Neutral Polysaccharide from Panax notoginseng enhanced cyclophosphamide antitumor efficacy in hepatoma H22‑bearing mice

Abstract Background Our previous studies demonstrated that the administration of crude Polysaccharide from Panax notoginseng (CPPN) can effectively prolong the lifespan of tumor-bearing mice via boosting the host immune system as well as weak cytotoxicity against hepatocellular carcinoma (HCC). In the present study, Neutral Polysaccharide (NPPN) were further purified from crude polysaccharide isolated from panax notoginseng. The effects of NPPN on the immune function and hematopoietic function of mice with low immunity and myelosuppression induced by cyclophosphamide (CTX) were investigated. The effect of NPPN combined with CTX on the tumor inhibition rate of the H22 tumor-bearing mice and the impact of NPPN on the proliferation of H22 liver cancer cells in vitro were investigated. Methods CPPN was obtained by water extraction and alcohol precipitation method, and further purified by DEAE Sepharose Fast Flow ion exchange resin column. NPPN was added to the immunosuppressed with myelosuppression mice induced by CTX. Thymus index, spleen index, lymphocyte proliferation stimulation index by adding of concanavalin A, determination of serum hemolysin, NK cell activity assay, mice carbon clearance experiment, blood count tests were detected. The tumor inhibition rate of the H22 tumor-bearing mice treated with NPPN combined with CTX was recorded. Results NPPN and 4 kinds of acid polysaccharide from Panax notoginseng (APPN) were successfully isolated from the CPPN by DEAE Sepharose Fast Flow ion exchange resin column. NPPN inhibited the growth of H22 cells and significantly increase the tumor inhibition rate of the H22 tumor-bearing mice combined with CTX. The elevation of the cellular and humoral immunity levels as well as a variety of blood count tests indicators of immunosuppressive with myelosuppression mice may contribute to the antitumor activity of NPPN. Conclusion NPPN has a potential antitumor activity for the treatment of liver cancer combined with cyclophosphamide.

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Neutral Polysaccharide from Panax notoginseng enhanced cyclophosphamide antitumor efficacy in hepatoma H22-bearing mice

10.21203/rs.3.rs-20231/v1 ◽

2020 ◽

Author(s):

Yanhong Liu ◽

Huayan Qin ◽

Yuanyuan Zhong ◽

Shuang Li ◽

Huajing Wang ◽

...

Keyword(s):

Exchange Resin ◽

Panax Notoginseng ◽

Ion Exchange Resin ◽

Inhibition Rate ◽

Resin Column ◽

Tumor Inhibition ◽

Tumor Bearing ◽

Crude Polysaccharide ◽

Fast Flow ◽

Tumor Inhibition Rate

Abstract Background: Our previous studies demonstrated that the administration of crude Polysaccharide from Panax notoginseng (CPPN) can effectively prolong the lifespan of tumor-bearing mice via boosting host immune system as well as a weak cytotoxicity against hepatocellular carcinoma (HCC). In the present study, Neutral Polysaccharide (NPPN) were further purified from crude polysaccharide isolated from panax notoginseng. The effects of NPPN on the immune function and hematopoietic function of mice with low immunity and myelosuppression induced by cyclophosphamide (CTX) were investigated. The effect of NPPN combined with CTX on the tumor inhibition rate of the H22 tumor-bearing mice and the impact of NPPN on the proliferation of H22 liver cancer cells in vitro were investigated.Methods: CPPN was obtained by water extraction and alcohol precipitation method, and further purified by DEAE Sepharose Fast Flow ion exchange resin column. NPPN was added to the immunosuppressed with myelosuppression mice induced by CTX. Thymus index, spleen index, lymphocyte proliferation stimulation index by adding of concanavalin A, determination of serum hemolysin, NK cell activity assay, mice carbon clearance experiment, blood count tests were detected. The tumor inhibition rate of the H22 tumor-bearing mice treated with NPPN combined with CTX was recorded.Results: NPPN and 4 kinds of acid polysaccharide from Panax notoginseng (APPN) were successfully isolated from the CPPN by DEAE Sepharose Fast Flow ion exchange resin column. NPPN inhibited the growth of H22 cells and significantly increase the tumor inhibition rate of the H22 tumor-bearing mice combined with CTX. The elevation of the cellular and humoral immunity levels as well as a variety of blood count tests indicators of immunosuppressive with myelosuppression mice may contribute to the antitumor activity of NPPN.Conclusion: NPPN has potential antitumor activity for the treatment of liver cancer combined with cyclophosphamide.

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Neutral Polysaccharide from Panax notoginseng enhanced cyclophosphamide antitumor efficacy in hepatoma H22-bearing mice

10.21203/rs.3.rs-20231/v3 ◽

2021 ◽

Author(s):

Yanhong Liu ◽

Huayan Qin ◽

Yuanyuan Zhong ◽

Shuang Li ◽

Huajing Wang ◽

...

