scholarly journals Culture of IMR90 Cells in Advanced MEM with Reduced Serum

2021 ◽  
Author(s):  
Nicholas M. Mallek ◽  
Shaun D. McCullough
Keyword(s):  
Environmental Protection Agency ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Protection Agency ◽  
Human Lung Fibroblast ◽  
Lung Fibroblast Cell Line ◽  
The Us ◽  
Scope Of Application ◽  
Trade Names

Abstract NOTE: Methods document and worksheet versions of this method are attached as PDFs.SCOPE OF APPLICATION (LIMITATIONS)This method describes the thawing, culturing, and cryopreservation of the human lung fibroblast cell line IMR90 in Advanced MEM-based growth medium. Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Culture of IMR90 Cells

2020 ◽  
Author(s):  
Shaun D. McCullough
Keyword(s):  
Environmental Protection Agency ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Protection Agency ◽  
Human Lung Fibroblast ◽  
Plating Density ◽  
Lung Fibroblast Cell Line ◽  
The Us ◽  
Trade Names ◽  
Cell Plating

Abstract This protocol describes the thawing, culturing, and cryopreservation of the human lung fibroblast cell line IMR90. The attached methods document is a formal version of the information included here. The attached worksheet is a fillable PDF that can be used to maintain cell passage records using this protocol. Please note: Deviation from the three-day passage cycle and cell plating density described here typically results in greater culture and experimental variability.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

scholarly journals Essential Oil Content of the Rhizome ofCurcuma purpurascensBl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sok-Lai Hong ◽  
Guan-Serm Lee ◽  
Syarifah Nur Syed Abdul Rahman ◽  
Omer Abdalla Ahmed Hamdi ◽  
Khalijah Awang ◽  
...  
Keyword(s):  
Human Lung ◽  
Carcinoma Cell ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Fibroblast Cell Line ◽  
Human Carcinoma ◽  
Human Lung Fibroblast ◽  
Carcinoma Cell Lines ◽  
Lung Fibroblast Cell Line ◽  
Human Carcinoma Cell

Curcuma purpurascensBl., belonging to the Zingiberaceae family, is known astemu tisin Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Ski, A549, HT29, and HCT116) and a normal human lung fibroblast cell line (MRC5). Results from GC-MS and GC-FID analysis of the rhizome oil oftemu tisshowed turmerone as the major component, followed by germacrone,ar-turmerone, germacrene-B, and curlone. The rhizome oil oftemu tisexhibited strong cytotoxicity against HT29 cells (IC50value of 4.9 ± 0.4 μg/mL), weak cytotoxicity against A549, Ca Ski, and HCT116 cells (with IC50values of 46.3 ± 0.7, 32.5 ± 1.1, and 35.0 ± 0.3 μg/mL, resp.), and no inhibitory effect against MCF7 cells. It exhibited mild cytotoxicity against a noncancerous human lung fibroblast cell line (MRC5), with an IC50value of 25.2 ± 2.7 μg/mL. This is the first report on the chemical composition of this rhizome’s oil and its selective antiproliferative effect on HT29. The obtained data provided a basis for further investigation of the mode of cell death.

Histamine and Serotonin Stimulate Eotaxin Production by a Human Lung Fibroblast Cell Line

2002 ◽  
Vol 128 (Suppl. 1) ◽  
pp. 12-17 ◽  
Author(s):  
Etsuro Sato ◽  
Masayuki Haniuda ◽  
Hiroki Numanami ◽  
Toshiki Ushiyama ◽  
Akihiro Tsukadaira ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Fibroblast Cell Line ◽  
Human Lung Fibroblast ◽  
Lung Fibroblast Cell Line

scholarly journals Culture and Differentiation of Primary Human Tracheobronchial Epithelial Cells Using STEMCELL Technologies Pneumacult Media

2021 ◽  
Author(s):  
Lisa A. Dailey ◽  
Shaun D. McCullough
Keyword(s):  
Epithelial Cells ◽  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Protection Agency ◽  
Commercial Products ◽  
Brush Biopsy ◽  
Previous Version ◽  
The Us ◽  
Scope Of Application ◽  
Trade Names

Abstract NOTE: A PDF methods document is attached in the supplementary materials.SCOPE OF APPLICATION (LIMITATIONS)This method describes the culture and differentiation of primary human tracheobronchial epithelial cells (pHBEC). Cells used for this method can be obtained by brush biopsy during clinical bronchoscopy or purchased commercially. This method replaces the previous version described in (Dailey and McCullough, 2021a; b). Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Bradykinin stimulates eotaxin production by a human lung fibroblast cell line

2000 ◽  
Vol 106 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Etsuro Sato ◽  
Dan K. Nelson ◽  
Sekiya Koyama ◽  
Jeffrey C. Hoyt ◽  
Richard A. Robbins
Keyword(s):  
Cell Line ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Fibroblast Cell Line ◽  
Human Lung Fibroblast ◽  
Lung Fibroblast Cell Line
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