scholarly journals Development of a Latex Agglutination Test for Detecting Pathogenic Burkholderia and its Approbation in the Endemic Regions of Vietnam

2021 ◽  
pp. 133-138
Author(s):  
D. M. Frolov ◽  
N. N. Teteryatnikova ◽  
T. L.A. Bui ◽  
I. B. Zakharova ◽  
N. P. Khrapova
Keyword(s):  
Burkholderia Cepacia ◽  
High Sensitivity ◽  
Polymyxin B ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Antigenic Structure ◽  
Bacterial Cultures ◽  
Freeze Dried ◽  
Latex Test

The aim of the work was development of a monoclonal antibody-based latex agglutination test to identify the causative agent of melioidosis, and the approbation of a freeze-dried experimental preparation for screening of environmental bacterial isolates in Vietnam.Materials and methods. The carriers of specific antibodies were polyacrolein latex particles with active aldehyde groups on the surface. Typical strains of the causative agents of melioidosis and glanders with a full-fledged antigenic structure, as well as the strains Burkholderia thailandensis, Burkholderia cepacia, Pseudomonas aeruginosa, and Pseudomonas putida were used to control the test specificity. The latex agglutination reaction was carried out on plastic Petri dishes with daily bacterial cultures, from which suspensions were prepared at a concentration of 1–2·109 m.c./ml. The results of the reaction were registered visually for 5–8 min using a 4-cross system against a dark background under lighting. The reaction to 3–4 crosses was recorded as positive. Colonies suspected of belonging to pathogenic Burkholderia from primary inoculations were transferred to L-agar with polymyxin B and grown for 36 hours at (37±1) °C. The species of the selected suspicious colonies was determined by multiplex PCR.Results and discussion. With collection strains, latex test demonstrated high sensitivity agglutinating 97.7 % of B. pseudomallei and all B. mallei strains. At the same time, it was negative with B. thailandensis, B. cepacia, P. aeruginos and P. putida. In microbiological screening of bacterial cultures isolated from environmental objects, the latex test had a diagnostic sensitivity of 89.4 %. Using the latex test at the stage of primary screening, it is possible to significantly reduce the time when processing a lot of samples received for analysis, as well as to reduce the consumption of reagents used at the subsequent stages of identification. 

Evaluation of a Latex Agglutination Test for Rapid Identification of Staphylococcus aureus from Foods

1993 ◽  
Vol 56 (9) ◽  
pp. 759-762 ◽  
Author(s):  
TSUNG C. CHANG ◽  
SU H. HUANG
Keyword(s):  
Staphylococcus Aureus ◽  
Immunoglobulin G ◽  
Protein A ◽  
Rapid Identification ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Polystyrene Latex ◽  
Latex Agglutination Test ◽  
Overnight Incubation ◽  
Latex Test

A latex agglutination kit (AUREUS TEST™) for the rapid identification of Staphylococcus aureus was evaluated in this study. The reagent consists of polystyrene latex particles sensitized with rabbit anti-protein A immunoglobulin G and fibrinogen. Due to the respective binding of bacterial protein A with immunoglobulin G and coagulase with fibrinogen, an agglutination reaction occurs within 1 min when the latex is mixed with a suspension of S. aureus. Of 157 S. aureus isolates (138 from foods) and 110 non-S. aureus isolates (58 species belonging to 19 genera), the sensitivity and specificity of the latex test were 100 and 94.4–100%, respectively. The results were comparable to the conventional coagulase test. Therefore, it is proposed that suspicious colonies of S. aureus on Baird-Parker agar medium be subcultured to tryptic soy agar for overnight incubation. Cultures grown on tryptic soy agar are used for latex agglutination test. The latex agglutination test can be finished within minutes and could be used as an alternative rapid procedure of the coagulase test, which needs several hours or even overnight incubation for completion. In addition, all S. aureus isolates grown on Baird-Parker agar also could be correctly identified by the latex reagent.

scholarly journals Efficacy of a Latex Agglutination Test for Rapid Identification of Staphylococcus aureus: Collaborative Study

1996 ◽  
Vol 79 (3) ◽  
pp. 661-670 ◽  
Author(s):  
Tsung C Chang ◽  
Su H Huang ◽  
H Y Chao ◽  
B L Chen ◽  
C Chen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sensitivity And Specificity ◽  
Collaborative Study ◽  
False Negative ◽  
Pairwise Comparison ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Official Method ◽  
Latex Test

Abstract Fifteen laboratories completed a collaborative study comparing the efficacy of a latex agglutination kit (Aureus Test) with that of AOAC Official Method 987.09 (coagulase test for identification of Staphylococcus aureus). Each laboratory analyzed 240 strains of bacteria, including 160 isolates of S. aureus and 80 isolates of other bacteria. Upon receipt of cultures, collaborators subcultured each isolate on both tryptic soy agar (TSA) and Baird-Parker agar medium (BPA) to determine whether the growth medium has any effect on either method. For cultures grown on TSA, the latex test had sensitivity and specificity rates of 99.2 and 97.1 %, respectively, whereas the coagulase test had respective rates of 98.4 and 92.5%. For cultures able to grow on BPA, the latex test had sensitivity and specificity rates of 99.2 and 96.6%, respectively, while the coagulase test had respective rates of 98.3 and 91.3%. By using the McNemar pairwise comparison test of the 2 methods, the falsepositive and false-negative rates of the latex test were significantly lower (p < 0.01) than those of the coagulase test for strains grown either on TSA or BPA. The latex agglutination test for identification of S. aureus isolated from foods has been adopted by AOAC INTERNATIONAL.

Immunochemical qualitative latex agglutination test for pancreatic lipase in serum evaluated for use in diagnosis of acute pancreatitis.

