Teknologi Budidaya Tanaman Tomat Melalui Inverted Gardening dan Conventional Gardening Berbasis Pemanfaatan Bakteri Indigenus

ABSTRAK. Inokulan padat Azzofor-wd3 merupakan campuran 16 isolat bakteri indigenus lahan gambut (Rhizobium, Azotobacter, Azospirillum, dan bakteri pelarut fosfat) masing-masing empat isolat digunakan sebagai pupuk hayati untuk meningkatkan produksi tomat dalam inverted dan conventional gardening. Penelitian bertujuan mengetahui peran potensial inokulan padat Azzofor-wd3 sebagai plant growth promoter dalam kondisi lingkungan ekstrim, khususnya pada lahan gambut. Penelitian dilaksanakan di Pusat Penelitian Biologi, LIPI, dari Bulan Januari sampai dengan Desember 2011 Penelitian menggunakan rancangan acak lengkap dengan 28 perlakuan penambahan media pupuk pada media tanam dengan tiga ulangan atau pot. Media dasar ialah gambut (50%) dan tambahan pupuk hayati (50%). Perlakuan tambahan media pupuk mencakup : (1) gambut sebagai kontrol, (2) sekam kotoran ayam, (3) kompos, (4) pasir halus, (5) kapur, (6) Azzofor-wd3, (7) sekam kotoran ayam + pasir halus, (8) sekam kotoran ayam + kapur, (9) sekam kotoran ayam + Azzofor-wd3, (10) kompos + pasir halus, (11) kompos + kapur, (12) kompos + Azzofor-wd3, (13) pasir halus + kapur, (14) pasir halus + Azzofor-wd3, (15) kapur + Azzofor-wd3, (16) sekam kotoran ayam + kompos + pasir halus, (17) sekam kotoran ayam + kompos + kapur, (18) sekam kotoran ayam + kompos + Azzofor-wd3, (19) sekam kotoran ayam + pasir halus + kapur, (20) sekam kotoran ayam + pasir halus + Azzofor-wd3, (21) sekam kotoran ayam + kapur + Azzofor-wd3, (22) kompos + pasir halus + kapur, (23) kompos + kapur + Azzofor-wd3, (24) pasir halus + kapur + Azzofor-wd3, (25) sekam kotoran ayam + kompos + pasir halus, (26) sekam kotoran ayam + kompos + pasir halus + Azzofor-wd3, (27) kompos + pasir halus + kapur + Azzofor-wd3, dan (28) sekam kotoran ayam + kompos + pasir halus + kapur + Azzofor-wd3. Hasil penelitian menunjukkan bahwa produksi tomat tertinggi setelah 3 bulan ialah pada perlakukan media gambut + sekam kotoran ayam + kompos + pasir halus + kapur + Azzofor-wd3 pada inverted dan conventional gardening, masing-masing sebesar 63,9 dan 65,9 g/pot. Terdapat perbedaan pengaruh perlakukan yang nyata antara inverted dan conventional gardening dalam hal P-tersedia, populasi bakteri, dan aktivitas PME-ase. Namun demikian, tidak ada pengaruh perlakuan yang nyata terhadap produksi tomat antara inverted dan conventional gardening. Hasil penelitian ini menunjukkan bahwa Azzofor-wd3 merupakan bakteri pendorong pertumbuhan tanaman yang potensial untuk tanaman tomat yang dibudidayakan pada lahan gambut. Aplikasi jenis bakteri tersebut sangat bermanfaat dalam pengayaan tanah gambut untuk pembudidayaan tanaman sayuran. ABSTRACT. Widawati, S, Sudiana, IM, Sukara, E, and Muharam, A 2012. The Technology of Tomato Plant Cultivation Through Inverted and Conventional Gardening Based on Utilization of Indigenous Bacteria. Azzofor-wd3 is a solid inoculant consisted of 16 peat indigenous bacteria isolates i.e. Rhizobium, Azotobacter, Azospirillum, and PSB four isolates respectively were used as biofertilizers to stimulate tomato production on inverted and conventional gardening. An experiment was conducted at the Research Center for Biology, Indonesian Institute of Sciences from January until December 2011. The research was aimed to determine the potential role of Azzofor-wd3 solid inoculant as a plant growth promoter in extremely environmental conditions. The treatments of growth media mixture were arranged in a completely randomized design with three replications. The based media was peat for 50% of mixture. The treatments were the addition of biofertilizers with the same volume of the based media (50%). The treatments were (1) peat only as the control, (2) chicken dunk, (3) compost, (4) fine sand, (5) lime, (6) Azzofor-wd3 inoculant, (7) chicken dunk + fine sand, (8) chicken dunk + lime, (9) chicken dunk + Azzofor-wd3, (10) compost + fine sand, (11) compost + lime, (12) compost + Azzofor-wd3, (13) fine sand + lime, (14) fine sand + Azzofor-wd3, (15) lime + Azzofor-wd3, (16) chicken dunk + compost + fine sand, (17) chicken dunk + compost + lime, (18) chicken dunk + compost + Azzofor-wd3, (19) chicken dunk + fine sand + lime, (20) chicken dunk + fine sand + Azzofor-wd3, (21) chicken dunk + lime + Azzofor-wd3, (22) compost + fine sand + lime, (23) compost + lime + Azzofor-wd3, (24) fine sand + lime + Azzofor-wd3, (25) Chicken dunk + compost + fine sand, (26) chicken dunk + compost + fine sand + Azzofor-wd3, (27) compost + fine sand + lime + Azzofor-wd3, and (28) chicken dunk + compost + fine sand + lime + Azzofor-wd3. The results showed that the highest production of tomato in inverted gardening was 63.9 g/pot and in conventional gardening was 65.9 g/pot produced by the plants grown on peat + chicken dunk + compost + sand + lime + Azzofor wd3 inoculant, 3 months after planting. There was significant difference of available-P, bacterial population, and PME-ase activity in inverted and conventional gardening before and after fertilization, whereas there was no significant difference of tomato yield between inverted and convensional gardening. It can be concluded that Azzofor-wd3 is potential as a plant growth promoting bacteria for tomato plants grown in peat soil. The application of the bacteria is very helpful to enrich peat soil for growing vegetable crops.

