Rapid Detection of Avian Leukosis Virus Subgroup J by Cross-priming Amplification

Avian leukosis virus subgroup J (ALV-J) causes oncogenic disease in chickens in China, resulting in great harm to poultry production. Herein, we employed a cross-priming amplification (CPA) approach and a nucleic acid detection device to establish a visual rapid detection method for ALV-J. When the amplification reaction was carried out at 60°C for just 60 min, the sensitivity of CPA was 10 times higher than conventional PCR, with high specificity, based on detection of 123 clinical plasma samples. The coincidence rate with real-time PCR was 97.3% (71/73). CPA detection of ALV-J does not require an expensive PCR instrument; a simple water bath or incubator is sufficient for complete DNA amplification, and the closed nucleic acid detection device avoids aerosol pollution, making judgment of results more intuitive and objective. The CPA assay would be a promising simple, rapid and sensitive method for identification of ALV-J.