scholarly journals Description of a Rat Model of Polymicrobial Abdominal Sepsis Mimicking Human Colon Perforation

2020 ◽  
Author(s):  
Julia M Utiger ◽  
Michael Glas ◽  
Anja Levis ◽  
Josef Prazak ◽  
Matthias Haenggi
Keyword(s):  
Blood Pressure ◽  
Surgical Repair ◽  
Cecal Ligation ◽  
Human Colon ◽  
Abdominal Sepsis ◽  
Colon Perforation ◽  
Cecal Ligation And Puncture ◽  
Objective Standard ◽  
Colon Ascendens ◽  
Peritonitis Model

Abstract Objective: Standard rodent sepsis models as cecal ligation and puncture models (CLP) or cecal ligation and incision models (CLI) are frequently not suited experiments, mainly because they lack surgical repair, and they are difficult to control for severity. The colon ascendens stent peritonitis model (CASP) overcomes some of these limitations. Result: Here we present our modification of the rodent CASP model, where severity of sepsis can be controlled by timing of surgical repair and treatment, and by diameter of the stent. Further, basic hemodynamic monitoring (blood pressure and heart rate) and frequent blood sampling can be achieved, which might guide further treatment.

scholarly journals Description of a rat model of polymicrobial abdominal sepsis mimicking human colon perforation

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Julia M. Utiger ◽  
Michael Glas ◽  
Anja Levis ◽  
Josef Prazak ◽  
Matthias Haenggi
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Surgical Repair ◽  
Cecal Ligation ◽  
Human Colon ◽  
Abdominal Sepsis ◽  
Colon Perforation ◽  
Cecal Ligation And Puncture ◽  
Colon Ascendens ◽  
Peritonitis Model

Abstract Objective Standard rodent sepsis models as cecal ligation and puncture models (CLP) or cecal ligation and incision models (CLI) are frequently not suited experiments, mainly because they lack surgical repair, and they are difficult to control for severity. The colon ascendens stent peritonitis model (CASP) overcomes some of these limitations. Result Here we present our modification of the rodent CASP model, where severity of sepsis can be controlled by timing of surgical repair and treatment, and by diameter of the stent. Further, basic hemodynamic monitoring (blood pressure and heart rate) and frequent blood sampling can be achieved, which might guide further treatment.

scholarly journals Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 100-105 ◽  
Author(s):  
Silvana Marques e Silva ◽  
Marcos Vinícius Melo de Oliveira ◽  
Alexandre Malta Brandão ◽  
Fabiana Pirani Carneiro ◽  
Vânia Maria Moraes Ferreira ◽  
...  
Keyword(s):  
Adhesion Formation ◽  
Cecal Ligation ◽  
Abdominal Sepsis ◽  
Colon Anastomosis ◽  
Cecal Ligation And Puncture ◽  
Strength Assessment ◽  
The Third ◽  
Peritoneal Infection ◽  
Histopathologic Analysis

PURPOSE: To evaluate the effects of abdominal sepsis on adhesion formation and colon anastomosis healing in rats. METHODS: Forty rats were distributed in two groups containing 20 rats each for left colon anastomosis in the presence (Group S) or absence (Group N) of induced sepsis by cecal ligation and puncture. Each group was divided into subgroups for euthanasia on the third (N3 and S3) or seventh (N7 or S7) post-operative day. The amount of adhesions was evaluated and a segment of the colon was removed for histopathologic analysis, bursting strength assessment, hydroxyproline and the determination of tissue collagen. RESULTS: The subjects which underwent cecal ligation and puncture presented a higher amount of intra-abdominal adherences in both third (p=0,00) and seventh (p=0,00) post-operatory days. Smaller bursting strengths were found in the S3 subgroup, and greater bursting strengths were found in the S7 subgroup. There was no difference in the variations on the concentrations of hydroxyproline, tissue collagen and histopathology. CONCLUSIONS: The peritoneal infection which was developed by cecal ligation and puncture raised the amount of intra-cavitary adhesions. There was a decrease in the amount of colonic anastomosis on the third post-operatory day with a following raise on the seventh without any effects on other healing parameters.

scholarly journals Sepsis-induced acute kidney injury: kidney protection effects by antioxidants

2018 ◽  
Vol 71 (4) ◽  
pp. 1921-1927 ◽  
Author(s):  
Carolina Ferreira Vasco ◽  
Mirian Watanabe ◽  
Cassiane Dezoti da Fonseca ◽  
Maria de Fátima Fernandes Vattimo
Keyword(s):  
Blood Pressure ◽  
Acute Kidney Injury ◽  
Creatinine Clearance ◽  
Experimental Model ◽  
Kidney Function ◽  
Cecal Ligation ◽  
Kidney Injury ◽  
Cecal Ligation And Puncture ◽  
Antioxidant Action ◽  
Average Blood Pressure

ABSTRACT Objective: To evaluate the antioxidant action of N-acetylcysteine and diosmin-hesperidin in an experimental model of sepsis-induced acute kidney injury in rats. Methods: The study used 20 Wistar adult male rats divided into the following groups: control (laparotomy with no induction of abdominal sepsis), sepsis (experimental model of sepsis with cecal ligation and puncture), N-acetylcysteine + sepsis and diosmin-hesperidin + sepsis. The evaluation contemplated physiological parameters (temperature, glycemia, and average blood pressure), kidney function (creatinine clearance), oxidative stress (urinary peroxides) and kidney histology. Results: The animals submitted to cecal ligation and puncture (sepsis) presented lower body temperature, lower average blood pressure, reduced creatinine clearance and increased urinary hydrogen peroxide levels. Treatment with diosmin-hesperidin improved kidney function and led to a reduction in the excretion of oxidative metabolites. Conclusion: The present study highlighted the protective antioxidant action of diosmin-hesperidin in the experimental model of sepsis-induced acute kidney injury.

