Effects of antibiotics on the development and intestinal bacterial diversity of black soldier fly larvae

2021 ◽  
pp. 1-14
Author(s):  
J.B. Zhang ◽  
Y. Meng ◽  
J. Xu ◽  
C. Rensing ◽  
D. Wang
Keyword(s):  
Bacterial Diversity ◽  
Larval Growth ◽  
Anaerobic Bacteria ◽  
Inhibitory Effect ◽  
High Dose ◽  
Streptomycin Sulphate ◽  
Black Soldier Fly ◽  
Operational Taxonomic Units ◽  
Black Soldier Fly Larvae ◽  
Hermetia Illucens Larvae

The effects of four antibiotics (metronidazole (M) levofloxacin (L), sodium ampicillin (A), and streptomycin sulphate (S)) and their pair-wise combinations at three doses on the development and intestinal bacterial diversity of the black soldier fly (BSF; Hermetia illucens) larvae were studied. At a low dose M and L were able to inhibit larval growth. At a high dose, all antibiotics were shown to inhibit larval growth. However, the pair-wise combinational use of the antibiotics did not effectively enhance the inhibitory effect. The gut bacterial diversity of the normal control (NC) was significantly higher than the antibiotic-treated groups with 737 operational taxonomic units (OTUs) from the larval guts of NC, compared to 305 and 227 from ML and AS. The number of anaerobic bacteria in ML was significantly lower than in NC and AS, with the relative abundance of OTUs from larval guts of ML being only about 0.01, compared to 0.4 for NC and 0.15 for AS. These results indicated that antibiotics at the experimental concentration did not affect the palatability of food for insects, but they would affect the diversity of food and intestinal microorganisms of BSF larvae, and the inhibitory effect of antibiotics on growth and development of BSF larvae displayed in this study was a complex effect.

scholarly journals Effects of rearing system and microbial inoculation on black soldier fly larvae growth and microbiota when reared on agri-food by-products

2021 ◽  
pp. 1-16
Author(s):  
M. Gold ◽  
T. Fowles ◽  
J.D. Fernandez-Bayo ◽  
L. Palma Miner ◽  
C. Zurbrügg ◽  
...  
Keyword(s):  
Moisture Content ◽  
System Design ◽  
Larval Growth ◽  
Dry Mass ◽  
Black Soldier Fly ◽  
Performance Improvements ◽  
Substrate Reduction ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae ◽  
By Products

Black soldier fly larvae (BSFL) are widely used in recycling and upcycling of nutrients in agri-food by-products, but low and inconsistent BSFL rearing performance (i.e. larval growth, bioconversion rate, and substrate reduction) has been identified as a key challenge. The aims of this research were two-fold: (1) validate an existing closed rearing system design; and (2) assess whether a microbial inoculum derived from the rearing residue increases rearing performance. In controlled bench-scale experiments, BSFL were reared on tomato pomace (TP) and white wine pomace (WWP), along with food waste as control substrate. The two aims were assessed based on the following response variables: larval mass, substrate reduction, residue properties (i.e. pH, temperature, moisture content), and larval intestinal and residue microbiota. Higher BSFL mass (by 5.1 mg dry mass) at harvest on WWP and substrate reduction on TP (by 11.7% dry mass) in the closed system compared to the open system confirmed the potential of closed systems for rearing performance improvements of agri-food by-products. The rearing system also affected the residual moisture content and temperature, but only had a small effect on microbiota. Performance improvements by the closed rearing system design may be outweighed by insufficient aeration with pasty substrates and higher operational efforts for aeration and larval separation from the high-moisture residues. In contrast to the rearing system design, addition of the residue-derived microbial inoculum did not result in improved performance, nor did it alter intestinal and residue microbiota. Missing performance improvements could have been due to absent or low numbers of probiotic bacteria. The success of microbial substrate supplementation could be improved by studying effects of larval-associated microbes and developing cultivation methods that selectively amplify the beneficial (yet unknown) members of the microbial community. Our investigations aimed to increase the valorisation of low-value agri-food by-products in BSFL rearing.

