Three-Dimensional Modeling and Simulation of Muscle Tissue Puncture Process

2021 ◽  
Author(s):  
zongkai lv ◽  
qinghua song ◽  
fan gao ◽  
zhanqiang liu ◽  
yi wan ◽  
...  
Keyword(s):  
Success Rate ◽  
Muscle Tissue ◽  
Clinical Medicine ◽  
Three Dimensional ◽  
Virtual Surgery ◽  
Three Dimensional Modeling ◽  
Simulation Results ◽  
High Degree ◽  
Puncture Model

Abstract Needle biopsy is an important part of modern clinical medicine. The puncture accuracy and sampling success rate of puncture surgery can be effectively improved through virtual surgery. Because fewer puncture existing three-dimensional(3D) model, it is impossible to guide the operation under complicated working conditions, which limits the development of virtual surgery. In this paper, 3D simulation of muscle tissue puncture process is studied. Firstly, the parameters of muscle tissue are measured. Considering the fitting accuracy and calculation speed, the M-R model is selected. Subsequently, an accurate 3D dynamic puncture model is established. The failure criterion is used to define the breaking characteristics of the muscle, and the bilinear cohesion model defines the breaking process. Experiments with different puncture speeds are carried out through the built in vitro puncture platform. The experimental results are compared with the simulation results. The accuracy of the model is verified by the high degree of agreement between the two curves. Finally, the model under different parameters is studied. Analyze the simulation results of different puncture depths and puncture speeds. The 3D puncture model can provide a more accurate model support for virtual surgery and help improve the success rate of puncture surgery.

scholarly journals Functional studies of twelve mutant V2 vasopressin receptors related to nephrogenic diabetes insipidus: molecular basis of a mild clinical phenotype.

1998 ◽  
Vol 9 (10) ◽  
pp. 1861-1872
Author(s):  
Y Ala ◽  
D Morin ◽  
B Mouillac ◽  
N Sabatier ◽  
R Vargas ◽  
...  
Keyword(s):  
Diabetes Insipidus ◽  
Nephrogenic Diabetes Insipidus ◽  
Three Dimensional ◽  
Urine Osmolality ◽  
Complete Loss ◽  
Loss Of Function ◽  
Hydrogen Bond Formation ◽  
Functional Studies ◽  
Three Dimensional Modeling

X-linked nephrogenic diabetes insipidus (NDI) is a rare disease with defective renal and extrarenal arginine vasopressin V2 receptor responses due to mutations in the AVPR2 gene in Xq28. To study the cause of loss of function of mutant V2 receptors, we expressed 12 mutations (N55H, L59P, L83Q, V88M, 497CC-->GG, deltaR202, I209F, 700delC, 908insT, A294P, P322H, P322S) in COS-7 cells. Eleven of these, including P322H, were characterized by a complete loss of function, but the mutation P322S demonstrated a mild clinical and in vitro phenotype. This was characterized by a late diagnosis without any growth or developmental delay and a significant increase in urine osmolality after intravenous 1-deamino[D-Arg8]AVP administration. In vitro, the P322S mutant was able to partially activate the Gs/adenylyl cyclase system in contrast to the other V2R mutants including P322H, which were completely inactive in this regard. This showed not only that Pro 322 is important for proper V2R coupling, but also that the degree of impairment is strongly dependent on the identity of the substituting amino acid. Three-dimensional modeling of the P322H and P322S mutant receptors suggested that the complete loss of function of the P322H receptor could be due, in part, to hydrogen bond formation between the His 322 side chain and the carboxyl group of Asp 85, which does not occur in the P322S receptor.

scholarly journals Constructing a Computer Model of the Human Eye Based on Tissue Slice Images

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Peishan Dai ◽  
Boliang Wang ◽  
Chunbo Bao ◽  
Ying Ju
Keyword(s):  
Computer Model ◽  
Geometric Model ◽  
Three Dimensional ◽  
Tissue Slice ◽  
Central Axis ◽  
Virtual Surgery ◽  
Three Dimensional Model ◽  
Human Eye ◽  
Eye Tissues

