Nasopharyngeal carriage, spa types and antibiotic susceptibility profiles of Staphylococcus aureus from healthy children less than 5 years in Eastern Uganda

Abstract Background: Staphylococcus aureus carriage is a known risk factor for staphylococcal disease. However, the carriage rates vary by country, demographic group and profession. This study aimed to determine the S. aureus carriage rate in children in Eastern Uganda, and identify S. aureus lineages that cause infection in Uganda. Methods: Nasopharyngeal samples from 742 healthy children less than 5 years residing in the Iganga/Mayuge Health and Demographic Surveillance Site in Eastern Uganda were processed for isolation of S. aureus. Antibiotic susceptibility testing based on minimum inhibitory concentrations (MICs) was determined by the BD PhoenixTM system. Genotyping was performed by spa and SCCmec typing. Results: The processed samples yielded 144 S. aureus isolates (one per child) therefore, the S. aureus carriage rate in children was 19.4% (144/742). Thirty one percent (45/144) of the isolates were methicillin resistant (MRSA) yielding a carriage rate of 6.1% (45/742). All isolates were susceptible to rifampicin, vancomycin and linezolid. Moreover, all MRSA were susceptible to vancomycin, linezolid and clindamycin. Compared to methicillin susceptible S. aureus (MSSA) isolates (68.8%, 99/144), MRSA isolates were more resistant to non-beta-lactam antimicrobials –trimethoprim/sulfamethoxazole 73.3% (33/45) vs. 27.3% (27/99) [p<0.0001]; erythromycin 75.6% (34/45) vs. 24.2% (24/99) [p<0.0001]; chloramphenicol 60% (27/45) vs. 19.2% (19/99) [p<0.0001]; gentamicin 55.6% (25/45) vs. 25.3% (25/99) [p=0.0004]; and ciprofloxacin 35.6% (16/45) vs. 2% (2/99) [p<0.0001]. Furthermore, 42 MRSA (93.3%) were multidrug resistant (MDR) and one exhibited high-level resistance to mupirocin. Overall, 61 MSSA (61.6%) were MDR, including three mupirocin and clindamycin resistant isolates. Seven spa types were detected among MRSA, of which t037 and t064 were predominant and associated with SCCmec types I and IV, respectively. Fourteen spa types were detected in MSSA which consisted mainly of t645 and t4353. Conclusions: S. aureus carriage rate in healthy children in Eastern Uganda is high and comparable to rates for hospitalized patients in Kampala. The detection of mupirocin resistance is worrying as it could rapidly increase if mupirocin is administered in a low-income setting. S. aureus strains of spa types t064, t037 (MRSA) and t645, t4353 (MSSA) are prevalent and could be responsible for majority of staphylococcal infections in Uganda.

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Nasopharyngeal carriage, spa types and antibiotic susceptibility profiles of Staphylococcus aureus from healthy children less than 5 years in Eastern Uganda

