Soil Microbial Functional Diversity Responses to Different Vegetation Types in the Heilongjiang Zhongyangzhan Black-billed Capercaillie Nature Reserve

Abstract Purpose: The soil microbial community is an important bioactive component of terrestrial ecosystems. Its structural and functional diversity directly affects carbon and nitrogen processes. This study aimed to investigate the variations in the functional diversity of soil microbial communities in forests with different types of vegetation. Methods: We selected three typical vegetation types, larch (LG), black birch (BD), and larch and black birch mixed (LGBD) forests, located in the Heilongjiang Zhongyangzhan Black-billed Capercaillie Nature Reserve. The Biolog-Eco microplate technology was selected to perform these analyses. Result: Our results showed clear differences between microorganisms in the three typical forests. The average well-colour development (AWCD) change rate gradually increased with incubation time. The BD type had the highest AWCD value, followed by LGBD; the LG forest type had the lowest value. The difference in the soil microbial alpha diversity index between BD and LG was significant. A principal component analysis showed that PC1 and PC2 respectively explained 62.77% and 13.3% of the variance observed. The differences in the soil microbial carbon-source utilization patterns under different vegetation types were mainly caused by esters and carbohydrates. Redundancy analysis showed that soil microbial functional diversity was strongly affected by soil physicochemistrical properties (e.g. organic carbon, total nitrogen, and pH). Conclusion: These results provide a reference for further exploring the relationship between forest communities and soil microbes during the process of forest succession.

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Soil microbial functional diversity responses to different vegetation types in the Heilongjiang Zhongyangzhan Black-billed Capercaillie Nature Reserve

Annals of Microbiology ◽

10.1186/s13213-021-01638-4 ◽

2021 ◽

Vol 71 (1) ◽

Author(s):

Xiaohong Weng ◽

Jiayi Li ◽

Xin Sui ◽

Mengsha Li ◽

Weiping Yin ◽

...

Keyword(s):

Functional Diversity ◽

Nature Reserve ◽

Forest Type ◽

Diversity Index ◽

Soil Microbial Communities ◽

Principal Component ◽

Terrestrial Ecosystems ◽

Vegetation Types ◽

Microbial Functional Diversity ◽

Soil Microbial

Abstract Purpose The soil microbial community is an important bioactive component of terrestrial ecosystems. Its structural and functional diversity directly affects carbon and nitrogen processes. This study aimed to investigate the variations in the functional diversity of soil microbial communities in forests with different types of vegetation. Methods We selected three typical vegetation types, larch (LG), black birch (BD), and larch and black birch mixed (LGBD) forests, located in the Heilongjiang Zhongyangzhan Black-billed Capercaillie Nature Reserve. The Biolog-Eco microplate technology was selected to perform these analyses. Result Our results showed clear differences between microorganisms in the three typical forests. The average well colour development (AWCD) change rate gradually increased with incubation time. The BD type had the highest AWCD value, followed by LGBD; the LG forest type had the lowest value. The difference in the soil microbial alpha diversity index between BD and LG was significant. A principal component analysis showed that PC1 and PC2 respectively explained 62.77% and 13.3% of the variance observed. The differences in the soil microbial carbon-source utilisation patterns under different vegetation types were mainly caused by esters and carbohydrates. Redundancy analysis showed that soil microbial functional diversity was strongly affected by soil physicochemistrical properties (e.g. organic carbon, total nitrogen and pH). Conclusion These results provide a reference for further exploring the relationship between forest communities and soil microbes during the process of forest succession.

