Microbial Diversity and Ecosystem Functioning in Deadwood of Black Pine of a Temperate Forest

The present study provides a deeper insight on variations of microbial abundance and community composition concerning specific environmental parameters related to deadwood decay, focusing on a mesocosm experiment conducted with deadwood samples from black pine of different decay classes. The chemical properties and microbial communities of deadwood changed over time. The total carbon percentage remained constant in the first stage of decomposition, showing a significant increase in the last decay class. The percentage of total nitrogen and the abundances of nifH harbouring bacteria significantly increased as decomposition advanced, suggesting N wood-enrichment by microbial N immobilization and/or N2-fixation. The pH slightly decreased during decomposition and significantly correlated with fungal abundance. CO2 production was higher in the last decay class 5 and positively correlated with bacterial abundance. Production of CH4 was registered in one sample of decay class 3, which correlates with the highest abundance of methanogenic archaea that probably belonged to Methanobrevibacter genus. N2O consumption increased along decomposition progress, indicating a complete reduction of nitrate compounds to N2 via denitrification, as proved by the highest nosZ gene copy number in decay class 5. Conversely, our results highlighted a low involvement of nitrifying communities in deadwood decomposition.

Is the modern-day dieback of yellow-cedar unprecedented?

In Southeast Alaska, many stands of yellow-cedar (Callitropsis nootkatensis D. Don; Oerst. ex D.P. Little; hereafter: ‘YC’) contain numerous standing, dead snags. Snag-age estimates based on morphology have been used to support the interpretation that a warming climate after ca. 1880 triggered unprecedented YC dieback. Here we present new estimates of YC snag longevity by cross-dating 61 snags with morphologies that suggest they stood dead for extended periods. All but four of these snags have lost their outermost rings to decay, so we estimate when they died using a new method based on wood-ablation rates measured in six living trees that display partial cambial dieback. Results indicate that ~59% of YC snags that lost their branches to decay (Class 5 snags) have remained standing for > 200 years, and some for as long as 450 years (snag longevity mean ± SD: 233 ± 92 years). These findings, along with supporting evidence from historical photos, dendrochronology, and snag-morphology surveys in the published literature suggest that episodes of YC dieback also occurred before 1880 and before significant anthropogenic warming began. The roles played by climate change in these earlier dieback events remain to be further explored.

Long-term effects of timber harvesting on coarse woody debris dynamics and marten habitat

At a long-term experimental trial in northern British Columbia, Canada, I analysed the impact of varying amounts of timber retention: 0% (clearcut), 40%, 70%, 100% (unharvested) on CWD volume, decay class, and inputs from windthrow over 27 years. I used attributes (diameter, length, decay class, and height above the ground) known to be favourable to marten to create an index for assessing the impact of harvesting intensity on CWD habitat features. I then used airborne laser scanning (ALS) to predict CWD volume, habitat value, and piece attributes over the landscape. Stands with 70% retention had CWD attributes that resulted in CWD habitat features similar to unharvested stands. Clearcuts contained pieces that were smaller, more decayed, and closer to the ground, which contributed less valuable habitat, compared to stands with higher retention. Windthrown trees were the majority of CWD inputs and volume change was positively related to percent retention.

Coarse Woody Debris’ Invertebrate Community is Affected Directly by Canopy Type and Indirectly by Thinning in Mixed Scots Pine—European Beech Forests

Research Highlights: Thinning and tree species alter the forest floor microclimate by modifying canopy cover, radiation, wind, and humidity. Thus, forest management can directly influence the edaphic mesofauna responsible for decomposing coarse woody debris (CWD). Background and Objectives: This research was carried out in the Southwestern Pyrenees Mountains (Northern Spain) and aimed to determine the influence of forest thinning and canopy type (pure Pinus sylvestris L. or a mix of P. sylvestris and Fagus sylvatica L.) on CWD colonization by edaphic fauna. Materials and Methods: CWD samples were collected belonging to intermediate and advanced decomposition stages, approximately 10 cm long and 5 cm in diameter. Using a design of three thinning intensities (0%, 20%, and 40% of basal area removed), with three replications per treatment (nine plots in total), four samples were taken per plot (two per canopy type) to reach 36 samples in total. Meso- and macrofauna were extracted from CWD samples with Berlese–Tullgren funnels, and individuals were counted and identified. Results: 19 taxonomic groups were recorded, the most abundant being the mesofauna (mites and Collembola). Mixed canopy type had a significant positive influence on richness, whereas advanced decay class had a positive significant influence on total abundance and richness. In addition, there were non-significant decreasing trends in richness and abundance with increasing thinning intensity. However, interactions among thinning intensity, canopy type, and decay class significantly affected mesofauna. Furthermore, some taxonomic groups showed differential responses to canopy type. CWD water content was positively correlated with total invertebrate abundance and some taxonomic groups. Our results suggest that stand composition has the potential to directly affect invertebrate communities in CWD, whereas stand density influence is indirect and mostly realized through changes in CWD moisture. As mesofauna is related to CWD decomposition rates, these effects should be accounted for when planning forest management transition from pure to mixed forests.