BMC Infectious Diseases ◽

10.1186/s12879-019-4652-5 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

David Patrick Kateete ◽

Benon B. Asiimwe ◽

Raymond Mayanja ◽

Brian Mujuni ◽

Freddie Bwanga ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Low Income ◽

Antibiotic Susceptibility ◽

Multidrug Resistant ◽

Healthy Children ◽

Beta Lactam ◽

Carriage Rate ◽

High Level ◽

Susceptibility Profiles ◽

Eastern Uganda

Abstract Background Staphylococcus aureus carriage is a known risk factor for staphylococcal disease. However, the carriage rates vary by country, demographic group and profession. This study aimed to determine the S. aureus carriage rate in children in Eastern Uganda, and identify S. aureus lineages that cause infection in Uganda. Methods Nasopharyngeal samples from 742 healthy children less than 5 years residing in the Iganga/Mayuge Health and Demographic Surveillance Site in Eastern Uganda were processed for isolation of S. aureus. Antibiotic susceptibility testing based on minimum inhibitory concentrations (MICs) was determined by the BD Phoenix™ system. Genotyping was performed by spa and SCCmec typing. Results The processed samples yielded 144 S. aureus isolates (one per child) therefore, the S. aureus carriage rate in children was 19.4% (144/742). Thirty one percent (45/144) of the isolates were methicillin resistant (MRSA) yielding a carriage rate of 6.1% (45/742). All isolates were susceptible to rifampicin, vancomycin and linezolid. Moreover, all MRSA were susceptible to vancomycin, linezolid and clindamycin. Compared to methicillin susceptible S. aureus (MSSA) isolates (68.8%, 99/144), MRSA isolates were more resistant to non-beta-lactam antimicrobials –trimethoprim/sulfamethoxazole 73.3% (33/45) vs. 27.3% (27/99) [p < 0.0001]; erythromycin 75.6% (34/45) vs. 24.2% (24/99) [p < 0.0001]; chloramphenicol 60% (27/45) vs. 19.2% (19/99) [p < 0.0001]; gentamicin 55.6% (25/45) vs. 25.3% (25/99) [p = 0.0004]; and ciprofloxacin 35.6% (16/45) vs. 2% (2/99) [p < 0.0001]. Furthermore, 42 MRSA (93.3%) were multidrug resistant (MDR) and one exhibited high-level resistance to mupirocin. Overall, 61 MSSA (61.6%) were MDR, including three mupirocin and clindamycin resistant isolates. Seven spa types were detected among MRSA, of which t037 and t064 were predominant and associated with SCCmec types I and IV, respectively. Fourteen spa types were detected in MSSA which consisted mainly of t645 and t4353. Conclusions S. aureus carriage rate in healthy children in Eastern Uganda is high and comparable to rates for hospitalized patients in Kampala. The detection of mupirocin resistance is worrying as it could rapidly increase if mupirocin is administered in a low-income setting. S. aureus strains of spa types t064, t037 (MRSA) and t645, t4353 (MSSA) are prevalent and could be responsible for majority of staphylococcal infections in Uganda.

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Nasopharyngeal carriage, spa types and antibiotic susceptibility profiles of Staphylococcus aureus from healthy children less than 5 years in Eastern Uganda

10.21203/rs.2.10945/v4 ◽

2019 ◽

Author(s):

David Kateete ◽

Benon B Asiimwe ◽

Raymond Mayanja ◽

Brian Mujuni ◽

Freddie Bwanga ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Low Income ◽

Antibiotic Susceptibility ◽

Multidrug Resistant ◽

Healthy Children ◽

Beta Lactam ◽

Spa Typing ◽

Carriage Rate ◽

Eastern Uganda ◽

Spa Types

Abstract Background: Staphylococcus aureus carriage is a known risk factor for staphylococcal disease. However, the carriage rates vary by country, demographic group and profession. This study aimed to determine the S. aureus carriage rate in children in Eastern Uganda, and identify S. aureus lineages that cause infection in Uganda. Methods: Nasopharyngeal samples from 742 healthy children less than 5 years residing in the Iganga/Mayuge Health & Demographic Surveillance Site in Eastern Uganda were processed for isolation of S. aureus. Antibiotic susceptibility testing based on minimum inhibitory concentrations (MICs) was determined by the BD PhoenixTM system. Genotyping was performed by spa typing. Results: The processed samples yielded 144 S. aureus isolates (one per child) therefore, the S. aureus carriage rate in children was 19.4% (144/742). Thirty one percent (45/144) of the isolates were methicillin resistant (MRSA) yielding a carriage rate of 6.1% (45/742). All isolates were susceptible to rifampicin, vancomycin and linezolid. Moreover, all MRSA were susceptible to vancomycin, linezolid and clindamycin. Compared to methicillin susceptible S. aureus (MSSA) isolates (68.8%, 99/144), MRSA isolates were more resistant to non-beta-lactam antimicrobials –trimethoprim/sulfamethoxazole 73.3% (33/45) vs. 27.3% (27/99) [P<0.0001]; erythromycin 75.6% (34/45) vs. 24.2% (24/99) [P<0.0001]; chloramphenicol 60% (27/45) vs. 19.2% (19/99) [P<0.0001]; gentamicin 55.6% (25/45) vs. 25.3% (25/99) [P=0.0004]; and ciprofloxacin 35.6% (16/45) vs. 2% (2/99) [P<0.0001]. Furthermore, 42 MRSA (93.3%) were multidrug resistant (MDR) and one exhibited high-level resistance to mupirocin. Overall, 61 MSSA (61.6%) were MDR, including three mupirocin and clindamycin resistant isolates. Seven spa types were detected in MRSA, of which t037 & t064 were predominant and associated with SCCmec types I & IV, respectively. Fourteen spa types were detected in MSSA, of which t645 & t4353 were predominant. Conclusions: S. aureus carriage rate in healthy children in Eastern Uganda is high and comparable to rates for hospitalized patients in Kampala. The detection of mupirocin resistance is worrying as it could rapidly increase if mupirocin is administered in a low-income setting. S. aureus strains of spa types t064, t037 (MRSA) and t645, t4353 (MSSA) are prevalent and could be responsible for majority of staphylococcal infections in Uganda.