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Variovorax sp. Has an Optimum Cell Density to Fully Function as a Plant Growth Promoter

Microorganisms ◽

10.3390/microorganisms7030082 ◽

2019 ◽

Vol 7 (3) ◽

pp. 82 ◽

Cited By ~ 1

Author(s):

Oyungerel Natsagdorj ◽

Hisayo Sakamoto ◽

Dennis Santiago ◽

Christine Santiago ◽

Yoshitake Orikasa ◽

...

Keyword(s):

Plant Growth ◽

Cell Density ◽

Growth Promotion ◽

Growth Promoter ◽

Plant Growth Promoting Bacteria ◽

Biochemical Tests ◽

Green Pepper ◽

Plant Growth Promoter ◽

Plant Growth Promoting ◽

Growth Promoting

Utilization of plant growth-promoting bacteria colonizing roots is environmentally friendly technology instead of using chemicals in agriculture, and understanding of the effects of their colonization modes in promoting plant growth is important for sustainable agriculture. We herein screened the six potential plant growth-promoting bacteria isolated from Beta vulgaris L. (Rhizobium sp. HRRK 005, Polaromonas sp. HRRK 103, Variovorax sp. HRRK 170, Mesorhizobium sp. HRRK 190, Streptomyces sp. HRTK 192, and Novosphingobium sp. HRRK 193) using a series of biochemical tests. Among all strains screened, HRRK 170 had the highest potential for plant growth promotion, given its ability to produce plant growth substances and enzymes such as siderophores and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, respectively, concomitantly with active growth in a wider range of temperatures (10–30 °C) and pH (5.0–10.0). HRRK 170 colonized either as spots or widely on the root surface of all vegetable seedlings tested, but significant growth promotion occurred only in two vegetables (Chinese cabbage and green pepper) within a certain cell density range localized in the plant roots. The results indicate that HRRK 170 could function as a plant growth promoter, but has an optimum cell density for efficient use.

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Potential of endophytic fungus Aspergillus terreus as potent plant growth promoter

Pakistan Journal of Botany ◽

10.30848/pjb2020-3(44) ◽

2019 ◽

Vol 52 (3) ◽

Cited By ~ 1

Author(s):

Arooj Javed ◽

Azhar Hussain Shah ◽

Anwar Hussain ◽

Zabta Khan Shinwari ◽

Seema Ali Khan ◽

...

