Expanded Reproduction of Chernozem Fertility in Biological Agriculture

The current stage of development of zonal farming systems in the Central Black Earth region involves the creation of balanced, highly productive, and sustainable agricultural landscapes, maximally adapted to the natural conditions of the region and ensuring the preservation and improvement of soil fertility. The solution to the problem of reproduction of soil fertility in traditional agriculture is associated with the use of a large number of energy-intensive resources and, first of all, irreplaceable ones. However, the level and direction of soil/biological processes are not sufficiently taken into account, whereas they to a certain extent ensure the reproduction of soil fertility. In this regard, the problem of the formation of the scientific foundations of the reproduction of soil fertility through the integrated use of methods of biologization of agriculture in the Central Black Earth Region and the activation on this basis of the soil/biological factor in the long-used chernozems acquires special importance. The purpose of the study is to review the changes in soil fertility indicators in conditions of biological agriculture. The paper presents the results of a study to determine changes in soil fertility indicators in typical chernozem of the Belgorod region (Russia) in a field experiment with various technologies of crop cultivation. The study demonstrates the regularities of the transformation of the fertility of chernozem. In terms of its fertilizing efficiency, compost in the applied norm turned out to be equivalent to a half dose of mineral fertilizers since both variants under consideration provided equivalent increases in the corn yield. The full dose of mineral fertilizers on the background of organic matter turned out to be excessive since its introduction did not contribute to a reliable increase in corn productivity.

Adapting the Form of the sanitary and hygienic characteristics of working conditions to the new Sanitary Law

Introduction. The current Form No. 362-1/y-2001 "The sanitary and hygienic characteristics for the working conditions of an employee having suspicion of an occupational disease (poisoning)" does not meet the requirements of Sanitary Law developed within the framework of the "regulatory guillotine". It complicates the investigation of occupational diseases and carrying out an objective examination of the connection between the disease and the profession. The study aims to analyze the Form for the sanitary and hygienic characteristics (SHC) of working conditions and substantiate proposals for its adaptation to the current regulatory legal acts to objectify the investigation of occupational diseases cases and the examination of the connection between the disease and the profession. Materials and methods. We carried out the expert-analytical study. We analyzed and compared data from Rospotrebnadzor (2001-2020) on occupational morbidity and Rosstat (2014-2020) on employment in harmful and (or) hazardous working conditions. We also studied the SHC (503) submitted for examination of the connection between disease and profession (including forensic medical examination). The structure of the Form for the completeness of the presentation of issues on various aspects of working conditions, the quality of the compilation and content of the SHS was determined in accordance with the current Sanitary Law. The authors evaluated the structure of the Form for the presentation of questions on various aspects of working conditions, determined the quality of the compilation and content of the SHC in accordance with the current Sanitary Law. Results. With a decrease in the number of newly registered cases of occupational diseases over 20 years by more than 3.7 times, the share of workers in harmful and(or) hazardous working conditions in the main types of economic activity decreased in 2020 relative to 2014 in 1,1 time (37.3%). A comparative assessment of the standardized indicators of harmful production factors specified in the SHC and the ones approved in SanPiN 1.2.3685-21 revealed their complete inconsistency. The results of in-depth analysis of 503 SGH allowed us to determine the most typical errors in assessing production factors at workplaces: incorrect assessment of standardized single-digit noise indicators (equivalent sound level A for an 8-hour working day), vibration (equivalent level corrected along the Z-, Y-, X-axes ); lack of data on dust load and the average value of air temperature - THC-index; assessment of the labour severity without considering the technological operations performed, etc. We noted the predominant assessment of working conditions by the main factor developing an occupational disease, without considering the accompanying risk factors that aggravate its effect. We also noted a significant underestimation of the levels when assessing the general transport, technological and technological vibration due to the differences between hygienic standards and the norms adopted in the Methodology for conducting a special assessment of working conditions. SCH contains no information on lighting, biological factor, labour intensity in 67.0-75.0% of cases. Conclusion. We substantiated proposals for improving Form No. 362-1/y-2001 of the SHC of working conditions, considering an electronic form of the document, revising the instructions for filling out the SHC.

Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei

In aquatic animals, the light/dark cycle acts as an important biological factor that influences the entire life cycle. Until present, evidence regarding the regulation of physiological metabolic process under different light/dark cycles is limited in Litopenaeus vannamei. In this study, we mainly investigated the effects of different light/dark cycles (12 h light/12 h dark, 0 h light/24 h dark) on the hepatopancreas metabolism and intestinal microbiota homeostasis in L. vannamei using multiomics techniques. One interesting finding was that the body color of L. vannamei became darker after dark treatment for 8 weeks. Further hepatopancreas transcriptome analysis identified down-regulated genes involved in regulating nutrition metabolism, body-color formation, diurnal rhythm, immune function, hormone levels, and posttranslational modifications. The intestinal microbiota analysis showed that dark treatment-induced alterations in intestinal bacterial abundances in L. vannamei, such as decreased (P < 0.05) relative abundance of Formosa, Demequina, Lutimonas and increased (P < 0.05) relative abundance of Ruegeria, Vibrio, Actibacter, Roseovarius, Ilumatobacter, and Kriegella at the genus level. The microbiota functional analysis demonstrated that the dark treatment mainly increased susceptibility of pathogens, decreased nutrition metabolism, and influenced circadian rhythm. This study indicated for the first time that constant darkness treatment darkened the body color and altered hepatopancreas metabolism and intestinal microbiota homeostasis in L. vannamei, which might give potential clues for improving the productive capacities by changing light/dark cycles in shrimp farming.

Hypoxia and Its Influence on Radiotherapy Response of HPV-Positive and HPV-Negative Head and Neck Cancer

Head and neck squamous cancers are a heterogeneous group of cancers that arise from the upper aerodigestive tract. Etiologically, these tumors are linked to alcohol/tobacco abuse and infections with high-risk human papillomavirus (HPV). HPV-positive HNSCCs are characterized by a different biology and also demonstrate better therapy response and survival compared to alcohol/tobacco-related HNSCCs. Despite this advantageous therapy response and the clear biological differences, all locally advanced HNSCCs are treated with the same chemo-radiotherapy schedules. Although we have a better understanding of the biology of both groups of HNSCC, the biological factors associated with the increased radiotherapy response are still unclear. Hypoxia, i.e., low oxygen levels because of an imbalance between oxygen demand and supply, is an important biological factor associated with radiotherapy response and has been linked with HPV infections. In this review, we discuss the effects of hypoxia on radiotherapy response, on the tumor biology, and the tumor microenvironment of HPV-positive and HPV-negative HNSCCs by pointing out the differences between these two tumor types. In addition, we provide an overview of the current strategies to detect and target hypoxia.

Working conditions and occupational diseases of women in the Republic of Bashkortostan

The main factors of the working environment that harm a woman’s health in the course of work are considered to be physical exertion and functional overstrain, noise, chemical and biological factors, and microclimate. The most unfavorable sectors of the economy in the Republic of Bashkortostan are manufacturing, construction and the agricultural sector, where the largest number of jobs are registered that do not meet the hygienic standards for the above-mentioned production factors, which undoubtedly serves as the main cause of the development of occupational diseases among women. Material and methods.According to the medical records of the inpatient patient, the acts on the case of occupational disease and the registration form No. 30, the etiological causes and the structure of occupational morbidity by industry sectors of the Republic of Bashkortostan among women for 2016–2020 were studied. Results. Among the subjects of the Russian Federation, the Republic of Bashkortostan for the analyzed fiveyear period ranked 25–27th in terms of employment of women in jobs with harmful and (or) dangerous working conditions, and among the 14 regions of the Volga Federal District — 3–5 places. If in the period 2016–2019 among all the primary established occupational diseases from 32.4 to 43.0 % were women, then by 2020 more than half of all occupational diseases (54.7 %) were detected among women. Of the 114 cases of occupational diseases identified to 85 female workers, a third of them worked in health care (32.9 %), a slightly smaller part (30.6 %) — in mechanical engineering (manufacturing) and a fifth — in the agro-industrial complex (21.1 %). The leading place in the structure of occupational pathology of women was occupied by diseases associated with high physical overload and overstrain of individual organs and systems (54.2 %). The reason for the change in the structure of occupational diseases in the region in 2020 there was a new coronavirus infection-Covid-19 in medical workers, which brought diseases from the influence of a biological factor to the second position. Conclusion. The state of working conditions and occupational morbidity among women workers indicates the lack of an effective mechanism for protecting women’s labor and the need to develop effective legislative documents for the protection of their labor and health.

