scholarly journals The VetMAXTM M. tuberculosis Complex PCR Kit detects MTBC DNA in antemortem and postmortem samples from white rhinoceros (Ceratotherium simum), African elephants (Loxodonta africana) and African buffaloes (Syncerus caffer)

2020 ◽  
Author(s):  
Wynand Johan Goosen ◽  
Tanya Jane Kerr ◽  
Léanie Kleynhans ◽  
Peter Buss ◽  
David Cooper ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
African Elephants ◽  
Tuberculosis Complex ◽  
Pcr Assay ◽  
Wildlife Species ◽  
Exposed Population ◽  
White Rhinoceros ◽  
Polymerase Chain ◽  
Positive Results ◽  
Culture Negative

Abstract Background: Bovine tuberculosis and tuberculosis are chronic infectious diseases caused by the Mycobacterium tuberculosis complex members, Mycobacterium bovis and Mycobacterium tuberculosis, respectively. Infection with M. bovis and M. tuberculosis have significant implications for wildlife species management, public health, veterinary disease control, and conservation endeavours.Results: Here we describe the first use of the VetMAXTM Mycobacterium tuberculosis complex (MTBC) DNA quantitative real-time polymerase chain reaction (qPCR) detection kit for wildlife samples. DNA was extracted from tissues harvested from 48 African buffaloes and MTBC DNA was detected (test-positive) in all 26 M. bovis culture-confirmed animals with an additional 12 PCR-positive results in culture-negative buffaloes (originating from an exposed population). Of six MTBC-infected African rhinoceros tested, MTBC DNA was detected in antemortem and postmortem samples from five animals. The PCR was also able to detect MTBC DNA in samples from two African elephants confirmed to have M. bovis and M. tuberculosis infections (one each). Culture-confirmed uninfected rhinoceros and elephants’ samples tested negative in the PCR assay.Conclusions: These results suggest this new detection kit is a sensitive screening test for the detection of MTBC-infected African buffaloes, African elephants and white rhinoceros.

scholarly journals The VetMAXTM M. tuberculosis Complex PCR Kit detects MTBC DNA in antemortem and postmortem samples from White rhinoceros (Ceratotherium simum), African elephants (Loxodonta africana) and African buffaloes (Syncerus caffer).

2020 ◽  
Author(s):  
Wynand Johan Goosen ◽  
Tanya Jane Kerr ◽  
Léanie Kleynhans ◽  
Peter Buss ◽  
David Cooper ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
African Elephants ◽  
Tuberculosis Complex ◽  
Wildlife Species ◽  
Exposed Population ◽  
White Rhinoceros ◽  
Polymerase Chain ◽  
Positive Results ◽  
Culture Negative

Abstract Background: Bovine tuberculosis and tuberculosis are chronic infectious diseases caused by the Mycobacterium tuberculosis complex members, Mycobacterium bovis and Mycobacterium tuberculosis , respectively. Infection with M. bovis and M. tuberculosis have significant implications for wildlife species management, public health, veterinary disease control, and conservation endeavours. Results : Here we describe the first use of the VetMAX TM Mycobacterium tuberculosis complex (MTBC) DNA quantitative real-time polymerase chain reaction (qPCR) detection kit for African wildlife samples. DNA was extracted from tissues harvested from 48 African buffaloes and MTBC DNA was detected (test-positive) in all 26 M. bovis culture-confirmed animals with an additional 12 PCR-positive results in culture-negative buffaloes (originating from an exposed population). Of six MTBC-infected African rhinoceros tested, MTBC DNA was detected in antemortem and postmortem samples from five animals. The PCR was also able to detect MTBC DNA in samples from two African elephants confirmed to have M. bovis and M. tuberculosis infections (one each). Culture-confirmed uninfected rhinoceros and elephants’ samples tested negative in the PCR assay.Conclusions: These results suggest this new detection kit is a sensitive screening test for the detection of MTBC-infected African buffaloes, African elephants and white rhinoceros.

scholarly journals Evaluation of the COBAS AMPLICOR MTB test for the detection of Mycobacterium tuberculosis complex

2004 ◽  
Vol 10 (3) ◽  
pp. 329-335
Author(s):  
K. K. Abu Amero ◽  
M. A. Halablab
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex ◽  
False Negatives ◽  
Predictive Values ◽  
Lowenstein Jensen ◽  
Polymerase Chain ◽  
Sensitivity Specificity ◽  
Culture Positive ◽  
Positive Results

Wevaluated the COBAS AMPLICOR polymerase chain reaction [PCR] based test for the detection of Mycobacterium tuberculosis complex in 866 respiratory and non-respiratory samples. Acid-fast staining and culture on Lowenstein-Jensen medium were also performed on all samples. Of the 866 samples tested, 87 [10.0%] were PCR-positive compared to 94 [10.9%] culture positive. There were no false positive results but 7 PCR-negative, culture-positive samples were, considered false negatives after reviewing medical records of patients. A PCR inhibitory rate of 2.0% [17/866] was observed in respiratory samples only. Sensitivity, specificity, and positive and negative predictive values for this test were 92.5%, 100%, 100% and 99.1% respectively. This test is a valuable diagnostic tool for today’s mycobacteriology laboratory

scholarly journals Detection of Mycobacterium tuberculosis Complex Bacilli and Nucleic Acids From Tongue Swabs in Young, Hospitalized Children

