scholarly journals Eszett (ẞ; ß)-Glucuronidase, a False Positive of Beta (β)-Glucuronidase: Focus on Plant Genetic Transformation

2021 ◽  
Author(s):  
Jaime A. Teixeira da Silva
Keyword(s):  
Escherichia Coli ◽  
Genetic Transformation ◽  
Reporter Gene ◽  
False Positive ◽  
Google Scholar ◽  
Plant Science ◽  
Uida Gene ◽  
Publication Analysis ◽  
Special Character

Abstract The Escherichia coli beta (β)-glucuronidase gene ( GUS ), coded for by the uidA gene, is a popular reporter gene in plant genetic transformation experiments. As a result of a typographic-type error, leading to confusion between Eszett (uppercase ẞ; lowercase ß), a German special character, and Greek lowercase beta (β), some published papers claimed to have used Eszett (ẞ/ß)-glucuronidase, which does not exist. Attention was paid to the 114 false positive entries, i.e., ẞ/ß-glucuronidase, that were detected on PubMed on July 6, 2021. From the 114 entries, 81 (71.1%) were in papers in the field of plant science. After screening 79 of the full texts, the error was quantified in the article’s location. The error was detected in 100% of abstracts on PubMed and also in 100% of the abstracts on the original journal/publisher websites, while 62.0% of papers had this error in the text (once or multiple times). The origin of these errors is unclear. Given that there are approximately 4000, 1100 and 10,600 hits for this false positive on sciencedirect.com, Springer Link, and Google Scholar, respectively, the quantification of this error based on PubMed suggests that a large and thorough quantitative post-publication analysis of papers claiming erroneously to have used non-existent Eszett (ẞ/ß)-glucuronidase is needed. Importantly, where possible, those errors should be corrected.

scholarly journals Establishment of a Tractable Genetic Transformation System in Veillonella spp.

2012 ◽  
Vol 78 (9) ◽  
pp. 3488-3491 ◽  
Author(s):  
Jinman Liu ◽  
Zhoujie Xie ◽  
Justin Merritt ◽  
Fengxia Qi
Keyword(s):  
Escherichia Coli ◽  
Genetic Transformation ◽  
Shuttle Vector ◽  
Transformation System ◽  
Genetic Studies ◽  
Content Type ◽  
Genetic Transformation System

ABSTRACTWe have constructed the firstEscherichia coli-Veillonellashuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinicalVeillonellastrain. A highly transformableVeillonellastrain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for futureVeillonellagenetic studies.

scholarly journals Eliminating false positives in a qPCR assay for the detection of the uidA gene in Escherichia coli

2013 ◽  
Vol 11 (3) ◽  
pp. 382-386 ◽  
Author(s):  
Richard Kibbee ◽  
Natalie Linklater ◽  
Banu Örmeci
Keyword(s):  
Escherichia Coli ◽  
Quantitative Polymerase Chain Reaction ◽  
Qpcr Assay ◽  
Uida Gene ◽  
Chain Reaction ◽  
Taq Polymerase ◽  
E Coli ◽  
Gene Copies ◽  
Polymerase Chain ◽  
Low Levels

Due to contaminant Escherichia coli DNA present in recombinant Taq polymerase reagents, it is not possible to reliably detect low levels of E. coli in samples using the quantitative polymerase chain reaction (qPCR) assay. Native Taq polymerase was successfully used in this study to detect five uidA gene copies (5 fg of genomic DNA) of the uidA gene.

Glucuronidase Assay in a Rapid MPN Determination for Recovery of Escherichia coli from Selected Foods

1987 ◽  
Vol 70 (1) ◽  
pp. 31-34
Author(s):  
Wallace H Andrews ◽  
Clyde R Wilson ◽  
Paul L Poelma
Keyword(s):  
Escherichia Coli ◽  
False Positive ◽  
Uv Light ◽  
Most Probable Number ◽  
Probable Number ◽  
Rapid Methods ◽  
Glucuronidase Assay

