Culture of IMR90 Cells

2020 ◽  
Author(s):  
Shaun D. McCullough
Keyword(s):  
Environmental Protection Agency ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Protection Agency ◽  
Human Lung Fibroblast ◽  
Plating Density ◽  
Lung Fibroblast Cell Line ◽  
The Us ◽  
Trade Names ◽  
Cell Plating

Abstract This protocol describes the thawing, culturing, and cryopreservation of the human lung fibroblast cell line IMR90. The attached methods document is a formal version of the information included here. The attached worksheet is a fillable PDF that can be used to maintain cell passage records using this protocol. Please note: Deviation from the three-day passage cycle and cell plating density described here typically results in greater culture and experimental variability.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

scholarly journals Culture of IMR90 Cells in Advanced MEM with Reduced Serum

2021 ◽  
Author(s):  
Nicholas M. Mallek ◽  
Shaun D. McCullough
Keyword(s):  
Environmental Protection Agency ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Protection Agency ◽  
Human Lung Fibroblast ◽  
Lung Fibroblast Cell Line ◽  
The Us ◽  
Scope Of Application ◽  
Trade Names

Abstract NOTE: Methods document and worksheet versions of this method are attached as PDFs.SCOPE OF APPLICATION (LIMITATIONS)This method describes the thawing, culturing, and cryopreservation of the human lung fibroblast cell line IMR90 in Advanced MEM-based growth medium. Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Culture of 16HBE14o- Cells

2020 ◽  
Author(s):  
Nicole A. McNabb ◽  
Shaun D. McCullough
Keyword(s):  
Environmental Protection Agency ◽  
Bronchial Epithelial Cell ◽  
Epithelial Cell Line ◽  
Cell Passage ◽  
Protection Agency ◽  
Bronchial Epithelial ◽  
Plating Density ◽  
The Us ◽  
Trade Names ◽  
Cell Plating

Abstract This protocol describes the thawing, culturing, and cryopreservation of the human bronchial epithelial cell line 16HBE14o- (referred to as “16HBE”). The attached methods document is a formal version of the information included here. The attached worksheet is a fillable PDF that can be used to maintain cell passage records using this protocol.Please note: Deviation from the three-day passage cycle and cell plating density described here typically results in greater culture and experimental variability.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

scholarly journals Essential Oil Content of the Rhizome ofCurcuma purpurascensBl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sok-Lai Hong ◽  
Guan-Serm Lee ◽  
Syarifah Nur Syed Abdul Rahman ◽  
Omer Abdalla Ahmed Hamdi ◽  
Khalijah Awang ◽  
...  
Keyword(s):  
Human Lung ◽  
Carcinoma Cell ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Fibroblast Cell Line ◽  
Human Carcinoma ◽  
Human Lung Fibroblast ◽  
Carcinoma Cell Lines ◽  
Lung Fibroblast Cell Line ◽  
Human Carcinoma Cell

Curcuma purpurascensBl., belonging to the Zingiberaceae family, is known astemu tisin Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Ski, A549, HT29, and HCT116) and a normal human lung fibroblast cell line (MRC5). Results from GC-MS and GC-FID analysis of the rhizome oil oftemu tisshowed turmerone as the major component, followed by germacrone,ar-turmerone, germacrene-B, and curlone. The rhizome oil oftemu tisexhibited strong cytotoxicity against HT29 cells (IC50value of 4.9 ± 0.4 μg/mL), weak cytotoxicity against A549, Ca Ski, and HCT116 cells (with IC50values of 46.3 ± 0.7, 32.5 ± 1.1, and 35.0 ± 0.3 μg/mL, resp.), and no inhibitory effect against MCF7 cells. It exhibited mild cytotoxicity against a noncancerous human lung fibroblast cell line (MRC5), with an IC50value of 25.2 ± 2.7 μg/mL. This is the first report on the chemical composition of this rhizome’s oil and its selective antiproliferative effect on HT29. The obtained data provided a basis for further investigation of the mode of cell death.

Trans-Epithelial Exposure Model (TEEM) - Epithelial Cells and Fibroblasts

2020 ◽  
Author(s):  
Shaun D. McCullough
Keyword(s):  
Particulate Matter ◽  
Epithelial Cells ◽  
Cell Line ◽  
Environmental Protection Agency ◽  
Airway Epithelium ◽  
Exposure Model ◽  
Protection Agency ◽  
The Us ◽  
Trade Names

Abstract This exposure model is meant to serve as an in vitro representation of trans-epithelial effects of airway diesel and particulate matter exposures on fibroblasts in the stroma. The epithelial cells in the airway epithelium are represented by 16HBE cells and the fibroblasts are represented by either the IMR90 cell line or primary fibroblasts.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

LIVE/DEAD™ Fixable Near-IR Cell Viability Assay

2020 ◽  
Author(s):  
Samantha C. Faber ◽  
Shaun McCullough
Keyword(s):  
Cell Viability ◽  
Environmental Protection Agency ◽  
Protection Agency ◽  
Cell Viability Assay ◽  
Antibody Staining ◽  
Viability Assay ◽  
Near Ir ◽  
The Us ◽  
Trade Names ◽  
Formal Version

Abstract This protocol is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Measurement of Trans-Epithelial Electrical Resistance with EVOM2 and Chopstick Electrode

2020 ◽  
Author(s):  
Shaun D. McCullough
Keyword(s):  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Electrical Resistance ◽  
Cell Monolayer ◽  
Protection Agency ◽  
Commercial Products ◽  
The Us ◽  
Trade Names ◽  
Agency Policy

Abstract Trans-epithelial Electrical Resistance (TEER) can be used as a measure of cell monolayer confluence, health, and integrity. An Epithelial Voltohmmeter (EVOM) is used to take TEER measurements. This method details how to take TEER readings using an EVOM with chopstick electrode.Please note that chopstick electrodes are only designed for use with 12 mm inserts.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Histamine and Serotonin Stimulate Eotaxin Production by a Human Lung Fibroblast Cell Line

2002 ◽  
Vol 128 (Suppl. 1) ◽  
pp. 12-17 ◽  
Author(s):  
Etsuro Sato ◽  
Masayuki Haniuda ◽  
Hiroki Numanami ◽  
Toshiki Ushiyama ◽  
Akihiro Tsukadaira ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Fibroblast Cell Line ◽  
Human Lung Fibroblast ◽  
Lung Fibroblast Cell Line
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