Exercise intervention during pregnancy induces promoter methylation alterations in cord blood

Abstract Background: Environmental events and nutritional conditions may induce permanent DNA methylation changes in utero during the sensitive period and these adaptive changes may be ‘memorized’ and have a lasting impact on adult disease later in life. The potential plasticity of DNA methylation also enables reprogramming, depending on exposure to nutritional, chemical, and environmental factors. We determined the effects of exercise training during pregnancy on epigenetic modifications in offspring.Results: Twenty-four subjects were chosen from a previous clinical study. Overweight/obese pregnant women (body mass index <28/≥28kg/m2) who had an uncomplicated pregnancy at <12+6 weeks of gestation were randomly allocated to either an exercise or a control group. Patients allocated to the exercise group performed 3 exercise bouts per week (at least 30 min/session with a rating of perceived exertion between 12-14) via a cycling program that was initiated within 3 days of randomization until 37 weeks of gestation. Patients allocated to the control group continued their usual daily activities. Maternal blood, as well as umbilical cord blood samples, were collected and DNA methylation levels were determined by Illumina MethylationEPIC microarray. Nine CpG sites and corresponding genes UMAD1, RPA3, PLAGL2, POFUT1, SPATA17, GPATCH2, CEP170, MPHOSPH10, MCEE, as well as MRGPRD showed differential trends in control and exercise groups in maternal blood. Furthermore, four specific genes in umbilical cord blood were differentially methylated in the exercise group compared to the sedentary controls (p < 0.01). Conclusions: DNA methylation in maternal blood is dynamically altered during pregnancy and remodeled by exercise training. Thus, intrauterine environmental exposures play an important role in fetal programming. Epigenetic mechanisms involving changes in DNA methylation may provide an explanation for the phenomenon. Our findings shed light on understanding of long-term effects of in utero exposures on the epigenetic landscape of the offspring.

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Transcriptional Activity of Human Endogenous Retroviruses in Response to Prenatal Exposure of Maternal Cigarette Smoking

American Journal of Perinatology ◽

10.1055/s-0038-1675768 ◽

2018 ◽

Vol 36 (10) ◽

pp. 1060-1065 ◽

Cited By ~ 4

Author(s):

Massimiliano Bergallo ◽

Ilaria Galliano ◽

Valentina Daprà ◽

Alice Pirra ◽

Paola Montanari ◽

...

Keyword(s):

Dna Methylation ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Tobacco Smoke ◽

Transcriptional Activity ◽

In Utero ◽

Endogenous Retroviruses ◽

Human Endogenous Retrovirus ◽

Human Umbilical Cord

Objective Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and in DNA from cord blood. Study Design We assessed the transcriptional activity of HERV-H, HERV-K, and HERV-W in umbilical cord blood from 47 term babies unexposed to tobacco smoke in utero and 23 term babies exposed to tobacco smoke in utero. Results In our population, the HERV-H, HERV-K, and HERV-W families were always transcriptionally active, and the levels of all HERVs (H, K, W) were significantly higher in unexposed than smoke-exposed babies. Conclusion This study provides preliminary information about the transcriptional activity of HERV-H, HERV-K, and HERV-W families in human umbilical cord blood.

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Gestational Diabetes Mellitus in Pregnancy Increased Erythropoietin Level Affecting Differentiation Potency of Haematopoietic Stem Cell of Umbilical Cord Blood

Frontiers in Medicine ◽

10.3389/fmed.2021.727179 ◽

2021 ◽

Vol 8 ◽

Author(s):

Mohd Razif Mohd Idris ◽

Fazlina Nordin ◽

Zaleha Abdullah Mahdy ◽

S. Fadilah Abd Wahid

Keyword(s):

Diabetes Mellitus ◽

Stem Cell ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

In Utero ◽

Haematopoietic Stem Cell ◽

Control Group

Background: The in utero environment has many factors that can support cell differentiation. Cytokines, chemokines and growth factors play big roles in haematopoietic mechanisms. Some diseases like gestational diabetes mellitus (GDM) might affect the environment and haematopoietic stem cell (HSC) quality. The aim of this study is to investigate the adverse effects of GDM on umbilical cord blood (UCB) HSC in terms of differentiation potency including the UCB parameters used for banking and transplantation purposes.Methods: UCB-HSC was collected from 42 GDM and 38 normal pregnancies. UCB-HSC was isolated and further enriched using immuno-magnetic separation beads (MACS). The UCB-HSC were cultured in methylcellulose media to investigate the differentiation potency. The level of erythropoietin (EPO) and insulin in the UCB plasma was measured using enzyme linked immunoassay (ELISA) technique.Result: The UCB parameters; volume, total nucleated count (TNC) and total CD34+ cells were significantly reduced in the GDM group compared to the control group. The number of HSC progenitors' colonies were significantly reduced in the GDM group except for progenitor BFU-E, which was significantly increased (GDM = 94.19 ± 6.21, Control = 73.61 ± 2.73, p = 0.010). This data was associated with higher EPO level in GDM group. However, the insulin level in the GDM group was comparable to the Control group.Conclusion: Our results suggest that the changes in the in utero environment due to abnormalities during pregnancy such as GDM might affect the differentiation potency of UCB-HSC. These findings can be considered as an additional parameter for the inclusion and exclusion criteria for UCB banking, particularly for mothers with GDM.