2021 ◽  
Vol 11 ◽  
Author(s):  
Christopher Ealand ◽  
Julian Peters ◽  
Olivia Jacobs ◽  
Astika Sewcharran ◽  
Azra Ghoor ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Genomic Dna ◽  
Genetic Material ◽  
Smear Microscopy ◽  
Hospitalized Children ◽  
Tuberculosis Complex ◽  
Tuberculosis In Children ◽  
Positive Results ◽  
Culture Negative ◽  
Oral Epithelia

Diagnosis of tuberculosis in pediatric patients remains challenging due to inherent difficulties associated with obtaining respiratory samples for molecular and culture-based testing. To address this, recent studies have highlighted the utility of tongue swabs to detect Mycobacterium tuberculosis genomic DNA in the oral epithelia of tuberculosis infected adults. It is unknown whether tongue swabs have similar utility for diagnosis of childhood tuberculosis and if the presence of DNA in these swabs was associated with whole bacilli. We therefore sought to conduct a preliminary assessment of the utility of tongue swabs to detect tubercle bacilli and their associated genetic material in young children. For this, we recruited hospitalized children with clinically diagnosed tuberculosis (n = 26) or lower respiratory tract infection (LRTI, n = 9). These categories were blinded for downstream laboratory tests, which included PCR, spoligotyping, smear microscopy, and culture. Mtb genomic DNA was detected by PCR only in clinically diagnosed TB cases [11/26 (31.4%)] and not in cases with LRTI. Of these, 5/11 [45.5%] were associated with a spoligotype. Spoligotyping also detected an additional six specimens that were negative by PCR. Using smear microscopy, 19/26 [73.1%] and 4/9 [44.4] were Mtb positive in the tuberculosis or LRTI categories respectively. We noted positive results on all three tests in 5/26 [19.2%] in the tuberculosis category and 0/9 in the LRTI category. All specimens were culture negative. Collectively, these preliminary data present a compelling case for broader testing of tongue swabs to diagnose tuberculosis in children where obtaining standard sputum specimens is not easy.

DETECTING MYCOBACTERIUM TUBERCULOSIS RAPIDLY AND MYCOBACTERIUM TUBERCULOSIS STRAINS WITHOUT IS6110 SEQUENCE BY PCR ASSAY

2012 ◽  
pp. 15-19
Author(s):  
Thi Chau Anh Nguyen ◽  
Hoang Bach Nguyen ◽  
Hai Duong Huynh ◽  
Nu Xuan Thanh Le ◽  
Xuan Cuong Le ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
16S Rdna ◽  
False Negative ◽  
Clinical Samples ◽  
Tuberculosis Complex ◽  
Pcr Assay ◽  
Complex Objects ◽  
Method Performance ◽  
Gene Coding ◽  
16S Rdna Pcr

Background: The Nested IS6110 PCR is used for detecting tuberculosis, however IS6110 sequence is not present in the genome of all strains of M.tuberculosis, the result may be false negative. The gene coding 16S ribosome always contains a short sequence specific to M. tuberculosis complex. Objects: Performance of the 16S Real-time PCR to detect M. tuberculosis and combining to the nested IS6110 PCR to determine the rate of Mtb strains without IS6110 from clinical samples. Materials and method: Performance of 16S rDNA PCR by commercial kit of Viet A Inc. for all 480 samples, the samples which were positive with the 16S rDNA PCR were retested in IS6110 PCR assay by in-house kit. Results: The Realtime 16S rDNA PCR detected 258 cases (53.8%) of tuberculosis. There were 3 (1.2 %) M. tuberculosis strains which do not harbor IS6110 sequence in genome. Conclusion: The IS6110 nested PCR can be applied more widely than the 16S rDNA realtime PCR. In case of using IS6110 PCR assay, results may show a low proportion of false negative. Combining 16S rDNA PCR with the IS6110 based PCR allowed detection of deletion of IS6110 sequence in M. tuberculosis isolates.

scholarly journals Severe pneumonia after intravesical BCG instillation in a patient with invasive bladder cancer: case report and literature review

2015 ◽  
Vol 79 (1) ◽  
Author(s):  
G. Caramori ◽  
D. Artioli ◽  
G. Ferrara ◽  
R. Cazzuffi ◽  
C. Pasquini ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis Complex ◽  
Severe Pneumonia ◽  
Intravesical Instillation ◽  
Community Acquired Pneumonia ◽  
Cancer Case ◽  
Tuberculosis Complex ◽  
Pcr Assay ◽  
Positive Polymerase Chain Reaction ◽  
Bladder Cancer Case ◽  
Polymerase Chain

We present here the case of a 66 year old man with a severe bilateral community acquired pneumonia secondary to dissemination after an intravesical instillation of bacilllus Calmette-Guérin (BCG). Diagnosis was based on positive polymerase chain reaction (PCR) for mycobacterium tuberculosis complex in bronchoalveolar lavage and on the finding on transbronchial biopsy of non necrotising granulomas histopathologically similar to the granulomas found in bladder biopsies. These findings were confirmed using a validated real time PCR assay demonstrating the presence of the BCG genome in transbronchial and bladder biopsies.

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