scholarly journals Indicators of the molecular pathogenesis of virulent Newcastle Disease Virus in chickens revealed by transcriptomic profiling of spleen

2020 ◽  
Author(s):  
Mohammad Rabiei ◽  
Wai Yee Low ◽  
Milton McAllister ◽  
Yan Ren ◽  
Mohamad Cahyono ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Molecular Pathogenesis ◽  
Bursa Of Fabricius ◽  
Control Group ◽  
Rna Seq ◽  
Virulent Newcastle Disease Virus ◽  
Rho Family Gtpases ◽  
Lymphoid Depletion

Abstract Newcastle disease virus (NDV) has caused significant outbreaks in South-East Asia, particularly in Indonesia in recent years. Recently emerged genotype VII NDVs (NDV-GVII) have shifted their tropism from gastrointestinal/respiratory tropism to a lymphotropic virus, invading lymphoid organs including spleen and bursa of Fabricius to cause profound lymphoid depletion. In this study, we aimed to identify candidate genes and biological pathways that contribute to the disease caused by this neurotropic velogenic NDV-GVII. A transcriptomic analysis based on RNA-Seq of spleen was performed in chickens challenged with NDV-GVII and a control group. In total, 6361 genes were differentially expressed that included 3506 up-regulated genes and 2855 down-regulated genes. Real-Time PCR of ten selected genes validated the RNA-Seq results as the correlation between them is 0.98. Functional and network analysis of DEGs showed altered regulation of ElF2 signalling, mTOR signalling, proliferation of lymphatic system cells, signalling by Rho family GTPases and synaptogenesis signalling in spleen. We have also identified modified expression of IFIT5, PI3K, AGT and PLP1 genes in NDV-GVII infected chickens. Our findings in activation of autophagy-mediated cell death, lymphotropic and synaptogenesis signalling pathways provide new insights into the molecular pathogenesis of this newly emerged NDV-GVII.

scholarly journals Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammad Rabiei ◽  
Wai Yee Low ◽  
Yan Ren ◽  
Mohamad Indro Cahyono ◽  
Phuong Thi Kim Doan ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Molecular Pathogenesis ◽  
Differentially Expressed ◽  
Bursa Of Fabricius ◽  
Control Group ◽  
Rna Seq ◽  
Virulent Newcastle Disease Virus ◽  
Lymphoid Depletion

AbstractNewcastle disease virus (NDV) has caused significant outbreaks in South-East Asia, particularly in Indonesia in recent years. Recently emerged genotype VII NDVs (NDV-GVII) have shifted their tropism from gastrointestinal/respiratory tropism to a lymphotropic virus, invading lymphoid organs including spleen and bursa of Fabricius to cause profound lymphoid depletion. In this study, we aimed to identify candidate genes and biological pathways that contribute to the disease caused by this velogenic NDV-GVII. A transcriptomic analysis based on RNA-Seq of spleen was performed in chickens challenged with NDV-GVII and a control group. In total, 6361 genes were differentially expressed that included 3506 up-regulated genes and 2855 down-regulated genes. Real-Time PCR of ten selected genes validated the RNA-Seq results as the correlation between them is 0.98. Functional and network analysis of Differentially Expressed Genes (DEGs) showed altered regulation of ElF2 signalling, mTOR signalling, proliferation of cells of the lymphoid system, signalling by Rho family GTPases and synaptogenesis signalling in spleen. We have also identified modified expression of IFIT5, PI3K, AGT and PLP1 genes in NDV-GVII infected chickens. Our findings in activation of autophagy-mediated cell death, lymphotropic and synaptogenesis signalling pathways provide new insights into the molecular pathogenesis of this newly emerged NDV-GVII.

Insights into the chicken bursa of fabricius response to Newcastle disease virus at 48 and 72 hours post-infection through RNA-seq

2019 ◽  
Vol 236 ◽  
pp. 108389 ◽  
Author(s):  
Xiangwei Wang ◽  
Yanqing Jia ◽  
Juan Ren ◽  
Haijin Liu ◽  
FathalrhmanEisa Addoma Adam ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Post Infection ◽  
Bursa Of Fabricius ◽  
Rna Seq

scholarly journals DETERMINATION OF IN-VIVO GROWTH KINETICS OF VIRULENT NEWCASTLE DISEASE VIRUS IN BROILER CHICKENS

1970 ◽  
Vol 7 (2) ◽  
pp. 304-312
Author(s):  
MJ Ara ◽  
MT Islam ◽  
MT Hossain ◽  
MA Haque ◽  
R Ahmed ◽  
...  
Keyword(s):  
Cell Culture ◽  
Body Temperature ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Research Work ◽  
Clinical Signs ◽  
Bursa Of Fabricius ◽  
Avian Embryo ◽  
Virulent Newcastle Disease Virus