Keyword(s):

Exchange Resin ◽

Panax Notoginseng ◽

Ion Exchange Resin ◽

Inhibition Rate ◽

Resin Column ◽

Tumor Inhibition ◽

Tumor Bearing ◽

Crude Polysaccharide ◽

Fast Flow ◽

Tumor Inhibition Rate

Abstract Background: Our previous studies demonstrated that the administration of crude Polysaccharide from Panax notoginseng (CPPN) can effectively prolong the lifespan of tumor-bearing mice via boosting the host immune system as well as weak cytotoxicity against hepatocellular carcinoma (HCC). In the present study, Neutral Polysaccharide (NPPN) were further purified from crude polysaccharide isolated from panax notoginseng. The effects of NPPN on the immune function and hematopoietic function of mice with low immunity and myelosuppression induced by cyclophosphamide (CTX) were investigated. The effect of NPPN combined with CTX on the tumor inhibition rate of the H22 tumor-bearing mice and the impact of NPPN on the proliferation of H22 liver cancer cells in vitro were investigated.Methods: CPPN was obtained by water extraction and alcohol precipitation method, and further purified by DEAE Sepharose Fast Flow ion exchange resin column. NPPN was added to the immunosuppressed with myelosuppression mice induced by CTX. Thymus index, spleen index, lymphocyte proliferation stimulation index by adding of concanavalin A, determination of serum hemolysin, NK cell activity assay, mice carbon clearance experiment, blood count tests were detected. The tumor inhibition rate of the H22 tumor-bearing mice treated with NPPN combined with CTX was recorded.Results: NPPN and 4 kinds of acid polysaccharide from Panax notoginseng (APPN) were successfully isolated from the CPPN by DEAE Sepharose Fast Flow ion exchange resin column. NPPN inhibited the growth of H22 cells and significantly increase the tumor inhibition rate of the H22 tumor-bearing mice combined with CTX. The elevation of the cellular and humoral immunity levels as well as a variety of blood count tests indicators of immunosuppressive with myelosuppression mice may contribute to the antitumor activity of NPPN.Conclusion: NPPN has a potential antitumor activity for the treatment of liver cancer combined with cyclophosphamide.

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A Low Dose of Hydrous Icaritin Nano-Formulation with Remarkable Efficacy and Tumor Targeting in Cancer Therapy

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2021.3176 ◽

2021 ◽

Vol 17 (10) ◽

pp. 2003-2013

Author(s):

Jingxin Fu ◽

Yian Wang ◽

Haowen Li ◽

Likang Lu ◽

Hui Ao ◽

...

Keyword(s):

Cancer Therapy ◽

Tumor Targeting ◽

Low Dose ◽

Drug Loading ◽

Tumor Therapy ◽

High Dose ◽

Inhibition Rate ◽

Tumor Inhibition ◽

Nano Formulation ◽

Tumor Inhibition Rate

Background: The use of chemotherapeutic drugs is restricted in the tumor-therapy because of the severely toxic and side effects among most important factors. The active herbal extracts are always used as a high dose while in the tumortherapy to achieve good anti-tumor effects. Hydrous icaritin has a high activity while there are few existing dosage forms as a result of low solubility in water and poor bioavailability. Results: The prepared hydrous icaritin nanorods (DP-HICT NRs) using mPEG2000-DSPE as a stabilizer, presented a narrow distribution of particle size with of 217 nm and a properly high drug-loading content of approximately 65.3±1.5%. A low dose of hydrous icaritin nano-formulation shows remarkable efficacy in cancer therapy (tumor inhibition rate: 61.36±10.80%) compared with the same dose of Paclitaxel injection (tumor inhibition rate: 66.80±4.43%), which approved as medicaments. Not only that, DP-HICT NRs can escape the clearance of the immune system and enhance targeting ability to the tumor site with only one excipient and such a low dose. Conclusions: This kind of nanoparticles contain a low dose of HICT used mPEG2000-DSPE as a stabilizer, while can achieve good tumor targeting as some active targeting agents and an anti-tumor effect as the PTX injection. There are broad prospects in drug safety, anti-tumor efficacy and even prognosis.

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Separation of Manganese, Cobalt, Copper, Zinc, and Magnesium By Using an Anion Exchange Resin

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/50.4.783 ◽

1967 ◽

Vol 50 (4) ◽

pp. 783-786

Author(s):

Eugene A Breatjlt

Keyword(s):

Ion Exchange ◽

Anion Exchange ◽

Exchange Resin ◽

Anion Exchange Resin ◽

Ion Exchange Resin ◽

Acid Solutions ◽

Resin Column ◽

Hydrochloric Acid Solutions ◽

Copper Zinc ◽

Resin Separation

Abstract The metals Mn, Mg, Co, Cu, and Zn were separated by using an anion exchange resin column and progressively less concentrated IIC1 solution as eluting agent. The position of Mg on an anion exchange resin, not previously reported in the literature, was determined. It has been shown that by using two different ion exchange resin columns, simple hydrochloric acid solutions as eluants, and polarographic or flame photometric determinations, 12 metals can be separately estimated in an aqueous solution containing about 100 /μg of each. It is recommended that the study of ion exchange resin separation of metals found in plants be continued.

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