scholarly journals Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

2021 ◽  
Author(s):  
Yanxian Li ◽  
Karina Gajardo ◽  
Alexander Jaramillo-Torres ◽  
Trond M. Kortner ◽  
Ashild Krogdahl
Keyword(s):  
Atlantic Salmon ◽  
Intestinal Microbiota ◽  
Feeding Trial ◽  
Feed Ingredient ◽  
Black Soldier Fly ◽  
Nutritional Values ◽  
Distal Intestine ◽  
Relevant Reference ◽  
Black Soldier Fly Larvae ◽  
Hermetia Illucens Larvae

Being part of fish's natural diets, insects have become a realistic, sustainable feed ingredient for aquaculture. While nutritional values of insects have been extensively studied in various fish species, their impact on the fish microbiota remains to be fully explored. In an 8-week freshwater feeding trial, Atlantic salmon (Salmo salar) were fed either a commercially relevant reference diet or an insect meal diet wherein black soldier fly (Hermetia illucens) larvae meal comprised 60% of total ingredients. Microbiota of digesta and mucosa origin from the proximal and distal intestine were collected and profiled along with feed and water samples. The insect meal diet markedly modulated the salmon intestinal microbiota . Overall, the microbial diversity was lower in the digesta of salmon fed the insect meal diet but higher in the mucosa. A group of bacterial genera, dominated by members of the Bacillaceae family, was enriched in salmon fed the insect meal diet, which confirms our previous findings in a seawater feeding trial. We also found that microbiota in the intestine closely resembled that of the feeds but was distinct from the water microbiota. Notably, bacterial genera associated with the diet effects were present in the feeds as well. In conclusion, our results show consistent changes in the intestinal microbiota of Atlantic salmon fed diets containing black soldier fly larvae meal.

scholarly journals Growth of Yellowtail (Seriola quinqueradiata) Fed on a Diet Including Partially or Completely Defatted Black Soldier Fly (Hermetia illucens) Larvae Meal

Insects ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 722
Author(s):  
Atsushi Ido ◽  
Muhammad-Fariz-Zahir Ali ◽  
Takayuki Takahashi ◽  
Chiemi Miura ◽  
Takeshi Miura
Keyword(s):  
Protein Composition ◽  
Fish Growth ◽  
Farmed Fish ◽  
Black Soldier Fly ◽  
Promising Alternative ◽  
Seriola Quinqueradiata ◽  
Fat Fraction ◽  
Feeding Trials ◽  
Black Soldier Fly Larvae ◽  
Hermetia Illucens Larvae

Against a background of increased demand for fish meal (FM), black soldier fly larva is a promising alternative feed source for sustainable aquaculture. Yellowtail, the most popular farmed fish in Japan, is a carnivorous fish; therefore, it requires a high proportion of FM in its diet. This study represents the first example of yellowtail fed on a diet including insect meal as a replacement for FM. Partially defatted black soldier fly meal (PDBM) comprised 49.0% crude protein and 23.2% crude fat, while completely defatted black soldier fly meal (CDBM) contained less than 10% crude fat, as the same level as FM was achieved with defatting PDBM using hexane. In feeding trials, growth of the fish was reduced in accordance with PDBM content: 10%, 20%, and 30% in their diet. Although a diet including 8% CDBM (with the same protein composition as 10% PDBM) also resulted in decreased fish growth, growth with a diet including 16% CDBM (with the same protein composition as 20% PDBM) was significantly higher than that of 20% PDBM, and equivalent to that of 10% PDBM. Therefore, even 10% of partially or completely black soldier fly larvae meal in diets inhibited growth in juvenile yellowtail, and we found that removal of the fat fraction could improve fish growth.

