scholarly journals MiR-615 Agomir Encapsulated in Pluronic F-127 Alleviates Neuron Damage and Facilitates Function Recovery after Brachial Plexus Avulsion

2021 ◽  
Author(s):  
Kangzhen Chen ◽  
Lu Ding ◽  
Hua Shui ◽  
Yinru Liang ◽  
Xiaomin Zhang ◽  
...  
Keyword(s):  
Brachial Plexus ◽  
Upper Extremity ◽  
Potential Role ◽  
Functional Rehabilitation ◽  
Interacting Protein ◽  
Brachial Plexus Avulsion ◽  
Function Recovery ◽  
Study Results ◽  
Neuron Damage

Abstract Brachial plexus avulsion(BPA)is a devastating traumatic peripheral nerve injury complicated with paralysis of the upper extremity. We previously reported that leucine-rich repeat and immunoglobulin-like domain-containing NOGO receptor-interacting protein 1 (LINGO-1) has a potent role in inhibiting neuron survival and axonal regeneration after central nervous system (CNS) damage and miR-615 is a potential microRNA (miRNA) negatively regulated LINGO-1. However, the effect of miR-615 in BPA remains to be elucidated. Accumulating evidence indicates that pluronic F-127 (PF-127) hydrogel could serve as a promising vehicle for miRNA encapsulation. Thus, to further explore the potential role of hydrogel-miR-615 in BPA-reimplantation, the present study established the BPA rat model and injected miR-615 agomir encapsulated by PF-127 hydrogel into the reimplantation site using a microsyringe. In this study, results indicated that hydrogel-miR-615 agomir effectively alleviated motoneuron loss by LINGO-1 inhibition, promoted musculocutaneous nerve regeneration and myelination, reduced astrocytes activation, promoted angiogenesis and attenuated peripheral amyotrophy, leading to improved motor functional rehabilitation of the upper extremity. In conclusion, our findings demonstrate that miR-615-loaded PF-127 hydrogel may represent a novel therapeutic strategy for BPA treatment.

scholarly journals MiR-615 Agomir Encapsulated in Pluronic F-127 Alleviates Neuron Damage and Facilitates Function Recovery After Brachial Plexus Avulsion

2021 ◽  
Author(s):  
Kangzhen Chen ◽  
Lu Ding ◽  
Hua Shui ◽  
Yinru Liang ◽  
Xiaomin Zhang ◽  
...  
Keyword(s):  
Brachial Plexus ◽  
Upper Extremity ◽  
Functional Rehabilitation ◽  
Interacting Protein ◽  
Brachial Plexus Avulsion ◽  
Function Recovery ◽  
Study Results ◽  
Neuron Damage ◽  
The Central Nervous System

AbstractBrachial plexus avulsion (BPA) is a devastating traumatic peripheral nerve injury complicated with paralysis of the upper extremity. We previously reported that leucine-rich repeat and immunoglobulin-like domain-containing NOGO receptor-interacting protein 1 (LINGO-1) has a potent role in inhibiting neuron survival and axonal regeneration after the central nervous system (CNS) damage and miR-615 is a potential microRNA (miRNA) negatively regulated LINGO-1. However, the effect of miR-615 in BPA remains to be elucidated. Accumulating evidence indicates that pluronic F-127 (PF-127) hydrogel could serve as a promising vehicle for miRNA encapsulation. Thus, to further explore the potential role of hydrogel-miR-615 in BPA-reimplantation, the present study established the BPA rat model and injected miR-615 agomir encapsulated by PF-127 hydrogel into the reimplantation site using a microsyringe. In this study, results indicated that hydrogel-miR-615 agomir effectively alleviated motoneuron loss by LINGO-1 inhibition, promoted musculocutaneous nerve regeneration and myelination, reduced astrocytes activation, promoted angiogenesis and attenuated peripheral amyotrophy, leading to improved motor functional rehabilitation of the upper extremity. In conclusion, our findings demonstrate that miR-615-loaded PF-127 hydrogel may represent a novel therapeutic strategy for BPA treatment.

Follow-up 26 years after dorsal root entry zone thermocoagulation for brachial plexus avulsion and phantom limb pain

2011 ◽  
Vol 114 (1) ◽  
pp. 196-199 ◽  
Author(s):  
Nestor D. Tomycz ◽  
John J. Moossy
Keyword(s):  
Brachial Plexus ◽  
Upper Extremity ◽  
Pain Control ◽  
Dorsal Root ◽  
Phantom Limb ◽  
Entry Zone ◽  
Dorsal Root Entry Zone ◽  
Brachial Plexus Avulsion ◽  
Root Entry Zone

Brachial plexus avulsion and limb amputation are often associated with intractable chronic pain. Dorsal root entry zone (DREZ) thermocoagulation is an effective surgical treatment for upper-extremity deafferentation pain. The authors describe the clinical follow-up and imaging in a patient who underwent DREZ thermocoagulation 26 years ago for postamputation phantom limb syndrome with associated brachial plexus avulsion. This patient continues to have successful pain control without phantom limb sensation and has never experienced a recurrence of his left upper-extremity pain syndrome. This report lends credibility to the notion that, among ablative neurosurgical pain operations, DREZ thermocoagulation may provide the greatest durability of pain control.