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Evaluation of functional diversity of soil microbial communities – a case study

Plant Soil and Environment ◽

10.17221/4074-pse ◽

2011 ◽

Vol 50 (No. 4) ◽

pp. 141-148 ◽

Cited By ~ 7

Author(s):

J. Hofman ◽

J. Švihálek ◽

I. Holoubek

Keyword(s):

Microbial Communities ◽

Functional Diversity ◽

Diversity Index ◽

Soil Microbial Communities ◽

Natural Area ◽

Multivariate Statistical ◽

Soil Microbial ◽

Routine Monitoring ◽

Best Parameter

In our case study, we measured the functional diversity of the microbial communities of twelve soils from the small natural area to assess if this assay is suitable for routine monitoring of soil biological quality. We found the BIOLOG assay meets especially practical benefits in routine monitoring of soils being simple and quick assay. However, we confirmed the ambiguity about the most appropriate analysis of the BIOLOG multivariate data and about the best parameter, which can be derived from the assay. The different analyses of the data were examined and various parameters derived from the BIOLOG assay were comparatively used to contribute to the discussion of how the data should be evaluated. We showed that not-normalized raw absorbances or trapezoid areas should be used for calculation of diversity index if the inoculum was standardized. There was no single answer to what parameter provided more correct results in the multivariate statistical analysis. Evaluating at least one not-normalized (e.g. trapezoid area) and one normalized parameter (e.g. absorbances read in fixed AWCD) was strongly suggested keeping in mind that they reveal different information.

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Soil Microbial Functional Diversity Responses to Different Revegetation Types in Baishilazi Nature Reserve

Polish Journal of Environmental Studies ◽

10.15244/pjoes/99100 ◽

2019 ◽

Vol 28 (5) ◽

pp. 3675-3686 ◽

Cited By ~ 1

Author(s):

Jiaojiao Deng ◽

Yongbin Zhou ◽

Xuejiao Bai ◽

Jiyao Luo ◽

You Yin ◽

...

Keyword(s):

Functional Diversity ◽

Nature Reserve ◽

Microbial Functional Diversity ◽

Soil Microbial

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Soil microbial functional diversity along an elevation gradient in Northwestern Caucasus

10.5194/egusphere-egu2020-21272 ◽

2020 ◽

Author(s):

Kristina Ivashchenko ◽

Alexandra Seleznyova ◽

Sofia Sushko ◽

Anna Zhuravleva ◽

Alexander Tronin ◽

...

Keyword(s):

Functional Diversity ◽

Plant Diversity ◽

Altitudinal Gradient ◽

Diversity Index ◽

Elevation Gradient ◽

Distribution Patterns ◽

Microbial Functional Diversity ◽

Soil Microbial ◽

Northwestern Caucasus ◽

Edaphic Properties

In contrast to taxonomic diversity of soil microbiome, the distribution patterns of functional diversity for various ecosystems, including along an altitudinal gradient, is poorly understood. Consequently, the study focuses on finding out the spatial distribution features of microbial functional diversity in mountainous soils along elevation through forests and meadows ecosystems. We hypothesized that soil microbial functional diversity is increasing along the altitudinal gradient in conjunction with plant diversity. In Northwestern Caucasus (Karachay-Cherkess Republic, Russia) the north-eastern mountain slope was studied across mixed, fir and deciduous forests, subalpine and alpine meadows located from 1260 to 2480 m above sea level. Twelve plots (0.25 m2 each) were randomly chosen within each ecosystem (total 60). Plant species composition and Shannon plant diversity index (H) were assessed for the plots. Topsoil samples (0-10 cm) were taken from the plots in August for assessment microbial functional diversity through community level physiological profile (CLPP). It was determined by microbial respiration response on amino, carboxylic, phenolic acids and carbohydrates (MicroResp). Shannon's functional diversity index based on the CLPP (HCLPP) was calculated. Edaphic properties as moisture, temperature, pH, total carbon (C) and nitrogen (N) contents were determined as possible drivers of CLPP. As expected, plant diversity was increased along the elevation gradient with the lowest H value in the mixed forest (0.6) and the highest &#8211; in the alpine meadow (1.9). The HCLPP did not differ among studied ecosystems and reached on average 2.4 for each. Microbial respiration response on amino acids was mainly contributed to dissimilarities between studied ecosystems and increased on average by 1.3 times with elevation from mixed to fir and deciduous forests. Along this elevation row, the soil N content was the most significant driver compared to other edaphic properties. Among subalpine and alpine meadows the differences between microbial responses on studied carbon substrates were not found.Considering that elevation didn&#8217;t contribute to distribution patterns of soil HCLPP at the inter-ecosystems level, consequently, the hypothesis of our study was rejected. Plant diversity was not related to HCLPP as expected. Meanwhile, the distribution patterns of soil microbial community, utilizing amino acids, along the altitudinal gradient was found.The current research was financially supported by RFBR No 20-34-70121

