scholarly journals Evidence of pathogenic zoonotic tick-borne Rickettsia and Borrelia spp. in some communal farms in the Eastern Cape Province, South Africa

2020 ◽  
Author(s):  
OLUSESAN ADELABU ◽  
Benson Chuks Iweriebor ◽  
Anthony Ifeanyi Okoh ◽  
Larry Chikwelu Obi
Keyword(s):  
South Africa ◽  
Human Health ◽  
Molecular Identification ◽  
Domestic Animals ◽  
12S Rrna ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
Domesticated Animals ◽  
Identification Techniques ◽  
Reference Strains

Abstract The abundance of tick populations in South Africa represents a probable risk for both animal and human health. Rickettsia spp. and Borrelia spp. are well-known significant agents of emerging human tick-borne infectious diseases throughout the world. Nevertheless, the epidemiology of their infections has been underreported in South Africa, therefore, the aim of this study was to profile for zoonotic Rickettsia and Borrelia species from ticks infesting domesticated animals in the Eastern Cape, South Africa. Morphological and molecular identification techniques were conducted on 1,200 tick samples collected from domestic animals before screening for the target bacterial pathogens. The molecular identification of the tick samples was based on the amplification of the 12S rRNA mitochondrial DNA while those of Rickettsia and Borrelia species were carried out by amplifying fragments of gltA, ompA and ompB genes for Rickettsia and flaB gene for Borrelia spp. Thereafter, the positive ticks, Borrelia and Rickettsia ompB amplicons were sequenced and further analysed. Eight species of ticks belonging to three genera; Rhipicephalus, Amblyomma, and Haemaphysalis were identified. A total of 320 (27%) samples were confirmed positive for Rickettsia out of which 74 (23%) were positive for both ompA and ompB genes. Phylogenetic analysis of ompB revealed a high homology to rickettsial reference strains from GenBank, while there was no positive result for Borrelia. The generated sequences showed 99.1 to 100% homology with R. africae-KX227790 (100 %), R. parkeri - KY113111 (99.8 %), R. peacock (99.3 %) and R. slovaca - KJ675445, JX683122 (99.1 %) representative sequences in GenBank. The findings from this study revealed that ticks collected from domesticated animals were parasitized by Rickettsia species with possible zoonotic potential, which is detrimental to human health if bitten by infected ticks.

scholarly journals Evidence of pathogenic zoonotic tick-borne Rickettsia and Borrelia spp.  in some communal farms in the Eastern Cape Province, South Africa

2019 ◽  
Author(s):  
Olusesan Adeyemi Adelabu ◽  
Benson Chuks Iweriebor ◽  
Anthony Ifeanyi Okoh ◽  
Larry Chikwelu Obi
Keyword(s):  
South Africa ◽  
Human Health ◽  
Molecular Identification ◽  
Domestic Animals ◽  
12S Rrna ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
Domesticated Animals ◽  
Identification Techniques ◽  
Reference Strains

Abstract BackgroundThe abundance of tick populations in South Africa represents a probable risk for both animal and human health. Rickettsia and Borrelia are well-known significant agents of emerging human tick-borne infectious diseases throughout the world. Nevertheless, the epidemiology of their infections has been underreported in South Africa, Therefore, the aim of this study was to profile for zoonotic Rickettsia and Borrelia species from ticks infesting domesticated animals in the Eastern Cape, South Africa. Morphological and molecular identification techniques were conducted on 1,200 tick samples collected from domestic animals before screening for the target bacterial pathogens. ResultsThe molecular identification of the tick samples was based on the amplification of the 12S rRNA mitochondrial DNA while detection of Rickettsia and Borrelia species were carried out by amplifying fragments of gltA, ompA and ompB genes for Rickettsia and flaB gene for Borrelia spp. Thereafter, the positive ticks, Borrelia and Rickettsia ompB amplicons were sequenced and further analysed. Eight species of ticks belonging to three genera; Rhipicephalus, Amblyomma, and Haemaphysalis were identified. A total of 320 (27%) samples were confirmed positive for Rickettsia out of which 74 (23%) were positive for both ompA and ompB genes. Phylogenetic analysis of ompB revealed a high homology to rickettsial reference strains from GenBank, while there was no positive result for Borrelia. The generated sequences showed 99.1 to 100% homology with R. africae-KX227790 (100 %), R. parkeri - KY113111 (99.8 %), R .peacock (99.3 %) and R. slovaca - KJ675445, JX683122 (99.1 %) representative sequences in GenBank. ConclusionThe findings from this study revealed that ticks collected from domesticated animals were parasitized by Rickettsia species with possible zoonotic potential, which is detrimental to human health if bitten by infected ticks.

scholarly journals Evidence of pathogenic zoonotic tick-borne Rickettsia and Borrelia spp. in some communal farms in the Eastern Cape Province, South Africa

