Evaluation of Lens culinaris agglutinin-reactive α-fetoprotein for diagnosis of malignant tumors

2020 ◽  
Author(s):  
Qiong Lu ◽  
Jinxing Xia ◽  
Meijuan Zheng ◽  
Zhongwei Jia
Keyword(s):  
Liver Disease ◽  
Lens Culinaris ◽  
Malignant Tumors ◽  
Protein Microarrays ◽  
Serum Samples ◽  
Tumor Patients ◽  
Liver Malignancy ◽  
Lens Culinaris Agglutinin ◽  
Hepatic Diseases ◽  
Hepatocellular Carcinomas

Abstract Background: To assess the potential of AFP-L3% for the utility to diagnose malignant tumors.Methods: The AFP-L3 was concentrated from clinically-collected serum samples via the Hotgen Biotech glycosyl capture spin columns and then measured through the protein microarrays. The levels of AFP and AFP-L3 were detected by electrochemiluminescence immunoassay. In this retrospective study, 266 patients with the level of serum AFP-L3 over 1ng/ml were recruited from December 2014 through April 2019,Among them, 155 patients were clinically diagnosed/confirmed with malignant tumors, including 101 hepatocellular carcinomas, 47 stomach malignant tumors, and 7 other malignant tumors; and the rest of 111 patients were nomalignant tumors.Results: Patients with serum AFP-L3 level of greater than 1ng/ml were mainly detected in hepatic diseases, including hepatocellular carcinoma, cirrhosis and chronic hepatitis. In patients with no tumors, the levels of serum AFP-L3 over 1ng/ml were only observed in liver disease. The levels of AFP-L3 in blood were substantially greater in patients with HCC. Among the malignant tumor patients with the level of serum AFP-L3 over 1ng/ml, HCC accounted for 60%, gastric cancer for nearly 40%. The AFP, AFP-L3 and AFP-L3% in blood were increased significantly in patients with liver malignancy, chronic liver disease and cirrhosis. However, the elevation of AFP-L3 and AFP-L3% in the malignant cohort was more evident than that in the nonmalignant counterpart.Conclusions: AFP-L3 is likely to contribute to differential diagnosis of HCC as well as other hepatic diseases, AFP-L3% is a reliable indicator for diagnosing benign and malignant tumors.

Reference change values for lens culinaris agglutinin-reactive α-fetoprotein and des-γ-carboxy prothrombin in patients with chronic hepatitis C

2012 ◽  
Vol 50 (5) ◽  
Author(s):  
Futoshi Kanke ◽  
Takashi Kumada ◽  
Hidenori Toyoda ◽  
Shinji Satomura
Keyword(s):  
Capillary Electrophoresis ◽  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Liquid Phase ◽  
Lens Culinaris ◽  
Binding Assay ◽  
Hcv Infection ◽  
Microchip Capillary Electrophoresis ◽  
Serum Samples ◽  
Lens Culinaris Agglutinin

AbstractTen patients with cirrhosis due to hepatitis C virus (HCV) infection and without HCC were enrolled in the study. Serum samples were collected at 14-day intervals, and 10 samples in total were obtained for each patient. AFP-L3 and DCP levels were measured by microchip capillary electrophoresis and liquid-phase binding assay. Intra-individual (CVThe CVIncreases in values for AFP-L3 and DCP within 68.3% and 58.5% may be biological variations. Clinician should take these variations into consideration for the management of patients with HCV infection under surveillance of HCC.

scholarly journals Nanomaterials and hepatic disease: toxicokinetics, disease types, intrinsic mechanisms, liver susceptibility, and influencing factors

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ting Sun ◽  
Yiyuan Kang ◽  
Jia Liu ◽  
Yanli Zhang ◽  
Lingling Ou ◽  
...  
Keyword(s):  
Liver Disease ◽  
Liver Cancer ◽  
Metabolic Disorders ◽  
Hepatic Disease ◽  
Target Organ ◽  
Hepatic Diseases ◽  
Toxicological Studies ◽  
Main Target ◽  
Potential Risks ◽  
Further Development

AbstractThe widespread use of nanomaterials (NMs) has raised concerns that exposure to them may introduce potential risks to the human body and environment. The liver is the main target organ for NMs. Hepatotoxic effects caused by NMs have been observed in recent studies but have not been linked to liver disease, and the intrinsic mechanisms are poorly elucidated. Additionally, NMs exhibit varied toxicokinetics and induce enhanced toxic effects in susceptible livers; however, thus far, this issue has not been thoroughly reviewed. This review provides an overview of the toxicokinetics of NMs. We highlight the possibility that NMs induce hepatic diseases, including nonalcoholic steatohepatitis (NASH), fibrosis, liver cancer, and metabolic disorders, and explore the underlying intrinsic mechanisms. Additionally, NM toxicokinetics and the potential induced risks in the livers of susceptible individuals, including subjects with liver disease, obese individuals, aging individuals and individuals of both sexes, are summarized. To understand how NM type affect their toxicity, the influences of the physicochemical and morphological (PCM) properties of NMs on their toxicokinetics and toxicity are also explored. This review provides guidance for further toxicological studies on NMs and will be important for the further development of NMs for applications in various fields.

Relationship between Lens culinaris agglutinin-reactive alpha-fetoprotein and pathologic features of hepatocellular carcinoma

2005 ◽  
Vol 25 (4) ◽  
pp. 848-853 ◽  
Author(s):  
Toshifumi Tada ◽  
Takashi Kumada ◽  
Hidenori Toyoda ◽  
Seiki Kiriyama ◽  
Yasuhiro Sone ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Lens Culinaris ◽  
Alpha Fetoprotein ◽  
Lens Culinaris Agglutinin ◽  
Pathologic Features

Factors Influencing the Reaction of α1-Fetoprotein with Concanavalin A and Lens Culinaris Agglutinin in Crossed Affinoimmunoelectrophoresis

1992 ◽  
Vol 38 (8) ◽  
pp. 1418-1424 ◽  
Author(s):  
D Magne ◽  
N Seta ◽  
D Lebrun ◽  
G Durand ◽  
D Durand
Keyword(s):  
Concanavalin A ◽  
Lens Culinaris ◽  
Biological Fluids ◽  
Con A ◽  
Cellular Origin ◽  
Lens Culinaris Agglutinin ◽  
Lentil Lectin ◽  
Wide Range ◽  
Minimal Quantity ◽  
Antibody Ratio

Abstract Concanavalin A (Con A) and lentil lectin (LCA) analysis of alpha-fetoprotein (AFP) glycosylation heterogeneity is used in a variety of clinical situations. We studied the influence of analytical conditions on the separation of AFP glycoforms by using lectin-crossed affinoimmunoelectrophoresis, regardless of the AFP concentration, which can vary over a wide range in biological fluids. We defined the optimal concentration of Con A (2 g/L) and LCA (0.35 g/L) in the first-dimension gel, together with the optimum antigen (AFP)/antibody ratio in the second-dimension gel. The presence of protein in the diluent used for AFP samples was found to change the shape of crossed affinoimmunoelectrophoresis patterns without changing the percentage composition of AFP fractions. The within-run CV was less than 4% for both lectins, and the between-run CV was less than 6.3%. The minimal quantity of AFP that provided a visible pattern with both lectins was 4 ng, corresponding to 50 microL of an 80 micrograms/L AFP sample. These technical conditions allow the cellular origin of AFP to be determined, regardless of the concentration in the sample. Typical AFP lectin patterns of secreting tumors are compared with fetal and cord serum AFP.

Sign in / Sign up

Export Citation Format

Share Document