The impact of intervention to the incidence of natural focus diseases during the outbreak of COVID-19 in Jiangsu Province, China

Abstract Doxorubicin (DOX) is a well-known chemotherapeutic drug for most malgnencies including breast cancer and leukemia whilst the usage of DOX is limited owing to its cardiotoxicity. The present study analyzed the effects of crocin on doxorubicin’s cardiotoxic efect in rat myocardium and searched their mechanistic interaction in the pathogenesis of DOX-induced myocardial toxicity. Forty rats were divided into four groups; (a) control (received normal saline as a dose of 1 ml/kg by ip for 15 days), (b) Crocin (received crocin as a dose of 40 mg/kg/24h by ip for 15 days), (c) DOX (received DOX as a dose of 2 mg/kg/48h by ip in six injection, cumulative dose 12 mg/kg), and (d) DOX+Crocin (received DOX as a dose of 2 mg/kg/48h by ip in six injection and crocin as a dose of 40 mg/kg/24h ip for 15 days). According to the present study, DOX administration caused significant increases in lipid indices (triglyseride, low-dencity lipoproteins and very low-dencity lipoproteins) as well as cardiac markers (Creatine kinase-muscle/brain and Cardiac Troponin I). Morever, DOX caused significant increases in oxidative stress parameters (malondialdehyde and total oxidant status) as well as decreases in antioxidant defense systems (glutathione, superoxide dismutase, catalase and total antioxidant status). The present study also demonstrated that co-administration of crocin with DOX significantly ameliorated the lipid profile and biochemical parameters in rats receiving DOX. The results were supported by histopathological and immunohistochemical evaluations. Taken together, our results reveal that crocin might be a cardioprotective agent in DOX treated patients for cancer.

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Antioxidant, anti-inflammatory and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats

10.21203/rs.3.rs-418902/v1 ◽

2021 ◽

Author(s):

Sara Asaad ABDULKAREEM ALJUMAILY ◽

Mehmet Demir ◽

Hulya Elbe ◽

Gurkan Yigitturk ◽

Yasemin Bicer ◽

...

Keyword(s):

Antioxidant Defense ◽

Troponin I ◽

Total Antioxidant Status ◽

Cardiac Markers ◽

Chemotherapeutic Drug ◽

Defense Systems ◽

Total Antioxidant ◽

Antioxidant Defense Systems ◽

Apoptotic Effects ◽

Oxidant Status

Abstract Doxorubicin (DOX) is a well-known chemotherapeutic drug for most malgnencies including breast cancer and leukemia whilst the usage of DOX is limited owing to its cardiotoxicity. The present study analyzed the effects of crocin on doxorubicin’s cardiotoxic efect in rat myocardium and searched their mechanistic interaction in the pathogenesis of DOX-induced myocardial toxicity. Forty rats were divided into four groups; (a) control (received normal saline as a dose of 1 ml/kg by ip for 15 days), (b) Crocin (received crocin as a dose of 40 mg/kg/24h by ip for 15 days), (c) DOX (received DOX as a dose of 2 mg/kg/48h by ip in six injection, cumulative dose 12 mg/kg), and (d) DOX + Crocin (received DOX as a dose of 2 mg/kg/48h by ip in six injection and crocin as a dose of 40 mg/kg/24h ip for 15 days). According to the present study, DOX administration caused significant increases in lipid indices (triglyseride, low-dencity lipoproteins and very low-dencity lipoproteins) as well as cardiac markers (Creatine kinase-muscle/brain and Cardiac Troponin I). Morever, DOX caused significant increases in oxidative stress parameters (malondialdehyde and total oxidant status) as well as decreases in antioxidant defense systems (glutathione, superoxide dismutase, catalase and total antioxidant status). The present study also demonstrated that co-administration of crocin with DOX significantly ameliorated the lipid profile and biochemical parameters in rats receiving DOX. The results were supported by histopathological and immunohistochemical evaluations. Taken together, our results reveal that crocin might be a cardioprotective agent in DOX treated patients for cancer.

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Are insulin-resistance and oxidative stress cause or consequence of aging

Experimental Biology and Medicine ◽

10.1177/1535370220929621 ◽

2020 ◽

Vol 245 (14) ◽

pp. 1260-1267

Author(s):