Keyword(s):

Plant Growth ◽

Endophytic Fungus ◽

Aspergillus Terreus ◽

Growth Promoter ◽

Plant Growth Promoter

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Plant growth promoter produced by Trichoderma virens and its effect on mungbean (Vigna radiata (L.) Wilczek) seedling

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/803/1/012013 ◽

2021 ◽

Vol 803 (1) ◽

pp. 012013

Author(s):

A Inayati ◽

L Setyowati ◽

L Q Aini ◽

E Yusnawan

Keyword(s):

Plant Growth ◽

Vigna Radiata ◽

Growth Promoter ◽

Trichoderma Virens ◽

Plant Growth Promoter

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The potential of indigenous rhizobacteria from areca nut rhizosphere in South Konawe regency as a plant growth promoter

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/782/3/032029 ◽

2021 ◽

Vol 782 (3) ◽

pp. 032029

Author(s):

G A K Sutariati ◽

Muhidin ◽

A Khaeruni ◽

N M Rahni ◽

T C Rakian ◽

...

Keyword(s):

Plant Growth ◽

Growth Promoter ◽

Areca Nut ◽

Plant Growth Promoter

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Uji Laboratorium Azospirillum sp. yang Diisolasi dari Beberapa Ekosistem

Jurnal Hortikultura ◽

10.21082/jhort.v22n3.2012.p258-267 ◽

2013 ◽

Vol 22 (3) ◽

pp. 258

Author(s):

S Widawati ◽

Agus Muharam

Keyword(s):