Computer simulation of the biomechanical and biological heat transfer in ophthalmology greatly relies on having a reliable computer model of the human eye. This paper proposes a novel method on the construction of a geometric model of the human eye based on tissue slice images. Slice images were obtained from an in vitro Chinese human eye through an embryo specimen processing methods. A level set algorithm was used to extract contour points of eye tissues while a principle component analysis was used to detect the central axis of the image. The two-dimensional contour was rotated around the central axis to obtain a three-dimensional model of the human eye. Refined geometric models of the cornea, sclera, iris, lens, vitreous, and other eye tissues were then constructed with their position and ratio relationships kept intact. A preliminary study of eye tissue deformation in eye virtual surgery was simulated by a mass-spring model based on the computer models developed.

scholarly journals Prediction of pharmacological activities from chemical structures with graph convolutional neural networks

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miyuki Sakai ◽  
Kazuki Nagayasu ◽  
Norihiro Shibui ◽  
Chihiro Andoh ◽  
Kaito Takayama ◽  
...  
Keyword(s):  
Structural Information ◽  
Three Dimensional ◽  
Structural Diversity ◽  
Amino Acid Sequences ◽  
Chemical Structures ◽  
Therapeutic Drugs ◽  
Complex Information ◽  
Antidepressant Effects ◽  
High Degree

AbstractMany therapeutic drugs are compounds that can be represented by simple chemical structures, which contain important determinants of affinity at the site of action. Recently, graph convolutional neural network (GCN) models have exhibited excellent results in classifying the activity of such compounds. For models that make quantitative predictions of activity, more complex information has been utilized, such as the three-dimensional structures of compounds and the amino acid sequences of their respective target proteins. As another approach, we hypothesized that if sufficient experimental data were available and there were enough nodes in hidden layers, a simple compound representation would quantitatively predict activity with satisfactory accuracy. In this study, we report that GCN models constructed solely from the two-dimensional structural information of compounds demonstrated a high degree of activity predictability against 127 diverse targets from the ChEMBL database. Using the information entropy as a metric, we also show that the structural diversity had less effect on the prediction performance. Finally, we report that virtual screening using the constructed model identified a new serotonin transporter inhibitor with activity comparable to that of a marketed drug in vitro and exhibited antidepressant effects in behavioural studies.

scholarly journals Electrophysiological analysis of healthy and dystrophic 3D bioengineered skeletal muscle tissues

2021 ◽  
Author(s):  
Christine T. Nguyen ◽  
Majid Ebrahimi ◽  
Penney M. Gilbert ◽  
Bryan Andrew Stewart
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cell ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Dystrophin Gene ◽  
Muscle System ◽  
Electrophysiological Analysis ◽  
Cytoskeletal Network ◽  
Muscle Tissues

Recently, methods for creating three-dimensional (3D) human skeletal muscle tissues from myogenic cell lines have been reported. Bioengineered muscle tissues are contractile and respond to electrical and chemical stimulation. In this study we provide an electrophysiological analysis of healthy and dystrophic 3D bioengineered skeletal muscle tissues. We focus on Duchenne muscular dystrophy (DMD), a fatal muscle disorder involving the skeletal muscle system. The dystrophin gene, which when mutated causes DMD, encodes for the Dystrophin protein, which anchors the cytoskeletal network inside of a muscle cell to the extracellular matrix outside the cell. Here, we enlist a 3D in vitro model of DMD muscle tissue, to evaluate an understudied aspect of DMD, muscle cell electrical properties uncoupled from presynaptic neural inputs. Our data shows that electrophysiological aspects of DMD are replicated in the 3D bioengineered skeletal muscle tissue model. Furthermore, we test a block co-polymer, poloxamer 188, and demonstrate capacity for improving the membrane potential in DMD muscle. Therefore, this study serves as the baseline for a new in vitro method to examine potential therapies directed at muscular disorders.

