scholarly journals Characterization of Biological Aerosols Over Northeast India: A Metagenomic Approach

2021 ◽  
Author(s):  
Binita Pathak ◽  
Ankita Khataniar ◽  
Barlin Das ◽  
Sristisri Upadhyaya ◽  
Ankita Medhi ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Monsoon Season ◽  
Fungal Spores ◽  
Northeast India ◽  
Microbial Biodiversity ◽  
Operational Taxonomic Units ◽  
Biological Aerosols ◽  
Four Seasons ◽  
Metagenomic Approach

Abstract Northeast India is considered as one of the major biodiversity hotspots in the world but the region is underexplored for their microbial biodiversity. Extensive characterization of biological aerosol (bioaerosol) samples collected from various locations of Northeast India was carried out for all the four seasons in a year. These were characterized in terms of particulate matters (inhalable, thoracic, and alveolic), their constituents (pollens, fungal spores, animal debris, and non-biological components), and finally the bacterial diversity was determined by DNA based metagenomic approach. The non-biological (non-viable) component of aerosols is found to vary from 30- 89% in pre-monsoon season which coexists with pollens (4-20%), animal debris (1-24%) and fungal spores (1-17%). The highest number of culturable microbial population in terms of CFU count was observed in the samples collected in pre-monsoon season (i.e., 125.24-632.45 CFU/m3) and the lowest CFU was observed in monsoon season (i.e., 20.83- 319.0 CFU/m3). The metagenomic approach with the samples collected during pre-monsoon season showed a total of bacterial 184 OTUs (operational taxonomic units) with 28,028 abundance count comprising with 7 major phylum, 6 classes, 10 orders, 15 families, 13 genus, and 8 species of bacteria. The species level distribution clearly shows the presence of Gammaproteobacteria (52%) most abundantly followed by Bacilli (21%), Alphaproteobacteria (14%), Betaproteobacteria (5%, Flavobacteria (5%), and Sphingobacteria (3%). It is the first report from entire Northeast India to uncover bacterial diversity in aerosol samples through DNA based metagenomic approach.

scholarly journals Characterization of efficient xylanases from industrial-scale pulp and paper wastewater treatment microbiota

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jia Wang ◽  
Jiawei Liang ◽  
Yonghong Li ◽  
Lingmin Tian ◽  
Yongjun Wei
Keyword(s):  
Wastewater Treatment ◽  
Xylanase Activity ◽  
Pulp And Paper ◽  
Optimal Conditions ◽  
Paper Wastewater ◽  
Termite Hindgut ◽  
Uncultured Microorganisms ◽  
Metagenomic Approach ◽  
Gh10 Family

AbstractXylanases are widely used enzymes in the food, textile, and paper industries. Most efficient xylanases have been identified from lignocellulose-degrading microbiota, such as the microbiota of the cow rumen and the termite hindgut. Xylanase genes from efficient pulp and paper wastewater treatment (PPWT) microbiota have been previously recovered by metagenomics, assigning most of the xylanase genes to the GH10 family. In this study, a total of 40 GH10 family xylanase genes derived from a certain PPWT microbiota were cloned and expressed in Escherichia coli BL21 (DE3). Among these xylanase genes, 14 showed xylanase activity on beechwood substrate. Two of these, PW-xyl9 and PW-xyl37, showed high activities, and were purified to evaluate their xylanase properties. Values of optimal pH and temperature for PW-xyl9 were pH 7 and 60 ℃, respectively, while those for PW-xyl37 were pH 7 and 55 ℃, respectively; their specific xylanase activities under optimal conditions were 470.1 U/mg protein and 113.7 U/mg protein, respectively. Furthermore, the Km values of PW-xyl9 and PW-xyl37 were determined as 8.02 and 18.8 g/L, respectively. The characterization of these two xylanases paves the way for potential application in future pulp and paper production and other industries, indicating that PPWT microbiota has been an undiscovered reservoir of efficient lignocellulase genes. This study demonstrates that a metagenomic approach has the potential to screen efficient xylanases of uncultured microorganisms from lignocellulose-degrading microbiota. In a similar way, other efficient lignocellulase genes might be identified from PPWT treatment microbiota in the future.

scholarly journals New Insights on the Zeugodacus cucurbitae (Coquillett) Bacteriome

2021 ◽  
Vol 9 (3) ◽  
pp. 659
Author(s):  
Elias Asimakis ◽  
Panagiota Stathopoulou ◽  
Apostolis Sapounas ◽  
Kanjana Khaeso ◽  
Costas Batargias ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Mutual Exclusion ◽  
Bacterial Composition ◽  
Operational Taxonomic Units ◽  
Host Diet ◽  
Dominant Bacteria ◽  
High Degree

Various factors, including the insect host, diet, and surrounding ecosystem can shape the structure of the bacterial communities of insects. We have employed next generation, high-throughput sequencing of the 16S rRNA to characterize the bacteriome of wild Zeugodacus (Bactrocera) cucurbitae (Coquillett) flies from three regions of Bangladesh. The tested populations developed distinct bacterial communities with differences in bacterial composition, suggesting that geography has an impact on the fly bacteriome. The dominant bacteria belonged to the families Enterobacteriaceae, Dysgomonadaceae and Orbaceae, with the genera Dysgonomonas, Orbus and Citrobacter showing the highest relative abundance across populations. Network analysis indicated variable interactions between operational taxonomic units (OTUs), with cases of mutual exclusion and copresence. Certain bacterial genera with high relative abundance were also characterized by a high degree of interactions. Interestingly, genera with a low relative abundance like Shimwellia, Gilliamella, and Chishuiella were among those that showed abundant interactions, suggesting that they are also important components of the bacterial community. Such knowledge could help us identify ideal wild populations for domestication in the context of the sterile insect technique or similar biotechnological methods. Further characterization of this bacterial diversity with transcriptomic and metabolic approaches, could also reveal their specific role in Z. cucurbitae physiology.