1985 ◽  
Vol 31 (7) ◽  
pp. 1207-1210 ◽  
Author(s):  
J Møller-Petersen ◽  
M Klaerke ◽  
F Dati ◽  
T Toth
Keyword(s):  
Acute Pancreatitis ◽  
Abdominal Pain ◽  
Pancreatic Lipase ◽  
Predictive Value ◽  
Acute Abdominal Pain ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Test Results ◽  
Latex Test

Abstract In 417 patients (213 men, 204 women) consecutively hospitalized with acute abdominal pain we evaluated the clinical usefulness of a latex-agglutination test at admission to screen for concentrations of pancreatic lipase (EC 3.1.1.3) in serum greater than 300 micrograms/L. The diagnoses of acute pancreatitis (in 25 patients, 6%) and other diseases were made without knowledge of the results of the latex test or of quantification of pancreatic lipase in the serum by enzyme immunoassay. In the latex assay, when agglutination was taken as a positive test for acute pancreatitis, we found a diagnostic efficiency of 0.986 (95% confidence limits: 0.971-0.997) for acute pancreatitis. The predictive value of a positive latex test result with respect to acute pancreatitis was 0.807 (0.625-0.926); the predictive value of a negative test was 1.000 (0.991-1.000). Six patients had false-positive test results. No false-negative test results were found by enzyme immunoassay. We conclude that the latex agglutination test is useful as an emergency test for diagnosis of acute pancreatitis in patients with acute abdominal pain; negative results virtually exclude acute pancreatitis.

scholarly journals Cloning and Expression of Leptospiral Immunoglobulin Like B Gene and Use of The Recombinant Antigen for The Diagnosis of Bovine and Caprine Leptospirosis

2020 ◽  
Vol 8 (2) ◽  
pp. 146-153
Author(s):  
Yosef Deneke ◽  
Rajib Deb
Keyword(s):  
Acute Infection ◽  
High Sensitivity ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Cloning And Expression ◽  
Indian Veterinary Research Institute ◽  
Iptg Induction ◽  
E Coli ◽  
Veterinary Research

In the present study recombinant LigB protein is employed in latex agglutination test, which is a cross reacting lipoprotein able to detect acute infection caused by any pathogenic leptospiral serovars. It was employed  for serodiagnosis of leptospirosis. The 46KDa 6X His tagged LigB protein, obtained by IPTG induction of recombinant E. coli M15 cells containing the N-terminal region of LigB gee in PQE30 expression vector, was purified by Ni-NTA affinity chromatography and adsorbed on latex bead surface for performing latex agglutination test against Leptospirosis suspected field sea. Western blot confirmed that rLigB is an immunodominant protein against which antibodies are produced during active infection. A total of 453 field sera, including 432 bovine sera, 18 caprine sera and three sera samples of buffalo bull collected post-mortem following death the animal from Indian Veterinary Research institute (IVRI) were tested using rLigB based LAT. The result showed that 300 sera were tested positive by rLigB based LAT, which were reconfirmed using microscopic agglutination test (MAT). The results from LAT were in concordance with MAT. In conclusion, under laboratory and field conditions, rLigB based LAT is a rapid, pen site, reliable diagnostic tool of high sensitivity and specificity for the detection of Leptospirosis. Int. J. Appl. Sci. Biotechnol. Vol 8(2): 146-153

scholarly journals Comparative evaluation of recombinant LigB based latex agglutination test with microscopic agglutination test for the diagnosis of wildlife leptospirosis

2020 ◽  
Vol 6 (3) ◽  
pp. 440-448
Author(s):  
Yosef Deneke ◽  
Rajib Deb ◽  
SM Lutful Kabir
Keyword(s):  
Comparative Evaluation ◽  
Acute Infection ◽  
High Sensitivity ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Iptg Induction ◽  
E Coli ◽  
Sera Samples

In the present study a comparative evaluation microscopic agglutination test with rLigB protein based latex agglutination test was carried out, which is a cross reacting lipoprotein able to detect acute infection caused by any pathogenic leptospiralserovars. It was employed for serodiagnosis of leptospirosis. The 46 KDa 6X His tagged LigB protein, obtained by IPTG induction of recombinant E. coli M15 cells containing the N-terminal region of LigB gee in PQE30 expression vector, was purified by Ni-NTA affinity chromatography and adsorbed on latex bead surface for performing latex agglutination test against Leptospirosis suspected wildlife field sera. A total of 80 wildlife sera samples were collec ted, including 27 wild feline sera samples (18 tigers, 8 lions, and 1 jaguar) obtained from Chhatbir zoo, Chandigarh, 42 sera samples (8 tigers, 4 lions and 6 leopards, 2 cheethals, 1 black buck, 12 buffaloes and 9 zoo staff) were received from Jodhpur zoo Rajasthan, 8 sera samples (4 tigers, 3 leopards, 1 lion) from Van Vihar National park, Bohpal, Madhya Pradesh and 3 sera samples (2 lions and 1 tiger) received from Biwani Mini zoo, Haryana, India. The result showed that sera were tested positive by rLigB based LAT, which were reconfirmed using microscopic agglutination test (MAT). The results from LAT were in concordance with MAT. In conclusion, rLigB based LAT is a rapid, pen site, reliable diagnostic tool of high sensitivity and specificity, under laboratory and field conditions, for the detection of Leptospirosis. Asian J. Med. Biol. Res. September 2020, 6(3): 440-448

Detection of rotavirus in sewage and drinking water by Latex agglutination test

2019 ◽  
Vol 9 (2) ◽  
pp. p8694
Author(s):  
Muhammad Shoaib ◽  
Kokab Furqan ◽  
Sajjad ur Rahman ◽  
Ahsan Naveed ◽  
Amjad Islam Aqib ◽  
...  
Keyword(s):  
Drinking Water ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test
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