Quantification of Vitamin A in Fortified Rapeseed, Groundnut and Soya Oils Using a Simple Portable Device: Comparison to High Performance Liquid Chromatography

2013 ◽  
Vol 83 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Cécile Renaud ◽  
Jacques Berger ◽  
Arnaud Laillou ◽  
Sylvie Avallone
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Developed Countries ◽  
Portable Device ◽  
Least Developed Countries ◽  
Control Food ◽  
Assay Precision

Vitamin A deficiency is still one of the major public health problems in least developed countries. Fortification of vegetable oils is a strategy implemented worldwide to prevent this deficiency. For a fortification program to be effective, regular monitoring is necessary to control food quality in the producing units. The reference methods for vitamin A quantification are expensive and time-consuming. A rapid method should be useful for regular assessment of vitamin A in the oil industry. A portable device was compared to high-performance liquid chromatography (HPLC) for three plant oils (rapeseed, groundnut, and soya). The device presented a good linearity from 3 to 30 mg retinol equivalents per kg (mg RE.kg- 1). Its limits of detection and quantification were 3 mg RE.kg- 1 for groundnut and rapeseed oils and 4 mg RE.kg- 1 for soya oil. The intra-assay precision ranged from 1.48 % to 3.98 %, considered satisfactory. Accuracy estimated by the root mean squares error ranged from 3.99 to 5.49 and revealed a lower precision than HPLC (0.4 to 2.25). Although it offers less precision than HPLC, the device estimates quickly the vitamin A content of the tested oils from 3 or 4 to 15 mg RE.kg- 1.

Quantification of Vitamin A in Palm Oil Using a Fast and Simple Portable Device: Method Validation and Comparison to High-Performance Liquid Chromatography

2011 ◽  
Vol 81 (5) ◽  
pp. 335-342 ◽  
Author(s):  
Fabian Rohner ◽  
Simone K. Frey ◽  
Ralf Mothes ◽  
Andrea Hurtienne ◽  
Simone Hartong ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Palm Oil ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Public Health Problem ◽  
Determination Limit ◽  
New Device ◽  
Refined Palm Oil

Vitamin A deficiency continues to be a global public health problem. Fortification of oil with vitamin A is considered a cost-effective, feasible strategy to prevent this problem but quality control poses a challenge to program implementation. To overcome this, we have validated a newly developed device that quantitatively measures the content of retinyl palmitate in refined palm oil, is simple to use, and yields immediate results.Linearity of analysis ranged from 2.5 - 30 mg retinol equivalents (RE)/ kg of palm oil, with 2.5 mg RE/kg being the determination limit; inter- and intra-assay precision ranged from 1.4 - 7.1 %. Comparison with a high-performance liquid chromatography method showed high agreement between the methods (R2 = 0.92; Limits of Agreement: -1.24 mg to 2.53 mg RE/kg), and further comparisons illustrate that the new device is useful in low-resource settings. This device offers a field- and user-friendly solution to quantifying the vitamin A content in refined palm oil.

Total Carotenoids Content and Assessment of Carotene Profile by High- Performance Liquid Chromatography in Selected Vegetables of Bangladesh with Special Reference to Some Unconventional Varieties

2020 ◽  
Vol 7 (1) ◽  
pp. 24-31
Author(s):  
Samia Sams ◽  
Mohammad Khairul Alam ◽  
Monira Ahsan ◽  
Sheikh Nazrul Islam
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Health Benefit ◽  
Food Composition ◽  
Leafy Vegetables ◽  
Immune Functions ◽  
Total Carotenoids

Background: Carotenoids are natural plant pigments and precursors of vitamin A, which provide a health benefit, protect against chronic degenerative diseases, and contribute to immune functions. Utilization and identification of foods with a high content of carotenoids received greater attention nowadays. Objective: The present study was aimed at evaluating total carotenoids content (TCC) and carotene composition of unconventional leafy vegetables growing in Bangladesh. Materials and Methods: The samples were collected from different locations of Bangladesh and mixed together to ensure sample representativeness. Acetone–petroleum ether extraction followed by spectrophotometric measurement was utilized for quantification of TCC. High-performance liquid chromatography (HPLC- DAD) was used to separate and quantify carotene components. Results and Discussion: The TCC values ranged from 129.38 μg/100 g edible portion (EP) in Helencha to 12803.31 μg/100 g EP in Roktodrone. The study findings also revealed that the selected plant samples exhibited high content of β-carotene ranging from 120.99 μg/100 g EP in Parul to 11301.16 μg/100 g EP in Roktodrone. It was also observed that most of the vegetables analyzed did not have α-carotene, lycopene, β-cryptoxanthin or it was in such a small quantity that could not be detected with HPLC. Conclusion: It can be suggested that regular intake of these vegetables may provide pro-vitamin A. In order to address the vitamin A deficiency and to maintain biodiversity, the analysis of carotenoids composition of these unconventional plant foods is to be initiated and make it available to the mass population. It would also fill up the data gap in the existing food composition table of Bangladesh.

