scholarly journals Evaluation of Bacterial Diversity in a Swine Manure Composting System Contaminated With Veterinary Antibiotics (VAs)

2022 ◽  
Author(s):  
Robson Evaldo Gehlen Bohrer ◽  
Elvis Carissimi ◽  
Divanilde Guerra ◽  
Danni Maisa Silva ◽  
Eduardo de Souza Lorensi ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Bacterial Community ◽  
Bacterial Diversity ◽  
Swine Manure ◽  
Veterinary Drugs ◽  
Veterinary Antibiotics ◽  
Composting Process ◽  
Wood Shavings ◽  
Eucalyptus Wood ◽  
Generation Sequencing

Abstract The objective of this work was identify microorganisms present in swine effluent composting system, under the contamination by most use veterinary drugs in Brazil. The composting took place for 150 days, where was addition of 200 liters of manure (these 25 liters initially contaminated with 17 antibiotics) in 25 kg of eucalyptus wood shavings. Microorganisms were measured at times (0 until 150 days), and were identified in the V3-V4 regions of the 16S RNAr for Bacteria, by means of next-generation sequencing (NSG). The results showing 7 different Bacteria Phyla and, 70 Bacteria Genus identified (more than 1% significance), in total there were more than 26 phyla and 585 genera of bacteria. The genus Brucella was found during mesophilic and thermophilic phases, this genus, not yet been reported in article involving composting process. These results suggested the potential of adaptation of the bacterial community with antibiotics denoted through the antibiotics.

scholarly journals Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

2014 ◽  
Vol 97 (2) ◽  
pp. 362-366 ◽  
Author(s):  
Aaron M. Dickey ◽  
Andrew J. Trease ◽  
Antonella Jara-Cavieres ◽  
Vivek Kumar ◽  
Matthew K. Christenson ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Bacterial Diversity ◽  
Next Generation ◽  
Generation Sequencing

Next-Generation Sequencing Analyses of Bacterial Community Structures in Soybean Pastes Produced in Northeast China

2017 ◽  
Vol 82 (4) ◽  
pp. 960-968 ◽  
Author(s):  
Mi-Hwa Lee ◽  
Fan-Zhu Li ◽  
Jiyeon Lee ◽  
Jisu Kang ◽  
Seong-Il Lim ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Bacterial Community ◽  
Northeast China ◽  
Next Generation ◽  
Community Structures ◽  
Generation Sequencing

scholarly journals Bacterial abundance and community composition in green, brown and red water from intensive catfish (Clarias sp.) culture ponds in Yogyakarta, Indonesia

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Karunia Adetera Nungki Wijayanti ◽  
Indah Istiqomah ◽  
Murwantoko Murwantoko
Keyword(s):  
Next Generation Sequencing ◽  
Bacterial Community ◽  
16S Rrna ◽  
Community Composition ◽  
Water Samples ◽  
Bacterial Abundance ◽  
Rrna Gene ◽  
Next Generation ◽  
Aquaculture System ◽  
Generation Sequencing

Abstract. Wijayanti KAN, Istiqomah I, Murwantoko. 2021. Bacterial abundance and community composition in green, brown and red water from intensive Catfish (Clarias sp.) culture ponds in Yogyakarta, Indonesia. Biodiversitas 22: 3677-3684. Catfish (Clarias sp.) is an important aquaculture commodity in Indonesia and cultured in an intensive system. Microorganisms play an important role in maintaining water quality of aquaculture system. The objective of this study was to determine the bacterial abundance and community composition of green, brown and red water collected from intensive catfish culture ponds in Yogyakarta using next-generation sequencing method. The water samples were collected from intensive catfish culture ponds with different colors, namely green, brown and red ponds located in Yogyakarta. The DNA from water samples was extracted using DNA extraction kit and used as template for 16S rRNA amplification. The V3-V4 hypervariable regions of the 16S rRNA gene were amplified apply for next-generation sequencing technology. This study could explore effectively the bacterial community in water samples. The bacterial communities in this catfish culture water showed higher bacterial richness compared to the other aquaculture system. The diversity of the green, brown and red catfish culture water ponds was similar with the number OTUs of the green, brown and red water samples, which were 1269; 1387 and 1323 OTUs respectively. The 694 OTUs (34.42%) were common core microbiomes in all catfish culture ponds, the 212 OTUs (10.51%) are present on green and brown water ponds, the 182 OTUs (9.02%) were on green and red water ponds, and the 183 OTUs (9.07%) were present on green and brown water ponds. However, the composition of the bacterial community was different. The most dominant phylum in green and brown water ponds was Proteobacteria with relative abundance in green water and brown water 71.6% and 47.0% respectively, whereas, the most dominant phylum in red water was Firmicutes (29.5%). The dominance of Firmicutes phylum in red water ponds may be caused by application of probiotic bacteria, the high organic content, and low oxygen concentration.

