Comparative Performance of Medium-Chain-Length Polyhydroxyalkanoates Production Between Pseudomonas Aeruginosa and Pseudomonas Putida

Abstract Non-degradable polymer waste enlarged over increasing of human population. This badly affected on environmental pollution and biosphere changing. Polyhydroxyalkanoates (PHAs), an eco-friendly biopolymer, were substituted for conventional- or petrochemical-derived polymer. They are reserve carbon energy produced from diversity of microorganisms in granular under limited nutrient for bacterial growth (e.g. nitrogen and phosphorus). Pseudomonas aeruginosa TISTR 1287 was used to catalyze emulsified palm oil to produce valuable PHAs. The highest yield of PHAs production (0.65 g/L, 38.0%) was obtained in MSM supplemented with 0.75% (v/w) emulsified palm oil after 72-hr cultivation, which was a few lower than that produced by Pseudomonas putida TISTR 1522 (0.95 g/L, 40.15%) cultivated in 1% (w/v) fatty acid salt for after 24-hr cultivation. The intracellular PHAs were detected by staining with Nile red . The characters of intracellular PHAs examined by Transmission Electron Microscope (TEM) exhibited that PHAs accumulate in white and roundish-shaped granules with 0.2-0.5 m m diameter inside the cells, about 2-3 granules per rod-shaped bacterium cell. These optimizations were successfully demonstrated high content of intracellular PHAs accumulation in P. aeruginosa TISTR 1287 by utilization of emulsified palm oil.

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Biodegradation of chlorophenols by immobilized pure-culture microorganisms

Water Science & Technology ◽

10.2166/wst.1996.0240 ◽

1996 ◽

Vol 34 (10) ◽

pp. 67-72 ◽

Cited By ~ 5

Author(s):

Lu Chih-Jen ◽

Lee Chi-Mei ◽

Huang Chiou-Zong

Keyword(s):

Pseudomonas Aeruginosa ◽

Pseudomonas Putida ◽

Pure Culture ◽

The Other ◽

Batch Reactors ◽

Agrobacterium Radiobacter ◽

Culture Cells ◽

Lag Period

The biodegradation of phenol and chlorophenols by immobilized pure-culture cells was conducted by a series of batch reactors. The microorganisms used in this study were Pseudomonas putida, Psuedomonas testosteroni, Pseudomonas aeruginosa, and Agrobacterium radiobacter. All four species showed the ortho-cleavage pathway to metabolize chlorophenols. Among the four species, P. testosteroni, P. putida, and P. aeruginosa could effectively remove phenol at 200 mg/l. P. testosteroni could effectively remove 2-chlorophenol at 10mg/l. However, the other three species, P. putida, P. aeruginosa, and A. radiobacter, could not effectively remove 2-chlorophenol. Although 3-chlorophenol is a recalcitrant compound, P. testosteroni also could rapidly metabolize 3-chlorophenol at 10 mg/l. The removal of 4-chlorophenol at 10 mg/l by P. testosteroni reached 98% within one day. P. aeruginosa and A. radiobacter also could metabolize 4-chlorophenol after 2 and 7 days of lag period, respectively.

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High‐throughput dilution‐based growth method enables time‐resolved exo‐metabolomics of Pseudomonas putida and Pseudomonas aeruginosa

Microbial Biotechnology ◽

10.1111/1751-7915.13905 ◽

2021 ◽

Author(s):

Bjarke H. Pedersen ◽

Nicolás Gurdo ◽

Helle Krogh Johansen ◽

Søren Molin ◽

Pablo I. Nikel ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Pseudomonas Putida ◽

High Throughput ◽

Time Resolved ◽

Growth Method

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Imaging and Analysis of Pseudomonas aeruginosa Swarming and Rhamnolipid Production

Applied and Environmental Microbiology ◽

10.1128/aem.06644-11 ◽

2011 ◽

Vol 77 (23) ◽

pp. 8310-8317 ◽

Cited By ~ 27

Author(s):

