scholarly journals Ascending noradrenergic excitation from the locus coeruleus to the anterior cingulate cortex

2020 ◽  
Author(s):  
Kohei Koga ◽  
Akihiro Yamada ◽  
Qian Song ◽  
Xu-Hui Li ◽  
Qi-Yu Chen ◽  
...  
Keyword(s):  
Locus Coeruleus ◽  
Anterior Cingulate Cortex ◽  
Behavioral Sensitization ◽  
Pyramidal Cells ◽  
Cingulate Cortex ◽  
Anterior Cingulate ◽  
Subcortical Structures ◽  
Electron Microscopic ◽  
Excitatory Transmission

Abstract Anterior cingulate cortex (ACC) plays important roles in sensory perception including pain and itch. Neurons in the ACC receive various neuromodulatory inputs from subcortical structures, including locus coeruleus noradrenaline (LC-NA) neurons. Few studies have been reported about synaptic and behavioral functions of LC-NA projections to the ACC. Using viral-genetic method (AAV-DIO-eYFP) on DBH-cre mice, we found that LC-NA formed synaptic connections to ACC pyramidal cells but not interneurons. This is further supported by the electron microscopic study showing NAergic fibers contact the presynaptic inputs and post-synaptic areas of the pyramidal cells. NA application produced both pre- and post-synaptic potentiation effects in ACC excitatory transmission in vivo and in vitro . Activation of LC-NA projection to the ACC by optogenetic method produced enhancement of excitatory transmission in vitro and induced scratching and behavioral sensitization for mechanical stimulation. Our results demonstrate that LC-NA projections enhance or facilitate brain responses to pain and itch by potentiating glutamatergic synaptic transmissions in the ACC.

scholarly journals Ascending noradrenergic excitation from the locus coeruleus to the anterior cingulate cortex

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kohei Koga ◽  
Akihiro Yamada ◽  
Qian Song ◽  
Xu-Hui Li ◽  
Qi-Yu Chen ◽  
...  
Keyword(s):  
Locus Coeruleus ◽  
Anterior Cingulate Cortex ◽  
Behavioral Sensitization ◽  
Pyramidal Cells ◽  
Cingulate Cortex ◽  
Anterior Cingulate ◽  
Subcortical Structures ◽  
Electron Microscopic ◽  
Excitatory Transmission

AbstractAnterior cingulate cortex (ACC) plays important roles in sensory perception including pain and itch. Neurons in the ACC receive various neuromodulatory inputs from subcortical structures, including locus coeruleus noradrenaline (LC-NA) neurons. Few studies have been reported about synaptic and behavioral functions of LC-NA projections to the ACC. Using viral-genetic method (AAV-DIO-eYFP) on DBH-cre mice, we found that LC-NA formed synaptic connections to ACC pyramidal cells but not interneurons. This is further supported by the electron microscopic study showing NAergic fibers contact the presynaptic inputs and post-synaptic areas of the pyramidal cells. NA application produced both pre- and post-synaptic potentiation effects in ACC excitatory transmission in vivo and in vitro. Activation of LC-NA projection to the ACC by optogenetic method produced enhancement of excitatory transmission in vitro and induced scratching and behavioral sensitization for mechanical stimulation. Our results demonstrate that LC-NA projections enhance or facilitate brain responses to pain and itch by potentiating glutamatergic synaptic transmissions in the ACC.

scholarly journals Ascending noradrenergic excitation from the locus coeruleus to the anterior cingulate cortex

2020 ◽  
Author(s):  
Kohei Koga ◽  
Akihiro Yamada ◽  
Qian Song ◽  
Xu-Hui Li ◽  
Qi-Yu Chen ◽  
...  
Keyword(s):  
Locus Coeruleus ◽  
Anterior Cingulate Cortex ◽  
Behavioral Sensitization ◽  
Pyramidal Cells ◽  
Cingulate Cortex ◽  
Anterior Cingulate ◽  
Subcortical Structures ◽  
Electron Microscopic ◽  
Excitatory Transmission

Abstract Anterior cingulate cortex (ACC) plays important roles in sensory perception including pain and itch. Neurons in the ACC receive various neuromodulatory inputs from subcortical structures, including locus coeruleus noradrenaline (LC-NA) neurons. Few studies have been reported about synaptic and behavioral functions of LC-NA projections to the ACC. Using viral-genetic method (AAV-DIO-eYFP) on DBH-cre mice, we found that LC-NA formed synaptic connections to ACC pyramidal cells but not interneurons. This is further supported by the electron microscopic study showing NAergic fibers contact the presynaptic inputs and post-synaptic areas of the pyramidal cells. NA application produced both pre- and post-synaptic potentiation effects in ACC excitatory transmission in vivo and in vitro. Activation of LC-NA projection to the ACC by optogenetic method produced enhancement of excitatory transmission in vitro and induced scratching and behavioral sensitization for mechanical stimulation. Our results demonstrate that LC-NA projections enhance or facilitate brain responses to pain and itch by potentiating glutamatergic synaptic transmissions in the ACC.