Abstract Glucuronidase is present in most strains of Escherichia coli but absent in most other enteric microorganisms; therefore, an assay for this enzyme is useful for determining the presence of the organism. The substrate 4-methylumbelliferyl beta-D-glucuronide (MUG) is incorporated into either lauryl tryptose (LT) broth or EC medium; the inoculated tubes are then incubated under specified conditions and examined under longwave UV light for the presence of a fluorogenic glucuronidase end product. When compared with the 10-day most probable number (MPN) procedure of AOAC, the LT-MUG and the EC-MUG tests required 24 and 96 h, respectively, and gave comparable mean log MPN values for samples of crabmeat, sunflower kernels, and walnut pieces. However, false-positive and falsenegative reactions were observed with foods tested by both of these rapid methods. Overall, method sensitivity was not compromised by using the LT-MUG rather than the EC-MUG method. Incorporation of 25 αg MUG/mL into LT broth resulted in diminished fluorescence of positive reactions, whereas MUG concentrations of 50 and 100 αg/mL provided decisive fluorogenic reactions.

FALSE POSITIVE IDENTIFICATIONS OF ESCHERICHIA COLI 0157

The Lancet ◽  
1987 ◽  
Vol 329 (8528) ◽  
pp. 333 ◽  
Author(s):  
Hermy Lior ◽  
A.A. Borczyk
Keyword(s):  
Escherichia Coli ◽  
False Positive

scholarly journals Detection of AmpC β-lactamases in Escherichia coli using different screening agars

2019 ◽  
Author(s):  
Evert den Drijver ◽  
Jaco J. Verweij ◽  
Carlo Verhulst ◽  
Joke Soer ◽  
Kees Veldman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Confidence Interval ◽  
Sensitivity And Specificity ◽  
False Positive ◽  
E Coli ◽  
Specificity And Sensitivity ◽  
Positive Results

AbstractThe aim of this study was to determine the performance of both cefotaxime and ceftazidime containing agars on the specificity and sensitivity for chromosomal AmpC-hyperproducing and plasmid AmpC harboring Escherichia coli compared to ESBL-producing E. coli and E. coli without ESBL, pAmpC or cAmpC hyperproduction. Second, we evaluated the influence of adding cefoxitin to these agars for detection of both chromosomal AmpC-hyperproducing and plasmid AmpC harboring E. coli.Four different homemade screening agars with cefotaxime (1mg/L), ceftazidime (1mg/L), cefotaxime (1mg/L) with cefoxitin (8mg/L), and ceftazidime (1mg/L) with cefoxitin (8mg/L) were compared to each other for the identification of AmpC producing E. coli. A total of 40 isolates with plasmid encoded AmpC β-lactamases, 40 isolates with alterations in the promoter/attenuator region of the AmpC gene leading to hyperproduction of the β-lactamase, 40 isolates with ESBL genes and 39 isolates lacking both a AmpC and ESBL genotype were used to test the four agars.The sensitivity and specificity were 100% (95% confidence interval (95% CI) 96.1% to 100%) and 48.1% (95% CI 38.6%-60.2%), respectively, for the cefotaxime agar; 100% (95% CI 96.1% to 100%) and 49.41% (95% CI 39.8%-61.4%), respectively, for the ceftazidime agar; 96.3% (95% CI 89.1% to 99.2%) and 77.2% (95% CI 66.7%-85.2%) respectively, for the cefotaxime with cefoxitin agar; 98.8% (95% CI) 92.6% to 99.6%) and 81.0% (95% CI 70.9%-88.3%) respectively, for the ceftazidime agar with cefoxitin. The main reason for false-positive results were ESBL-harboring strains that grew on various agars; therefore, the specificity of each agar reported here was influenced mainly by the proportion of ESBL isolates tested. In conclusion addition of cefoxitin to cefotaxime and ceftazidime containing agars had little influence on sensitivity, but increased specificity for the detection of AmpC in E. coli.

scholarly journals A Kinetic Model Consideration for the Genetic Transformation Process of Escherichia coli

2005 ◽  
Vol 10 (1/2) ◽  
pp. 67-72
Author(s):  
SHOHREH MASHAYEKHAN ◽  
TADASHI NORIOKA ◽  
MOTOMU NISHIOKA ◽  
MASAHITO TAYA
Keyword(s):  
Escherichia Coli ◽  
Kinetic Model ◽  
Genetic Transformation ◽  
Transformation Process
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