scholarly journals Identification of differentially expressed genes related to aphid resistance in cucumber (Cucumis sativus L.)

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Danna Liang ◽  
Min Liu ◽  
Qijing Hu ◽  
Min He ◽  
Xiaohua Qi ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Differentially Expressed Genes ◽  
Aphid Resistance ◽  
Differentially Expressed ◽  
Cucumis Sativus L

scholarly journals Analysis of differentially expressed genes during embryogenesis in ovary culture of cucumber (Cucumis sativus L.)

2019 ◽  
Author(s):  
Ying Deng ◽  
Wenyuan Fu ◽  
Bing Tang ◽  
Lian Tao ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Cucumis Sativus ◽  
Differentially Expressed Genes ◽  
Shoot Formation ◽  
Differentially Expressed ◽  
Embryo Maturation ◽  
Ovary Culture ◽  
Cucumis Sativus L ◽  
Microtubule Binding ◽  
Formation Stage

Abstract Background: Ovary culture has been a useful way to generate double haploid (DH) plant in cucumber (Cucumis sativus L.). However, the rate of embryo induction is low, and the probability for the induced embryo to grow into normal embryo is low as well. This is largely due to unknown of the mechanism of embryogenesis in cucumber. In this study, the differentially expressed genes during embryogenesis, including the early stages of embryo formation, embryo maturation and shoot formation, was investigated with transcriptomic technique to set up basis for a more efficient ovary culture technology Results: Cytological observations led to suggestions that cell enlargement is the symbol that gametophytes had switched to the sporophyte development pathway during the early embryogenesis stage. In this stage, RNA-seq revealed 3468 up-regulated genes, including hormone signal transduction genes, hormone response genes and stress-induced genes. The reported embryogenesis-related genes BBM, HSP90 and AGL were also actively expressed during this stage. The total of 480 genes that function in protein complex binding, microtubule binding, tetrapyrrole binding, tubulin binding and other microtubule activities were continuously up-regulated during the embryo maturation stage, indicating that the cytoskeleton structure was continuously being built and maintained by the action of microtubule-binding proteins and enzyme modification during embryo development. In the shoot formation stage, 1383 genes were up-regulated, which were mainly enriched in phenylpropanoid biosynthesis, plant hormone signal transduction, phenylalanine metabolism, and starch and sucrose metabolism. The shoot formation stage might be regulated by 6 transcription factors that contained a B3 domain, 9 genes in the AP2/ERF family and 2 genes encoded WUS homologous domain proteins. Conclusions: Findings from this study offer a valuable framework for explaining the transcriptional regulatory mechanism underlying embryogenesis in cucumber ovary culture.

scholarly journals Effect of Transgenesis on mRNA and miRNA Profiles in Cucumber Fruits Expressing Thaumatin II

Genes ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 334 ◽  
Author(s):  
Magdalena Ewa Pawełkowicz ◽  
Agnieszka Skarzyńska ◽  
Małgorzata Sroka ◽  
Maria Szwacka ◽  
Tomasz Pniewski ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Differentially Expressed ◽  
Transgenic Crop ◽  
Unintended Effects ◽  
Wild Type ◽  
Cucumis Sativus L ◽  
Breeding Programs ◽  
Minimal Impact ◽  
Gene Ontology Classification ◽  
Transgenic Lines

Transgenic plants are commonly used in breeding programs because of the various features that can be introduced. However, unintended effects caused by genetic transformation are still a topic of concern. This makes research on the nutritional safety of transgenic crop plants extremely interesting. Cucumber (Cucumis sativus L.) is a crop that is grown worldwide. The aim of this study was to identify and characterize differentially expressed genes and regulatory miRNAs in transgenic cucumber fruits that contain the thaumatin II gene, which encodes the sweet-tasting protein thaumatin II, by NGS sequencing. We compared the fruit transcriptomes and miRNomes of three transgenic cucumber lines with wild-type cucumber. In total, we found 47 differentially expressed genes between control and all three transgenic lines. We performed the bioinformatic functional analysis and gene ontology classification. We also identified 12 differentially regulated miRNAs, from which three can influence the two targets (assigned as DEGs) in one of the studied transgenic lines (line 224). We found that the transformation of cucumber with thaumatin II and expression of the transgene had minimal impact on gene expression and epigenetic regulation by miRNA, in the cucumber fruits.

scholarly journals Transcriptome analysis reveals ethylene-mediated defense responses to Fusarium oxysporum f. sp. cucumerinum infection in Cucumis sativus L.