Tingginya tingkat konsumsi minyak goreng oleh Masyarakat Indonesia menjadikan minyak goreng digunakan sebagai food vehiclepada program fortifikasi vitamin A.Program tersebut dilakukan untuk mengatasi Kekurangan vitamin A (KVA). KVAsangat mempengaruhi kela

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
LILY RESTUSARI ◽  
SRI WIDIA NINGSIH ◽  
YULIANA ARSIL
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance

Tingginya tingkat konsumsi minyak goreng oleh Masyarakat Indonesia menjadikan minyak goreng digunakan sebagai food vehiclepada program fortifikasi vitamin A.Program tersebut dilakukan untuk mengatasi Kekurangan vitamin A (KVA). KVAsangat mempengaruhi kelangsungan hidup anak dan standar upaya penyelamatan proses kehamilan dan persalinan. Namun pemanasan yang terjadi selama proses penggorenganpada minyak dapatmerusak jumlah vitamin A yang difortifikasi. Tujuan penelitian ini untuk membandingkan nilai retensi vitamin A di dalam kentang yang digoreng dengan menggunakan minyak goreng curah dan minyak goreng kemasan fortifikasi dengan pengaruh suhu dan waktu penggorengan. Penelitian ini menggunakan metode eksperimental disain rancangan acak lengkap (RAL) dengan dua faktor, suhu (140dan 170oC) dan waktu penggorengan (10 dan 15 menit) tanpa pengulangan.Sampel penelitian yang digunakan adalah kentang beku, minyak goreng curah dan minyak goreng kemasan fortifikasi.Nilai retensi vitamin A kentang dan minyak goreng diukur dengan menggunakan metode High Performance Liquid Chromatography (HPLC).Analisis perbedaan dan interaksi variabel diolah dengan ANOVA dua arah. Berdasarkan luas area puncak vitamin A pada kromatogram sebelum dan setelah penggorengan, ditemukan kecenderungan penurunan persentase retensi vitamin A dari minyak goreng kemasan fortifikasi dibandingkan minyak goreng curah.Nilai retensi vitamin A pada kentang yang digoreng dengan minyak goreng curah adalah 217-251%,sedangkan retensivitamin A kentang yang digoreng dengan minyak goreng kemasan fortifikasi adalah 48-131%. Analisis ANOVA dua arah tanpa pengulangan untuk persen retensi vitamin A menunjukkan bahwa (p<0,05) variabel jenis minyak goreng berpengaruh secara nyata terhadap retensi vitamin A. Retensi vitamin A pada kentang goreng yang digoreng dengan minyak goreng curah jauh lebih tinggi dibanding minyak goreng kemasan fortifikasi

Analysis of 8-methoxypsoralen by high-performance liquid chromatography

1990 ◽  
Vol 36 (11) ◽  
pp. 1956-1957 ◽  
Author(s):  
C H Ketchum ◽  
C A Robinson ◽  
S T Huang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Lower Limit ◽  
High Performance ◽  
Limit Of Detection ◽  
Extraction Recovery ◽  
Rapid Procedure ◽  
Standard Curve ◽  
Assay Precision ◽  
Interassay Precision

Abstract We report a simple and rapid procedure for assaying 8-methoxypsoralen (8-MOP) in plasma by high-performance liquid chromatography (HPLC). The standard curve for the assay is linear for 8-MOP from 15 to 500 micrograms/L (y = 0.002x-0.01, r = 0.99) with a lower limit of detection of 1.5 micrograms/L. Intra-assay precision (CV) was 6.0% at the 100 micrograms/L concentration and 10.0% at 50 micrograms/L (n = 30 each). Interassay precision was 6.4% at 100 micrograms/L and 7.0% at 50 micrograms/L (n = 50 each). Extraction recovery of 8-MOP was 98%. Common antiarrhythmics, sedatives, and hypnotics were found not to interfere.

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