Transcriptional Activity of Human Endogenous Retroviruses in Response to Prenatal Exposure of Maternal Cigarette Smoking

2018 ◽  
Vol 36 (10) ◽  
pp. 1060-1065 ◽  
Author(s):  
Massimiliano Bergallo ◽  
Ilaria Galliano ◽  
Valentina Daprà ◽  
Alice Pirra ◽  
Paola Montanari ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Tobacco Smoke ◽  
Transcriptional Activity ◽  
In Utero ◽  
Endogenous Retroviruses ◽  
Human Endogenous Retrovirus ◽  
Human Umbilical Cord

Objective Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and in DNA from cord blood. Study Design We assessed the transcriptional activity of HERV-H, HERV-K, and HERV-W in umbilical cord blood from 47 term babies unexposed to tobacco smoke in utero and 23 term babies exposed to tobacco smoke in utero. Results In our population, the HERV-H, HERV-K, and HERV-W families were always transcriptionally active, and the levels of all HERVs (H, K, W) were significantly higher in unexposed than smoke-exposed babies. Conclusion This study provides preliminary information about the transcriptional activity of HERV-H, HERV-K, and HERV-W families in human umbilical cord blood.

Influence Of Prenatal Mercury And Arsenic Exposure In Utero On Dna Methylation In Umbilical Cord Blood

2015 ◽  
Vol 2015 (1) ◽  
pp. 703
Author(s):  
Andres Cardenas ◽  
Devin C. Koestler ◽  
E. Andres Houseman ◽  
Brian P. Jackson ◽  
Molly L. Kile ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Arsenic Exposure ◽  
In Utero

Exercise intervention during pregnancy induces promoter methylation alterations in cord blood

2020 ◽  
Author(s):  
Jie Yan ◽  
Chen Wang ◽  
Yumei Wei ◽  
Huixia Yang
Keyword(s):  
Dna Methylation ◽  
Exercise Training ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Maternal Blood ◽  
In Utero ◽  
Exercise Group ◽  
Sensitive Period ◽  
Control Group

Abstract Background: Environmental events and nutritional conditions may induce permanent DNA methylation changes in utero during the sensitive period and these adaptive changes may be ‘memorized’ and have a lasting impact on adult disease later in life. The potential plasticity of DNA methylation also enables reprogramming, depending on exposure to nutritional, chemical, and environmental factors. We determined the effects of exercise training during pregnancy on epigenetic modifications in offspring.Results: Twenty-four subjects were chosen from a previous clinical study. Overweight/obese pregnant women (body mass index <28/≥28kg/m2) who had an uncomplicated pregnancy at <12+6 weeks of gestation were randomly allocated to either an exercise or a control group. Patients allocated to the exercise group performed 3 exercise bouts per week (at least 30 min/session with a rating of perceived exertion between 12-14) via a cycling program that was initiated within 3 days of randomization until 37 weeks of gestation. Patients allocated to the control group continued their usual daily activities. Maternal blood, as well as umbilical cord blood samples, were collected and DNA methylation levels were determined by Illumina MethylationEPIC microarray. Nine CpG sites and corresponding genes UMAD1, RPA3, PLAGL2, POFUT1, SPATA17, GPATCH2, CEP170, MPHOSPH10, MCEE, as well as MRGPRD showed differential trends in control and exercise groups in maternal blood. Furthermore, four specific genes in umbilical cord blood were differentially methylated in the exercise group compared to the sedentary controls (p < 0.01). Conclusions: DNA methylation in maternal blood is dynamically altered during pregnancy and remodeled by exercise training. Thus, intrauterine environmental exposures play an important role in fetal programming. Epigenetic mechanisms involving changes in DNA methylation may provide an explanation for the phenomenon. Our findings shed light on understanding of long-term effects of in utero exposures on the epigenetic landscape of the offspring.

