scholarly journals Spectrophotometric determination of Metronidazole and Metronidazole benzoate via first and Second Derivative order spectroscopy

2017 ◽  
Vol 14 (2) ◽  
pp. 320-327
Author(s):  
Baghdad Science Journal
Keyword(s):  
Spectrophotometric Determination ◽  
Base Line ◽  
Second Derivative ◽  
Derivative Spectrum ◽  
Spectrophotometric Methods ◽  
Concentration Peak ◽  
Metronidazole Benzoate ◽  
Derivative Order

A New Spectrophotometric Methods are improved for determination Metronidazole (MTZ) and Metronidazolebenzoate (MTZB) depending on1STand 2nd derivative spectrum of the two drugs by using ethanol as a solvent. Many techniques were proportionated with concentration (peak high to base line, peak to peak and peak area). The linearity of the methodsranged between(1-25µg.ml-1) is obtained. The results were precise and accurate throw RSD% were between (0.041-0.751%) and (0.0331-0.452%), Rec% values between (97.78, 101.87%) and (98.033-102.39%) while the LOD between (0.051-0.231 µg.ml-1) and (0.074-1.04 µg.ml-1) and LOQ between (0.170-0.770µg.ml-1) and (0.074-0.313 µg.ml-1) of (MTZ) and of (MTZB) respectively. These Methods were successfully applied to determination of (MTZ) and (MTZB) in the pharmaceutical preparatio; ns

scholarly journals VALIDATED SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF CINNARIZINE IN BINARY MIXTURE WITH PARACETAMOL IN BULK AND TABLETS

2021 ◽  
pp. 161-166
Author(s):  
AHMED M AL-GHANI ◽  
ANES AM THABET
Keyword(s):  
Quality Control ◽  
Second Derivative ◽  
Derivative Spectrum ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Derivative Method ◽  
Analytical Tools ◽  
Second Derivative Method

Objectives: The aim of this work was to develop and validate new, simple, accurate, and selective spectrophotometric methods (derivative and derivative ratio spectrophotometric methods) for the determination of these drugs. These methods can be used as analytical tools in routine examination in quality control laboratories. Methods: The first method was derivative method in which the first derivative method (1D) for determination of PCM and the second derivative method (2D) for determination of CIN. The second method was the first derivative ratio spectrophotometric method (1DD) for determination of CIN and PCM. Results: In first method, the first derivative spectrum (1D) of PCM where PCM was determined by measuring the amplitude of the valley at 235 nm while CIN showed zero crossing spectrum, and the second derivative spectrum (2D) of CIN where CIN was determined by measuring the amplitude of the peak at 287.5 nm while PCM showed a zero value. In the second method, the first derivative ratio spectrophotometry (1DD) for CIN and PCM determination, where the amplitude at 290 and 291 nm, was selected for the determination of CIN and PCM, respectively. Conclusions: The developed methods were applied for the determination of the cited drugs in tablets containing binary drug mixture. The methods are simple and precise and can be used for routine analysis of the labeled drugs in combined dosage forms in quality control laboratories.

Simultaneous Second Derivative Spectrophotometric Determination of Manganese and Copper

1992 ◽  
Vol 25 (8) ◽  
pp. 1581-1593 ◽  
Author(s):  
A. R. Fernández-Alba ◽  
J. L. Martínez-Vidal ◽  
P. Aguilera ◽  
F. Freniche ◽  
A. Agüera
Keyword(s):  
Spectrophotometric Determination ◽  
Second Derivative

DC Polarographic and Spectrophotometric Determination of Ampicillin Capsules

1980 ◽  
Vol 63 (5) ◽  
pp. 1049-1051
Author(s):  
Juan A Squella ◽  
Luis J Nunez-Vergara ◽  
Maximo Aros
Keyword(s):  
Absorption Band ◽  
Spectrophotometric Determination ◽  
Degradation Product ◽  
Uv Absorption ◽  
Acidic Hydrolysis ◽  
Average Recovery ◽  
Polarographic Wave ◽  
Spectrophotometric Methods ◽  
Hydrolysis Of

Abstract Polarographic and spectrophotometric methods are proposed for the determination of ampicillin in capsules. Acidic hydrolysis of ampicillin with 1% HCHO in 0.3N HCl yields a degradation product identified as 2-hydroxy-3-phenyl-6-methylpyrazine. This compound has a well defined UV absorption band at 380 nm and a polarographic wave at –0.55 V vs SCE, which can be used for analytical purposes. Individual capsule assays, composite assays, and recovery studies are described. The average recovery values and standard deviations (SD) for UV and polarographic determinations were 99.20% (SD 0.95) and 100.85% (SD 1.09), respectively

Different Spectrophotometric and Chromatographic Methods for Determination of Mepivacaine and Its Toxic Impurity

2017 ◽  
Vol 100 (5) ◽  
pp. 1392-1399 ◽  
Author(s):  
Nada S Abdelwahab ◽  
Nehal F Fared ◽  
Mohamed Elagawany ◽  
Esraa H Abdelmomen
Keyword(s):  
Particle Size ◽  
Stationary Phase ◽  
Second Derivative ◽  
Spectrophotometric Measurement ◽  
Spectrophotometric Methods ◽  
Derivative Method ◽  
Tlc Plates ◽  
Second Derivative Method ◽  
Developing System