scholarly journals Trauma induced tissue survival in vitro with a muscle-biomaterial based osteogenic organoid system: a proof of concept study

2019 ◽  
Author(s):  
Tao He ◽  
Jörg Hausdorf ◽  
Yan Chevalier ◽  
Roland Manfred Klar
Keyword(s):  
Skeletal Muscle ◽  
Bone Tissue ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Good Alternative ◽  
Clinical Environment ◽  
In Vitro Systems ◽  
Osteogenic Gene Expression

Abstract Background The translation from animal research into the clinical environment remains problematic, as animal systems do not adequately replicate the human in vivo environment. Bioreactors have emerged as a good alternative that can reproduce part of the human in vivo processes at an in vitro level. However, in vitro bone formation platforms primarily utilizes stem cells only, with tissue based in vitro systems remaining poorly investigated. As such, the present pilot study explored the tissue behavior and cell survival capability within a new in vitro skeletal muscle tissue-based biomaterial organoid bioreactor system to maximize future bone tissue engineering prospects. Results Three dimensional printed β-tricalcium phosphate/hydroxyapatite devices were either wrapped in a sheet of rat muscle tissue or first implanted in a heterotopic muscle pouch that was then excised and cultured in vitro for up to 30 days. Devices wrapped in muscle tissue showed cell death by day 15. Contrarily, devices in muscle pouches showed angiogenic and limited osteogenic gene expression tendencies with consistent TGF-ß 1 , COL4A1 , VEGF-A , RUNX-2 , and BMP-2 upregulation, respectively. Histologically, muscle tissue degradation and fibrin release was seen being absorbed by devices acting possibly as a support for new tissue formation in the bioceramic scaffold that supports progenitor stem cell osteogenic differentiation.Conclusions These results therefore demonstrate that the skeletal muscle pouch-based biomaterial culturing system can support tissue survival over a prolonged culture period and represents a novel organoid tissue model that with further adjustments could generate bone tissue for direct clinical transplantations.

Biopolymers advances in Medical Sciences: An Editorial Review

2021 ◽  
Vol 15 (10) ◽  
pp. 2501-2502
Author(s):  
Taimoor Hassan ◽  
Xiaojia Huang ◽  
Sana Saeed
Keyword(s):  
Clinical Medicine ◽  
Three Dimensional ◽  
Clinical Intervention ◽  
Polymer Science ◽  
Medical Sciences ◽  
Prosthetic Devices ◽  
New Horizons ◽  
Biological Source ◽  
Editorial Review

Biomaterials a term is used to describe the materials which are typically derived from any biological source. In generally it is said that these are the materials which are used within the human body to perform certain functions such as therapies1. The applications of polymers in the field of medicine have already gave birth to polymer science as a field. As we can see today, almost every polymer have been reported for use in any kind of clinical intervention, they are inseparable part of us now. Polymers are key players in clinical medicine as they are fundamental components of permanent prosthetic devices such as diameter vascular grafts, artificial lenses, catheters, hip implants etc., and the research is continued to perfect the performance and stability of polymers in vitro and in vivo2. However, the use of polymers in surgery is somewhat confined to connective tissue replacements. Interestingly, polymers have opened new horizons for drug delivery and gene therapy treatments such as nucleic-acid based drugs and protein-based drugs which cannot be taken up as typical pills, are providing impulsion for contemporary implantable polymers. The applications of polymers in tissue engineering are also gaining spotlight as these materials helps in the regeneration of 3D- (three-dimensional) organ and tissue structures.

scholarly journals In vitro three-dimensional modeling of fallopian tube secretory epithelial cells

2013 ◽  
Vol 14 (1) ◽  
pp. 43 ◽  
Author(s):  
Kate Lawrenson ◽  
Maria Notaridou ◽  
Nathan Lee ◽  
Elizabeth Benjamin ◽  
Ian J Jacobs ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Fallopian Tube ◽  
Three Dimensional ◽  
Three Dimensional Modeling ◽  
Dimensional Modeling
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