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Gestational diabetes mellitus in pregnancy increased erythropoietin level affecting differentiation potency of haematopoietic stem cell of umbilical cord blood

10.21203/rs.3.rs-40849/v2 ◽

2020 ◽

Author(s):

Mohd Razif Mohd Idris ◽

Fazlina Nordin ◽

Zaleha Abdullah Mahdy ◽

S Fadilah Abd Wahid

Keyword(s):

Diabetes Mellitus ◽

Stem Cell ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

In Utero ◽

Haematopoietic Stem Cell ◽

Control Group

Abstract Background The in utero environment has many factors that can support cell differentiation. Cytokines, chemokines, and growth factors play big roles in haematopoietic mechanisms. Some diseases like gestational diabetes mellitus (GDM) might affect the environment and quality of haematopoietic stem cell (HSC). This study aims to investigate the adverse effects of GDM on umbilical cord blood (UCB) HSC regarding its differentiation potency, including the UCB parameters used for banking and transplantation purposes. Methods UCB-HSC was collected from 42 GDM and 38 normal pregnancies. UCB-HSC was isolated and further enriched using immuno-magnetic separation beads (MACS). The UCB-HSC were cultured in methylcellulose media to investigate the differentiation potency. The level of erythropoietin (EPO) and insulin in the UCB plasma was measured using enzyme-linked immunoassay (ELISA) technique. Results The UCB parameters, i.e., volume, total nucleated count (TNC), and total CD34+ cells, were significantly reduced in the GDM group compared to the control group. The number of HSC progenitor colonies was significantly reduced in the GDM group, except for progenitor BFU-E, which was significantly increased (GDM = 94.19 ± 6.21, Control = 73.61 ± 2.73, p = 0.010). This data was associated with a higher EPO level in the GDM group. However, the insulin level in the GDM group was comparable to the control group. Conclusion Our results suggest that the changes in the in utero environment due to abnormalities during pregnancy, such as GDM might affect the differentiation potency of UCB-HSC. These findings can be considered as an additional parameter for the inclusion and exclusion criteria for UCB banking, particularly for mothers with GDM.

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Influence Of Prenatal Mercury And Arsenic Exposure In Utero On Dna Methylation In Umbilical Cord Blood

ISEE Conference Abstracts ◽

10.1289/isee.2015.2015-703 ◽

2015 ◽

Vol 2015 (1) ◽

pp. 703

Author(s):

Andres Cardenas ◽

Devin C. Koestler ◽

E. Andres Houseman ◽

Brian P. Jackson ◽

Molly L. Kile ◽

...

Keyword(s):

Dna Methylation ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Arsenic Exposure ◽

In Utero

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Higher Levels of Triglyceride, Fatty Acid Translocase, and Toll-Like Receptor 4 and Lower Level of HDL-C in Pregnant Women with GDM and Their Close Correlation with Neonatal Weight

Gynecologic and Obstetric Investigation ◽

10.1159/000510032 ◽

2021 ◽

pp. 1-7

Author(s):

Jianli Zhou ◽

Jie Bai ◽

Yanjuan Guo ◽

Lijun Fu ◽

Jun Xing

Keyword(s):

Cord Blood ◽

Pregnant Women ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Venous Blood ◽

Density Lipoprotein ◽

Maternal Blood ◽

Control Group ◽

Case Group ◽

Toll Like Receptor 4

Objectives: In this study, we aimed to compare the levels of maternal blood lipids, placental and venous blood lipid transporters, and inflammatory factor receptors in pregnant women with and without gestational diabetes mellitus (GDM). We also aimed to figure out the relationship between these values and neonatal weight. Methods: Fifty pregnant women with GDM under blood glucose control belong to the case group, and 50 pregnant women with normal glucose tolerance in concurrent delivery belong to the control group. Fasting venous blood of these pregnant women was taken 2 weeks before delivery, and umbilical cord blood was collected after delivery. The levels of triglyceride (TG), serum total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol (HDL-C) in maternal blood and umbilical cord blood were tested in the laboratory department of our hospital. The level of toll-like receptor 4 (TLR4) in serum of umbilical veins was detected by the double-antibody sandwich ELISA. Western blot and RT-PCR were used to detect the protein and mRNA expressions of TLR4, LPL, and FAT/CD36 in the placenta. Results: The level of TG in maternal blood in the case group was remarkably higher than that in the control group, which was opposite to the level of HDL-C. In the umbilical cord blood of women with GDM, the expression of TLR4 increased and was closely correlated with neonatal weight. In the placenta of women with GDM, the expressions of FAT/CD36 and TLR4 increased, and both of them were closely correlated with neonatal weight. Besides, TLR4 in umbilical cord blood increased and was closely correlated with neonatal weight. Although the expression of LPL in the placenta decreased, it had no obvious correlation with neonatal weight. Conclusions: TG in maternal blood, TLR4 in the placenta and umbilical cord blood, and FAT/CD36 in the placenta were positively correlated with neonatal weight. However, HDL-C in maternal blood was negatively correlated with neonatal weight. Although the expression of LPL in the placenta reduced due to GDM, it had no correlation with neonatal weight.

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