Occupational morbidity in the main industries in the context of the COVID-19 conavirus pandemic

Preserving and strengthening the health of the working population is one of the priority tasks of health care in the Russian Federation, including in the Samara region. Up to 39.7% of the country's population works under conditions that do not meet sanitary and hygienic requirements. Unsatisfactory working conditions are the main reason for the development of occupational diseases among the working population in the Samara region. The workers of medical institutions registered 42 acute occupational diseases with permanent disability, including 13 acute occupational diseases with fatal outcomes from the biological factor "new coronavirus infection caused by the COVID-19 virus."

THE INFLUENCE OF THE STORAGE CONDITIONS OF RAW MATERIALS ON THE PRESERVATION OF THE PROPERTIES OF THE FUR SEMI-FINISHED PRODUCT

This article deals with the problem of preserving the properties of a semi-finished fur product under the influence of a biological factor during the storage of raw materials. The characteristic features of the biodegradation of untreated rabbit skins during storage at elevated temperature and relative humidity are identified and described. A complex characteristic of a semi-finished fur product developed from raw materials of various degrees of microbiological spoilage is given. The author offers a point scale of assessment dynamics of the processes of destruction of fur raw materials and a point assessment of the organoleptic indicators of the semi-finished product was developed. As a result of comprehensive studies, it was found that the degree of damage to the fur raw materials being processed affects the decrease in the stability of the structure and strength properties of the finished semi-finished product.

Current state and trends of occupational incidence in the Samara region

At present, the preservation and strengthening of the health of the working population is one of the priority tasks of health care in the Russian Federation, including in the Samara region. Up to 39.7 % of the country's working-age population work in conditions that do not meet sanitary and hygienic requirements. Unsatisfactory working conditions are the main reason for the development of occupational diseases among the working population in the Samara region. 42 acute occupational diseases with permanent disability, including 13 acute occupational diseases with fatal outcomes from the biological factor «novel coronavirus infection caused by the COVID-19 virus», are registered in the workers of medical institutions. In the Samara region, there is a low detection rate of occupational diseases revealed in the course of preventive medical examinations — 43.08 %, this figure remains below the national average — 59.31 %.

UPDATING THE HYGIENIC CLASSIFICATION OF WORKING CONDITIONS UNDER THE INFLUENCE OF FACTORS OF THE WORKING ENVIRONMENT AND THE LABOR PROCESS

Introduction. The approval of new sanitary norms and rules within the framework of the “regulatory guillotine” made it necessary to revise the hygienic classification of working conditions in terms of hazard, which includes Guide R 2.2.2006-05 - one of the fundamental documents in medicine of labor. Purpose of the study. Updating the classification of working conditions based on a risk-based approach, taking into account new regulatory legal acts, risk factors due to the introduction of modern technologies and forms of labor organization and practices of applying the classification. Results. The classification of working conditions under the influence of chemical, physical factors, the severity and intensity of the labor process has been revised taking into account SanPiN 1.2.3685-21, the classification of working conditions by biological factor has been revised and supplemented. The characteristics of the classes of working conditions have been clarified, the categories of occupational risk by classes according to R 2.2.1766-2003 are indicated. Changes in the structure of the classification are considered, concerning the abolition of the "optimal" classes of working conditions; expanding the classification in terms of the severity and intensity of labor by adding the classes "harmful working conditions - 3.3"; canceling or bringing the indicators of the "hazardous (extreme) working conditions" class in accordance with Federal Law No. 426-FZ and the principles of hygienic classification. Conclusion. A version of a risk-oriented hygienic classification has been prepared, corresponding to the new regulations, modern conditions and forms of work organization and aimed at preserving and improving the health of workers.