scholarly journals Analysis of differentially expressed genes during embryogenesis in ovary culture of cucumber (Cucumis sativus L.)

2019 ◽  
Keyword(s):  
Cucumis Sativus ◽  
Differentially Expressed Genes ◽  
Differentially Expressed ◽  
Ovary Culture ◽  
Cucumis Sativus L

scholarly journals Analysis of differentially expressed genes during embryogenesis in ovary culture of cucumber (Cucumis sativus L.)

2019 ◽  
Author(s):  
Ying Deng ◽  
Wenyuan Fu ◽  
Bing Tang ◽  
Lian Tao ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Cucumis Sativus ◽  
Differentially Expressed Genes ◽  
Shoot Formation ◽  
Differentially Expressed ◽  
Embryo Maturation ◽  
Ovary Culture ◽  
Cucumis Sativus L ◽  
Microtubule Binding ◽  
Formation Stage

Abstract Background: Ovary culture has been a useful way to generate double haploid (DH) plant in cucumber (Cucumis sativus L.). However, the rate of embryo induction is low, and the probability for the induced embryo to grow into normal embryo is low as well. This is largely due to unknown of the mechanism of embryogenesis in cucumber. In this study, the differentially expressed genes during embryogenesis, including the early stages of embryo formation, embryo maturation and shoot formation, was investigated with transcriptomic technique to set up basis for a more efficient ovary culture technology Results: Cytological observations led to suggestions that cell enlargement is the symbol that gametophytes had switched to the sporophyte development pathway during the early embryogenesis stage. In this stage, RNA-seq revealed 3468 up-regulated genes, including hormone signal transduction genes, hormone response genes and stress-induced genes. The reported embryogenesis-related genes BBM, HSP90 and AGL were also actively expressed during this stage. The total of 480 genes that function in protein complex binding, microtubule binding, tetrapyrrole binding, tubulin binding and other microtubule activities were continuously up-regulated during the embryo maturation stage, indicating that the cytoskeleton structure was continuously being built and maintained by the action of microtubule-binding proteins and enzyme modification during embryo development. In the shoot formation stage, 1383 genes were up-regulated, which were mainly enriched in phenylpropanoid biosynthesis, plant hormone signal transduction, phenylalanine metabolism, and starch and sucrose metabolism. The shoot formation stage might be regulated by 6 transcription factors that contained a B3 domain, 9 genes in the AP2/ERF family and 2 genes encoded WUS homologous domain proteins. Conclusions: Findings from this study offer a valuable framework for explaining the transcriptional regulatory mechanism underlying embryogenesis in cucumber ovary culture.

scholarly journals Identification of differentially expressed genes related to aphid resistance in cucumber (Cucumis sativus L.)

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Danna Liang ◽  
Min Liu ◽  
Qijing Hu ◽  
Min He ◽  
Xiaohua Qi ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Differentially Expressed Genes ◽  
Aphid Resistance ◽  
Differentially Expressed ◽  
Cucumis Sativus L

scholarly journals Shoots formation from gynosinesis Cucumis sativus l.

2020 ◽  
Vol 8 (1) ◽  
pp. 53-59
Author(s):  
Le Thi Kim Ngoc ◽  
Nguyen Tran Dong Phuong
Keyword(s):  
Cucumis Sativus ◽  
Callus Induction ◽  
Female Flower ◽  
Ovary Culture ◽  
Cold Pretreatment ◽  
Cucumis Sativus L ◽  
Leaves And Roots ◽  
Dark Conditions ◽  
Haploid Plants

Haploid plants achieve through androgenesis or gynogenesis. In gynogenesis method, the ovary or ovule are used as explants induct haploid plants. Female flower one day before flowering of Cucumis sativus L. are collected. Cold pretreatment of ovaries at 4°C up to 24 hours and culture under dark conditions. Significantly enhanced callus induction response is compared with cultures under 4-week cultured on CBM medium supplemented with various concentration of TDZ 0.01-0.04 mg/L. After 4 weeks, ovaries are transferred to medium with kinetin 0.05 – 0.20 mg/L. Then, ovaries were transferred to medium supplemented with BA: IAA 3:1. Finally, green ovaries were transferred to BA 1.5 mg/L and GA3 1.5 mg/L. The results showed that ovary induction has best affected on CBM with TDZ 0.03 mg/L with 11 callus/sample. Ovaries developed on kinetin 0.1 mg/L with 7.4 callus/sample. Ovaries become green and had leaves and roots formation on BA: IAA (3 mg/L: 1 mg/L). 11 plantlets were harvested from ovary culture after 12-week culture on CBM supplemented with BA 1.5 mg/L and GA3 1.5 mg/L.

Gynogenesis and doubled haploid production from unpollinated ovary culture of cucumber (Cucumis sativus L.)

