scholarly journals KANDUNGAN FENOL DAN LIGNIN TANAMAN NILAM HIBRIDA (Pogostemon sp.) HASIL FUSI PROTOPLAS

2020 ◽  
Vol 7 (4) ◽  
pp. 104
Author(s):  
YANG NURYANI ◽  
IKA MUSTIKA ◽  
CHEPPY SYUKUR
Keyword(s):  
High Performance Liquid Chromatography ◽  
Protoplast Fusion ◽  
High Performance ◽  
Somatic Hybrids ◽  
Lignin Content ◽  
Food Crop ◽  
Phenol Content ◽  
Pogostemon Cablin ◽  
Pratylenchus Brachyurus ◽  
Crop Biotechnology

<p><strong>Evaluation of phenols and lignin in the root of hybrid patchouli (Pogostemon cablin Benth.) front protoplast fusion</strong></p><p>One of the problems faced in patchouli production is nematode infection (Pratylenchus brachyurus). Improvement of the plant resistance to nematode in patchouli is dificult 10 be conducted through conventional method as the plant never llowcrs. One of the methods of improving (he plain resistance is by using protoplast fusion of Aceh patchouli (Pogostemon cablin, cv Sidikalang ) and Java patchouli (/' heyneanus. cv (iirilaya). It has been found thai Ihe plain resistance lo nematode is closely related to phenols and lignin content of the root Therefore. Ihe level of resistance of different clones of somatic hybrids lo nematode was approached by evaluating ihe content of phenols and lignin of the roots The study was conducted al the laboratory of the Research Institute for food Crop Biotechnology, Bogor in November 2000. Phenol content was determined with high performance liquid Chromatography (IIPI.C). while lignin content was determined with Klasou method. Result showed that Java patchouli (Girilaya) contained phenols and lignin respectively 76.53 and 21 900 pp. higher than those of Aceh patchouli (Sidikalang and Tapak Tuan) respectively 38 2-81 45 and 8 000-7 200 ppm. Phenol and lignin content in the somatic hybrids were dilferenl in Ihe respective pairs. In the fusion of Girilaya x Sidikalang. all clones contained phenol lower than those of Iheir parents, some clones (29%) contained lignin higher than Sidikalang but lower than (iirilaya. and the rest (71%) contained lignin lower than the two parents. Meanwhile, from Ihe fusion of Girilaya, Tapak Tuan. I clone (4%) contained phenols higher than those of (he two parents (97 ppm). 39% contained phenols higher than Tapak Tuan but lower than Girilaya (38.75-60.12 ppm) and the rests (60%) contained phenol lower than the two parents. Furthermore, the lignin content, of 78% somatic hybrids was lower than Girilaya but higher than Tapak Tuan, but the rest (22%) was lower than the two parents. Based on the distribution of phenols and lignin content. Ihe somatic hybrids can be categorized in three group The irst groups (5 clones) contained high phenols (higher than the average). Ihe second group (9 clones) contained high lignin. and Ihe third (I clone) contained high phenol and lignin.</p>

Superficial CO2 Extraction Oil from Schisandra and the Component Differences Based on Regional

2014 ◽  
Vol 675-677 ◽  
pp. 1638-1641
Author(s):  
Zhen Yu Zhao ◽  
Cheng Yi Zhang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Solvent Extraction ◽  
Organic Solvent ◽  
Quality Index ◽  
High Performance ◽  
Lignin Content ◽  
Oil Yield ◽  
Extraction Temperature ◽  
Schisandra Chinensis ◽  
Organic Solvent Extraction

Schisandra Chinensis Baill from four places were extracted with superficial CO2 fluid extraction (SCDE) to explore the component differences based on regional. The condition of SCDE for Schisandra oil are: 18 MPa extraction pressure 6 Mpa , extraction temperature 40 °C and separate temperature for 90 minutes. The Schisandra oil were analyzed by high performance liquid chromatography (HPLC), using the oil yield and the lignin content as the quality index of the Schisandra. The results will be compared with the results of organic solvent extraction. For the extraction of Schisandra oil, SCDE is superior to the organic solvent extraction. The component differences of the Schisandra oil from four different places are obvious, and the oil yield and the lignin content of Schisandra from Changbai Mountain in Jilin Province is the highest.

888: Metabolomic Urine Samples Profiling Using High Performance Liquid Chromatography Coupled with Mass Spectrometry to Detect Bladder Cancer

2007 ◽  
Vol 177 (4S) ◽  
pp. 295-295
Author(s):  
Michael Mullerad ◽  
Haleem J. Issaq ◽  
Alexander Kravtsov ◽  
Timothy Waybright ◽  
Brian Luke ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bladder Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Urine Samples

Quantification of Vitamin A in Fortified Rapeseed, Groundnut and Soya Oils Using a Simple Portable Device: Comparison to High Performance Liquid Chromatography

2013 ◽  
Vol 83 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Cécile Renaud ◽  
Jacques Berger ◽  
Arnaud Laillou ◽  
Sylvie Avallone
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Developed Countries ◽  
Portable Device ◽  
Least Developed Countries ◽  
Control Food ◽  
Assay Precision

Vitamin A deficiency is still one of the major public health problems in least developed countries. Fortification of vegetable oils is a strategy implemented worldwide to prevent this deficiency. For a fortification program to be effective, regular monitoring is necessary to control food quality in the producing units. The reference methods for vitamin A quantification are expensive and time-consuming. A rapid method should be useful for regular assessment of vitamin A in the oil industry. A portable device was compared to high-performance liquid chromatography (HPLC) for three plant oils (rapeseed, groundnut, and soya). The device presented a good linearity from 3 to 30 mg retinol equivalents per kg (mg RE.kg- 1). Its limits of detection and quantification were 3 mg RE.kg- 1 for groundnut and rapeseed oils and 4 mg RE.kg- 1 for soya oil. The intra-assay precision ranged from 1.48 % to 3.98 %, considered satisfactory. Accuracy estimated by the root mean squares error ranged from 3.99 to 5.49 and revealed a lower precision than HPLC (0.4 to 2.25). Although it offers less precision than HPLC, the device estimates quickly the vitamin A content of the tested oils from 3 or 4 to 15 mg RE.kg- 1.

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