scholarly journals circCOL6A3_030 is involved in the metastasis of gastric cancer by encoding polypeptide

2020 ◽  
Author(s):  
Wensheng Pan ◽  
Xiaoge Geng ◽  
Jingya Wang ◽  
Chenjing Zhang ◽  
Xiaolu Zhou ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cell Lines ◽  
Human Life ◽  
Pcr Analysis ◽  
Small Peptide ◽  
Node Metastasis ◽  
Distant Lymph Node Metastasis

Abstract Background Gastric cancer (GC) is a serious digestive tract disease that threatens human life worldwide, and the prognosis of gastric cancer accompanied by distant lymph node or the distant metastasis organs is worse. The purpose of this study was to investigate the role of circular RNA COL6A3_030 (circBase ID: hsa_circ_0006401; circRNADb ID: hsa_circ_28198; circBank ID: hsa_circCOL6A3_030) in GC metastasis. Methods qRT-PCR analysis using back-splicing primers and Sanger sequencing of PCR products were performed to identify circCOL6A3_030 in GC tissues and cell lines; RNA-FISH assay was performed to validate the subcellular localization of circCOL6A3_030. Transwell and wound-healing assays were carried out to evaluate the migration ability of GC cells. Western blot was conducted to detect the polypeptide encoded by circCOL6A3_030 in cells. Results circCOL6A3_030 was down-regulated in GC tissues and cell lines, while circCOL6A3_030 was up-regulated in GC with distant lymph node metastasis. The migration of circCOL6A3_030 silenced GC cells was significantly inhibited in both SGC-7901 and BGC-823 cell lines. Importantly, in vivo assay, silencing circCOL6A3_030 could reduce liver metastases from gastric cancer cells. Meanwhile, further studies suggested that circCOL6A3_030 encoded a small peptide that had a function as a tumor-promoting metastasis factor and immunohistochemistry confirmed the presence of this polypeptide. Conclusions To sum up, our study showed that circCOL6A3_030 promoted GC cell migration by encoding a small peptide called circCOL6A3_030_198aa. Therefore, our results highlight the potential role of circCOL6A3_030 for clinical diagnosis and treatment of GC with distant lymph node metastasis.

Role of intraoperative assessment of lymph node metastasis and serosal invasion in patients with gastric cancer

1994 ◽  
Vol 55 (4) ◽  
pp. 250-254 ◽  
Author(s):  
Daisuke Korenaga ◽  
Toshiro Okuyama ◽  
Hiroyuki Orita ◽  
Hideaki Anai ◽  
Hideo Baba ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Serosal Invasion ◽  
Node Metastasis ◽  
Intraoperative Assessment

scholarly journals Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Jing Chen ◽  
Jiakui Zhang ◽  
Xin Li ◽  
Chundong Zhang ◽  
Hongbin Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cell Lines ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Cancer Cell Lines ◽  
Node Metastasis ◽  
Primary Gastric Cancer ◽  
Gastric Cancer Cell Lines

A disintegrin and metallopeptidase with thrombospondin motif type 8 (ADAMTS8), a member of the ADAMTS family, was discovered as a novel angiogenesis inhibitor. We analyzed the expression and methylation of ADAMTS8 in primary gastric tumors and gastric cancer cell lines. We also examined the relationship between ADAMTS8 expression and methylation and clinicopathologic features. The results showed that the significant downregulation of ADAMTS8 mRNA expression was observed in gastric cancer cell lines and tissues, and its expression was related to invasive depth and lymph node metastasis. CpG was hypermethylated in gastric cancer cell lines MKN45, MGC803, and BGC823, as well as primary gastric cancer specimens. ADAMTS8 mRNA expression was significantly lower in methylated primary gastric tumors. A significant association was found between ADAMTS8 methylation status and lymph node metastasis in primary gastric cancer. Moreover, ADAMTS8 expression was upregulated in the gastric cancer cell lines MGC803, BGC823, and MKN45 after treatment with 5-aza-2′-deoxycytidine. Thus, our results demonstrate that expression of ADAMTS8 mRNA is significantly decreased and DNA methylation is frequent in gastric cancer. ADAMTS8 hypermethylation is associated with decreased expression in gastric cancer and may play an important role in the invasion and metastasis of gastric cancer.

Clinical and experimental role of ring finger protein 180 on lymph node metastasis and survival in gastric cancer

2016 ◽  
Vol 103 (4) ◽  
pp. 407-416 ◽  
Author(s):  
J. Deng ◽  
H. Liang ◽  
R. Zhang ◽  
Y. Hou ◽  
Y. Liu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Ring Finger ◽  
Ring Finger Protein ◽  
Node Metastasis ◽  
Finger Protein

scholarly journals Long noncoding RNA CCAT1 rs67085638 SNP contribution to the progression of gastric cancer in a Polish population

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tomasz Olesiński ◽  
Anna Lutkowska ◽  
Adam Balcerek ◽  
Anna Sowińska ◽  
P Piotrowski ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Insertion Site ◽  
Leukaemia Virus ◽  
Node Metastasis ◽  
Melting Analysis

AbstractThe role of the long noncoding RNA CCAT1 NC_000008.10:g.128220661C > T (rs67085638) in the development of colon cancer has been reported. Therefore, we assessed the prevalence of rs67085638 in patients with gastric cancer (GC). We also evaluated the effect of rs67085638 on B-cell-specific Moloney leukaemia virus insertion site 1 (BMI1) transcripts in primary GC and counterpart histopathologically confirmed disease-free margin tissue. Using high-resolution melting analysis, we evaluated rs67085638 frequency in patients with the GC genotype (n = 214) and controls (n = 502) in a Polish Caucasian population. qRT-PCR was used to determine BMI1 transcripts. We observed the trend of rs67085638 association in all patients with GC (ptrend = 0.028), a strong risk of the GC genotype in male (ptrend = 0.035) but not female (ptrend = 0.747) patients, and the association with non-cardia GC (ptrend = 0.041), tumour stages T3 (ptrend = 0.014) and T4 (ptrend = 0.032), differentiation grading G3 (ptrend = 0.009), lymph node metastasis stage N3 (ptrend = 0.0005) and metastasis stage M0 (ptrend = 0.027). We found that significantly increased BMI1 transcripts were associated with the primary GC genotype classified as grade G3 (p = 0.011) and as lymph node metastasis N3 (p = 0.010) and counterpart marginal tissues (p = 0.026, p = 0.040, respectively) from carriers of the T/T versus C/C genotypes. rs67085638 may contribute to increased BMI1 transcripts and the progression and rapid growth of GC.

Distant lymph node metastasis of early gastric cancer

Surgery Today ◽  
1997 ◽  
Vol 27 (7) ◽  
pp. 600-605 ◽  
Author(s):  
Hiroshi Isozaki ◽  
Kunio Okajima ◽  
Tadashi Ichiinona ◽  
Keizo Fujii ◽  
Eiji Nomura ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Early Gastric Cancer ◽  
Node Metastasis ◽  
Distant Lymph Node Metastasis

scholarly journals Role of D2 gastrectomy in gastric cancer with clinical para-aortic lymph node metastasis

2019 ◽  
Vol 25 (19) ◽  
pp. 2338-2353 ◽  
Author(s):  
Xiao-Hao Zheng ◽  
Wen Zhang ◽  
Lin Yang ◽  
Chun-Xia Du ◽  
Ning Li ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
D2 Gastrectomy ◽  
Node Metastasis ◽  
Aortic Lymph Node
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