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Nasopharyngeal carriage, spa types and drug susceptibility profiles of Staphylococcus aureus from healthy children under 5 years in eastern Uganda

10.21203/rs.2.10945/v1 ◽

2019 ◽

Author(s):

David Kateete ◽

Benon B Asiimwe ◽

Raymond Mayanja ◽

Brian Mujuni ◽

Freddie Bwanga ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Low Income ◽

Pulse Field ◽

Healthy Children ◽

Beta Lactam ◽

Spa Typing ◽

Carriage Rate ◽

Eastern Uganda ◽

Spa Types ◽

Children Under 5

Abstract Background Staphylococcus aureus carriage is a known risk factor for staphylococcal disease. However, the carriage rates vary by country, demographic group, etc. This study aimed to determine the S. aureus carriage rate in Ugandan children, and track Staphylococcus strains that can cause infection in Uganda. Methods Nasopharyngeal samples (one per child) from 742 healthy children under 5 years living in Iganga/Mayuge Health & Demographic Surveillance Site in eastern Uganda were processed for isolation of S. aureus. Genotyping was performed by spa typing and pulse field gel electrophoresis. Results The processed samples yielded 144 S. aureus isolates (one per sample/child) therefore, the S. aureus carriage rate in the children was 19.4% (144/742). Further, 45 (31.3%, 45/144) of the isolates were methicillin resistant (MRSA) yielding a carriage rate of 6.1% (45/742). All MRSA isolates were susceptible to vancomycin, linezolid and clindamycin however, compared to methicillin susceptible S. aureus (MSSA) isolates (68.8%, 99/144), MRSA were more resistant to non-beta-lactam antimicrobials –tetracycline (91.1%, 41/45), trimethoprim/sulfamethoxazole (73.3%, 33/45), erythromycin (75.6%, 34/99), chloramphenicol (60%, 19/99), gentamicin (55.6%, 25/45) and ciprofloxacin (35.6%, 16/45). Furthermore, an MRSA isolate was mupirocin resistant and 42 (93.3%, 42/45) were multidrug resistant (MDR); three (3%, 3/99) MSSA isolates were mupirocin and clindamycin resistant while 61 (61.6%, 61/99) were MDR. All MSSA/MRSA isolates were susceptible to rifampicin, vancomycin and linezolid but only two were pan-susceptible to the tested antibiotics. Seven spa types were detected in MRSA, of which t064 & t037 were predominant and associated with SCCmec types I & IV, respectively. Fourteen spa types were detected in MSSA, of which t645 & t4353 were predominant. Conclusions S. aureus (MSSA/MRSA) carriage rate in children in rural eastern Uganda is high and comparable to rates for hospitalized patients in Kampala city. Detection of mupirocin resistance is worrying as it could rapidly increase in a low-income setting should mupirocin be used for staphylococcal decolonization. Further, S. aureus strains of spa types t064, t037, t645 & t4353 are prevalent and could be responsible for majority of staphylococcal infections in Uganda.

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Nasopharyngeal carriage, spa types and drug susceptibility profiles of Staphylococcus aureus from healthy children under 5 years in eastern Uganda

10.21203/rs.2.10945/v2 ◽

2019 ◽

Author(s):