Development of black soldier fly larvae (Diptera: Stratiomyidae) on seafood wastes

2019 ◽  
Vol 5 (4) ◽  
pp. 313-319 ◽  
Author(s):  
J. Villazana ◽  
A. Alyokhin
Keyword(s):  
Sea Urchin ◽  
Sea Cucumber ◽  
Larval Mortality ◽  
Larval Growth ◽  
Published Data ◽  
Black Soldier Fly ◽  
Growth And Survival ◽  
Short Period ◽  
Similar Materials ◽  
Black Soldier Fly Larvae

The black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae) is well suited for remediating biological waste. Larvae of this species are nutrient rich, grow rapidly, and convert organic wastes while suppressing pathogens and pests. Therefore, they could be used in waste management systems and as feed for livestock and in aquaculture. The goal of this study was to test suitability of several seafood wastes from Maine processing facilities for H. illucens development in order to find alternative venues for their utilization. The following substrates were tested under laboratory conditions: dry sea cucumber, wet sea cucumber, crab meal, finfish, dry quahog, wet quahog, and sea urchin (‘wet’ and ‘dry’ refer to the original condition of the wastes while their moisture levels were similar during the experiment). H. illucens eggs were placed on wax paper above each substrate, and larval growth and survival to pupation were measured. Based on the numbers and weights of surviving larvae, substrates potentially suitable for H. illucens rearing included finfish trimmings, wet sea cucumber, dry quahog, and sea urchin. Crab meal produced large larvae (0.091±0.021 g [mean ± SE], compared to experiment-wide mean of 0.064±0.007 g) in a relatively short period of time. However, larval mortality was very high, with only 4.7±1.17 larvae surviving to prepupae, compared to experiment-wide mean of 9.78±0.63 larvae. There were dramatic differences in seemingly similar materials. On wet sea cucumber, 28.82±1.49 larvae per replication survived to pre-pupae, while on dry sea cucumber the number was only 0.33±0.01. On wet and dry quahog, the numbers of surviving larvae were 1.83±0.60 and 10.1±1.97, respectively. This highlights the importance of thorough testing of specific substrates instead of extrapolating from published data.

scholarly journals Rhizopus oligosporus-Assisted Valorization of Coconut Endosperm Waste by Black Soldier Fly Larvae for Simultaneous Protein and Lipid to Biodiesel Production

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 299
Author(s):  
Chung Yiin Wong ◽  
Kunlanan Kiatkittipong ◽  
Worapon Kiatkittipong ◽  
Jun Wei Lim ◽  
Man Kee Lam ◽  
...  
Keyword(s):  
Larval Growth ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Ex Situ ◽  
Rhizopus Oligosporus ◽  
Black Soldier Fly ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae ◽  
Complex Organic

Coconut endosperm waste (CEW) was treated by Rhizopus oligosporus via in situ and ex situ fermentations together with bioconversion into valuable black soldier fly larval biomass. The ex situ fermentation could overall enrich the nutritional compositions of CEW by hydrolyzing its complex organic polymers and exuding assimilable nutrients to enhance the black soldier fly larvae (BSFL) growth. Nevertheless, the larval gut bacteria were competing with Rhizopus oligosporus in in situ fermentation, derailing the hydrolysis processes and larval growth. Accordingly, the highest growth rates achieved were around 0.095 g/day, as opposed to only 0.065 g/day whilst using 0.5 wt% of Rhizopus oligosporus to perform ex situ and in situ fermentations, respectively. These were also underpinned by the greater amount of total CEW consumed when employing ex situ fermentation, with comparable metabolic costs to feeding on in situ-fermented CEW. The mature BSFL were subsequently harvested and the amounts of protein and lipid produced were assessed in terms of their feasibility for biodiesel production. While the statistical analyses showed that the larval protein yields derived from both fermentation modes were insignificant, the BSFL could attain higher lipid and protein productivities upon feeding with ex situ- rather than in situ-fermented CEW mediums. Better yet, the larval biodiesel quality measured in terms of the fatty acid methyl ester composition were not varied significantly by Rhizopus oligosporus through the fermentation process. Thereby, the presence of 1.0 wt% Rhizopus oligosporus was considered optimum to perform ex situ fermentation, giving rise to the acceptable growth of BSFL loaded with the highest lipid yield and productivity for producing biodiesel and protein simultaneously.