The effects of variable retention forestry on coarse woody debris dynamics and concomitant impacts on American marten habitat after 27 years

Coarse woody debris (CWD) in the form of logs, downed wood, stumps and large tree limbs is an important structural habitat feature for many small mammal species, including the American marten (Martes americana). At a long-term experimental trial in northern temperate hemlock-cedar forests of British Columbia, Canada, we analysed the impact of varying amounts of overstory basal area retention: 0% (clearcut), 40%, 70%, and 100% (unharvested) on CWD volume, decay class, and inputs from windthrow over 27 years. We used CWD attributes (diameter, length, decay class, and height above the ground) known to be favourable for martens to create an index for assessing the impact of harvesting intensity on CWD habitat features. Stands with 70% retention had CWD attributes that resulted in CWD habitat features similar to unharvested stands. Clearcuts contained pieces that were smaller, more decayed, and closer to the ground, which contributed to a habitat that was less valuable, compared with stands that had higher retention. Over the 27-year period, windthrown trees were the majority of CWD inputs, and volume change was positively related to percent retention. Our results highlight that forest management influences CWD size and input dynamics over multiple decades, and the need for consideration of these impacts when undertaking long-term multiple-use forestry planning.

Incidence of Internal Decay in American Elms (Ulmus americana) Under Regular Fungicide Injection to Manage Dutch Elm Disease

Fungicide injection is regularly performed to prevent and manage Dutch elm disease (DED) of American elm (Ulmus americana). In an effort to better understand the effects of long-term fungicide injection on tree health, sonic tomography (SoT) and electrical-resistance tomography (ERT) were used to nondestructively determine the incidence and severity of internal decay in the lower trunk of American elms in suburban and urban settings. Overall, 253 sonic and electrical-resistance tomograms were generated from 210 American elms. Sampled trees were partitioned into two fungicide injection groups: (1) regular injection; and (2) irregular injection or no known history of injection. Among all American elms, the incidence of internal decay in the lower trunk was 30% (63/210) with a mean percent decay, as determined by SoT, of 39%. Based on Chi-square analysis, there were no significant differences in the frequency of elms with decay by injection history (P = 0.799). Mean percent decay was significantly different by dbh class (P = 0.005) and while linear regression demonstrated a positive correlation between percent decay and dbh, most of the variability went unexplained (R2 = 0.182). For elms with decay, there was a significantly higher frequency of trees in the lowest decay class (< 25% of the cross section) compared to the highest decay class (> 75% of the cross section). The results suggest that the wounding associated with regular fungicide injection does not increase the likelihood of internal decay and that American elms exhibit a low frequency and severity of internal decay.

Epixylic vegetation abundance, diversity, and composition vary with coarse woody debris decay class and substrate species in boreal forest

Although the importance of coarse woody debris (CWD) to understory species diversity has been recognized, the combined effects of CWD decay and substrate species on abundance and species diversity of epixylic vegetation have received little attention. We sampled a wide range of CWD substrate species and decay classes, as well as forest floors in fire-origin boreal forest stands. Percent cover, species richness, and evenness of epixylic vegetation differed significantly with both CWD decay class and substrate species. Trends in cover, species richness, and evenness differed significantly between nonvascular and vascular taxa. Cover, species richness, and species evenness of nonvascular species were higher on CWD, whereas those of vascular plants were higher on the forest floor. Epixylic species composition also varied significantly with stand ages, overstory compositions, decay classes, substrate species, and their interactions. Our findings highlight strong interactive influences of decay class and substrate species on epixylic plant communities and suggest that conservation of epixylic diversity would require forest managers to maintain a diverse range of CWD decay classes and substrate species. Because stand development and overstory compositions influence CWD decay classes and substrate species, as well as colonization time and environmental conditions in the understory, our results indicate that managed boreal landscapes should consist of a mosaic of different successional stages and a broad suite of overstory types to support diverse understory plant communities.

Influence of substrate features on distribution of polypores (Fungi: Basidiomycota) in central part of Peechi Vazhani Wildlife Sanctuary, Kerala, India

We examined the effect of substrate features like diameter, type and decay class on the distribution of polypores in the moist deciduous forests of Kerala. Plot based sampling and opportunistic sampling method were adopted to maximize the documentation of polypore fungal distribution. The highest number (2,861) of polypore fungal sporocarps has been recorded in host trees with 21-<30 cm diameter class. Among the substrate types, highest number of individuals (2,480) were observed on logs, living trees supported very few polypores. The newly emerged species during the monsoon season showed more association with decay class 2, the decay class association of some species remained unchanged during all the seasons.

Dead wood characteristics influencing macrofungi species abundance and diversity in Caspian natural beech (Fagus orientalis Lipsky) forests

<p><em>Aim of study</em>: This study aimed to examine the dead wood inhabiting macrofungi communities occurring on dead beech and hornbeam trees in Caspian forests.<strong></strong></p><p><em>Area of study</em>: The Kheiroud forest in the north of Iran.</p><p><em>Material and Methods</em>: Data from 205 sampling dead tree were analyzed by means of Generalized Linear Models (GLM) to test the effects of decay stage, DBH, Length or Height on macrofungi diversity. Additionally, tree species, dead wood size, log position, decay stage were used as predictor factors for the number of sporocarps species (NSS) as a fungal species richness and diversity in each dead log using analysis of variance</p><p><em>Main results</em>: The number of sporocarps species (NSS) varied in different dead wood size and decay classes. The different stages of decay and the different size classes of dead wood had significantly different species richness of macrofungi. Deadwood in the high-decayed stages contained the highest diversity of fungi. Most of fungi identified on both logs and snags belonged to Basidiomycetes and Ascomycetes. The highest value for richness and evenness indices calculated in large diameter dead wood in decay class III. The results indicated the size and decay class of dead wood describe the greatest variance of the model that means the highest number of sporocarps species inhabited on the large dead wood in advanced stage of decaying.</p><p><em>Research highlights</em>: Macrofungi diversity varied significantly across pieces of dead wood with downed logs, larger pieces, and wood in later stages of decay having the highest macrofungi diversity.</p><p> <strong>Keywords</strong>: Caspian forest; coarse woody debris; down woody debris; Iran.<strong></strong></p>