scholarly journals Development of Markers for Identification and Maker-Assisted Breeding of Xa7 Gene in Rice (Oryza sativa L.)

2021 ◽  
Author(s):  
Pengcheng Liu ◽  
Le Mei ◽  
Lumei He ◽  
Yiling Xu ◽  
Yiting Zhang ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Null Allele ◽  
Susceptible Variety ◽  
Breeding Value ◽  
Functional Markers ◽  
Dominant Marker ◽  
Rice Varieties ◽  
Rice Bacterial Blight ◽  
Molecular Maker ◽  
Specific Sequences

Abstract Utilization of resistance (R) genes to breed resistant cultivars is one of the most effective and economical approach to control rice bacterial blight (BB). Xa7, a dominant, broad-spectrum and durable BB-resistant gene, is an ideal gene resource to improve the resistance of rice varieties to bacterial blight, and this well-known gene with important breeding value has been cloned in our recent study. The isolation of Xa7 will facilitate its application in rice breeding by molecular maker-assisted selection (MAS). In this study, based on the specific sequences in the promoter of Xa7, a functional marker, named as MX7, was developed, which can effectively distinguish the dominant BB-resistant Xa7, the recessive BB-susceptible xa7 and the null allele from different rice varieties. Since MX7 is a dominant marker, it can't tell homozygous from heterozygous, a co-dominant marker closely linked to Xa7, named as M6, was developed simultaneously. After verified by amplification in numerous rice varieties and sequence alignment in RICE 3K database, it is proved that marker M6 is co-segregated with the Xa7 locus. In addition, the effectiveness and accuracy of the two markers were further validated by two F2 populations. Finally, the designed makers were effectively applied in MAS breeding to improve the BB-resistance of a susceptible variety. This study not only provides reliable functional markers for the identification of Xa7 gene in different rice materials, but also will contribute to the application of Xa7 gene in marker-assisted selection to breed rice varieties with durable disease resistance.

scholarly journals Transgenic Expression of the Functional Fragment Hpa110-42 of the Harpin Protein Hpa1 Imparts Enhanced Resistance to Powdery Mildew in Wheat

Plant Disease ◽  
2014 ◽  
Vol 98 (4) ◽  
pp. 448-455 ◽  
Author(s):  
Defu Wang ◽  
Yajun Wang ◽  
Maoqiang Fu ◽  
Shuyuan Mu ◽  
Bing Han ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Bacterial Blight ◽  
Fungal Species ◽  
Effective Control ◽  
Transgenic Expression ◽  
Rice Bacterial Blight ◽  
Harpin Protein ◽  
Transgenic Lines ◽  
Bacterial Blight Pathogen ◽  
Pathogen Populations

Powdery mildew, one of devastating diseases of wheat worldwide, is caused by Erysiphe graminis f. sp. tritici, a fungal species with constant population changes, which often poses challenges in disease management with host resistance. Transgenic approaches that utilize broad-spectrum resistance may limit changes of pathogen populations and contribute to effective control of the disease. The harpin protein Hpa1, produced by the rice bacterial blight pathogen, can induce resistance to bacterial blight and blast in rice. The fragment comprising residues 10 through 42 of Hpa1, Hpa110-42, is reportedly three- to eightfold more effective than the full-length protein. This study evaluated the transgenic expression of the Hpa110-42 gene for resistance to powdery mildew in wheat caused by E. graminis f. sp. tritici. Nine Hpa110-42 transgenic wheat lines were generated. The genomic integration of Hpa110-42 was confirmed, and expression of the transgene was detected at different levels in the individual transgenic lines. Following inoculation with the E. graminis f. sp. tritici isolate Egt15 in the greenhouse, five transgenic lines had significantly higher levels of resistance to powdery mildew compared with nontransformed plants. Thus, transgenic expression of Hpa110-42 conferred resistance to one isolate of E. graminis f. sp. tritici in wheat in the greenhouse.

scholarly journals Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21

1992 ◽  
Vol 236 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Pamela C. Ronald ◽  
Beng Albano ◽  
Rodante Tabien ◽  
Lleva Abenes ◽  
Kung-sheng Wu ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Bacterial Blight ◽  
Resistance Locus ◽  
Physical Analysis ◽  
Rice Bacterial Blight ◽  
Bacterial Blight Disease ◽  
Blight Disease

scholarly journals Estimation of Rice Yield Loss Using a Simple Linear Regression Model for Bacterial Blight Disease

2020 ◽  
Vol 23 (1) ◽  
pp. 73-79
Author(s):  
T H Ansari ◽  
M Ahmed ◽  
S Akter ◽  
M S Mian ◽  
M A Latif ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Yield Loss ◽  
Rice Variety ◽  
Severity Index ◽  
Susceptible Variety ◽  
Crop Growth ◽  
Grain Weight ◽  
Growth Stages ◽  
Crop Growth Stages ◽  
1000 Grain Weight

Field experiment was carried out in hot and humid summer (Transplanted Aus) season to realize the yield loss of a susceptible rice variety Purbachi inoculated with bacterial blight (BB). Treatments consist of BB inoculations at different crop growth stages like maximum tillering (MT), panicle initiation (PI), booting (Bt), flowering and heading stages differently including a control (no BB inoculation). Disease severity index (DSI) was measured at 14 days after inoculation (DAI) and harvest. Data on 1000-grain-weight and yield was recorded at harvest. Significant variation on DSI was observed among different BB inoculated crop growth stages. MT, PI and Boot stage inoculations showed similar (DSI 7.1-8.0) but higher DSI than flowering and heading stages inoculation (3.2-5.3) even control (0.00) at 14 DAI. However, all the treatments showed similar DSI 9.0 at harvest. Bacterial blight can affect the grain weight to some extent although it was insignificant among the treatments (0.1-4.5%). DSI showed negative correlation with 1000-grain weight (r=-0.77*) and similarly with the yield (r=-0.97**). The yield ranged from 2.4-3.4 t/ha among the treatments. The yield loss was observed 5.8-30.4% in the BB inoculated treatments. MT, PI and Boot stages inoculation affected the yield much resulting 21-30.4% yield loss. It could be concluded that a susceptible variety can be affected with significant yield loss up to 30.4% with severe outbreak of B B. A simple regression equation = 4.09-0.211X( = Yield, X = BB severity score) is suggested for the prediction of yield loss in susceptible variety in summer season. Bangladesh Rice j. 2019, 23(1): 73-79

scholarly journals Host-Pathogen Interaction in Rice-Bacterial Blight Pathosystem

2008 ◽  
Vol 48 (3) ◽  
Author(s):  
Debabrata Nayak ◽  
Lotan Bose ◽  
Pothireddy Reddy ◽  
Parsuram Nayak
Keyword(s):  
Bacterial Blight ◽  
Rice Bacterial Blight ◽  
Host Pathogen Interaction ◽  
Host Pathogen
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