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Unravelling the Functional Diversity of the Soil Microbial Community of Chinese Fir Plantations of Different Densities

Forests ◽

10.3390/f9090532 ◽

2018 ◽

Vol 9 (9) ◽

pp. 532 ◽

Cited By ~ 6

Author(s):

Chaoqun Wang ◽

Lin Xue ◽

Yuhong Dong ◽

Yihui Wei ◽

Ruzhen Jiao

Keyword(s):

Microbial Community ◽

Microbial Communities ◽

Functional Diversity ◽

Soil Microbial Community ◽

Diversity Index ◽

Carbon Sources ◽

Soil Microbial Communities ◽

Stand Density ◽

Chinese Fir ◽

Soil Microbial

The structure and function of forest ecosystems are directly or indirectly affected by their stand density. However, what effect the density of Chinese fir plantations has on the functional diversity of the soil microbial community remains unclear. The microbial metabolic functional diversity of soils sampled at the topsoil (0–20 cm) of 35-year-old Chinese fir plantations of five initial densities (D1: 1667 stems∙hm−2, D2: 3333 stems∙hm−2, D3: 5000 stems∙hm−2, D4: 6667 stems∙hm−2, and D5: 10,000 stems∙hm−2) was studied by using Biolog ECO technology. The results showed that the soil pH, oxidizable organic carbon (SOOC), available N (AN), available P (AP), and available K (AK) contents all showed a gradual increase from D1 to D4 and a decrease from D4 to D5, while the number of culturable bacteria and total microorganisms, the average well color development (AWCD) values for the single carbon substrate and six types of carbon sources used by the microbial community, as well as the Shannon-Wiener diversity index (H’), Pielou evenness index (J), and McIntosh Diversity Index (U), were the opposite, suggesting that low-densities favored C and N mineralization and the nutrient cycle. The density of Chinese fir plantations had a significant effect on the use of carbohydrates, amino acids, carboxylic acids, and phenolic acids by the soil microbial community, but it had no significant effect on the use of polymers (p < 0.05). Principal component analysis (PCA) revealed that carbohydrates, polymers, and phenolic acids were sensitive carbon sources that caused differences in the metabolic functions of soil microbial communities in Chinese fir plantations. Redundancy analysis (RDA) showed that physicochemical factors have a significant influence on the metabolic function of soil microbial communities (RDA1 and RDA2 explained >85% variance). The changes in density affected the soil physicochemical properties, the composition, and the metabolic functional diversity of microbial communities in Chinese fir plantations, which is certainly useful for the stand density regulation of Chinese fir plantations.

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Genetic and functional diversity of soil bacteria and fungi from different microhabitats in a karst region in Southern China

Journal of Cave and Karst Studies ◽

10.4311/2020mb0106 ◽

2021 ◽

Vol 83 (3) ◽

pp. 120-132

Author(s):

Xinru Li ◽

Min Zhou ◽

Yidong Mi ◽

Haiyan Chen ◽

Hailei Su ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Functional Diversity ◽