Acetic Acid ◽

Plant Growth ◽

Laboratory Test ◽

Indole Acetic Acid ◽

Bacterial Species ◽

Coastal Ecosystem ◽

Laboratory Research ◽

Growth Promoter ◽

Plant Growth Promoter ◽

Qualitative Test

ABSTRAK. Beberapa mikrob yang bersifat nonpatogenik dan nonsimbiotik yang efektif menambat nitrogen dari udara serta mampu melarutkan P terikat pada Ca, Al, dan Fe dalam tanah, dapat hidup dalam berbagai ekosistem di alam. Sebagian bakteri tersebut dapat diisolasi dari daerah perakaran tanaman hortikultura. Penelitian bertujuan mengetahui peran Azospirillum sp. yang potensial sebagai pendorong pertumbuhan tanaman pada ekosistem pantai dan kondisi lingkungan yang ekstrim. Pengujian terhadap isolat bakteri yang dikumpulkan dari berbagai kondisi ekosistem dilaksanakan di Laboratorium Ekofisiologi, Pusat Penelitian Biologi, LIPI, Bogor dari Bulan Januari sampai dengan Desember 2011. Sebanyak 34 isolat Azospirillum sp. diuji dengan berbagai metode, yaitu (1) uji kualitatif kemampuan isolat Azospirillum sp. dalam menambat (fiksasi) nitrogen dan kemampuan hidup pada media Okon padat yang mengandung NaCl, (2) uji kualitatif kemampuan isolat Azospirillum sp. dalam melarutkan P terikat pada Ca3(PO4)2 dalam media Pikovskaya padat dan indeks efisiensi pelarutan fosfat, (3) uji kualitatif kemampuan isolat Azospirillum sp. dalam melarutkan P terikat pada media Pikovskaya cair dan aktivitas enzim PME-ase asam dan basa, serta kondisi pH selama inkubasi 7 hari pada kultur murni (pH asal= 7), dan (4) analisis kemampuan Azospirillum sp. dalam memproduksi indole acetic acid (IAA). Hasil penelitian menunjukkan bahwa: (1) semua isolat bakteri yang diuji mampu menambat nitrogen dalam media Okon padat, (2) isolat B2, B4, B6, B12, B14, PS2, dan FR13 mampu melarutkan P dari Ca3(PO4)2 dalam medium Pikovskaya padat dengan masing-masing indeks efisiensi pelarutan sebesar 120, 160, 140, 100, 110, 120, dan 100, (3) isolat B1, B2, B3, B4, B6, B14, B17, PS1, PS2, PS3, FR1, FR5, FR7, FR8, FR10, FR12, dan FR13 mampu tumbuh dalam medium Okon dengan kandungan NaCl sebesar 0, 2, 4, atau 6%, (4) konsentrasi tertinggi P terlarut dihasilkan oleh isolat B4 (5,80 mg/l), B6 (5,84 mg/l), dan PS2 (5,45 mg/l) dengan PME-ase sebesar 0,58 u m/l, 0,58 u m/l, 0,57 u m/l (asam), 0,52 mg/l, 0,50 mg/l, 0,48 mg/l (basa), dan dengan pH : 4,20, 4,30, dan 4,22, dan (5) isolat B4 dan B6 yang diisolasi dari pertanaman padi di pantai Rambut Siwi, Bali, mampu memproduksi IAA tertinggi, yaitu masing-masing sebesar 0,6749 dan 0,4694 ppm pada hari pertama setelah perlakuan. Berdasarkan hasil penelitian ini terbukti bahwa isolat Azospirillum sp. berpotensi sebagai plant growth promoter untuk ekosistem di daerah pesisir atau pantai. Bakteri tersebut sangat penting untuk pengkayaan nutrisi pada lahan di daerah dataran rendah atau pantai dalam rangka pengembangan tanaman termasuk komoditas hortikultura. ABSTRACT. Widawati, S and Muharam, A 2012. The Laboratory Test of Azospirillum sp. Isolated from Several Ecosystems. Microbes that are nonpathogenic and nonsymbiotic bacteria which are effectively fixed up nitrogen from air, and are able to dissolve phosphated bounded on Ca, Al, and Fe in soil, are able to growth in different ecosystems in nature. Some of the bacterial species can be isolated from rizosphere of horticultural crops. The research was aimed to determine the potential role of Azospirillum sp. as a plant growth promoter in coastal ecosystem and extremely environmental conditions. The laboratory test of Azospirillum sp. isolated from several ecosystems was carried out in the Ecophysiology Laboratory, Research Center for Biology, Indonesian Institute of Sciences, Bogor from January until December 2011. Thirty-four isolates of Azospirillum sp. (B1 to B17;PS1 to PS3; FR1 to FR 14) were investigated with some methods i.e. (1) the qualitative test of the capability of Azospirillum sp. to fix up nitrogen in solid Okon medium containing NaCl, (2) the qualitative test of the capability of Azospirillum sp. in dissolving bounded P in solid Pikovskaya medium and phosphate dissolution efficiency index, (3) the qualitative test of the capability of Azospirillum sp. in dissolving bounded P in liquid Pikovskaya medium and the activity of acid and base PME-ase, and pH condition after 7 days incubation in pure media, and (4) analysis of the capability of Azospirillum sp. in producing indole acetic acid (IAA). The results pointed out that : (1) all tested isolates of Azospirillum sp. were capable to fix up nitrogen in solid Okon medium, (2) isolates of B2, B4, B6, B12, B14, PS2, and FR13 were capable to solubilize P on Ca3(PO4)2 in solid Pikovskaya medium with its efficiency of 120, 160, 140, 100, 110, 120, and 100, respectively, (3) isolates of B1, B2, B3, B4, B6, B14, B17, PS1, PS2, PS3, FR1, FR5, FR7, FR8, FR10, FR12, and FR13 were able to grow in Okon medium with 0, 2, 4, or 6% of NaCl doses, (4) the highest concentrations of solubilized P was resulted by isolates B4 (5.80 mg/l), B6 (5.84 mg/l), and PS2 (5.45 mg/l) with PME-ase i.e. 0.58 u m/l, 0.58 u m/l, 0.57 u m/l (acid), 0.52 mg/l, 0.50 mg/l, 0.48 mg/l (base), and with pH : 4.20, 4.30, and 4.22, and (5) isolates of B4 and B6 isolated from rice field at Rambut Siwi beach, Bali, were capable to produce highest IAA hormone i.e. 0.6749 and 0.4694 ppm respectively on the first day after the treatment. Based on the result of this experiment it can be concluded that Azospirillum sp. is a potential plant growth promoting Rhizobacteria for coastal ecosystem. The bacterial species is very important to enrich coastal areas for crop cultivation, including horticulture.

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