2019 ◽  
Author(s):  
OLUSESAN ADELABU ◽  
Benson Chuks Iweriebor ◽  
Larry Chikwelu Obi ◽  
Anthony Ifeanyi Okoh
Keyword(s):  
South Africa ◽  
Human Health ◽  
Molecular Identification ◽  
12S Rrna ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
Rickettsia Species ◽  
Domesticated Animals ◽  
Identification Techniques ◽  
Reference Strains

Abstract The abundance of tick populations in South Africa represents a probable risk for both animal and human health. Rickettsia and Borrelia are well-known significant agents of emerging human tick-borne infectious diseases throughout the world. Nevertheless, the epidemiology of their infections has been underreported in South Africa, Therefore, the aim of this study was to profile for zoonotic Rickettsia and Borrelia species from ticks infesting domesticated animals in the Eastern Cape, South Africa. Morphological and molecular identification techniques were conducted on 1,200 tick samples collected from domestic animals before screening for the target bacterial pathogens. The molecular identification of the tick samples was based on the amplification of the 12S rRNA mitochondrial DNA while those of Rickettsia and Borrelia species were carried out by amplifying fragments of glt A, omp A and omp B genes for Rickettsia and fla B gene for Borrelia spp. Thereafter, the positive ticks, Borrelia and Rickettsia omp B amplicons were sequenced and further analysed. Eight species of ticks belonging to three genera; Rhipicephalus, Amblyomma, and Haemaphysalis were identified. A total of 320 (27%) samples were confirmed positive for Rickettsia out of which 74 (23%) were positive for both omp A and omp B genes. Phylogenetic analysis of omp B revealed a high homology to rickettsial reference strains from GenBank, while there was no positive result for Borrelia. The generated sequences showed 99.1 to 100% homology with R. africae -KX227790 (100%), R. parkeri - KY113111 (99.8%), R .peacock (99.3%) and R. slovaca - KJ675445, JX683122 (99.1%) representative sequences in GenBank. The findings from this study revealed that ticks collected from domesticated animals were parasitized by Rickettsia species with possible zoonotic potential, which is detrimental to human health if bitten by infected ticks.

The recovery and molecular identification of HAdV-D17 in raw sewage and mussel samples collected in the Eastern Cape province of South Africa

2018 ◽  
Vol 33 (1) ◽  
pp. 4-7 ◽  
Author(s):  
Hillary J. Vos ◽  
Caroline M. Knox
Keyword(s):  
South Africa ◽  
Molecular Identification ◽  
Pcr Amplification ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
Hexon Gene ◽  
Specific Primers ◽  
Blast Analysis ◽  
Lack Of Information ◽  
Transmission Electron

Human adenoviruses (HAdV) are a common cause of clinical infections in South Africa. However, there is a lack of information regarding the prevalence and molecular identification of this virus in the environment. The objective of this study was to investigate the recovery and molecular identification of HAdV in sewage and mussel samples. All samples were subjected to transmission electron microscopy, viral DNA extraction and nested PCR amplification using adenovirus-specific primers targeting a conserved region of the hexon gene. Amplicons were cloned and sequenced and BLAST analysis revealed a closest matched sequence (98% identity) belonging to HAdV-D17.

Influence of Knowledge Management Practices on Public Service Delivery by Municipalities

Mousaion ◽  
2019 ◽  
Vol 37 (3) ◽  
Author(s):  
Charleen Musonza ◽  
Ndakasharwa Muchaonyerwa
Keyword(s):  
South Africa ◽  
Knowledge Management ◽  
Service Delivery ◽  
Public Service ◽  
Qualitative Data ◽  
Management Practices ◽  
Integrated System ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
Public Service Delivery

This study examines the influence of knowledge management (KM) practices on public service delivery by municipalities in the Eastern Cape province of South Africa. The study sought to determine the factors that have triggered the implementation of KM practices; the effectiveness of KM practices towards public service delivery; and the extent to which KM practices have influenced public service delivery by municipalities in the Eastern Cape province of South Africa. Both quantitative and qualitative methods were employed in this study. Quantitative data were collected through a survey questionnaire administered to a sample of 202 employees at the Raymond Mhlaba Municipality in the Eastern Cape. Qualitative data were collected through observations and interviews of 2 senior managers. The data collected gave a response rate of 72 per cent. The quantitative and qualitative data were analysed descriptively and presented verbatim respectively. The results indicated that the internal and external factors included in this study have contributed to the implementation of KM practices in the municipality. Furthermore, the effective use of KM practices has increased the organisational KM initiative, as well as the provision of services such as electricity, education, transport, and social services by the municipality. The study recommends the establishment of KM awareness and the establishment of an integrated system that will assist in effective knowledge sharing, retention and acquisition across municipalities in the Eastern Cape.

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