Bacterial Community Structure ◽

Nature Reserve ◽

Vegetation Type ◽

Karst Region ◽

Vegetation Types ◽

Soil Microbial ◽

Bacteria And Fungi

Little is known about the difference between bacterial and fungal genetic and functional diversity in karst regions of south China. In this study, the genetic and functional diversity of bacteria and fungi in nine types of soil microenvironments in the karst region in Maolan National Nature Reserve in Guizhou were investigated by PCR-DGGE (Denaturing Gradient Gel Electrophoresis) and BIOLOG EcoPlates. Maolan National Nature Reserve is a UNESCO Biosphere Reserve and plays an important role in protecting the karst forest ecosystem and rare and endangered wild animals and plants in central Asia. The results showed that the diversity of both bacteria and fungi was high and the main factors influencing the diversity of bacteria and fungi were different. The bacterial community structure from different microhabitats under the same vegetation type had higher similarity than similar microhabitats in different vegetation types, which could indicate that the bacterial community structure was mainly controlled by vegetation. For fungi, similar microhabitat species under different vegetation types had higher similarities than different microhabitats species under the same vegetation type, which could indicate that the fungal community structure is mainly controlled by microhabitats. In addition, the metabolic patterns of similar microhabitats in different vegetation were different, while the metabolic patterns of different microhabitats in the same vegetation were not obviously different. In conclusion, the effect of vegetation types on soil microbial functional diversity was greater than that of microhabitats, and this difference was reflected by the different degrees of influence on soil microbial genetic diversity and community structure.

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Effect of Coarse Woody Debris on Soil Microbial Functional Diversity Following a Huge Ice Storm in South China

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.2649 ◽

2012 ◽

Vol 550-553 ◽

pp. 2649-2654

Author(s):

Yu Duan Ou ◽

Tian Wu ◽

Zhi Yao Su

Keyword(s):

Functional Diversity ◽

Coarse Woody Debris ◽

Woody Debris ◽

Soil Microbial Communities ◽

Soil Nutrient ◽

Positive Contribution ◽

Ice Storm ◽

Microbial Functional Diversity ◽

Soil Microbial ◽

Decay Class

Soil microbe’s response to coarse woody debris (CWD) was assessed in two contrasting stands. The volume, percent cover and weight of CWD in the damaged stand were significantly much higher than the undamaged stand. Redundancy Analysis showed that CWD and soil nutrient had a significant effect on soil microbial community (p < 0.005). Decay class of CWD had the greatest influence on soil microbial communities, and soil nutrient was next to it. With increasing decay class, CWD was favorable for soil microbial functional diversity. Alkali-hydrolyzable nitrogen, available phosphorus and available potassium showed a positive correlation with decay class and made a positive contribution on soil microbial functional diversity. Stands without ice storm impact temporarily had higher soil nutrient content than those suffered severe damage at the early stage after ice storm. Soil microbial functional diversity had no difference between both slopes. Findings reflected that there were other factors affecting the soil microbes in forest ecosystem except for the CWD decomposition and soil nutrient.

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Terrestrial ecosystems

10.1093/oso/9780198767220.003.0014 ◽

2018 ◽

Author(s):

Pierre Taberlet ◽

Aurélie Bonin ◽

Lucie Zinger ◽

Eric Coissac

Keyword(s):

Microbial Communities ◽

Functional Diversity ◽

Soil Microbial Communities ◽

Terrestrial Ecosystems ◽

Single Species ◽

Freshwater Ecology ◽

Species Detection ◽

Soil Microbial

Chapter 14 “Terrestrial ecosystems” focuses on the use of eDNA analysis for the study of terrestrial organisms, especially those found in or associated with soil. While eDNA-based analyses have rapidly gained momentum in the freshwater ecology community, first for single-species detection and more recently for diversity surveys, their success has been less immediate among terrestrial ecologists. Soil microbiologists are a notable exception, as they quickly realized that targeting DNA directly in the environment could free them from cultivating microorganisms prior to any community census. This chapter first addresses the particularities of detectability, persistence, and mobility of eDNA in soil. Then, it revisits several remarkable studies dealing with the characterization of plant, earthworm, or soil microbial communities, as well as soil functional diversity. Finally, Chapter 14 reviews one of the most fascinating opportunities offered by eDNA metabarcoding (i.e., the possibility to carry out multitaxa diversity surveys).

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