scholarly journals Unlocking the Real Potential of Black Soldier Fly (Hermetia illucens) Larvae Protein Derivatives in Pet Diets

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4216
Author(s):  
Ange Mouithys-Mickalad ◽  
Nuria Martin Tome ◽  
Thomas Boogaard ◽  
Arpita Chakraborty ◽  
Didier Serteyn ◽  
...  
Keyword(s):  
Antimicrobial Activities ◽  
Ros Production ◽  
Pet Food ◽  
Hydrogen Atoms ◽  
Black Soldier Fly ◽  
Food Ingredients ◽  
Unstable Molecules ◽  
Black Soldier Fly Larvae ◽  
Proteinase Inhibition ◽  
Hermetia Illucens Larvae

Black soldier fly larvae (BSFL)-derived proteins are gaining popularity as sustainable pet food ingredients. According to the literature, these ingredients have strong antioxidant and antimicrobial activities. Due to the ability of BSFL protein derivatives to donate hydrogen atoms and/or electrons to counterpoise unstable molecules, they could possibly help in the prevention of osteoarthritis. During this study, the antiarthritic potential of BSFL protein derivatives was evaluated using the following assays: (1) proteinase inhibition, (2) erythrocyte membrane stability, (3) reactive oxygen species (ROS) production by activated macrophages, (4) ROS production by monocytes, and (5) cellular toxicity. Additionally, the glucosamine content of these ingredients was also evaluated. Chicken meal is commonly used in pet food formulations and was used as an industrial benchmark. The results obtained during this study demonstrated the strong antiarthritic potential of BSFL protein derivatives. We found that BSFL protein derivatives are not only useful in preventing the development of arthritis but could also help to cure it due to the presence of glucosamine. We also found that chicken meal could contribute to the development of arthritis by increasing ROS production by monocytes.

scholarly journals Blended Sewage Sludge–Palm Kernel Expeller to Enhance the Palatability of Black Soldier Fly Larvae for Biodiesel Production

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 297
Author(s):  
Ratchaprapa Raksasat ◽  
Kunlanan Kiatkittipong ◽  
Worapon Kiatkittipong ◽  
Chung Yiin Wong ◽  
Man Kee Lam ◽  
...  
Keyword(s):  
Sewage Sludge ◽  
Organic Waste ◽  
Larval Growth ◽  
Palm Kernel ◽  
Larval Weight ◽  
Black Soldier Fly ◽  
Lipid Yield ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae ◽  
Fame Profile

Black soldier fly larvae (BSFL) have been employed for valorizing organic waste materials as the larvae are able to consume organic waste and transform it into valuable larval biomass. In this study, BSFL were found to potentially reduce blended sewage sludge. The addition of palm kernel expeller (PKE) fortified the protein and lipid content in blended sewage sludge substrates, leading to larval growth enhancement. In addition, the larval weight also influenced the lipid yield and fatty acid methyl ester (FAME) profile. However, the optimum ratio of sewage sludge to PKE had to be determined as excess PKE content could become a threat to larval growth by contributing to the reduction of non-fiber carbohydrates content in the feed, thereby resulting in the decrease in lipid yield and FAME content. In this work, a sewage sludge to PKE ratio of 2:3 proffered the highest larval weight gained at 46.99 ± 2.09 mg/larva. Meanwhile, a proportion of 3:2 of sewage sludge to PKE was able provide the highest lipid yield of 17 ± 1.77%. Furthermore, the FAME profile revealed the presence of a significant amount of saturated and monosaturated fatty acids, indicating a good quality biodiesel. Thus, BSFL-based biodiesel fed with blended sewage sludge and PKE could be utilized for producing a high quality biodiesel. However, further improvement on the amount of lipid yield and FAME content should be further investigated.