Sylwia Dzięgielewska-Gęsiak ◽

Dorota Stołtny ◽

Alicja Brożek ◽

Małgorzata Muc-Wierzgoń ◽

Ewa Wysocka

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Antioxidant Status ◽

Antioxidant Defense ◽

Total Antioxidant Status ◽

Thiobarbituric Acid ◽

Elderly Individuals ◽

Total Antioxidant ◽

Antioxidant Defense Systems ◽

Antioxidant Markers

Insulin resistance (IR) may be associated with oxidative stress and leads to cardiovascular disorders. Current research focuses on interplay between insulin-resistance indices and oxidant-antioxidant markers in elderly individuals with or without insulin-resistance. The assessment involved anthropometric data (weight, height, BMI, percentage of body fat (FAT)) and biochemical tests (glucose, lipids, serum insulin and plasma oxidant-antioxidant markers: Thiobarbituric Acid-Reacting Substances (TBARS), Cu,Zn-superoxide dismutase (SOD-1) and total antioxidant status). Insulin resistance index (IR) assuming a cut-off point of 0.3 allows to divides groups into: insulin sensitive group (InsS) IR < 0,3 ( n = 35, median age 69.0 years) and insulin-resistant group (InsR) IR ≥ 0.3 ( n = 51, median age 71.0 years). Lipids and antioxidant defense system markers did not differentiate the investigated groups. In the InsR elderly group, the FAT was increased ( P < 0.000003) and TBARS ( P = 0.008) concentration decreased in comparison with InsS group. A positive correlation for SOD-1 and total antioxidant status ( P < 0.05; r = 0.434) and a negative correlation for TBARS and age ( P < 0.05 with r = −0.421) were calculated in InsR individuals. In elderly individuals, oxidative stress persists irrespective of insulin-resistance status. We suggest that increased oxidative stress may be consequence of old age. An insulin action identifies those at high risk for atherosclerosis, via congruent associations with oxidative stress and extra- and intra-cellular antioxidant defense systems. Thus, we maintain that insulin-resistance is not the cause of aging. Impact statement Insulin resistance is associated with oxidative stress leading to cardiovascular diseases. However, little research has been performed examining elderly individuals with or without insulin-resistance. We demonstrate that antioxidant defense systems alone is not able to abrogate insulin action in elderly individuals at high risk for atherosclerosis, whereas the combined oxidant-antioxidant markers (thiobarbituric acid-reacting substances (TBARS), Cu,Zn-superoxide dismutase (SOD-1), and total antioxidant status (TAS)) might be more efficient and perhaps produce better clinical outcome. In fact, a decrease in oxidative stress and strong interaction between antioxidant defense can be seen only among insulin-resistant elderly individuals. This is, in our opinion, valuable information for clinicians, since insulin-resistance is considered strong cardiovascular risk factor.

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Paraoxonase and oxidative stress changes in left and right heart of exhaustively exercised rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2020-0332 ◽

2020 ◽

Author(s):

BADEGÜL SARIKAYA ◽

Metin Runa ◽

Duygu Dayanır ◽

Yasemin Gündüztepe ◽

Lamia Pınar

Keyword(s):

Oxidative Stress ◽

Troponin I ◽

Total Antioxidant Status ◽

Exhaustive Exercise ◽

Paraoxonase 1 ◽

Pon1 Activity ◽

Stress Changes ◽

Total Antioxidant ◽

Left And Right ◽

Oxidant Status

Exhaustive exercise can cause subclinical inflammation to heart, since it is an oxidative tissue that works continuously. The effect of exhaustive exercise on left and right ventricles (LVs, RVs) may be different. It is claimed that paraoxonase-1 (PON1), an antioxidant enzyme, has a cardioprotective effect on oxidative stress. Rats were separated as; non-exercised controls (Con), those euthanized immediately after (E-0) and 24 hours after exhaustive exercise (E-24). Cardiac troponin-I (cTnI), total antioxidant status (TAS), total oxidant status (TOS), PON1 activities and histological findings in LV and RV of the exhausted rats were evaluated. TAS and PON1 levels were lower in LVs compared to RVs of all groups. TOS levels were high in LVs compared to RVs of all groups. In LVs, TAS levels decreased significantly in E-0 group while PON1 activity decreased in E-0 and E-24 groups compared to controls. In LVs, TOS levels decreased significantly in E-0 and E-24 groups, but in RVs decrease was seen only in E-0 group. cTnI levels increased significantly in E-0 group, and decreased to control levels in E-24 group. Considering the histological and biochemical findings, exhaustive exercise affected the heart to the maximum during/just after exhaustion, and LV was influenced more than RV.

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Association between calcitriol and paricalcitol with oxidative stress in patients with hemodialysis

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000641 ◽

2020 ◽

pp. 1-8

Author(s):

Hasan Haci Yeter ◽

Berfu Korucu ◽

Elif Burcu Bali ◽

Ulver Derici

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Stress Index ◽

Total Oxidant Status ◽

Vitamin D Analog ◽

Total Antioxidant ◽

Oxidant Status ◽

And Oxidative Stress

Abstract. Background: The pathophysiological basis of chronic kidney disease and its complications, including cardiovascular disease, are associated with chronic inflammation and oxidative stress. We investigated the effects of active vitamin D (calcitriol) and synthetic vitamin D analog (paricalcitol) on oxidative stress in hemodialysis patients. Methods: This cross-sectional study was composed of 83 patients with a minimum hemodialysis vintage of one year. Patients with a history of any infection, malignancy, and chronic inflammatory disease were excluded. Oxidative markers (total oxidant and antioxidant status) and inflammation markers (C-reactive protein and interleukin-6) were analyzed. Results: A total of 47% (39/83) patients were using active or analog vitamin D. Total antioxidant status was significantly higher in patients with using active or analog vitamin D than those who did not use (p = 0.006). Whereas, total oxidant status and oxidative stress index were significantly higher in patients with not using vitamin D when compared with the patients who were using vitamin D preparation (p = 0.005 and p = 0.004, respectively). On the other hand, total antioxidant status, total oxidant status, and oxidative stress index were similar between patients who used active vitamin D or vitamin D analog (p = 0.6; p = 0.4 and p = 0.7, respectively). Conclusion: The use of active or selective vitamin D analog in these patients decreases total oxidant status and increases total antioxidant status. Also, paricalcitol is as effective as calcitriol in decreasing total oxidant status and increasing total antioxidant status in patients with chronic kidney disease.

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