scholarly journals Characterization of oral bacterial diversity of irradiated patients by high-throughput sequencing

2013 ◽  
Vol 5 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Yue-Jian Hu ◽  
Qian Wang ◽  
Yun-Tao Jiang ◽  
Rui Ma ◽  
Wen-Wei Xia ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
High Throughput ◽  
High Throughput Sequencing

scholarly journals Karyo-morphological characterization of natural genetic variation in some threatenedCymbidiumspecies of Northeast India

Caryologia ◽  
2010 ◽  
Vol 63 (1) ◽  
pp. 99-105 ◽  
Author(s):  
Santosh Kumar Sharma ◽  
Khedasana Rajkumari ◽  
Suman Kumaria ◽  
Pramod Tandon ◽  
Satyawada Rama Rao
Keyword(s):  
Genetic Variation ◽  
Northeast India ◽  
Morphological Characterization ◽  
Natural Genetic Variation

scholarly journals Erratum to: Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

2014 ◽  
Vol 52 (12) ◽  
pp. 1056-1056
Author(s):  
Ok-Hwa Hwang ◽  
Sebastian Raveendar ◽  
Young-Ju Kim ◽  
Ji-Hun Kim ◽  
Tae-Hun Kim ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Bacterial Diversity ◽  
16S Rdna ◽  
Rdna Sequence ◽  
Pig Slurry ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

Effects of antibiotics on the development and intestinal bacterial diversity of black soldier fly larvae

2021 ◽  
pp. 1-14
Author(s):  
J.B. Zhang ◽  
Y. Meng ◽  
J. Xu ◽  
C. Rensing ◽  
D. Wang
Keyword(s):  
Bacterial Diversity ◽  
Larval Growth ◽  
Anaerobic Bacteria ◽  
Inhibitory Effect ◽  
High Dose ◽  
Streptomycin Sulphate ◽  
Black Soldier Fly ◽  
Operational Taxonomic Units ◽  
Black Soldier Fly Larvae ◽  
Hermetia Illucens Larvae

The effects of four antibiotics (metronidazole (M) levofloxacin (L), sodium ampicillin (A), and streptomycin sulphate (S)) and their pair-wise combinations at three doses on the development and intestinal bacterial diversity of the black soldier fly (BSF; Hermetia illucens) larvae were studied. At a low dose M and L were able to inhibit larval growth. At a high dose, all antibiotics were shown to inhibit larval growth. However, the pair-wise combinational use of the antibiotics did not effectively enhance the inhibitory effect. The gut bacterial diversity of the normal control (NC) was significantly higher than the antibiotic-treated groups with 737 operational taxonomic units (OTUs) from the larval guts of NC, compared to 305 and 227 from ML and AS. The number of anaerobic bacteria in ML was significantly lower than in NC and AS, with the relative abundance of OTUs from larval guts of ML being only about 0.01, compared to 0.4 for NC and 0.15 for AS. These results indicated that antibiotics at the experimental concentration did not affect the palatability of food for insects, but they would affect the diversity of food and intestinal microorganisms of BSF larvae, and the inhibitory effect of antibiotics on growth and development of BSF larvae displayed in this study was a complex effect.

scholarly journals Microbiology and atmospheric processes: chemical interactions of primary biological aerosols

2008 ◽  
Vol 5 (4) ◽  
pp. 1073-1084 ◽  
Author(s):  
L. Deguillaume ◽  
M. Leriche ◽  
P. Amato ◽  
P. A. Ariya ◽  
A.-M. Delort ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Atmospheric Chemistry ◽  
Ph Value ◽  
Chemical Constituents ◽  
Fungal Spores ◽  
Chemical Properties ◽  
Nutrient Concentrations ◽  
Chemical Interactions ◽  
Biological Aerosols ◽  
Micro Organisms

Abstract. This paper discusses the influence of primary biological aerosols (PBA) on atmospheric chemistry and vice versa through microbiological and chemical properties and processes. Several studies have shown that PBA represent a significant fraction of air particulate matter and hence affect the microstructure and water uptake of aerosol particles. Moreover, airborne micro-organisms, namely fungal spores and bacteria, can transform chemical constituents of the atmosphere by metabolic activity. Recent studies have emphasized the viability of bacteria and metabolic degradation of organic substances in cloud water. On the other hand, the viability and metabolic activity of airborne micro-organisms depend strongly on physical and chemical atmospheric parameters such as temperature, pressure, radiation, pH value and nutrient concentrations. In spite of recent advances, however, our knowledge of the microbiological and chemical interactions of PBA in the atmosphere is rather limited. Further targeted investigations combining laboratory experiments, field measurements, and modelling studies will be required to characterize the chemical feedbacks, microbiological activities at the air/snow/water interface supplied to the atmosphere.

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