David Kateete ◽

Benon B Asiimwe ◽

Raymond Mayanja ◽

Brian Mujuni ◽

Freddie Bwanga ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Low Income ◽

Drug Susceptibility ◽

Healthy Children ◽

Beta Lactam ◽

Spa Typing ◽

Carriage Rate ◽

Eastern Uganda ◽

Spa Types ◽

Children Under 5

Abstract Background: Staphylococcus aureus carriage is a known risk factor for staphylococcal disease. However, the carriage rates vary by country, demographic group and profession. This study aimed to determine the S. aureus carriage rate in children in Eastern Uganda, and track Staphylococcus strains that can cause infection in Uganda. Methods: Nasopharyngeal samples from 742 healthy children under 5 years residing in the Iganga/Mayuge Health & Demographic Surveillance Site in Eastern Uganda were processed for isolation of S. aureus. Antibiotic resistance based on minimum inhibitory concentrations (MICs) was determined by the BD Phoenix™ automated identification & susceptibility testing system. Genotyping was performed by spa typing. Results: The processed samples yielded 144 S. aureus isolates (one per child) therefore, the S. aureus carriage rate in children was 19.4% (144/742). Thirty one percent (45/144) of the isolates were methicillin resistant (MRSA) yielding a carriage rate of 6.1% (45/742). All MRSA isolates were susceptible to vancomycin, linezolid and clindamycin however, compared to methicillin susceptible S. aureus (MSSA) (68.8%, 99/144), MRSA isolates were more resistant to non-beta-lactam antimicrobials –tetracycline (91.1%, 41/45), trimethoprim/sulfamethoxazole (73.3%, 33/45), erythromycin (75.6%, 34/45), chloramphenicol (60%, 27/45), gentamicin (55.6%, 25/45) and ciprofloxacin (35.6%, 16/45). Furthermore, one MRSA isolate was mupirocin resistant and 42 (93.3%, 42/45) were multidrug resistant (MDR); three (3%, 3/99) MSSA isolates were mupirocin and clindamycin resistant, while 61 (61.6%, 61/99) were MDR. All MSSA/MRSA isolates were susceptible to rifampicin, vancomycin and linezolid but only three MSSA were pan-susceptible to antibiotics. Seven spa types were detected in MRSA, of which t037 & t064 were predominant and associated with SCCmec types I & IV, respectively. Fourteen spa types were detected in MSSA, of which t645 & t4353 were predominant. Conclusions: S. aureus (MSSA/MRSA) carriage rate in children in Eastern Uganda is high and comparable to rates for hospitalized patients in Kampala city. The detection of mupirocin resistance is worrying as it could rapidly increase if mupirocin is administered in a low-income setting. S. aureus strains of spa types t064, t037 (MRSA) and t645, t4353 (MSSA) are prevalent and could be responsible for majority of staphylococcal infections in Uganda.

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Nasopharyngeal carriage, spa types and drug susceptibility profiles of Staphylococcus aureus from healthy children under 5 years in eastern Uganda

10.21203/rs.2.10945/v3 ◽

2019 ◽

Author(s):

David Kateete ◽

Benon B Asiimwe ◽

Raymond Mayanja ◽

Brian Mujuni ◽

Freddie Bwanga ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Low Income ◽

Drug Susceptibility ◽

Healthy Children ◽

Beta Lactam ◽

Spa Typing ◽

Carriage Rate ◽

Eastern Uganda ◽

Spa Types ◽

Children Under 5

Abstract Background: Staphylococcus aureus carriage is a known risk factor for staphylococcal disease. However, the carriage rates vary by country, demographic group and profession. This study aimed to determine the S. aureus carriage rate in children in Eastern Uganda, and track Staphylococcus strains that can cause infection in Uganda. Methods: Nasopharyngeal samples from 742 healthy children under 5 years residing in the Iganga/Mayuge Health & Demographic Surveillance Site in Eastern Uganda were processed for isolation of S. aureus. Antibiotic resistance based on minimum inhibitory concentrations (MICs) was determined by the BD Phoenix™ automated identification & susceptibility testing system. Genotyping was performed by spa typing. Results: The processed samples yielded 144 S. aureus isolates (one per child) therefore, the S. aureus carriage rate in children was 19.4% (144/742). Thirty one percent (45/144) of the isolates were methicillin resistant (MRSA) yielding a carriage rate of 6.1% (45/742). All MRSA isolates were susceptible to vancomycin, linezolid and clindamycin however, compared to methicillin susceptible S. aureus (MSSA) (68.8%, 99/144), MRSA isolates were more resistant to non-beta-lactam antimicrobials –tetracycline (91.1%, 41/45), trimethoprim/sulfamethoxazole (73.3%, 33/45), erythromycin (75.6%, 34/45), chloramphenicol (60%, 27/45), gentamicin (55.6%, 25/45) and ciprofloxacin (35.6%, 16/45). Furthermore, one MRSA isolate was mupirocin resistant and 42 (93.3%, 42/45) were multidrug resistant (MDR); three (3%, 3/99) MSSA isolates were mupirocin and clindamycin resistant, while 61 (61.6%, 61/99) were MDR. All MSSA/MRSA isolates were susceptible to rifampicin, vancomycin and linezolid but only three MSSA were pan-susceptible to antibiotics. Seven spa types were detected in MRSA, of which t037 & t064 were predominant and associated with SCCmec types I & IV, respectively. Fourteen spa types were detected in MSSA, of which t645 & t4353 were predominant. Conclusions: S. aureus (MSSA/MRSA) carriage rate in children in Eastern Uganda is high and comparable to rates for hospitalized patients in Kampala city. The detection of mupirocin resistance is worrying as it could rapidly increase if mupirocin is administered in a low-income setting. S. aureus strains of spa types t064, t037 (MRSA) and t645, t4353 (MSSA) are prevalent and could be responsible for majority of staphylococcal infections in Uganda.

