacques Auguste Alfred Bognan Ackah ◽  
Jean-François Sibri ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Aqueous Extract ◽  
Chemical Study ◽  
Cell Disease ◽  
Traditional Management ◽  
Sickle Cells ◽  
Anti Oxidant ◽  
Chemical Groups

Sickle cell disease is the first genetic disease in the world. Rhynchospora corymbosa and Olax subscorpioïdea are two plant species used in traditional management of sickle cell disease in the department of Korhogo. After a chemical study of the decocted and the aqueous extract of these two plants, their effect on the in vitro reversal of sickling of HbSS genotype erythrocytes, has been evaluated according to Emmel method. An abundance of alkaloids, tannins, saponins, polyterpenes and sterols was found in the decocted and aqueous extract of Rhynchospora corymbosa (DRC and MRC). As for the decocted and aqueous extract of Olax subscorpioïdea (DOSA and MOSA), polyphenols, leucoanthocyanins and flavonoids were found in addition to the chemical compounds identified in Rhynchospora corymbosa. At the concentration of 0.312 mg/mL DRC, MRC DOSA and MOSA have obtained the reversal rate of 75, 50, 80 and 80% respectively. All four extracts demonstrated activity on in vitro reversal of sickle cells. This activity could be caused by the presence of chemical groups that would be used in the treatment of several diseases because of their anti-oxidant and anti-inflammatory properties. Rhynchospora corymbosa and Olax subscorpioïdea are both an effect on the in vitro sickling reversal. Their use in the management of sickle cell disease in northern Côte d'Ivoire might be justified.

Procedures, Pain, and Parents

PEDIATRICS ◽  
1991 ◽  
Vol 87 (4) ◽  
pp. 563-565
Author(s):  
HOWARD BAUCHNER
Keyword(s):  
Sickle Cell Disease ◽  
Lumbar Puncture ◽  
Sickle Cell ◽  
Acute Pain ◽  
Cell Disease ◽  
Emergency Rooms ◽  
Pharmacological Agents ◽  
The Past ◽  
The Subject

During the past decade certain types of pain in children have been the subject of much research and discussion. The pain associated with cancer, sickle cell disease, and the preoperative and post-operative periods have all been extensively studied and reviewed.1-4 Less information is available about acute pain inflicted in emergency rooms. Children commonly undergo procedures such as venipuncture, intravenous cannulation, lumbar puncture, and manipulation of fractures in emergency rooms without the benefit of any analgesia. What techniques are available to reduce the pain and anxiety that children feel when they undergo procedures? Traditionally, physicians have tried to reduce pain by using pharmacological agents.

ETHNO MEDICINAL SURVEY AND EVALUATION OF TWO RECIPES USED IN MANAGING SICKLE CELL DISEASE IN ILE-IFE COMMUNITY OF OSUN-STATE, NIGERIA

2020 ◽  
Vol 17 (2) ◽  
pp. 37-54
Author(s):  
Mojisola Christianah Cyril-Olutayo ◽  
Dorcas O. Ajayi ◽  
Olayinka O Odunowo
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Aqueous Extract ◽  
Negative Control ◽  
Cell Disease ◽  
Reversal Agent ◽  
Medical Practitioners ◽  
Survey And Evaluation ◽  
Phosphate Buffered Saline ◽  
Positive Controls

Background: Ethno-medicinal survey of herbal recipes used in managing sickle cell disease in Ile-Ife, Osun-State, Nigeria was carried out and two recipes selected for antisickling studies. Materials and Methods: Information was obtained using semi-structured questionnaires and open interview from respondents consisting of herb sellers, traditional medical practitioners, artisans and traders in two local government areas of Ife. Two recipes from the survey were selected for antisickling studies. Aqueous extract of each recipe was obtained by boiling the constituents in water for 1 h while the hydro ethanolic extracts were obtained by maceration in 70% ethanol for 72 h. Inhibitory and reversal antisickling properties were assessed using sodium metabisulphite as deoxygenating agent, vanillic acid (inhibitory agent), para-hydroxybenzoic (reversal agent) acid as positive controls while phosphate buffered saline was employed as negative control. Results: Fifty four recipes comprising forty six plants were obtained from the ethno-medicinal survey. The respondents comprised of 44% men and 56% women. The most frequently and commonly used plants belong to family Fabaceae. The inhibitory and reversal activities of the aqueous extract of recipe 1 (81.37± 1.09%, 88.56 ±1.38% respectively) were significantly (p < 0.05) higher than recipe 2 (78.51 ± 0.78% inhibition and 79.8 ± 2.16% reversal) at same concentration. The hydro-alcoholic extracts of recipes 1 and 2 gave highest inhibitory activities at 0.5 mg/mL (69.25 ±1.30% and 68.28 ±2.78% respectively). Conclusion: This study documented the medicinal plants and recipes used in Ile-Ife for managing sickle cell disease, and validated the ethno-medicinal claim of two recipes.

scholarly journals Aged Neutrophils in Sickle Cell Disease Impede Ischemic Repair