scholarly journals Control of impulsivity by Gi-protein signalling in layer-5 pyramidal neurons of the anterior cingulate cortex

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Bastiaan van der Veen ◽  
Sampath K. T. Kapanaiah ◽  
Kasyoka Kilonzo ◽  
Peter Steele-Perkins ◽  
Martin M. Jendryka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Anterior Cingulate Cortex ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Pyramidal Neurons ◽  
Treatment Options ◽  
Pyramidal Cells ◽  
Cingulate Cortex ◽  
Cell Types ◽  
Anterior Cingulate

AbstractPathological impulsivity is a debilitating symptom of multiple psychiatric diseases with few effective treatment options. To identify druggable receptors with anti-impulsive action we developed a systematic target discovery approach combining behavioural chemogenetics and gene expression analysis. Spatially restricted inhibition of three subdivisions of the prefrontal cortex of mice revealed that the anterior cingulate cortex (ACC) regulates premature responding, a form of motor impulsivity. Probing three G-protein cascades with designer receptors, we found that the activation of Gi-signalling in layer-5 pyramidal cells (L5-PCs) of the ACC strongly, reproducibly, and selectively decreased challenge-induced impulsivity. Differential gene expression analysis across murine ACC cell-types and 402 GPCRs revealed that - among Gi-coupled receptor-encoding genes - Grm2 is the most selectively expressed in L5-PCs while alternative targets were scarce. Validating our approach, we confirmed that mGluR2 activation reduced premature responding. These results suggest Gi-coupled receptors in ACC L5-PCs as therapeutic targets for impulse control disorders.

scholarly journals Top-down descending facilitation of spinal sensory excitatory transmission from the anterior cingulate cortex

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Tao Chen ◽  
Wataru Taniguchi ◽  
Qi-Yu Chen ◽  
Hidetoshi Tozaki-Saitoh ◽  
Qian Song ◽  
...  
Keyword(s):  
Anterior Cingulate Cortex ◽  
Cingulate Cortex ◽  
Anterior Cingulate ◽  
Top Down ◽  
Excitatory Transmission ◽  
Descending Facilitation

scholarly journals NCAM Regulates Inhibition and Excitability in Layer 2/3 Pyramidal Cells of Anterior Cingulate Cortex

2017 ◽  
Vol 11 ◽  
Author(s):  
Xuying Zhang ◽  
Chelsea S. Sullivan ◽  
Megan B. Kratz ◽  
Michael R. Kasten ◽  
Patricia F. Maness ◽  
...  
Keyword(s):  
Anterior Cingulate Cortex ◽  
Pyramidal Cells ◽  
Cingulate Cortex ◽  
Anterior Cingulate ◽  
Layer 2

scholarly journals Anterior cingulate cortex connectivity is associated with suppression of behaviour in a rat model of chronic pain

2018 ◽  
Vol 2 ◽  
pp. 239821281877964 ◽  
Author(s):  
Laurel S. Morris ◽  
Christian Sprenger ◽  
Ken Koda ◽  
Daniela M. de la Mora ◽  
Tomomi Yamada ◽  
...  
Keyword(s):  
Anterior Cingulate Cortex ◽  
Cingulate Cortex ◽  
Persistent Pain ◽  
Anterior Cingulate ◽  
Subcortical Structures ◽  
Bed Nucleus ◽  
Motivational Aspect ◽  
Confirmatory Factor ◽  
Burrowing Behaviour ◽  
The Right

A cardinal feature of persistent pain that follows injury is a general suppression of behaviour, in which motivation is inhibited in a way that promotes energy conservation and recuperation. Across species, the anterior cingulate cortex is associated with the motivational aspects of phasic pain, but whether it mediates motivational functions in persistent pain is less clear. Using burrowing behaviour as an marker of non-specific motivated behaviour in rodents, we studied the suppression of burrowing following painful confirmatory factor analysis or control injection into the right knee joint of 30 rats (14 with pain) and examined associated neural connectivity with ultra-high-field resting state functional magnetic resonance imaging. We found that connectivity between anterior cingulate cortex and subcortical structures including hypothalamic/preoptic nuclei and the bed nucleus of the stria terminalis correlated with the reduction in burrowing behaviour observed following the pain manipulation. In summary, the findings implicate anterior cingulate cortex connectivity as a correlate of the motivational aspect of persistent pain in rodents.

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