scholarly journals A detailed and radioisotope-free protocol for electrophoretic mobility shift assay (EMSA)

2021 ◽  
Author(s):  
NGUYEN HOAI NGUYEN
Keyword(s):  
Transcription Factor ◽  
Electrophoretic Mobility Shift Assay ◽  
Electrophoretic Mobility ◽  
Chromatin Immunoprecipitation ◽  
Direct Interaction ◽  
Luciferase Reporter ◽  
Mobility Shift ◽  
Dna Fragment ◽  
Mobility Shift Assay

Abstract To comprehensively characterize the functions of a transcription factor (TF), it is required to analyze the interaction of this TF with its targeted loci. Several methods such as β-glucuronidase (GUS) or luciferase reporter, yeast one-hybrid (Y1H), chromatin-immunoprecipitation (ChIP), and electrophoretic mobility shift assay (EMSA) assays have been developed. Of these, EMSA is an in vitro method which can prove the direct interaction between TF and targeted DNA fragment. This protocol is to provide a detailed procedure for a safe EMSA assay (without using any radioisotope).

Transcription factor binding study by capillary zone electrophoretic mobility shift assay

2003 ◽  
Vol 24 (12) ◽  
pp. 96-100 ◽  
Author(s):  
Zsolt Ronai ◽  
Yan Wang ◽  
Julia Khandurina ◽  
Paul Budworth ◽  
Maria Sasvari-Szekely ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Electrophoretic Mobility Shift Assay ◽  
Electrophoretic Mobility ◽  
Transcription Factor Binding ◽  
Binding Study ◽  
Mobility Shift ◽  
Factor Binding ◽  
Capillary Zone ◽  
Mobility Shift Assay

Rapid qualitative evaluation of DNA transcription factor NF-κB by microchip electrophoretic mobility shift assay in mammalian cells

2011 ◽  
Vol 32 (22) ◽  
pp. 3241-3247 ◽  
Author(s):  
Sonoko Inoue ◽  
Noritada Kaji ◽  
Masatoshi Kataoka ◽  
Yasuo Shinohara ◽  
Yukihiro Okamoto ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Electrophoretic Mobility Shift Assay ◽  
Electrophoretic Mobility ◽  
Mammalian Cells ◽  
Qualitative Evaluation ◽  
Mobility Shift ◽  
Dna Transcription ◽  
Mobility Shift Assay

Capillary electrophoretic mobility shift assay for binding of DNA with NFAT3, a transcription factor from H9c2 cardiac myoblast cells

2011 ◽  
Vol 32 (16) ◽  
pp. 2174-2180 ◽  
Author(s):  
Soo Hyun Park ◽  
Eunmi Ban ◽  
Eun Joo Song ◽  
Hyunjung Lee ◽  
Doo Soo Chung ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Electrophoretic Mobility Shift Assay ◽  
Electrophoretic Mobility ◽  
Mobility Shift ◽  
Capillary Electrophoretic ◽  
Mobility Shift Assay ◽  
Myoblast Cells
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