Joshua D. Morris ◽

Jessica L. Hewitt ◽

Lawrence G. Wolfe ◽

Nachiket G. Kamatkar ◽

Sarah M. Chapman ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Fluorescent Protein ◽

Nile Red ◽

Temporal Distribution ◽

Time Lapse ◽

Content Type ◽

Rhamnolipid Production ◽

Serovar Typhimurium ◽

Surface Motility ◽

Green Fluorescent

ABSTRACTMany bacteria spread over surfaces by “swarming” in groups. A problem for scientists who study swarming is the acquisition of statistically significant data that distinguish two observations or detail the temporal patterns and two-dimensional heterogeneities that occur. It is currently difficult to quantify differences between observed swarm phenotypes. Here, we present a method for acquisition of temporal surface motility data using time-lapse fluorescence and bioluminescence imaging. We specifically demonstrate three applications of our technique with the bacteriumPseudomonas aeruginosa. First, we quantify the temporal distribution ofP. aeruginosacells tagged with green fluorescent protein (GFP) and the surfactant rhamnolipid stained with the lipid dye Nile red. Second, we distinguish swarming ofP. aeruginosaandSalmonella entericaserovar Typhimurium in a coswarming experiment. Lastly, we quantify differences in swarming and rhamnolipid production of severalP. aeruginosastrains. While the best swarming strains produced the most rhamnolipid on surfaces, planktonic culture rhamnolipid production did not correlate with surface growth rhamnolipid production.

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Kinetics of medium-chain-length polyhydroxyalkanoate production by a novel isolate of Pseudomonas putida LS46

Canadian Journal of Microbiology ◽

10.1139/w2012-074 ◽

2012 ◽

Vol 58 (8) ◽

pp. 982-989 ◽

Cited By ~ 24

Author(s):

Parveen K. Sharma ◽

Jilagamazhi Fu ◽

Nazim Cicek ◽

Richard Sparling ◽

David B. Levin

Keyword(s):

Pseudomonas Putida ◽

Chain Length ◽

Saturated Fatty Acids ◽

Dry Mass ◽

Nucleotide Sequence Analysis ◽

Glucose Medium ◽

Medium Chain ◽

Medium Chain Length ◽

Pha Production ◽

Nitrogen Excess

Six bacteria that synthesize medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were isolated from sewage sludge and hog barn wash and identified as strains of Pseudomonas and Comamonas by 16S rDNA gene sequencing. One isolate, Pseudomonas putida LS46, showed good PHA production (22% of cell dry mass) in glucose medium, and it was selected for further studies. While it is closely related to other P. putida strains (F1, KT2440, BIRD-1, GB-1, S16, and W619), P. putida LS46 was genetically distinct from these other strains on the basis of nucleotide sequence analysis of the cpn60 gene hypervariable region. PHA production was detected as early as 12 h in both nitrogen-limited and nitrogen-excess conditions. The increase in PHA production after 48 h was higher in nitrogen-limited cultures than in nitrogen-excess cultures. Pseudomonas putida LS46 produced mcl-PHAs when cultured with glucose, glycerol, or C6–C14 saturated fatty acids as carbon sources, and mcl-PHAs accounted for 56% of the cell dry mass when cells were batch cultured in medium containing 20 mmol/L octanoate. Although 3-hydroxydecanoate was the major mcl-PHA monomer (58.1–68.8 mol%) in P. putida LS46 cultured in glucose medium, 3-hydroxyoctanoate was the major monomer produced in octanoate medium (88 mol%).

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Draft Genome Sequence of Medium-Chain-Length Polyhydroxyalkanoate-Producing Pseudomonas putida Strain LS46

Genome Announcements ◽

10.1128/genomea.00151-13 ◽

2013 ◽

Vol 1 (2) ◽

Cited By ~ 6

Author(s):

P. K. Sharma ◽

J. Fu ◽

X. Zhang ◽

B. W. Fristensky ◽

K. Davenport ◽

...