Inferences of gut bacterial diversity from next-generation sequencing of 16S rDNA in deep sea blind ray - Benthobatis moresbyi

2018 ◽  
Vol 9 ◽  
pp. 1-6 ◽  
Author(s):  
Tina Kollannoor Johny ◽  
Bindiya Ellathuparambil Saidumohamed ◽  
Raghul Subin Sasidharan ◽  
Sarita Ganapathy Bhat
Keyword(s):  
Next Generation Sequencing ◽  
Bacterial Diversity ◽  
16S Rdna ◽  
Deep Sea ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals The equine gastrointestinal microbiome: Impacts of weight-loss

2020 ◽  
Author(s):  
Philippa Morrison ◽  
Charles J. Newbold ◽  
Eleanor Jones ◽  
Hilary J. Worgan ◽  
Dai H. Grove-White ◽  
...  
Keyword(s):  
Weight Loss ◽  
Next Generation Sequencing ◽  
16S Rrna ◽  
Bacterial Diversity ◽  
Body Mass ◽  
Dietary Restriction ◽  
Acetate Concentration ◽  
Negatively Associated ◽  
Faecal Microbiome ◽  
Generation Sequencing

Abstract Background Obesity is an important equine welfare issue. Whilst dietary restriction is the most effective weight-loss tool, individual animals range in their weight-loss propensity. Gastrointestinal-derived bacteria play a fundamental role in host-health and have been associated with obesity and weight-loss in other species. This study evaluated the faecal microbiome (next-generation sequencing of 16S rRNA genes) of 15 obese Welsh Mountain pony mares, in the same 11-week period across 2 years (n = 8 Year 1; n = 7 Year 2). Following a 4-week acclimation period (pre-diet phase) during which time individuals were fed the same hay to maintenance (2% body mass (BM) as daily dry matter (DM) intake), animals underwent a 7-week period of dietary restriction (1% BM hay as daily DM intake). Faeces were sampled on the final 3 days of the pre-diet phase and the final 3 days of the dietary restriction phase. Bacterial communities were determined using Next Generation Sequencing of amplified V1-V2 hypervariable regions of bacterial 16S rRNA. Results Losses in body mass ranged from 7.11% to 11.59%. Changes in the faecal microbiome composition following weight-loss included a reduction in the relative abundance of Firmicutes and Tenericutes and a reduction in indices of bacterial diversity. Pre-diet diversity was negatively associated with weight-loss. Pre-diet faecal acetate concentration was a strong predictor of subsequent weight-loss and negatively associated with Sphaerochaeta (Spirochaetes phylum) abundance. When animals were divided into 3 groups (high, mid, low) based overall weight loss, pre-diet bacterial community structure was found to have the greatest divergence between the high and low weight-loss groups (R = 0.67, p < 0.01), following PERMANOVA and ANOSIM analysis. Conclusions Weight-loss in this group of ponies was associated with lower pre-diet faecal bacterial diversity and greater pre-diet acetate concentration. Overall, these data support a role for the faecal microbiome in weight-loss propensity in ponies and provide a baseline for research evaluating elements of the faecal microbiome in predicting weight-loss success in larger cohorts.

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