scholarly journals Impact of black soldier fly larvae meal on the chemical and nutritional characteristics of rainbow trout fillets

animal ◽  
2018 ◽  
Vol 12 (8) ◽  
pp. 1672-1681 ◽  
Author(s):  
S. Mancini ◽  
I. Medina ◽  
V. Iaconisi ◽  
F. Gai ◽  
A. Basto ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Black Soldier Fly ◽  
Nutritional Characteristics ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

Pseudo-Parkinson Disease Secondary to Ritonavir–Buspirone Interaction

2003 ◽  
Vol 37 (2) ◽  
pp. 202-205 ◽  
Author(s):  
Patrick G Clay ◽  
Molly M Adams
Keyword(s):  
Drug Interaction ◽  
Inhibitory Effect ◽  
Hiv Positive ◽  
High Dose ◽  
Resting Tremor ◽  
Case Summary ◽  
History Of ◽  
Drug Drug Interaction ◽  
To Receive

OBJECTIVE: To report a case of Parkinson-like symptoms appearing in a patient after introduction of ritonavir to buspirone therapy. CASE SUMMARY: A 54-year-old HIV-positive white man presented to the clinic with a 2-week history of ataxia, shuffling gait, cogwheel rigidity, resting tremor, and sad affect with masked features. This patient had been receiving high-dose buspirone (40 mg every morning and 30 mg every evening) for 2 years prior to the introduction of ritonavir/indinavir combination therapy (400 mg/400 mg twice daily) 6 weeks prior to initiation of the above symptoms. Buspirone was decreased to 15 mg 3 times daily, ritonavir/indinavir was discontinued, and amprenavir 1200 mg twice daily was added. The patient's symptoms began to subside after 1 week, with complete resolution after about 2 weeks. The patient continued to receive buspirone for an additional 12 months without recurrence of symptoms. DISCUSSION: This is the first reported interaction of buspirone and antiretrovirals. Buspirone, extensively metabolized by CYP3A4, was likely at supratherapeutic levels due to the inhibitory effect of ritonavir and, secondarily, indinavir. The Parkinson-like symptoms developed rapidly and severely, impacted this patient's quality of life, and necessitated significant clinic expenditures to identify this drug–drug interaction. CONCLUSIONS: This case demonstrates a severe drug–drug interaction between buspirone and ritonavir and further demonstrates the need for awareness of the metabolic profile for all agents an HIV-infected patient is receiving.

scholarly journals Valorisation of Organic Waste By-Products Using Black Soldier Fly (Hermetia illucens) as a Bio-Convertor

2021 ◽  
Vol 13 (15) ◽  
pp. 8345
Author(s):  
Kieran Magee ◽  
Joe Halstead ◽  
Richard Small ◽  
Iain Young
Keyword(s):  
Food Waste ◽  
Fish Feed ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
High Quality Protein ◽  
The Face ◽  
Beet Pulp ◽  
By Products ◽  
Hermetia Illucens Larvae

One third of food produced globally is wasted. Disposal of this waste is costly and is an example of poor resource management in the face of elevated environmental concerns and increasing food demand. Providing this waste as feedstock for black soldier fly (Hermetia illucens) larvae (BSFL) has the potential for bio-conversion and valorisation by production of useful feed materials and fertilisers. We raised BSFL under optimal conditions (28 °C and 70% relative humidity) on seven UK pre-consumer food waste-stream materials: fish trimmings, sugar-beet pulp, bakery waste, fruit and vegetable waste, cheese waste, fish feed waste and brewer’s grains and yeast. The nutritional quality of the resulting BSFL meals and frass fertiliser were then analysed. In all cases, the volume of waste was reduced (37–79%) and meals containing high quality protein and lipid sources (44.1 ± 4.57% and 35.4 ± 4.12%, respectively) and frass with an NPK of 4.9-2.6-1.7 were produced. This shows the potential value of BSFL as a bio-convertor for the effective management of food waste.

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