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Antibiotic Susceptibility of Staphylococcus aureus Isolated from Retailed Raw Beef at Choba Market, Rivers State

International Journal of Pathogen Research ◽

10.9734/ijpr/2021/v6i430168 ◽

2021 ◽

pp. 25-30

Author(s):

Onoriode C. Eruteya ◽

Patience O. Osariemen

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Susceptibility ◽

Multidrug Resistant ◽

Antibiotic Resistant ◽

Mannitol Salt Agar ◽

Randomized Design ◽

Mueller Hinton ◽

Mueller Hinton Agar ◽

High Level ◽

Rivers State

Aims: The aim of the study was to determine the presence of antibiotic resistant Staphylococcus aureus in retail raw beef in Choba market, Nigeria. Study design: The study is based on a completely randomized design with two replicates and the mean being calculated. Place and Duration of Study: Major’s Laboratory, Department of Microbiology, University of Port Harcourt within three months. Methodology: The presence, characterization and antibiotic susceptibility of S. aureus from 30 retail raw beef samples was done using standard microbiological method involving the use of mannitol salt agar (MSA) and Mueller Hinton agar (MHA). Results: All the samples were positive for Staphylococcus species of which 18 (60.00%) were positive for S. aureus. The S. aureus strains were least resistant to gentamycin (22.22%) and cotrimoxazole (38.89%) with varying resistance against erythromycin (83.33%), tetracycline (88.88) and chloramphenicol (61.11%). All the S. aureus isolates in this study were 100% resistant to cloxacillin, amoxicillin and augmentin or amoxicillin-clavulanic acid combination. These results also showed the potential dissemination of multidrug-resistant S. aureus strains in the raw beef samples examined. Conclusion: The results of this study suggested high-level contamination of meat with multi-drug resistant S. aureus and this highlights the public health consequences associated with consuming such unhygienic products if poorly prepared.

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Spectral and Antibiotic Susceptibility of Pathogens Isolated from Saudi Patients with Diabetic Foot Infections

Microbiology Research ◽

10.3390/microbiolres12010002 ◽

2021 ◽

Vol 12 (1) ◽

pp. 16-20

Author(s):

Samiah Hamad S Al-Mijalli

Keyword(s):

Staphylococcus Aureus ◽

Diabetic Foot ◽

Antibiotic Susceptibility ◽

Empirical Therapy ◽

Multidrug Resistant ◽

Foot Ulcers ◽

Sensitivity Testing ◽

Cross Sectional ◽

Diabetic Foot Infections ◽

Microbiological Methods

Diabetic foot infections (DFIs) are a significant health issue and a common complication among patients with diabetes. To develop antibiotic therapy for these high-risk patients, the current study evaluates the scope of DFIs and identifies the causing microbes. It also measures spectrum and antibiotic susceptibility of the pathogens isolated from adults with DFIs in Saudi Arabia. To achieve the study objectives, a cross-sectional study was implemented and the baseline characteristics for 44 patients with DFIs were defined. Optimal aerobic and anaerobic microbiological techniques were utilized to culture specimens isolated from infected foot ulcers. The standard microbiological methods were employed to identify the bacterial isolates and antibiotic susceptibility testing was conducted following the procedures of the Clinical and Laboratory Standards Institute (CLSI). Results showed that 12 microorganisms were isolated from the participants’ diabetic foot ulcers. Staphylococcus Aureus was ranked first because it appeared in 29 (65.9%) cases. Streptococcus Agalactiae was ranked second and multi-microbial infections were also found. Most of the organisms were susceptible to Vancomycin, Ciprofloxacin, and Cefalexin, but they were resistant to Methicillin, Gentamicin, and Ampicillin antibiotics. Staphylococcus Aureus was most sensitive to Ciprofloxacin, while it was resistant to Methicillin. About 10% of the isolates were multidrug-resistant. The study concludes that while Vancomycin should be used empirically for Gram-positive isolates, Ciprofloxacin can be taken into consideration for most of the Gram-negatives aerobes. Based on including various microorganisms and the advent of multidrug-resistant strains, proper culture and sensitivity testing are necessary prior to the empirical therapy.

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