The research work was conducted on 105 broiler chicks (Cobb-500) with a view to determine the rate of distribution of Newcastle disease virus (NDV) in various organs following infection through natural (intranasal, intraocular and oral) and parenteral (intravenous, intramuscular and subcutaneous) routes of inoculation at different ages (7, 15 and 28 days of old). Each bird received a dose of 0.2 ml of NVNDV (300 ELD50). Body temperature, onset of clinical signs and mortality of birds (if any) were recorded daily. Blood samples were collected from the birds to determine the serum HI titre before and after infection. Faeces and various tissue samples (brain, lungs, kidney, colon, bursa of Fabricius, spleen and thymus) were collected daily following post-mortem examination of one bird from each sub-group to determine the presence of NDV along with their HA titre through inoculation into embryonated hen eggs. Some representative samples were also inoculated into chicken embryo fibroblast (CEF) cell culture for isolation of NDV. The highest body temperature (³1080F) was recorded in the birds of almost all the experimental groups between 48 and 72 hours of PI. Appearance of clinical sings was observed earlier (between 48 to 72 hours of PI) in parenterally infected birds than those of inoculated through natural routes. The shortest duration (>26-54 hours of PI) and longest duration (67-132 hours of PI) of death time recorded in birds those were inoculated through IV and oral routes of infection respectively. Isolation of NDV was positive from day 2 of PI and onward in all the groups with some minor variations in some cases. The CEF cell culture system was found more sensitive compared to avian embryo. Irrespective of routes of inoculation and age of birds, there was significant (p<0.01) increase in the mean HA titre of NDV with the progression of time. The highest HA titre of NDV was found in the brain tissue followed by lungs and kidney. Significantly (p<0.01) higher HA titre of NDV isolate was recorded in the birds of all the experimental groups inoculated through IV route. Following infection, the MDA titres decreased day by day in the birds with the increase of HA titres of NDV.

scholarly journals Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 539
Author(s):  
Congriev Kumar Kabiraj ◽  
Tanjin Tamanna Mumu ◽  
Emdadul Haque Chowdhury ◽  
Mohammad Rafiqul Islam ◽  
Mohammed Nooruzzaman
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Bursa Of Fabricius ◽  
Lymphoid Tissues ◽  
Hemorrhagic Pneumonia ◽  
Advanced Stages ◽  
Lymphoid Depletion ◽  
The Brain ◽  
Bangladeshi Strain

The sequential pathology of a genotype XIII Bangladeshi strain of Newcastle disease virus (NDV) was studied in 5-weeks old chickens. Layer chickens of ISA Brown breed were inoculated through the intranasal and intraocular routes with the BD-C161/2010 strain of NDV and examined at different times post-infection (pi). NDV-infected chickens showed depression at 3 days pi (dpi) followed by dropped wings, paralysis and death starting at 4 dpi. Lungs of infected chickens showed hemorrhagic lesions starting at 24 hours pi (hpi) that was followed by pallor and slight contraction by 2 to 3 dpi and subsequently developed into severe hemorrhagic pneumonia with mononuclear cell infiltration. Hemorrhagic and necrotizing lesions were found in different visceral organs including proventriculus, intestine, gut-associated lymphoid tissues, liver and kidneys starting at 3 dpi that progressed rapidly. Severe lymphoid depletion was observed in the thymus, spleen and bursa of Fabricius starting at 1–3 dpi followed by hemorrhages, necrosis, inflammation and atrophy at 4–5 dpi. In the brain, mild neuronal lesions such as focal to diffuse encephalitis with encephalomalacia was observed at 2–3 dpi and moderate and diffuse meningoencephalitis with encephalomalacia at advanced stages. In conclusion, the BD-C161/2010 strain of NDV produced lesions typical of velogenic viscerotropic pathotype of NDV.

scholarly journals Thymic transcriptome analysis after Newcastle disease virus inoculation in chickens and the influence of host small RNAs on NDV replication

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Liangxing Guo ◽  
Zhaokun Mu ◽  
Furong Nie ◽  
Xuanniu Chang ◽  
Haitao Duan ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Viral Disease ◽  
Immune Genes ◽  
Comprehensive Information ◽  
Virulent Newcastle Disease Virus ◽  
Chicken Thymus ◽  
Innate Immune Genes