Keyword(s):

Pseudomonas Putida ◽

Chain Length ◽

Genome Sequence ◽

Draft Genome ◽

Draft Genome Sequence ◽

Medium Chain ◽

Medium Chain Length

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Quantitative ‘Omics Analyses of Medium Chain Length Polyhydroxyalkanaote Metabolism in Pseudomonas putida LS46 Cultured with Waste Glycerol and Waste Fatty Acids

PLoS ONE ◽

10.1371/journal.pone.0142322 ◽

2015 ◽

Vol 10 (11) ◽

pp. e0142322 ◽

Cited By ~ 15

Author(s):

Jilagamazhi Fu ◽

Parveen Sharma ◽

Vic Spicer ◽

Oleg V. Krokhin ◽

Xiangli Zhang ◽

...

Keyword(s):

Fatty Acids ◽

Pseudomonas Putida ◽

Chain Length ◽

Medium Chain ◽

Waste Glycerol ◽

Medium Chain Length

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Conjugal TOL Transfer from Pseudomonas putida to Pseudomonas aeruginosa: Effects of Restriction Proficiency, Toxicant Exposure, Cell Density Ratios, and Conjugation Detection Method on Observed Transfer Efficiencies

Applied and Environmental Microbiology ◽

10.1128/aem.71.1.51-57.2005 ◽

2005 ◽

Vol 71 (1) ◽

pp. 51-57 ◽

Cited By ~ 34

Author(s):

Catalina Arango Pinedo ◽

Barth F. Smets

Keyword(s):

Pseudomonas Aeruginosa ◽

Pseudomonas Putida ◽

Strong Dependence ◽

Plasmid Transfer ◽

Mass Action ◽

Tol Plasmid ◽

Toxicant Exposure ◽

Transfer Frequency ◽

Cell Densities

ABSTRACT The effects of restriction proficiency and premating exposure to toxicants on conjugal transfer of the TOL plasmid between Pseudomonas spp. was investigated by examinations of filter matings. A Pseudomonas putida KT2442-derived strain carrying a gfp-tagged variant of the TOL plasmid was used as a donor, and both restriction-deficient (PAO1162N) and -proficient (PAO2002N) Pseudomonas aeruginosa strains were used as recipients. The in situ enumeration of conjugation events allowed us to obtain frequency estimates that were unbiased by transconjugant growth or plasmid retransfer. We observed a strong dependence of the plasmid transfer frequency on the initial donor-to-recipient ratio of surface matings, which invalidated the use of mass action-based plasmid transfer kinetic estimators. Careful control of the initial parental cell densities permitted evaluations of the true effects of restriction proficiency and toxicant exposure on TOL transfer. At standard donor-to-recipient ratios (10−3 for PAO1162N and 2 Ã¯Â¿Â½ 101 for PAO2002N) and total cell densities (105 cells/mm2 for PAO1162N and 106 cells/mm2 for PAO2002N), plasmid transfer frequencies without toxicant exposure were approximately 10−7 (events/mm2)−1 for PAO1162N and 10−11 (events/mm2)−1 for PAO2002N based on in situ observations of conjugation events. The enumeration of transconjugants via selective plating yielded transfer frequencies that were up to 1 order of magnitude lower. Premating exposure to sodium dodecyl sulfate (1 to 10 mM) significantly increased the transfer frequency for the restriction-proficient strain PAO2002N (P < 0.05) but not for the restriction-deficient strain PAO1162N. On the other hand, premating exposure to ethanol, toluene, or phenol had no positive effect on the plasmid transfer frequency. Clearly, restriction proficiency provides a strong barrier to interspecific transfer of the TOL plasmid, and this barrier was only marginally attenuated by recipient exposure to toxicants within the ranges examined.

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