2017 ◽  
Author(s):  
Atitaya Sorntip ◽  
Oythip Poolsawat ◽  
Chitpan Kativat ◽  
Piyada Alisha Tantasawat
Keyword(s):  
Cucumis Sativus ◽  
Doubled Haploid ◽  
Ovary Culture ◽  
Cucumis Sativus L ◽  
Haploid Production

scholarly journals Effect of Transgenesis on mRNA and miRNA Profiles in Cucumber Fruits Expressing Thaumatin II

Genes ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 334 ◽  
Author(s):  
Magdalena Ewa Pawełkowicz ◽  
Agnieszka Skarzyńska ◽  
Małgorzata Sroka ◽  
Maria Szwacka ◽  
Tomasz Pniewski ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Differentially Expressed ◽  
Transgenic Crop ◽  
Unintended Effects ◽  
Wild Type ◽  
Cucumis Sativus L ◽  
Breeding Programs ◽  
Minimal Impact ◽  
Gene Ontology Classification ◽  
Transgenic Lines

Transgenic plants are commonly used in breeding programs because of the various features that can be introduced. However, unintended effects caused by genetic transformation are still a topic of concern. This makes research on the nutritional safety of transgenic crop plants extremely interesting. Cucumber (Cucumis sativus L.) is a crop that is grown worldwide. The aim of this study was to identify and characterize differentially expressed genes and regulatory miRNAs in transgenic cucumber fruits that contain the thaumatin II gene, which encodes the sweet-tasting protein thaumatin II, by NGS sequencing. We compared the fruit transcriptomes and miRNomes of three transgenic cucumber lines with wild-type cucumber. In total, we found 47 differentially expressed genes between control and all three transgenic lines. We performed the bioinformatic functional analysis and gene ontology classification. We also identified 12 differentially regulated miRNAs, from which three can influence the two targets (assigned as DEGs) in one of the studied transgenic lines (line 224). We found that the transformation of cucumber with thaumatin II and expression of the transgene had minimal impact on gene expression and epigenetic regulation by miRNA, in the cucumber fruits.

Direct regeneration of haploid or doubled haploid plantlets in cucumber (Cucumis sativus L.) through ovary culture

2020 ◽  
Vol 142 (2) ◽  
pp. 253-268
Author(s):  
Ying Deng ◽  
Bing Tang ◽  
Xia Zhou ◽  
Wenyuan Fu ◽  
Lian Tao ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Doubled Haploid ◽  
Direct Regeneration ◽  
Ovary Culture ◽  
Cucumis Sativus L

scholarly journals Transcriptome analysis of ovary culture-induced embryogenesis in cucumber (Cucumis sativus L.)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12145
Author(s):  
Ying Deng ◽  
Wenyuan Fu ◽  
Bing Tang ◽  
Lian Tao ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Plant Regeneration ◽  
Cucumis Sativus ◽  
Transcriptome Sequencing ◽  
Shoot Formation ◽  
Early Embryo ◽  
Ovary Culture ◽  
Cucumis Sativus L ◽  
Microtubule Binding ◽  
Time Points

Background. Ovary culture is a useful technique used to generate double haploid (DH) cucumber (Cucumis sativus L.) plants. However, cucumber ovary culture have a low rate of embryo induction and plant regeneration. Moreover, the cucumber embryogenesis mechanism remains unclear. In this study, we explored the molecular basis of cucumber embryogenesis in order to establish a foundation for a more efficient ovary culture method. Using transcriptome sequencing, we also investigated the differential expression of genes during the embryogenesis process. Methods. Cytological and morphological observations have divided cucumber ovary culture into three stages: early embryo development (T0), embryo morphogenesis (T1, T2, T3 and T4), and shoot formation (T5). We selected six key time points for transcriptome sequencing and analysis: T0 (the ovules were cultured for 0 d), T1 (the ovules were cultured for 2 d), T2 (the embryos were cultured for 10 d), T3 (the embryos were cultured for 20 d), T4 (the embryos were cultured for 30 d), and T5 (the shoots after 60 d culture). Results. We used cytology and morphology to observe the characteristics of the cucumber’s developmental transformation during embryogenesis and plant regeneration. The differentially expressed genes(DEGs) at developmental transition points were analyzed using transcriptome sequencing. In the early embryogenesis stage, the cells expanded, which was the signal for gametophytes to switch to the sporophyte development pathway. RNA-seq revealed that when compared to the fresh unpollinated ovaries, there were 3,468 up-regulated genes in the embryos, including hormone signal transduction genes, hormone response genes, and stress-induced genes. The reported embryogenesis-related genes BBM, HSP90 and AGL were also actively expressed during this stage. In the embryo morphogenesis stage (from cell division to cotyledon-embryo formation), 480 genes that functioned in protein complex binding, microtubule binding, tetrapyrrole binding, tubulin binding and other microtubule activities were continuously up-regulated during the T1, T2, T3 and T4 time points. This indicated that the cytoskeleton structure was continuously being built and maintained by the action of microtubule-binding proteins and enzyme modification. In the shoot formation stage, 1,383 genes were up-regulated that were mainly enriched in phenylpropanoid biosynthesis, plant hormone signal transduction, phenylalanine metabolism, and starch and sucrose metabolism. These up-regualted genes included six transcription factors that contained a B3 domain, nine genes in the AP2/ERF family, and two genes encoding WUS homologous domain proteins. Conclusions. Evaluation of molecular gynogenesis events may contribute to a better understanding of the molecular mechanism of cucumber ovarian culture.

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