2020 ◽  
Author(s):  
Jingping Dong ◽  
Yuean Wang ◽  
Qianqian Xian ◽  
Xuehao Chen ◽  
Jun Xu
Keyword(s):  
Fusarium Oxysporum ◽  
Cucumis Sativus ◽  
Defense Mechanisms ◽  
Severe Disease ◽  
Defense Responses ◽  
Ethylene Biosynthesis ◽  
Differentially Expressed ◽  
Pcr Analysis ◽  
Positive Role ◽  
Cucumis Sativus L

Abstract Background: Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown. To better understand of the defense mechanisms elicited in response to Foc inoculation, RNA sequencing-based transcriptomic profiling of responses of the Fusarium wilt-resistant cucumber line ‘Rijiecheng’ at 0, 24, 48, 96, and 192 hours after Foc inoculation was performed.Results: We identified 4116 genes that were differentially expressed between 0 hour and other time points after inoculation. All ethylene-related and pathogenesis-related genes from the differentially expressed genes were filtered out. Real-time PCR analysis showed that ethylene-related genes were induced in response to Foc infection. Importantly, after Foc infection and exogenous application of ethephon, a donor of ethylene, the ethylene-related genes were highly expressed. In response to exogenous ethephon treatment in conjunction with Foc inoculation, the infection resistance of cucumber seedlings was enhanced and endogenous ethylene biosynthesis increased dramatically. Conclusion: Collectively, ethylene signaling pathways play a positive role in regulating the defense response of cucumber to Foc infection. The results provide insight into the cucumber Fusarium wilt defense mechanisms and provide valuable information for breeding new cucumber cultivars with enhanced Fusarium wilt tolerance.

scholarly journals Transcriptome analysis reveals ethylene-mediated defense responses to Fusarium oxysporum f. sp. cucumerinum infection in Cucumis sativus L.

2020 ◽  
Author(s):  
Jingping Dong ◽  
Yuean Wang ◽  
Qianqian Xian ◽  
Xuehao Chen ◽  
Jun Xu
Keyword(s):  
Fusarium Oxysporum ◽  
Cucumis Sativus ◽  
Defense Mechanisms ◽  
Severe Disease ◽  
Defense Responses ◽  
Ethylene Biosynthesis ◽  
Differentially Expressed ◽  
Pcr Analysis ◽  
Positive Role ◽  
Cucumis Sativus L

Abstract Background: Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown. To better understand of the defense mechanisms elicited in response to Foc inoculation, RNA sequencing-based transcriptomic profiling of responses of the Fusarium wilt-resistant cucumber line ‘Rijiecheng’ at 0, 24, 48, 96, and 192 hours after Foc inoculation was performed.Results: We identified 4116 genes that were differentially expressed between 0 hour and other time points after inoculation. All ethylene-related and pathogenesis-related genes from the differentially expressed genes were filtered out. Real-time PCR analysis showed that ethylene-related genes were induced in response to Foc infection. Importantly, after Foc infection and exogenous application of ethephon, a donor of ethylene, the ethylene-related genes were highly expressed. In response to exogenous ethephon treatment in conjunction with Foc inoculation, the infection resistance of cucumber seedlings was enhanced and endogenous ethylene biosynthesis increased dramatically. Conclusion: Collectively, ethylene signaling pathways play a positive role in regulating the defense response of cucumber to Foc infection. The results provide insight into the cucumber Fusarium wilt defense mechanisms and provide valuable information for breeding new cucumber cultivars with enhanced Fusarium wilt tolerance.

scholarly journals Transcriptome analysis reveals ethylene-mediated defense responses to Fusarium oxysporum f. sp. cucumerinum infection in Cucumis sativus L.

2020 ◽  
Author(s):  
Jingping Dong ◽  
Yuean Wang ◽  
Qianqian Xian ◽  
Xuehao Chen ◽  
Jun Xu
Keyword(s):  
Fusarium Oxysporum ◽  
Cucumis Sativus ◽  
Defense Mechanisms ◽  
Severe Disease ◽  
Defense Responses ◽  
Ethylene Biosynthesis ◽  
Differentially Expressed ◽  
Pcr Analysis ◽  
Positive Role ◽  
Cucumis Sativus L

Abstract Background: Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown. To better understand of the defense mechanisms elicited in response to Foc inoculation, RNA sequencing-based transcriptomic profiling of responses of the Fusarium wilt-resistant cucumber line ‘Rijiecheng’ at 0, 24, 48, 96, and 192 hours after Foc inoculation was performed.Results: We identified 4116 genes that were differentially expressed between 0 hour and other time points after inoculation. All ethylene-related and pathogenesis-related genes from the differentially expressed genes were filtered out. Real-time PCR analysis showed that ethylene-related genes were induced in response to Foc infection. Importantly, after Foc infection and exogenous application of ethephon, a donor of ethylene, the ethylene-related genes were highly expressed. In response to exogenous ethephon treatment in conjunction with Foc inoculation, the infection resistance of cucumber seedlings was enhanced and endogenous ethylene biosynthesis increased dramatically. Conclusion: Collectively, ethylene signaling pathways play a positive role in regulating the defense response of cucumber to Foc infection. The results provide insight into the cucumber Fusarium wilt defense mechanisms and provide valuable information for breeding new cucumber cultivars with enhanced Fusarium wilt tolerance.

Sign in / Sign up

Export Citation Format

Share Document