Potential role of antibodies against cardiac Kv channel-interacting protein 2 in dilated cardiomyopathy

2008 ◽  
Vol 156 (1) ◽  
pp. 92-99.e2 ◽  
Author(s):  
Martin Landsberger ◽  
Alexander Staudt ◽  
Sangita Choudhury ◽  
Christiane Trimpert ◽  
Lars R. Herda ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Potential Role ◽  
Interacting Protein ◽  
Kv Channel

The role of neurotrophic factors in genesis and maintenance of mechanical hypernociception after brachial plexus avulsion in mice

Pain ◽  
2008 ◽  
Vol 136 (1) ◽  
pp. 125-133 ◽  
Author(s):  
Nara Lins Meira Quintão ◽  
Adair Roberto Soares Santos ◽  
Maria Martha Campos ◽  
João B. Calixto
Keyword(s):  
Brachial Plexus ◽  
Neurotrophic Factors ◽  
Brachial Plexus Avulsion

scholarly journals Localization of Thioredoxin-interacting Protein (TXNIP) mRNA in Epithelium of Human Gastrointestinal Tract

2003 ◽  
Vol 51 (7) ◽  
pp. 973-976 ◽  
Author(s):  
Yasuo Takahashi ◽  
Yukimoto Ishii ◽  
Akiko Murata ◽  
Toshihito Nagata ◽  
Satoshi Asai
Keyword(s):  
Mucosal Immunity ◽  
Potential Role ◽  
Negative Regulator ◽  
Gi Tract ◽  
Human Gastrointestinal Tract ◽  
Interacting Protein ◽  
Thioredoxin Interacting Protein ◽  
Differentiated Cells

Thioredoxin-interacting protein (TXNIP) is a negative regulator of thioredoxin. However, its role in the gastrointestinal (GI) epithelium is as yet unknown. Using in situ hybridization, we demonstrated that mRNA of TXNIP was differentially expressed in the epithelium of the human GI tract. TXNIP transcript was especially prominent in terminal differentiated cells. TXNIP was also highly expressed in lymphocytes in the lymphoid follicles. Our results suggest a new potential role of TXNIP in the differentiation of epithelial cells and in mucosal immunity of the GI tract.

scholarly journals Thyrotropin-Releasing Hormone-Stimulated Thyrotropin Expression Involves Islet-Brain-1/c-Jun N-Terminal Kinase Interacting Protein-1

Endocrinology ◽  
2004 ◽  
Vol 145 (12) ◽  
pp. 5623-5628 ◽  
Author(s):  
Hiroshi Abe ◽  
Koji Murao ◽  
Hitomi Imachi ◽  
Wen M. Cao ◽  
Xiao Yu ◽  
...  
Keyword(s):  
Potential Role ◽  
Endocrine Pancreas ◽  
Transient Transfection ◽  
Gh3 Cells ◽  
Gene Encoding ◽  
Interacting Protein ◽  
Protein Levels ◽  
Stimulation Of ◽  
Transfection Study

Abstract Islet-brain-1 (IB1)/c-Jun N-terminal kinase interacting protein 1 (JIP-1) is a scaffold protein that is expressed at high levels in neurons and the endocrine pancreas. IB1/JIP-1 interacts with the c-Jun N-terminal kinase and mediates the specific physiological stimuli (such as cytokines). However, the potential role of the protein in the pituitary has not been evaluated. Herein, we examined expression of the gene encoding IB1/JIP-1 and its translated product in the anterior pituitary gland and a pituitary cell line, GH3. We then examined the potential role of IB1/JIP-1 in controlling TSH-β gene expression. Exposure of GH3 cells to TRH stimulated the expression of IB1/JIP-1 protein levels, mRNA, and transcription of the promoter. The increase of IB1/JIP-1 content by transient transfection study of a vector encoding IB1/JIP-1 or by the stimulation of TRH stimulates TSH-β promoter activity. This effect is not found in the presence of a mutated nonfunctional (IB1S59N) IB1/JIP-1 protein. Together, these facts point to a central role of the IB1/JIP-1 protein in the control of TRH-mediated TSH-β stimulation.

Potential role of hematopoietic pre-B-cell leukemia transcription factor-interacting protein in oral carcinogenesis

2014 ◽  
Vol 44 (2) ◽  
pp. 115-125 ◽  
Author(s):  
Seiji Okada ◽  
Tarou Irié ◽  
Junichi Tanaka ◽  
Rika Yasuhara ◽  
Gou Yamamoto ◽  
...  
Keyword(s):  
Transcription Factor ◽  
B Cell ◽  
Potential Role ◽  
Cell Leukemia ◽  
Interacting Protein ◽  
Oral Carcinogenesis ◽  
B Cell Leukemia

scholarly journals Potential Role of Clinical Trial Eligibility Criteria in Electronic Phenotyping

2021 ◽  
Author(s):  
Zhehuan Chen ◽  
Hao Liu ◽  
Alex Butler ◽  
Anna Ostropolets ◽  
Chunhua Weng
Keyword(s):  
Clinical Trial ◽  
Feature Selection ◽  
Potential Role ◽  
Lower Percentage ◽  
Eligibility Criteria ◽  
Study Results

2,719 distinctive phenotyping variables from 176 electronic phenotypes were compared with 57,150 distinctive clinical trial eligibility criteria concepts to assess the phenotype knowledge overlap between them. We observed a high percentage (69.5%) of eMERGE phenotype features and a lower percentage (47.6%) of OHDSI phenotype features matched to clinical trial eligibility criteria, possibly due to the relative emphasis on specificity for eMERGE phenotypes and the relative emphasis on sensitivity for OHDSI phenotypes. The study results show the potential of reusing clinical trial eligibility criteria for phenotyping feature selection and moderate benefits of using them for local cohort query implementation.

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