IN UTERO EXPOSURE TO ARSENIC IS ASSOCIATED WITH ALTERED DNA METHYLATION IN UMBILICAL CORD BLOOD LEUKOCYTES

2011 ◽  
Vol 2011 (1) ◽  
Author(s):  
Molly L. Kile ◽  
Andrea Baccarelli ◽  
Elaine Hoffman ◽  
Letizia Tarantini ◽  
Quazi Quamruzzaman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
In Utero ◽  
In Utero Exposure ◽  
Blood Leukocytes

scholarly journals Exposure to Low Levels of Lead in Utero and Umbilical Cord Blood DNA Methylation in Project Viva: An Epigenome-Wide Association Study

2017 ◽  
Vol 125 (8) ◽  
pp. 087019 ◽  
Author(s):  
Shaowei Wu ◽  
Marie-France Hivert ◽  
Andres Cardenas ◽  
Jia Zhong ◽  
Sheryl L. Rifas-Shiman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cord Blood ◽  
Association Study ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
In Utero ◽  
Low Levels

Therapeutic Potential of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood

2019 ◽  
Vol 14 (6) ◽  
pp. 460-465 ◽  
Author(s):  
Jing Jia ◽  
Baitao Ma ◽  
Shaoshuai Wang ◽  
Ling Feng
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Tissue Regeneration ◽  
Umbilical Cord Blood ◽  
Therapeutic Potential ◽  
Human Umbilical Cord Blood ◽  
Proliferative Potential ◽  
Human Umbilical Cord ◽  
Preclinical Studies ◽  
Endothelial Colony Forming Cells

Endothelial progenitor cells (EPCs) are implicated in multiple biologic processes such as vascular homeostasis, neovascularization and tissue regeneration, and tumor angiogenesis. A subtype of EPCs is referred to as endothelial colony-forming cells (ECFCs), which display robust clonal proliferative potential and can form durable and functional blood vessels in animal models. In this review, we provide a brief overview of EPCs’ characteristics, classification and origins, a summary of the progress in preclinical studies with regard to the therapeutic potential of human umbilical cord blood derived ECFCs (CB-ECFCs) for ischemia repair, tissue engineering and tumor, and highlight the necessity to select high proliferative CB-ECFCs and to optimize their recovery and expansion conditions.

Hoechst dye efflux reveals a novel CD7+CD34− lymphoid progenitor in human umbilical cord blood

Blood ◽  
2000 ◽  
Vol 96 (6) ◽  
pp. 2125-2133 ◽  
Author(s):  
Robert W. Storms ◽  
Margaret A. Goodell ◽  
Alan Fisher ◽  
Richard C. Mulligan ◽  
Clay Smith
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Large Population ◽  
Lymphoid Cells ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Hematopoietic Stem ◽  
Cd34 Cells

Abstract A novel Hoechst 33342 dye efflux assay was recently developed that identifies a population of hematopoietic cells termed side population (SP) cells. In the bone marrow of multiple species, including mice and primates, the SP is composed primarily of CD34−cells, yet has many of the functional properties of hematopoietic stem cells (HSCs). This report characterizes SP cells from human umbilical cord blood (UCB). The SP in unfractionated UCB was enriched for CD34+ cells but also contained a large population of CD34− cells, many of which were mature lymphocytes. SP cells isolated from UCB that had been depleted of lineage-committed cells (Lin− UCB) contained CD34+ and CD34− cells in approximately equivalent proportions. Similar to previous descriptions of human HSCs, the CD34+Lin− SP cells were CD38dimHLA-DRdimThy-1dimCD45RA−CD71−and were enriched for myelo-erythroid precursors. In contrast, the CD34−Lin− SP cells were CD38−HLA-DR−Thy-1−CD71−and failed to generate myelo-erythroid progeny in vitro. The majority of these cells were CD7+CD11b+CD45RA+, as might be expected of early lymphoid cells, but did not express other lymphoid markers. The CD7+CD34−Lin− UCB SP cells did not proliferate in simple suspension cultures but did differentiate into natural killer cells when cultured on stroma with various cytokines. In conclusion, the human Lin− UCB SP contains both CD34+ multipotential stem cells and a novel CD7+CD34−Lin− lymphoid progenitor. This observation adds to the growing body of evidence that CD34− progenitors exist in humans.

scholarly journals Endothelial cell precursors are normal components of human umbilical cord blood

1997 ◽  
Vol 98 (3) ◽  
pp. 775-777 ◽  
Author(s):  
Mie Nieda ◽  
Andrew Nicol ◽  
Patricia Denning‐Kendall ◽  
John Sweetenham ◽  
Ben Bradley ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord
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