CECAL LIGATION AND PUNCTURE VERSUS COLON ASCENDENS STENT PERITONITIS: TWO DISTINCT ANIMAL MODELS FOR POLYMICROBIAL SEPSIS

Shock ◽  
2004 ◽  
Vol 21 (6) ◽  
pp. 505-512 ◽  
Author(s):  
Stefan Maier ◽  
Tobias Traeger ◽  
Markus Entleutner ◽  
Alexandra Westerholt ◽  
Britta Kleist ◽  
...  
Keyword(s):  
Animal Models ◽  
Cecal Ligation ◽  
Polymicrobial Sepsis ◽  
Cecal Ligation And Puncture ◽  
Colon Ascendens

Cecal Ligation and Puncture Peritonitis Model Shows Decreased Nicotinic Acetylcholine Receptor Numbers in Rat Muscle 

1999 ◽  
Vol 91 (2) ◽  
pp. 448-460 ◽  
Author(s):  
Hiroshi Tsukagoshi ◽  
Toshihiro Morita ◽  
Kenichiro Takahashi ◽  
Fumio Kunimoto ◽  
Fumio Goto
Keyword(s):  
Systemic Inflammation ◽  
Nicotinic Acetylcholine Receptors ◽  
Cecal Ligation ◽  
Sham Operation ◽  
Cecal Ligation And Puncture ◽  
Nicotinic Acetylcholine ◽  
Anterior Tibial Muscle ◽  
Anterior Tibial ◽  
Alpha Bungarotoxin ◽  
Peritonitis Model

Background Although systemic inflammation is believed to cause upregulation of nicotinic acetylcholine receptors (nAchRs) in muscle, chronic infections such as Chagas' disease occasionally are complicated by myasthenia gravis. The authors investigated how a nonlethal cecal ligation and puncture (CLP) peritonitis model in rats could affect muscle nAchR. Methods On day 1, 4, 7, 14, or 21 after CLP or sham operation, nAchR binding was assayed in the anterior tibial muscle and diaphragm using [125I]alpha-bungarotoxin. The presence or absence of weakness, in vivo dose-response relationships for d-tubocurarine, and serum anti-nAchR antibody titers were assayed in separate experiments. Results Systemic inflammation was most severe during the first 4 to 5 days. Numbers of nAchRs were decreased in anterior tibial muscle on days 7, 14, and 21 after CLP, and in the diaphragm on days 7 and 14 (P < 0.01). Both 50% and 90% blocking doses of d-tubocurarine) were lower in CLP rats than in sham-operated rats on days 7, 14, and 21 (P < .05). Weakness was overt in approximately half of CLP rats at these times. Serum anti-nAchR antibody (0.7-1.4 nM) was detectable beginning on day 4 and continuing throughout the 21-day observation period in 58-67% of CLP rats. Conclusions During the recovery phase of injury, nonlethal CLP peritonitis resulted in downregulation of nAchR. However, further study is needed to determine the role of anti-nAchR antibodies in the development of decreased receptor numbers and impaired neuromuscular function.

scholarly journals Rat Model of Cecal Ligation and Puncture versus Colon Ascendens Stent Peritonitis: Comparative Study for Oxidative Stress

2017 ◽  
Vol 07 (03) ◽  
pp. 80-92
Author(s):  
Lei Chen ◽  
Daiyin Cao ◽  
Enhe Liu ◽  
Chaoxing Xiao ◽  
Mengran Xiong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Comparative Study ◽  
Rat Model ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture ◽  
Colon Ascendens

Juglone Attenuates Sepsis-Induced Acute Lung Injury via Suppression of the TLR4/Nf-κb Pathway

2020 ◽  
Vol 18 (2) ◽  
pp. 201-206
Author(s):  
Qiu Nan ◽  
Xu Xinmei ◽  
He Yingying ◽  
Fan Chengfen
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Nuclear Factor Kappa B ◽  
Cecal Ligation ◽  
Myeloperoxidase Activity ◽  
Nuclear Factor ◽  
Cecal Ligation And Puncture ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Nuclear Factor Kappa

Sepsis, with high mortality, induces deleterious organ dysfunction and acute lung injury. Natural compounds show protective effect against sepsis-induced acute lung injury. Juglone, a natural naphthoquinone, demonstrates pharmacological actions as a pro-apoptotic substrate in tumor treatment and anti-inflammation substrate in organ injury. In this study, the influence of juglone on sepsis-induced acute lung injury was investigated. First, a septic mice model was established via cecal ligation and puncture, and then verified via histopathological analysis of lung tissues, the wet/dry mass ratio and myeloperoxidase activity was determined. Cecal ligation and puncture could induce acute lung injury in septic mice, as demonstrated by alveolar damage and increase of wet/dry mass ratio and myeloperoxidase activity. However, intragastric administration juglone attenuated cecal ligation and puncture-induced acute lung injury. Secondly, cecal ligation and puncture-induced increase of inflammatory cells in bronchoalveolar lavage fluid was also alleviated by the administration of juglone. Similarly, the protective effect of juglone against cecal ligation and puncture-induced acute lung injury was accompanied by a reduction of pro-inflammatory factor secretion in bronchoalveolar lavage fluid and lung tissues. Cecal ligation and puncture could activate toll-like receptor 4/nuclear factor-kappa B signaling pathway, and administration of juglone suppressed toll-like receptor 4/nuclear factor-kappa B activation. In conclusion, juglone attenuated cecal ligation and puncture-induced lung damage and inflammatory response through inactivation of toll-like receptor 4/nuclear factor-kappa B, suggesting a potential therapeutic strategy in the treatment of sepsis-induced acute lung injury.

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