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 3688-3688
Author(s):  
Derick Okwan-Duodu ◽  
David R. Archer ◽  
W Robert Taylor
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Conflicts Of Interest ◽  
Oxidant Stress ◽  
Limb Ischemia ◽  
Wild Type ◽  
Cell Disease ◽  
Tissue Ischemia ◽  
Anti Oxidant ◽  
And Function

Abstract INTRODUCTION: Repeated bout of tissue ischemia is a hallmark of sickle cell disease (SCD). Neutrophils are critical first responder cells that initiate host response to sterile injury, including ischemia. Of the numerous neutrophils in circulation, a small proportion undergo physiologic changes (termed aging) which directs their homing to the bone marrow for clearance. How neutrophil homeostasis is regulated in sickle cell disease (SCD) to influence ischemic repair is unknown. HYPOTHESIS: We hypothesized that SCD may alter the homeostasis and function of aged neutrophil. METHODS: Aged neutrophils from the Townes humanized sickle cell (SS) mice were compared to aged neutrophils from MerTK deficient mice (MerKO), which age in the circulation owing to defective efferocytosis. Hind limb ischemia (HLI) was used as model of vascular injury. LASER Doppler perfusion imaging (LDPI) measured perfusion recovery after HLI. RESULTS: Compared to wild-type mice (7 ± 1.2%), the proportion of CXCR4HiCD62Llo aged neutrophils in circulation was markedly elevated in the SS (34 ± 4%) and MerKO mice (38 ± 4%). However, in contrast with aged neutrophils from MerKO mice, those from SS mice showed impaired phagocytosis, increased capacity to produce reactive oxygen species, and pronounced release of extracellular traps (NETs). In response to HLI, perfusion recovery in the MerKO and wild type mice were similar, but this was dramatically impaired in SS mice. Importantly, the phenotype of SS mice was reversed by treatment with the anti-oxidant N-acetyl cysteine (NAC), but NAC therapy had no impact on MerKO mice. CONCLUSIONS: Although SS and MerKO mice demonstrated elevated numbers of aged neutrophils, our data suggest that oxidant stress causes aged CXCR4HiCD62Llo neutrophils in SS to impede vascular repair after ischemia. This specialized subset of neutrophils may be targeted to improve vascular health in SCD. Disclosures No relevant conflicts of interest to declare.

scholarly journals The Impact of Selenium Deficiency on a Sickle Cell Disease Mouse Model

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 3645-3645
Author(s):  
Ramasamy Jagadeeswaran ◽  
Hong Lenny ◽  
Helen Zhang ◽  
Jennifer Afranie-Sakyi ◽  
Robert E. Molokie ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
African Americans ◽  
Sickle Cell Disease ◽  
Mouse Model ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Blood Cells ◽  
Cell Disease ◽  
Anti Oxidant ◽  
The Impact

Abstract Sickle cell disease (SCD) is caused by a mutation in the β-globin gene resulting in a disease that affects more than 100,000 Americans and millions worldwide. Though pain is the hallmark of SCD, patients also suffer damage to most organ systems. Sickle cell hemoglobin (HbS) polymerization occurs when deoxygenated, rendering red blood cells rigid and fragile. Production of excessive reactive oxygen species (ROS) and intracellular hypoxia in RBCs further accelerates the pathology associated with SCD. Recently, vaso-occlusive crisis (VOC) and organ damage were established to be strongly associated with oxidative stress in RBCs. This occurs when there is an increase in oxidants that exceeds the cellular anti-oxidant defenses. Excessive ROS can trigger a cascade of oxidative reactions that damage membrane lipids, and essential enzymatic antioxidants such as GPx-1, ultimately leading to hemolysis and multi-organ dysfunction. ROS generation in RBCs of SCD patients is due to factors such as HbS auto-oxidation and potentially aberrant mitochondrial function. We recently determined that red blood cells obtained from SCD mice and SCD patients retain their mitochondria compared to control subjects. Mitochondria retained SCD RBCs are also associated with elevated levels of ROS and hemolysis. Oxidative stress in the RBCs of SCD patients may be elevated by lower levels of antioxidant proteins such as the selenium-dependent enzyme GPX1. GPX1 was first described as an enzyme capable of protecting hemoglobin from ROS and has been reported to be lower in the RBCs in SCD. Selenium levels are lower among African Americans in the Chicago area and elsewhere. In this regard, it is notable that in the United States, African Americans represent the majority of those with SCD. To investigate the relationship between selenium levels and SCD, we have utilized a mouse model of SCD to examine the impact of a reduced intake of selenium on parameters associated with SCD pathology. SCD mice on a selenium-deficient diet (<0.01 mg/kg diet) were compared to mice fed with a selenium-adequate diet (0.1mg/kg). SCD mice in the selenium-deficient group exhibited an increase in mitochondria retaining RBCs (Se deficient: 26%±6.9%, n=3 vs. Se adequate: 5 % ± 3.5%, n=3, p<0.01), reduced Hb levels (Se deficient 5.7± 0.17 g/dl, n=3 vs. Se adequate 7.0± 0.83 g/dl, n=4 p<0.05), and an increased RBC oxygen consumption rate (OCR). These results support the hypothesis that low selenium status likely results in reduced levels of anti-oxidant selenoproteins and enhanced SCD severity. Disclosures Lavelle: Global Blood therapeutics: Research Funding.

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