Abstract Stability-indicating spectrophotometric, TLC-densitometric, and ultra-performance LC (UPLC) methods were developed for the determination of mepivacaine HCl (MEP) in the presence of its toxic impurity, 2,6-dimethylanaline (DMA). Different spectrophotometric methods were developed for the determination of MEP and DMA. In a dual-wavelength method combined with direct spectrophotometric measurement, the absorbancedifference between 221.4 and 240 nm was used for MEPmeasurements, whereas the absorbance at 283 nm was used for measuring DMA in the binary mixture. In the second-derivative method, amplitudes at 272.2 and 232.6 nm were recorded and used for the determination of MEP and DMA, respectively. The developed TLC-densitometric method depended on chromatographic separation using silica gel 60 F254 TLC plates as a stationary phase and methanol–water–acetic acid (9 + 1 + 0.1, v/v/v) as a developing system, with UV scanning at 230 nm. The developed UPLC method depended on separation using a C18 column (250 × 4.6 mm id, 5 μm particle size) as a stationary phase and acetonitrile–water (40 + 60, v/v; pH 4 with phosphoric acid) as a mobilephase at a flow rate of 0.4 mL/min, with UV detection at 215 nm. The chromatographic run time was approximately 1 min. The proposed methods were validated with respect to International Conference on Harmonization guidelines regarding precision, accuracy, ruggedness, robustness, and specificity.

scholarly journals Use of N, N-diethyl-p-phenylenediamine sulphate for the spectrophotometric determination of some phenolic and amine drugs

2010 ◽  
Vol 60 (2) ◽  
pp. 217-227 ◽  
Author(s):  
Padmarajaiah Nagaraja ◽  
Ashwinee Shrestha ◽  
Anantharaman Shivakumar ◽  
Avinash Gowda
Keyword(s):  
Spectrophotometric Determination ◽  
Correlation Coefficients ◽  
Dosage Forms ◽  
Pharmaceutical Dosage Forms ◽  
Variable Parameters ◽  
Spectrophotometric Methods ◽  
Linear Relationships ◽  
Colored Product ◽  
And Performance

Use ofN, N-diethyl-p-phenylenediamine sulphate for the spectrophotometric determination of some phenolic and amine drugsSpectrophotometric methods are proposed for the determination of drugs containing a phenol group [salbutamol sulphate (SLB), ritodrine hydrochloride (RTD), isoxsuprine hydrochloride (IXP)] and drugs containing an aromatic amine group [dapsone hydrochloride (DAP), sulfamethoxazole (SFM), and sulfadiazine (SFD)] in pharmaceutical dosage forms. The methods are based on coupling ofN, N-diethyl-p-phenylenediamine sulphate with the drugs in the presence of KIO4to give a green colored product (λmaxat 670 nm) and a red colored product (λmaxat 550 nm), respectively. Linear relationships with good correlation coefficients (0.9986-0.9996) were found between absorbance and the corresponding concentration of drugs in the range 1-7, 2-22, 1-17, 1.5-12, 2-25, and 2-21 μg mL-1for SLB, RTD, IXP, DAP, SFM and SFD, respectively. Variable parameters such as temperature, reaction time and concentration of the reactants have been analyzed and optimized. The RSD of intra-day and inter-day studies was in the range of 0.2-1.0 and 0.4-1.0%, respectively. No interference was observed from common pharmaceutical adjuvants. The reliability and performance of the proposed methods was validated statistically; the percentage recovery ranged from 99.5 ± 0.1 to 99.9 ± 0.3%. Limits of detection were 0.14, 0.21, 0.51, 0.44, 0.33 and 0.37 μg mL-1for SLB, RTD, IXP, DAP, SFM, and SFD, respectively.

Simultaneous Spectrophotometric Determination of Nifuroxime and Furazolidone in Pharmaceutical Preparations

1980 ◽  
Vol 63 (5) ◽  
pp. 992-995
Author(s):  
Laila Elsayed ◽  
Sayed M Hassan ◽  
Khadiga M Kelani ◽  
Hamed M El-Fatatry
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Determination ◽  
Pharmaceutical Preparations ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Separation Step ◽  
Preliminary Separation

Abstract Two spectrophotometric methods have been developed for the simultaneous determination of nifuroxime and furazolidone in their pharmaceutical preparations. No preliminary separation step is required in either method. The first, a modified Vierordt method, gives accurate and reproducible results for both drugs. Mean percent recoveries for nifuroxime and furazolidone were 99.50 ±1.59 and 100.20 ±1.16 (P = 0.05), respectively. This method also gives accurate and reproducible results for the determination of nifuroxime and furazolidone in their pharmaceutical preparations: Tricofuran vaginal suppositories and powder. The second method, which involves the use of the first-derivative curves, gives unreliable results; the reasons for these are discussed.

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