AbstractNewcastle disease (ND), caused by virulent Newcastle disease virus (NDV), is a contagious viral disease affecting various birds and poultry worldwide. In this project, differentially expressed (DE) circRNAs, miRNAs and mRNAs were identified by high-throughput RNA sequencing (RNA-Seq) in chicken thymus at 24, 48, 72 or 96 h post LaSota NDV vaccine injection versus pre-inoculation group. The vital terms or pathways enriched by vaccine-influenced genes were tested through KEGG and GO analysis. DE genes implicated in innate immunity were preliminarily screened out through GO, InnateDB and Reactome Pathway databases. The interaction networks of DE innate immune genes were established by STRING website. Considering the high expression of gga-miR-6631-5p across all the four time points, DE circRNAs or mRNAs with the possibility to bind to gga-miR-6631-5p were screened out. Among DE genes that had the probability to interact with gga-miR-6631-5p, 7 genes were found to be related to innate immunity. Furthermore, gga-miR-6631-5p promoted LaSota NDV replication by targeting insulin induced gene 1 (INSIG1) in DF-1 chicken fibroblast cells. Taken together, our data provided the comprehensive information about molecular responses to NDV LaSota vaccine in Chinese Partridge Shank Chickens and elucidated the vital roles of gga-miR-6631-5p/INSIG1 axis in LaSota NDV replication.

scholarly journals High Genetic Diversity of Newcastle Disease Virus in Wild and Domestic Birds in Northeastern China from 2013 to 2015 Reveals Potential Epidemic Trends

2015 ◽  
Vol 82 (5) ◽  
pp. 1530-1536 ◽  
Author(s):  
Pingze Zhang ◽  
Guangyao Xie ◽  
Xinxin Liu ◽  
Lili Ai ◽  
Yanyu Chen ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Phylogenetic Analyses ◽  
Class Ii ◽  
Northeastern China ◽  
Wild Birds ◽  
Class I ◽  
Virulent Newcastle Disease Virus ◽  
Domestic Birds

ABSTRACTNewcastle disease (ND), caused by the virulent Newcastle disease virus (NDV), is one of the most important viral diseases of birds globally, but little is currently known regarding enzootic trends of NDV in northeastern China, especially for class I viruses. Thus, we performed a surveillance study for NDV in northeastern China from 2013 to 2015. A total 755 samples from wild and domestic birds in wetlands and live bird markets (LBMs) were collected, and 10 isolates of NDV were identified. Genetic and phylogenetic analyses showed that five isolates from LBMs belong to class I subgenotype 1b, two (one from wild birds and one from LBMs) belong to the vaccine-like class II genotype II, and three (all from wild birds) belong to class II subgenotype Ib. Interestingly, the five class I isolates had epidemiological connections with viruses from southern, eastern, and southeastern China. Our findings, together with recent prevalence trends of class I and virulent class II NDV in China, suggest possible virus transmission between wild and domestic birds and the potential for an NDV epidemic in the future.

scholarly journals High-level expression of a foreign gene from the most 3′-proximal locus of a recombinant Newcastle disease virus

2001 ◽  
Vol 82 (7) ◽  
pp. 1729-1736 ◽  
Author(s):  
Zhuhui Huang ◽  
Sateesh Krishnamurthy ◽  
Aruna Panda ◽  
Siba K. Samal
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vaccine Vector ◽  
Foreign Gene ◽  
Gene Encoding ◽  
Reading Frame ◽  
Virulent Newcastle Disease Virus ◽  
Nucleocapsid Protein Gene ◽  
High Level

A previous report showed that insertion of a foreign gene encoding chloramphenicol acetyltransferase (CAT) between the HN and L genes of the full-length cDNA of a virulent Newcastle disease virus (NDV) yielded virus with growth retardation and attenuation. The NDV vector used in that study was pathogenic to chickens; it is therefore not suitable for use as a vaccine vector. In the present study, an avirulent NDV vector was generated and its potential to express CAT protein was evaluated. The CAT gene was under the control of NDV transcriptional start and stop signals and was inserted immediately before the open reading frame of the viral 3′-proximal nucleocapsid protein gene. A recombinant NDV expressing CAT activity at a high level was recovered. The replication and pathogenesis of the CAT-expressing recombinant NDV were not modified significantly. These results indicate the potential utility of an avirulent NDV as a vaccine vector.

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